Gene Cloning technique Part -1 | Steps involved in gene cloning Explained | In Hindi
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- Опубликовано: 16 окт 2024
- In this video I have discussed about what is gene cloning in Hindi, what are the steps involved in gene cloning.
In molecular biology, cloning a gene means inserting the sequence of the gene into a plasmid, which is a small piece of circular DNA that can be "transfected" into a bacterial strain, where the plasmid will replicate to many, many copies. The plasmid carries an antibiotic resistance gene, so when the bacteria are grown in the presence of the antibiotic, only those bacterial cells with the plasmid in them, can multiply.
This allows molecular biologists to easily propagate and grow vast amounts if the gene, and also to manipulate it in many ways, for example to introduce mutations to study its function, etc.
Please watch this video for more detail. If you like this video please like and share this video, and don’t forget to subscribe and click on bell icon for new videos.
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मैम आप बहुत आकर्षक हो.. ❤️
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Mam I have a doubt!!!!
How do we know whether the vector is cloning vector or expression vector??(
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Mam you explained very well and in very simple manner. So thank you mam
Ek baat smjh ni aayi ma'am resistant gene agar vector ke paas hai to wo non transformant ko v survive kara le jayega?
Fir hme transformant kon h kon ni iska pta kse lagega?
You're video so so easy to learning lesson thank you so much ❤❤.I am doing master of science in microbiology . this video is very helpful 👍to study
Eecellent mam aap bahut accha explain krti hu
Mam, jis plasmid me Gene of interest insert hua hoga wo Antibiotic resistance guma Chuka hoga(e.g. ampicillin) Aur jab Ham Sab bacteria Ko Ampicillin me rakhe ge to Recombinant wala to mar jayega to Uska Fayda kya jabki Non Recombinant plasmid , Jisme Gene of interest Insert nahi hua Wo to Dono Antibiotic me grow karega (As in NCERT Book) to hum kese Recombinant plasmid wale bacteria Ko Agal kar sakte he?
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Mam I have a confusion, you said the selection marker is present in the vector (not in the desired gene that are incorporated in the vector), then is it not possible that those bacterial cells in which recombination was not occurred that can also be survived as you said the antibiotic resistant gene is present in the vector, not in that gene which was ligated to it????
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Ma'am, how can we separate the fragment of DNA which has Gene of interest, from the other fragments? Is it done by gel electrophoresis?(I am referring to the topic taught at 4:40 in this video
Yeah by gel electrophoresis...
As we know that DNA having -ve charge so we put in as electrical field within a medium of agrose and strain with dye i.e. ethidium bromide and it visible only by passing it by UV rays so the DNA fragments (small) were goes towards the Anode and (Large ) fragments were at the cathode..
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Plz within 2days ☺️☺️