man I'm a med student in Italy, you saved my faliure in exams, thank you, I would consider you as a friend, for real you do something that actually helps people, that's why I think that being a teacher is the most important thing in the world bless!
Hello sir , i am a fan of your teaching style . watching ur videos for two years now and i have reached my last year of graduation , i have got really good marks in the most difficult subjects of biotechnology just by seeing your lectures... After watching every video , i thanked u wholeheartedly for making such detailed and descriptive versions of topic ... You are truly an inspiration sir .. even now i can find anything regarding my entrance exams , you have made it easy for all your students, thank u so much .
Sir, I've been watching ur lectures for the past 3 semesters and since then,my grades have gone up.And now,I'm watching them for the final sem(current cgpa-90)If anything,I owe a lot to u,a huge thanks...😃
Literally hovered over 30 videos to understand the concept of loss of tetracyline resistance but couldn't understand it any where better than here Thank you
Do you know what is special in your lectures .that is a basic thing I found ,which I couldn't find from most of the teachers.first giving hope and preparing us mentally to learn things easily and happily.something like encouraging us"it is easy"I thing I really need to appreciate this.and also you put a diagrams in one view to get a basic idea of the lesson in the begging.Good job.your work is appreciated.
wow! this is powerful, I am doing my Msc programme in Ebonyi State University Nigeria ,you are really making a whole lots of things easier. God bless you.
i am preparing for my gat b exam just watching your videos. seriously never in my gaduation i got so much clear understanding of concepts. thank you Mr. shomu. wish me luck to get admission for masters !
Love your lectures. Since last 2years i have been watching your vedios and I have cleared my genitics, cell biology and molecular biology exams just by your vedios. May god bless u abundantly sir. U r unmatchable. Simply THE GREAT
I had this question today in my exam...as i wrote down the essay on vectors...I was thanking you all the time I wrote it silently...my worst subject went up so beautifully...thnx once again sir...
No one can replace you sir... For me u r an angle.. I share all ur videos to my friends to help them out with there problem.. N allah bless you... And u get all the success in ur life...
THANK YOU SIR..GREAT EFFORT ...HELPED ME A LOT..15YRS AFTER MY MSC I AM APPEARING FOR MY CSIR UGC NET..I GOT CONFIDENCE AFTER YOUR VIDEOS ONLY.....STAY BLESSED..
I often try to give my time to watch ur videos ....... MASHALLAH after watching ur videos i never find any difficulty in understanding the book.....some times i dont fell necessity of book consultation.............All praises for ALMIGHTY ALLAH Who give us such a priceless gift in the form of Mind......
Outstanding Explanation by you....Biology Expert.....I just want to say thanks to u for Simplyfying all the difficult things in your own way... Thankyou so much... I am very thankful for ur Channel.... which helps me a lot...
Seriously superb ....thanks to give your knowledge...it's too good..no words to express truly ..because genetics is very difficult for me .but make it easy .Nd plz share many more videos with us ..thanks
Thank you for the video. However the explanation about the selectable marker is not quite tight. I am referring to the section from minute 11:00 to minute 13:42. I might have misunderstood you. The selection of clone having the recombinant plasmid cannot be explained here. The bigger use for Selectable Marker region is for a different issue: the transformation might not be 100% efficient and bacteria might or might not have the recombinant vector. Before transformation, you have to separate this mixture to obtain a solution that contains only the recombinant vector, which is tranformed to the bacteria (if you have a mixture of both self-annealed vector and recombinant vector to be transformed to bacteria, every bacteria that has either vector will grow in the antibiotic). Whichever bacteria does not have the vector will not grow, whichever bacteria does have the vector will grow, and of course it will have our interested gene. Just something for clarification!
