10:13 Nice video sir, but I would like to correct you at the number of hydrogen pumping from complex III and IV which are 4&2 respectively. Hope you will consider this.😊
note that helped me understand this for Biochem. Glyceraldehyde 3-phosphate (G3P) is an intermediary in glycolysis. Glycerol 3-phosphate is the reduced form of DHAP and participates in this shuttle system. G3P is not the same as glycerol 3-phosphate, I think that's an error in this video but I'm not nearly as well versed as Dr. Andrey so take that with a grain of salt.
For clarification, in glycerol 3 phosphate shuttle.... can we simply change the main focus of electron carrier to FADH that produces 1.5 ATP instead of NADH? although its electron carrier originates in NADH from cytosol, but we can differentiate the NADH (electron carrier) from malate aspartate shuttle that produces 2.5 ATP for clear understanding of shuttle mechanism
+Rickson chelliah in complex 4 ,2 protons are pumpt out and 2 are conected to an o atom to make h2o. so 4 are used in total. and in complex 3 it dependens on how you consider the q coensime. it is a littel diffrent in every textbook. hopt it helpt. and sorry for my english, it is my therd language :{
Thank you Sir, for your effort in this amazing channel. I have Biochemistry final exam topics after 30h, so your work here is like emergency button for my revision session :)
I never knew there was another enzyme within the mitochondrial membrane that was part of the ETC. I only figured something wasn't right when I read that you can get pyruvate from glycerol, and the google images gave me a whole another pathway I never seen before. I'm still kinda confused how this ties with glycerol and gluconeogenesis but I guess there's another video out there that will explain.
FAD+ is a prosthetic group of mitochondrial G-3-P, so when it gets reduced to FADH2, it transfers its H2 to ubiquinone(Q) to form QH2 and gets oxidized again to become FAD+
what are the similarities between the NADH molecules produced from the citric acid cycle and the NADH molecules produced from glycolysis and their electron transfer pathways?
Can someone explain why one mole of glucose through the malate-aspartate shuttle generates 38 ATP while though the glycerol 3 phosphate shuttle only 36 ATP is generated?
in Malate-aspartate shuttle the electrons are transferred to NAD+ making NADH in the matrix hence allowing NADH to go through complex I whereas in G3P shuttle it goes directly to Complex III bypassing I
no, it's GA3P glyceraldehyde, which is intermediate of the glycolysis, here G3P stands for glycerol 3P and is part of this shuttle, the only mistake in this lecture was about complex III pumps out 4 H+ whereas Complex IV pumps out only 2
G3P and GA3P are BOTH abbreviations for glyceraldehyde 3-phosphate. It is clear in the video that he is talking about glycerol 3-phosphate and uses G3P as its abbreviation, however you are right, *by convention* G3P is a shorthand for the glycolysis intermediate, NOT glycerol 3-P.
I love how you reinforce your points constantly, it makes the whole concept a lot easier to understand! Thank you :)
10:13
Nice video sir, but I would like to correct you at the number of hydrogen pumping from complex III and IV which are 4&2 respectively. Hope you will consider this.😊
I can not get over how simple you make everything. All of the information is there, I UNDERSTND IT. I am only passing biochem II because of you
Nothing can be more crystal clear than this!!
Hats off to u sir!👑❤️
This is perfection. I used this video for the mcat many years ago and now I have returned for 1st year.
My new favorite teacher..Thank you so much for such an in-depth explanation Sir
I don’t know how would I pass biochemistry exams without u.. you’re absolutely the best
am so grateful. this is so helpful. Thanks a lot
Awesome explanation but complex III pumps 4 protons and complex IV pumps 2. Just wanted to clarify incase anyone was confused
There should be 4 hydrogen pumped from III, and two hydrogen from IV
That' true if you look at a whole Q-cycle and ½ O2. I think he chose ½ of a Q-cycle (so one QH2) and one O2 :)
I'm so glad I found your channel. Your videos are the best. Thank you so much ❤️
extremely helpful!!! understanding concepts like this makes me love biochemistry much much more!
I can not thank you enough for saving my dumb ass. Didnt understand shit in the lecture, it's all SO easy now. Thank you so so much !!!!!
note that helped me understand this for Biochem. Glyceraldehyde 3-phosphate (G3P) is an intermediary in glycolysis. Glycerol 3-phosphate is the reduced form of DHAP and participates in this shuttle system. G3P is not the same as glycerol 3-phosphate, I think that's an error in this video but I'm not nearly as well versed as Dr. Andrey so take that with a grain of salt.
Acronym for Glyceraldehyde 3-phosphate is usually GAP or GADP as opposed to G3P.
Wow, I learn so much from these! Before I had no clue at all how the shuttle system for NADH worked.
You're a beast! Thanks for explaining this better than my prof! Cheers!
Outstanding lectures on all of glycolysis! Thank you!
You're a gem! A+ professor!
This video was really clarifying - thank you!
For clarification, in glycerol 3 phosphate shuttle.... can we simply change the main focus of electron carrier to FADH that produces 1.5 ATP instead of NADH? although its electron carrier originates in NADH from cytosol, but we can differentiate the NADH (electron carrier) from malate aspartate shuttle that produces 2.5 ATP for clear understanding of shuttle mechanism
isn't complex 3 pumping 4 protons into inter membrane space and complex 4 pumping 2 protons??
you are right
+Rickson chelliah in complex 4 ,2 protons are pumpt out and 2 are conected to an o atom to make h2o. so 4 are used in total. and in complex 3 it dependens on how you consider the q coensime. it is a littel diffrent in every textbook.
hopt it helpt. and sorry for my english, it is my therd language :{
Thank you! You are very clear with your lecture!
