Gel Electrophoresis | Agarose Gel Electrophoresis Lab Procedure
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- Опубликовано: 3 окт 2024
- #BaaYo
Gel electrophoresis is a method to separate biomolecules.. This property depends upon the shape and weight of the molecule to be separated. To separate different kind of molecules, different sorts of gels are prepared. Like, agarose gel to separate DNA and RNA molecules. Depending upon the size, the concentration of the gels are increased or decreased. Thus in order to resolve smaller DNA/RNA samples high concentration agarose gels are prepared. If you want to differentiate between two molecules which are bigger or smaller by very few units of nucleotides, the size of the gels and concentration are increased as in case of simple sequence repeat markers. Nucleotides, due to their negative charges (presence of phosphate) migrate from negative to positive charge.
Separation of protein molecules require sophisticated PAGE Gels (Polyacrylamide)
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Glycerol to heavy kr dega sample ko to fir vo movment kese krega humara DNA , DNA bands last tak pahunch hi nhi payega,,,,mujhe janna hai glycerol qq add kr rhe hai jb vo DNA ko last tk lekr hi nhi jayega
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Here i have a question. Why we nee 1% or 4% gel.??? I know how to make 1% or 4% gel but why we make 1%or 4% gel.
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