Yeah, unfortunately Somu got this part wrong. I actually was watching this video just to learn about ways to prevent self-ligation of the vector. So far, I know two ways to prevent it. 1) In the ligation reaction, increase the concentration of the insert compared to the vector, like 6:1 or even higher. This increases the propability of vectors ligating with the insert. However, self-ligation is still possible. 2) Treat your vector before the ligation with Alkaline phosphatase. This enzyme will phosphorylate the ends of the (already digested) vector. Phosphate groups are negatively charged and repell each other, thus preventing contact of the vector ends with each other. In any way, to identify if the insert is correctly integrated, an analytic digest is necessary (using restriction enzymes upstream and downstream of the insertion site).
I've been watching your videos for almost 4 years right now, I'm studying biotechnology degree and each time I search for something that I'm trying to study, I find your videos as first results, you are virtually studying with me all of those years HAHAHA, thanks for the help and the efforts that you're putting in those explanations, I really like people like you who always continue to work hard and procude something useful no matter the circumstances.
Shomu this video was excellent....very easily explained that even a non science person like me can understand.......I want to know what does Chinese Hamster has to do in Recombinant technology
Shomu ji, how are you? I hope you are fine in this Corona era. While still find your teaching understandable and refreshing. Keep on doing the teaching.
I have been watching lots of your videos recently (preparing for exams, hahaha) and I must say these are very helpful! Thank you so much. And by the way - this is the first video I saw where you actually show your face, and you look much better than you sound (no offense) :D
He saved my semester here in USA and got >90%, he is exceptional...
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures.
@@shomusbiologyofficial ye boss u doing good stuff
Your videos help me a lot 😭😭😭
@@shomusbiologyofficial I need PDF of this video is it possible
Anafundisha vizuri
man I'm a med student in Italy, you saved my faliure in exams, thank you, I would consider you as a friend, for real
you do something that actually helps people, that's why I think that being a teacher is the most important thing in the world
bless!
If I get my PhD, I owe 60% of all my understanding in science to you, Shomu. Thank you so much.
That's a huge complement. Thank you for watching the lecture
Shomu's Biology Thank you for posting the videos.
Same here, If I get my PhD I will remember you for all my life man!
Nice bro
seriously u r the only last minute saver from exams !!!!!!!!
madhuri suryawanshi yaa true
Pagal hai kya
madhuri suryawanshi n
Ya
I agree
Ur videos saved us!
Huge Respect from AUSTRALIA!
Thank you. Glad you liked my lectures
Not all heroes wear capes. Thank you so much for this engaging and understandable lecture!
Thank you so much for appreciating my efforts
Hello sir , i am a fan of your teaching style . watching ur videos for two years now and i have reached my last year of graduation , i have got really good marks in the most difficult subjects of biotechnology just by seeing your lectures... After watching every video , i thanked u wholeheartedly for making such detailed and descriptive versions of topic ... You are truly an inspiration sir .. even now i can find anything regarding my entrance exams , you have made it easy for all your students, thank u so much .
I am honoured
It have been 5 years and still there are people benefits from this video, I as an example, thank you man, God bless you
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
Sir, I've been watching ur lectures for the past 3 semesters and since then,my grades have gone up.And now,I'm watching them for the final sem(current cgpa-90)If anything,I owe a lot to u,a huge thanks...😃
I am glad to hear that you are getting benefit from my lectures
Literally hovered over 30 videos to understand the concept of loss of tetracyline resistance but couldn't understand it any where better than here
Thank you
Kyu ji ?
Do you know what is special in your lectures .that is a basic thing I found ,which I couldn't find from most of the teachers.first giving hope and preparing us mentally to learn things easily and happily.something like encouraging us"it is easy"I thing I really need to appreciate this.and also you put a diagrams in one view to get a basic idea of the lesson in the begging.Good job.your work is appreciated.
wow! this is powerful, I am doing my Msc programme in Ebonyi State University Nigeria ,you are really making a whole lots of things easier. God bless you.
i am preparing for my gat b exam just watching your videos. seriously never in my gaduation i got so much clear understanding of concepts. thank you Mr. shomu. wish me luck to get admission for masters !
