You Sir deserve a prize! I had to study all that for my medical exam and i learned it so much faster and better than in 1 year at the uni. Seriously man you're my personal hero!
A student from Taiwan , preparing for the Biochem midterm....and OMG this series of videos literally saved my life !!!!! The explanations are so explicit and the summarized notes with colorful graphs are great too!! Subscribed and think i'll stick to these awesome lectures till the end of the semester.
+AK LECTURES (Andrey K) And please don't delete or make them private 😱. This helped me a lot in my 1st year med and I'd like to watch these videos in the future as a course refresher. Thank you so much!!
i have spent the last hours watching all your videos on glucose metabolism. dude, you‘re amazing. i havent found anyone else who explains as well as you do, and especially who explains all the steps in detail. thank you so much for uploading these videos
i appreciate that you have made knowledge accessible to every part of the world. I would love to see the references from where you and your team acquires these knowledge. professors or papers or books, just as a credit and further reading. but what you are doing is reeeeally amazing.
I think I've watched every single video you've made, and I still feel the need to comment on each one because I am so so so appreciative! seriously from the bottom of my heart thank you!!
these diagrams are so so so helpful and his explanations make it so much easier to understand this content. saved me so much time as im currently cramming for exams...
Sir I am in love with ur lectures.every time I see any video I take a screenshot of that video and that become a good notes regarding those topics.thanks sir ..
What is wrong with you - probably the greatest mind ever lived on Earth, stayed virgin and poor / in permanent debt - Nikola Tesla, entirely absorbed with science .
After the H+ ion goes into the half channel and binds onto an aspartic acid of the C subunit, wouldn't it be a neutral charge instead of a positive charge?
THANK YOU SO SO MUCH for your videos, i have watched a number of them and i have learnt and understood everything you said, you explain so well! Thank you so much !
The range of 10 to 14 subunits. Is the reason for the range an optimal health reason? For example in a healthy individual their HLD will be large and 'fluffy' vs smaller. And / or is the variation of 10 - 14 subunits a function of what type of cell the mitochondria resides? Or is it like muscle cells? Sedentary vs weight trained vs marathon trained will have a different profile and average of their number of subunits?
Actually sir aspartic acid is more hydrophilic that's the reason it takes hydrogen and tends to move inner core of matrix which contains water .if wrong please correct sir I hv a doubt??
Great video . Wonder how Nokola Tesla knew this bio-turbine on order to transform eletro-magnetic force to kinetic force . We simply doscover the intricate workings of a grand bio-design .
*I have a question regarding the rotation mechanism.* Andrey says that the hydrophobic Aspartic-acid wants to move to the hydrophobic region of the membrane. But in the same moment that Aspartic-acid is formed, it already is in the hydrophobic part of the membrane, i.e. in the center of "South - North" line through the membrane, is what I think. Or what again is it that I don''t understand?
5:24 "Half channel is open to the matrix of mitochondria" and you shows area above membrane. The matrix however is BELOW the membrane. So it's a mistake.
Not so,, the half channel is open at the inter mitochondrial membrane side as it has to recieve (H+) ions from the side and it then delivers it to the Matrix side,,, the ATP synthase enzyme complex (F0-F1) is actually in a opposite orientation than CF0-CF1 complex of chloroplast.
Thank you sir for making the video...helped me a lot in my desertation programme...one qstn...what happens to the alpha chain in hexamer...as beta chain does all the work whereas alpha chain only captures ATP???
I believe it was just a simple mistake. If in fact, they did rotate in opposite directions then tension would build up along the gamma stalk. Unless there is some unmentioned enzyme that relieves the tension of this opposed rotation, then tho opposite rotation wouldn't be possible to continuously generate ATP through rotational catalysis. He probably just explained it based on the way that it was drawn rather than linking rotation of the F0 portion with the rotation of the gamma subunit.