Why didn't I find you sooner, you would have helped me so much early on T^T I have my exam in 1h30 and I feel somewhat ready, we'll see how it goes.
i would have given up so long ago if it weren't for these videos!!
thank you sooooooo much im doctor (student) from great IRAQ
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طب بغداد ؟؟
I love your videos so much I watch them like a Netflix series lol
Thank you Sir, for your effort in this amazing channel. I have Biochemistry final exam topics after 30h, so your work here is like emergency button for my revision session :)
my professor is really good idk why i end up in youtube;) great exlapanation by the way
thank you very much ,your efforts are highly appreciated !
nice video i am doctor(student) from Ethiopia
You are by far the best biochemistry teacher ever!
i think complex 3 reject 4 H+ and complex 4 reject 2 H+ no?
reject or eject? please clarify :)
may god bless you.. these videos helped me a lot.. :)
you are my hero
I never knew there was another enzyme within the mitochondrial membrane that was part of the ETC. I only figured something wasn't right when I read that you can get pyruvate from glycerol, and the google images gave me a whole another pathway I never seen before. I'm still kinda confused how this ties with glycerol and gluconeogenesis but I guess there's another video out there that will explain.
OMG! Your lectures are the best. Thanks!
You are excellent. Thank you.
5:05 how are they isozymes if they catalyze reverse reactions? Shouldnt they do the same thing?
amazing video! helped a lot, thank you!
Superb lecture ❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤
May You ONLY SEE BLESSINGS !!
until
8:00
Does anyone know how G3P may pass the outer mitochondrial membrane? I don't think it is specified in the video
I had the very same question
@@harirud1851 Me too...
u r awsm.... bst lectures... thnk u sir
Where does the FADH2 go once it is reduced (by the process of G3P being oxidized into DHAP)?
FAD+ is a prosthetic group of mitochondrial G-3-P, so when it gets reduced to FADH2, it transfers its H2 to ubiquinone(Q) to form QH2 and gets oxidized again to become FAD+
@@abhishekkhersa9373 you mean complex 3 receives electrons(or CoQH2) not only from complex1 and 2 but this way(CoQH 2 produced by FADH2)?
I still have no idea what any of this means, but it's much clearer than reading a book haha :)
Thank you sir ... it is very very helpful.
thank you !! Really helpful video
4 H+ are pumped out into intermembrane space at complex 3
Thank you for all the videos! Just to clarify- I thought G3P was Glyceraldehyde -3 -phosphate?
Glyceraldehyde-3-phosphate = PGAL
you are a hero! :)
3 protons are required to synthesize 1 ATP i think not 4 protons
what are the similarities between the NADH molecules produced from the citric acid cycle and the NADH molecules produced from glycolysis and their electron transfer pathways?
Thanks 👍🙏
thank you
you saved me!
all good just one correction. the amount of H+ that are released by complex 3 is 4H+. please pin this comment so the everyone can recheck,
so basically if glycolysis takes place in skeletal muscles then net atp produced is 5 and if it takes place in heart and liver then it is 7...right?
Thank you very much for explaining this so well. Great job! I get it now. :-)
very nice
THANK YOU!!!
Thank you so much sir🙏🙏🙏🙏
Why there were 2H+ pumped out from Complex 3. How is the 2H+ formed?
Thank you sir
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Thank you 😊😊😊😊😊😊😊
Great!
I do not know how can I thank you
Donate a little, I did and highly recommend it. He's donation funded so it's worth it to show a li'l appreciation and keep his lessons afloat
Why isn't there any ads on his videos?
u r amazing
what a fucking boss you are sir
What happens to NADH of link rxn and kreb cycle
thank you :)
this video is fucking amazing
I love you, thanks so ...much...
Can someone explain why one mole of glucose through the malate-aspartate shuttle generates 38 ATP while though the glycerol 3 phosphate shuttle only 36 ATP is generated?
in Malate-aspartate shuttle the electrons are transferred to NAD+ making NADH in the matrix hence allowing NADH to go through complex I whereas in G3P shuttle it goes directly to Complex III bypassing I
3h+ = 1 atp or 4h+ one atp?
GOOD SHIT
Why complex 3 pumps only 2 Proton not 4 because other complex pump 4 Proton
Sorry but isn't G3P used to refer to glyceraldehyde 3P rather than to glycerol 3 P? Thanks in advance :)
no, it's GA3P glyceraldehyde, which is intermediate of the glycolysis, here G3P stands for glycerol 3P and is part of this shuttle, the only mistake in this lecture was about complex III pumps out 4 H+ whereas Complex IV pumps out only 2
Thanks a lot :)
G3P and GA3P are BOTH abbreviations for glyceraldehyde 3-phosphate.
It is clear in the video that he is talking about glycerol 3-phosphate and uses G3P as its abbreviation, however you are right, *by convention* G3P is a shorthand for the glycolysis intermediate, NOT glycerol 3-P.
I think there would be 4 protons from complex 3 and 2 protons from complex 4
Shoutout to my Stony Brook Classmates
Wait a minute... I thought it was 4, 4 and 2 not 4, 2, and 4 protons
thank yew sir
At 10min is proton not ATP to me
I thought Complex III pumps 4 H+ and Complex IV pumps 2 H+
💛💛💛
Legends are still watching thia video in december 2021 for preparing their exams
Like MEEEEE
Something minute, but your G3P structure should be CHOH rather than CH2OH, but awesome vid
thank you sooooooo much im doctor (student) from great IRAQ
Thank you
thank you sir