Love your lectures. Since last 2years i have been watching your vedios and I have cleared my genitics, cell biology and molecular biology exams just by your vedios. May god bless u abundantly sir. U r unmatchable. Simply THE GREAT
+RICHA YADAV thank you. Glad you liked my lectures
I had this question today in my exam...as i wrote down the essay on vectors...I was thanking you all the time I wrote it silently...my worst subject went up so beautifully...thnx once again sir...
No one can replace you sir... For me u r an angle.. I share all ur videos to my friends to help them out with there problem.. N allah bless you... And u get all the success in ur life...
Thank you for watching the lecture
Brother you are a life savior.Seriously are you a biology god or something.I owe my biology paper to you❤🖤
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
THANK YOU SIR..GREAT EFFORT ...HELPED ME A LOT..15YRS AFTER MY MSC I AM APPEARING FOR MY CSIR UGC NET..I GOT CONFIDENCE AFTER YOUR VIDEOS ONLY.....STAY BLESSED..
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
I often try to give my time to watch ur videos ....... MASHALLAH after watching ur videos i never find any difficulty in understanding the book.....some times i dont fell necessity of book consultation.............All praises for ALMIGHTY ALLAH Who give us such a priceless gift in the form of Mind......
Ur class was amazing sir.. Am a Msc student if i have any doubt about my subjects i watch ur vedios it is very helpfull😇
Shomu sr u r bestest teachr in the world...☺
Thank you
@@shomusbiologyofficial My pleasure...☺
Thank you for your videos... you have been more helpful than my teachers in this semester!
Outstanding Explanation by you....Biology Expert.....I just want to say thanks to u for Simplyfying all the difficult things in your own way... Thankyou so much... I am very thankful for ur Channel.... which helps me a lot...
Thank you for appreciating my efforts
So simple and clear when u explain. I don't know why these doctor and academic community make the simple matters complex.
u r best biology teacher...plz make ur live videos on cell biology topics n biochemistry topics... thank u so much from deep corner of my heart...
+Alwina Anam thank you.
Shomu's Biology Hey are you from Bengal?
@@shomusbiologyofficial are u from Kolkata.
Love from Pakistan...... Your, all the lectures are very informative. Keep it up...
Thank you so much for appreciating my efforts
Been using your videos since my freshman year, and always you explain well. all the gratitude from ZImbabwe
+Bighton Chinembiri thank you very much. Glad you liked my lectures
Sir donno how to express my gratitude nd love towards you you are my best teacher
Thank you so much for appreciating my efforts
MY FRIEND RECOMMENDED THIS CHANNEL AND ITS EPIC ❤️🔥😎
Thank you for appreciating my efforts
background bird voices especially koyal bird voice in max videos ....make the scenerio so calm....good for learning and study....
Thank you Suman sir
Very very good...You are the best teacher Of biology...God bless you..
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
Seriously Sir, You are a Life saver for each and every biotechnology students thank you so much
Thank you so much for appreciating my efforts
Excited to watch..explaining the idea in simple terms to understand 🤩🤩
Thank you so much for appreciating my efforts
I've passed all 5 semesters till now , its bcos of u only sir thank you so much
Thank you for watching the lecture
Brother u r genius ......u r teaching fabulous....no word cant express your teaching method....
Thank you so much for appreciating my efforts
lots of love from South Africa
Tons and tons of LOVE
Sir your are insane.. your teaching style is out of level…❤
Thank you so much for appreciating my efforts
Sir U R such an honourable person..I owe U so much tqsm for ur wonderful lectures
You're welcome. Glad you like it
Well explained with patience, well done Shomu.I hardly understood the concepts of DNA Recombinant Technology. Thank you.
Thank you for appreciating my efforts
When students during Covid needs the best explaination,Shomu falls down like a thunderbolt😁😁
thank you so much...this is the first video of urs i am watching....it was very helpful...pls continue doing this .....