Short answer The direction of rotation depends on the viewing point of the observer and the reaction catalyzed by the ATP synthase. When synthesizing ATP, and viewed 'from the bottom' (observer faces the intermembrane space looking into the mitochondrial matrix) it rotates clockwise. biology.stackexchange.com/questions/57741/in-which-direction-does-atp-synthase-rotate might be useful for someone else
Complex 5 , Question, in a healthy Mitochondria where the gradient and all other needed factors are optimal, how many 360 degree rotations per second are produced ? Does it matter if it is 10, 11, 12 13 or 14 subunits in circumference?
I can't focus... The teacher is too hot.. :D Just kidding, I can focus and your lessons are very helpful! I hope I'll get an A tomorrow at my biochemistry exam! :)
Epic stuff! I didn't really get something, when the protons binds aspartate and form aspartic acid, how do we regain the proton which goes into the matrix? Actually, does only the proton go or the whole aspartic acid molecule?
aspartic acid lets go of the proton into the matrix. Electron transport chain complex 1/3/4 pumps protons back out into the intermembrane space as the electron moves across the electron transport chain.
So, when the proton binds to aspartate to form aspartic acid, the aspartic acid is in an acidic environment with plenty of H+ ions (intermembrane space). When the c subunits turn to meet the matrix-facing half channel on the a subunit, the aspartic acid has its proton abstracted by the basicity of the matrix and its lack of H+ ions (chemiosmotic pressure). This is seven years late, but I hope this can help someone reading through these comments.
@@mpc7440 thank you for your reply buddy. It is a shame that I don't remember any of that. I hope somebody else reading through the comments finds some benefit here.
my text seems pretty confident that there are only 8 c subunits. be cautious if you use essential biochemistry 3rd edition. it will calculate 2.7 H+ per ATP created.
can anybody explain me why the atp synthase can run reverse and hydrolize ATP into ADP and anorganic phosphate? Ist it because the Protons are only in the matrix site? I have this question in an exam in a few days. Can anybody help me. Pray for me
i dont understand....at the end of this video you say that only the stalk and the C ring rotate, but in the previous video about F1 you say that also alpha3 beta3 rotate 120 degrees.... >.
You Sir deserve a prize! I had to study all that for my medical exam and i learned it so much faster and better than in 1 year at the uni. Seriously man you're my personal hero!
Undoubtedly
Everytime I watch one of your videos, I have at least 3 "lightbulb moments." Thank you so much!
A student from Taiwan , preparing for the Biochem midterm....and OMG this series of videos literally saved my life !!!!! The explanations are so explicit and the summarized notes with colorful graphs are great too!! Subscribed and think i'll stick to these awesome lectures till the end of the semester.
thank you so much sir please never stop making videos you are an amazing teacher :)
+Alana Esty Thank you! I will ! :)
+AK LECTURES (Andrey K) And please don't delete or make them private 😱. This helped me a lot in my 1st year med and I'd like to watch these videos in the future as a course refresher. Thank you so much!!
Thank you...
i have spent the last hours watching all your videos on glucose metabolism. dude, you‘re amazing. i havent found anyone else who explains as well as you do, and especially who explains all the steps in detail. thank you so much for uploading these videos
I have no idea if you still read comments on a 6 year old video but your videos are still saving lives today
You're an absolute treasure, bless you my dude. I love that you also have your notes available too!
Once I get a job I'll donate to your website.
Thanks for all that you do.
Thanks for providing your awesome lectures for free on youtube. This saved my life :-)
Your videos are a huge help for my Biochemistry class.
Also, the people in my study group say you look like Leonardo DiCaprio
Awesome lecture, you save my life. You can absolutely become a perfect Biochem professor !
i appreciate that you have made knowledge accessible to every part of the world. I would love to see the references from where you and your team acquires these knowledge. professors or papers or books, just as a credit and further reading. but what you are doing is reeeeally amazing.
Thank you for explaining things so well!! Your explanations make things make much more sense
I think I've watched every single video you've made, and I still feel the need to comment on each one because I am so so so appreciative! seriously from the bottom of my heart thank you!!
these diagrams are so so so helpful and his explanations make it so much easier to understand this content. saved me so much time as im currently cramming for exams...
Excellent explanation! You are a wonderful teacher!
Thank you so much for these videos!! You have really helped me understand ETC in a deeper way! Please keep making more videos!!!
this man is a god sent from the heavens to save all the poor uni students from failing BCEM
Sir I am in love with ur lectures.every time I see any video I take a screenshot of that video and that become a good notes regarding those topics.thanks sir ..
I hope this guy makes big bucks for this stuff as he is better than all of my professors put together.
What is wrong with you - probably the greatest mind ever lived on Earth, stayed virgin and poor / in permanent debt - Nikola Tesla, entirely absorbed with science .
Your explanation makes it easy to get the concept! Thank you so much🥰🥰🥰
From this video, i am actually able to get the concept clearly...!! What an explanation..!!👏😍
Description is detailed and organized. Thank you!
After the H+ ion goes into the half channel and binds onto an aspartic acid of the C subunit, wouldn't it be a neutral charge instead of a positive charge?
THANK YOU SO SO MUCH for your videos, i have watched a number of them and i have learnt and understood everything you said, you explain so well! Thank you so much !
Thank you very much for your teaching method! It is very helpful in terms of repeating the concept after the illustration!
Thank you!!! Great drawings, wonderful, fluent explanation! You made my day. Let’s be friends!
A student from Nigeria even our lectures watch your videos
iiiii had so much difficulty to understand why it rotates/moves!!! thank you so much
Please keep making videos! So much more helpful than my lectures!
Outstanding teaching skills 🥇
Amazing. Why are you not my biochem teacher?
The range of 10 to 14 subunits. Is the reason for the range an optimal health reason? For example in a healthy individual their HLD will be large and 'fluffy' vs smaller. And / or is the variation of 10 - 14 subunits a function of what type of cell the mitochondria resides? Or is it like muscle cells? Sedentary vs weight trained vs marathon trained will have a different profile and average of their number of subunits?
i really appreciate your hard job, and im really thankful for that. your videos really helpful. God bless you
Thanks a lot.... Your explaining is better than my professor thx again ❤️
Thank you very for the good work you do, you make studying easy
Thanks for this wonderful explanation 👏👏
Actually sir aspartic acid is more hydrophilic that's the reason it takes hydrogen and tends to move inner core of matrix which contains water .if wrong please correct sir I hv a doubt??
this man can teach a 3rd grader the proton movement in ATP synthase lol
Great video . Wonder how Nokola Tesla knew this bio-turbine on order to transform eletro-magnetic force to kinetic force . We simply doscover the intricate workings of a grand bio-design .
it is very useful for my exam, thank you so much!
Saved me for Chem481. Thanks!
Great work! ! Clearly vissualised !
Its Amazing lecture. thank u very much. Please keep making more videos.
just a wonderful explanation... awesome
How can I get this board text?
It’s structure of F0 not F1. You make a slight mistake near 4:30. I love your videos btw. Thank you
*I have a question regarding the rotation mechanism.* Andrey says that the hydrophobic Aspartic-acid wants to move to the hydrophobic region of the membrane.
But in the same moment that Aspartic-acid is formed, it already is in the hydrophobic part of the membrane, i.e. in the center of "South - North" line through the membrane, is what I think. Or what again is it that I don''t understand?
5:24 "Half channel is open to the matrix of mitochondria" and you shows area above membrane. The matrix however is BELOW the membrane. So it's a mistake.
Not so,, the half channel is open at the inter mitochondrial membrane side as it has to recieve (H+) ions from the side and it then delivers it to the Matrix side,,, the ATP synthase enzyme complex (F0-F1) is actually in a opposite orientation than CF0-CF1 complex of chloroplast.
@@RajarshiMahapatra-td4bs : Just read what I wrote and watch what Author shows in time I mentioned.
Thank you sir for making the video...helped me a lot in my desertation programme...one qstn...what happens to the alpha chain in hexamer...as beta chain does all the work whereas alpha chain only captures ATP???
High quality content
This video is amazing, Thank you!
I love this man
these videos are saving my degree.
Unrelated note... what american accent is this?
How does the proton in the up-facing half channel move to the bottom-facing half channel?
If C subunit rotate clock wiz then how g stack rotates counter clock wiz?