You are such a great teacher!!! I am learning so much from most of your videos. Very well explained and organized info. THANK YOU
very nice. I am a scientist but I love the way u explain so easily
Glad to hear that you liked my lectures
I wish , you can get 1 million subscriber as soon as possible...bcz u deserve it. ..🤟🤟🤟🤟🤟👌👌👍👍👍👍
Thank you. Glad you liked my lectures
Things that seems too difficult becose easiest after your lecture 👌👌👌✌️shukriya sir
Glad to hear that you're getting benefit from my lectures
Thank you Sir. You should be a professor at our uni. ❤️ great explanation
Thank you so much for appreciating my efforts
Sir I love the way you teach us and I could easily understood all of your videos which is related with my syllabus .I am a zoology student (b.sc)
You're welcome. Glad to hear that you're getting benefit from my lectures
U make very easy for us.... Keep doing this sir... Very good vdo lecture
Thank you so much for appreciating my efforts
This video is really very helpful for me to complete one section ,now i have confidence to score good, thank you so much🙂
Glad to hear that you're getting benefit from my lectures
Thankyou deeply from my heart. The saviour for the last day before exam.
+sanga ATLAS you're welcome
Thank you very much for putting these videos, helped me a lot for basic understanding. ❤
You're welcome
Got around 82 percent in undergrad . By relying 80 percent on ya videos
Seriously superb ....thanks to give your knowledge...it's too good..no words to express truly ..because genetics is very difficult for me .but make it easy .Nd plz share many more videos with us ..thanks
sir thanks ur videos are really ammazing and full of knowledge
God bless u for this goodness
Thank you
U r so powerful to take the class. Thank-you for helping me
You're welcome
thankyou so much its my rdna exam today and your videos are helping me too much ......thanks alot sir
Glad to hear that you are getting benefit from the videos
You are doing a great job. God bless you, Mr Shomu
You r the hero shomu cant belive that you are a phenome thanxx i ll write your name in all my works ❤❤
Thank you so much for appreciating my efforts
Sir g you r the best best best.. I always watch ur video before exam. Nd it’s so informative 😍😍😍plus easily and perfectly explained.
You make such complicated lesson be simple, nice presentation, 😊😊😊
You're welcome. Glad to hear that you're getting benefit from my lectures
you are elixir of life for biology students. Thkuuu sooo much sir
It is really great video! I always search on youtube any topic biology, your channel comes first. you work really hard. Thanks.
I understand from you very good , now adays i study MS genatic engeniring
You're welcome
what a logical and super simple explanation sir .......im proud of u sir
this semester I don't went any tuition and I have no books my only helpful coaching ur channel sir thank u sir for such beautiful teaching style
Glad to hear that you are getting benefit from the videos
yes sir u r really owsm
I am grateful that you exist in this hard world. Thank you so much, u are a live saver
You're welcome. Glad to hear that you're getting benefit from my lectures
Thank you for the video. However the explanation about the selectable marker is not quite tight. I am referring to the section from minute 11:00 to minute 13:42. I might have misunderstood you. The selection of clone having the recombinant plasmid cannot be explained here. The bigger use for Selectable Marker region is for a different issue: the transformation might not be 100% efficient and bacteria might or might not have the recombinant vector. Before transformation, you have to separate this mixture to obtain a solution that contains only the recombinant vector, which is tranformed to the bacteria (if you have a mixture of both self-annealed vector and recombinant vector to be transformed to bacteria, every bacteria that has either vector will grow in the antibiotic). Whichever bacteria does not have the vector will not grow, whichever bacteria does have the vector will grow, and of course it will have our interested gene. Just something for clarification!
Yeah, unfortunately Somu got this part wrong. I actually was watching this video just to learn about ways to prevent self-ligation of the vector. So far, I know two ways to prevent it. 1) In the ligation reaction, increase the concentration of the insert compared to the vector, like 6:1 or even higher. This increases the propability of vectors ligating with the insert. However, self-ligation is still possible. 2) Treat your vector before the ligation with Alkaline phosphatase. This enzyme will phosphorylate the ends of the (already digested) vector. Phosphate groups are negatively charged and repell each other, thus preventing contact of the vector ends with each other. In any way, to identify if the insert is correctly integrated, an analytic digest is necessary (using restriction enzymes upstream and downstream of the insertion site).