I believe it was just a simple mistake. If in fact, they did rotate in opposite directions then tension would build up along the gamma stalk. Unless there is some unmentioned enzyme that relieves the tension of this opposed rotation, then tho opposite rotation wouldn't be possible to continuously generate ATP through rotational catalysis. He probably just explained it based on the way that it was drawn rather than linking rotation of the F0 portion with the rotation of the gamma subunit.
Short answer
The direction of rotation depends on the viewing point of the observer and the reaction catalyzed by the ATP synthase. When synthesizing ATP, and viewed 'from the bottom' (observer faces the intermembrane space looking into the mitochondrial matrix) it rotates clockwise.
biology.stackexchange.com/questions/57741/in-which-direction-does-atp-synthase-rotate
might be useful for someone else
Complex 5 , Question, in a healthy Mitochondria where the gradient and all other needed factors are optimal, how many 360 degree rotations per second are produced ? Does it matter if it is 10, 11, 12 13 or 14 subunits in circumference?
Excellent lec. Thank u sir
Thank you so much , this video is very helpful !!
EXCELLENT 👌
Cutting edge lecture sir
I can't focus... The teacher is too hot.. :D
Just kidding, I can focus and your lessons are very helpful! I hope I'll get an A tomorrow at my biochemistry exam! :)
Always the best
great lecture, thanks
You savin my life, and my biochem grade :D
Epic stuff!
I didn't really get something, when the protons binds aspartate and form aspartic acid, how do we regain the proton which goes into the matrix? Actually, does only the proton go or the whole aspartic acid molecule?
me too
aspartic acid lets go of the proton into the matrix. Electron transport chain complex 1/3/4 pumps protons back out into the intermembrane space as the electron moves across the electron transport chain.
So, when the proton binds to aspartate to form aspartic acid, the aspartic acid is in an acidic environment with plenty of H+ ions (intermembrane space). When the c subunits turn to meet the matrix-facing half channel on the a subunit, the aspartic acid has its proton abstracted by the basicity of the matrix and its lack of H+ ions (chemiosmotic pressure). This is seven years late, but I hope this can help someone reading through these comments.
@@mpc7440 thank you for your reply buddy. It is a shame that I don't remember any of that. I hope somebody else reading through the comments finds some benefit here.
pure gold
my text seems pretty confident that there are only 8 c subunits. be cautious if you use essential biochemistry 3rd edition. it will calculate 2.7 H+ per ATP created.
Do you accept BAT as donation?
Sir which book you consult ?
great video
Thankyou so much sir 🥰🥰
NEVER STOP
you are the best
God bless you
Thank you very much.
Sir please give some lecture on gene cloning and those vector insertion questions and band formation
Ur god sent
isnt aspartic acid hydrophilic? and the reason it seeks to enter matrix is just to escape from the hydrophobic membrane
Why will aspartic acid want to move in hydrophobic portion?Though aspartic acid is more hydrophobic than aspartate it is still hydrophilic
THANK YOU
Thank u so much
i thought the rotation was based on arginine-aspartate electrostatic interactions?
thanx alot .. god bless you
I hear ambulance around 9:49
+Ding Chuan congratulations?
can anybody explain me why the atp synthase can run reverse and hydrolize ATP into ADP and anorganic phosphate? Ist it because the Protons are only in the matrix site? I have this question in an exam in a few days. Can anybody help me. Pray for me
i know it already. Bacteria which have no electron transport chain use this technique to pump the protons back to built a the proton gradient.
i AM LOOOOOOST SO ASPARTATE IN CENTER OF C SUNUNIT AND HALFCHANNEL IN A SUBUNIT??????OR NOOOT
Doesn't complex 3 give 4H+ and complex 4 give 2H+?
i dont understand....at the end of this video you say that only the stalk and the C ring rotate, but in the previous video about F1 you say that also alpha3 beta3 rotate 120 degrees.... >.
You live in a place off Lamborghini noise and police sirens. May be a biohacker place xD haha
ILY
💛💛💛
would you marry me?
I find the constant repetition of "actually" quite distracting. But otherwise this is a useful explanation.