I was looking for someone to point this out and you did it, thanks a million!
Wish you were my Rec DNA Tech professor Mr. Shomu !
Thank you. You can always watch my RUclips lectures
I've been watching your videos for almost 4 years right now, I'm studying biotechnology degree and each time I search for something that I'm trying to study, I find your videos as first results, you are virtually studying with me all of those years HAHAHA, thanks for the help and the efforts that you're putting in those explanations, I really like people like you who always continue to work hard and procude something useful no matter the circumstances.
Thank you for appreciating my efforts. Glad to help you out
Hi
Thank u very much sir
What ever I have the knowledge of zoology is just because of u sir thanks again and again sir
Lots of love from kashmir ❤
Glad to hear that you're getting benefit from my lectures. Please subscribe and share
You made this topic very easy.... Thankyou very much sir🙏
Thank you so much sir for spending your valuable time in making these videos 😁
Thank you so much for appreciating my efforts
vivid and simply best video to understand cloning.... thank you
Thank you so much for your videos ! You give excellent explanations . I rely on them for all complicated topics !!
What an amazing explanation, thank you so much🌺
You're welcome
Shomu this video was excellent....very easily explained that even a non science person like me can understand.......I want to know what does Chinese Hamster has to do in Recombinant technology
This is more helpful than my professor which is sad to say but much appreciated.
Thank you. Glad you liked my lectures
nice v nice video
Very good explanation for everything. Hat's off to you please upload video class for insertion vector and replacement vector explanation.
Thank you. Glad you liked
love to watch your lecture sir its a pleasure to be your viewer
Your videos clear all the confusions
You're welcome
Yeah.,.i need this kinda of detail😍 thanks shomu for always being there for students
what a legend! such a logical approach. Thanks from S.A
+Corne Kriek thank you. Glad it helped
Thank u so much sir , your vedeo are very helpful for study and your explanation are very clear
You're welcome. Glad to hear that you're getting benefit from my lectures
Shomu ji, how are you? I hope you are fine in this Corona era. While still find your teaching understandable and refreshing. Keep on doing the teaching.
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
bruh like who ARE YOU and why are you so goddamn smart and on your shit. youre the best
+Sara Alexander thank you. Glad you liked my lectures
Im watching it at 2 am, and now im in search of sleep.
Thats nice video sir👍.
Thank you
I have been watching lots of your videos recently (preparing for exams, hahaha) and I must say these are very helpful! Thank you so much. And by the way - this is the first video I saw where you actually show your face, and you look much better than you sound (no offense) :D
U helped me a lot in understanding lifescience.... Thank you 😇
You're welcome
You are great !!!sir 🙏 thank you for making this topic so much easier.
Glad to hear that you are getting benefit from the videos
Thank you very much. Much love from sri lanka.
You're welcome
Shomus u r are supertalented❤
Thank you so much for appreciating my efforts
U r best .....feel comfortable in learning.
Thank you so much for appreciating my efforts
Thank u so much sirrrrrr for clarifying this topic 👌👌👌👌👌👌👌👌👌👌👌😊😊😊😊😊
Glad to hear that you're getting benefit from my lectures
I saw your clip every day, now i will Fall in love teacher55555
Thank you
Tq u so much sir🙏🙏🙏
U r explain the topic very well......💐💐💐
Really enjoy u r video .......👏👏👏👏👏👏👏👏👏👏
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
Absolutely better than my professor. Thank you so much
You're welcome. Glad to hear that you're getting benefit from my lectures
Video was easy to understand
Thanks a lot Sir
You're welcome. Glad to hear that you're getting benefit from my lectures
I just went from knowing nothing to have a full idea of about the topic. Thank you
Glad to hear that you're getting benefit from my lectures
Amazing brother! U hve crystal clear concepts!
Thank u so so much sir
Ap ki videos se hmari sari problm asan ho jati h
Plz hr topic m ye bhi mention kre k ye kis class k liye h