What are restriction enzymes
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A restriction enzyme (or restriction endonuclease) is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites.[1][2][3] Restriction enzymes are commonly classified into three types, which differ in their structure and whether they cut their DNA substrate at their recognition site, or if the recognition and cleavage sites are separate from one another. To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix.
These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses.[4][5] Inside a prokaryote, the restriction enzymes selectively cut up foreign DNA in a process called restriction; while host DNA is protected by a modification enzyme (a methylase) that modifies the prokaryotic DNA and blocks cleavage. Together, these two processes form the restriction modification system.[6]
Over 3000 restriction enzymes have been studied in detail, and more than 600 of these are available commercially.[7] These enzymes are routinely used for DNA modification in laboratories, and are a vital tool in molecular cloning.[8][9][10]
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Hi Suman bro ... ! Krishna here from Bombay in 3rd yr bsc biotech. What an Awesome video bro.. !Expained it so wonderfully, slowly and clearly. Cannot expect a better explanation buddy.Great work.Nice of you to share the knowledge..help others.. Respect for u Mahn .. Thumbs up :).. I along wid friends Followd allmost all ur videos for the exams..as Lecs in college don't help much due to hapazard incomplete and careless teaching. :/ understood fully and better after seeing ur videos. U will be respected every where bro.. Thanks..Owe u for ur efforts ..Cheers from Bombay..
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damn! im in 9th grade and i have to learn this crap!
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the way u teach is the best u actually simplify the difficult concepts thanku so so much
great video!!!!!!.thank you sir.i have been preparing for NTS exam and I am sure that this will definitely help me.thanks alot once again
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‘A bacterium’*
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I was in such a confusion and had so many doubts on this topic. This video was like some blessing 🌸🌸
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you have a way of making concepts easier, thank you.
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Great video! Very helpful!
Some people make molecular biology too complicated but you made it so easy 😢❤
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very nice vid, great explanation
That was a great video. thank you
Honestly you are an excellent guy...I know how the DNA molecule becomes straight after a while..... seriously you guys are amazing teachers....I saw a few RUclipsrs and you're great and I am sure you can only have this kind of commentaries.... I love scientist that post their notes....I believe we can see always another angle from somebody else and I like that.....and always an enriching one too.
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well i want to understand this specific topic bcz my base is very weak in Biotechnology...you did it just fine... thanks bro
good n digestible lecture
thanks for the lecture great one but what i know is EcoR1 cleaves G A not A T, it is Sma1 that cleaves A T creating blunt ends while the latter creates sticky ends
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i want to know according to what the enzyme has its specific seq. of nucleotides which it can recognize it ?
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Really good tutorial
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there great videos helpful to all
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Very good explanation.
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That was a great video
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@Shomu's Biology Sir what if the restriction enzymes created by bacteria are Isoschizomers?
(Isoschizomers can recognise n cleave methylated dna sequences)
Thankuu broo for explaining ....and I think it would be still more useful for competitive exams if u explain the nomenclature of some more enzymes... Plz please try to upload one more video for explaining some more examples so that it will be useful a lot... Even me and my friends almost many of us listen to ur classes thanks bro for explaining so clearlyyyy....
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Superb.... Sir... This video cleared my doubt. Tnq sir
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sir i hav exam exams tomo so could u pls make a video on restriction mapping
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thank u so much, i learned more about restriction enzymes
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sir..need video on pcr troubleshooting
explained well
nice bro.ur the best
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I thought I might make a comment for all those who are like me and don't watch videos that much the whole way through in general, this one about endonucleases: an endonuclease is an enzyme which cleaves a chain of linear polymers comprised of nucleotide units bonded together (polynucleotides) by separating nucleotides except for those on the periphery.
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Sir.Very nice.but I have one doubt.how restriction endonuclease enzyme do not affect our parental genome.?.You say there is methylase in parental genome..But phage genome also have methylase ..But how is specifically affect the phage genome not bacterial genome.i am really confused that area?Can u pls explain?
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great video...
Best explanation sir.. I lv ur vdo
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Excellent vdo sir🙂
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Sir what about the phage genome it would not function anymore??
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Sir - please make video on enzyme technology
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I would like to you to make one video on how to upload the Genomic Sequences at NCBI.
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hii ,sir
R.E is only the restriction endonuclease?? nothng more??
can't be othr nucleases lyk exo? or res.endonuclease??
there are other endonucleases ,but R.E are the most common.
Exo is not cut a correct cleavage it will cut diffrent area so these no use in R.E
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Sir msc biotechnology k bad kya future options available hai?
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Even if it has like a written code for a recognition signal sequence.
How do restriction enzyme know how to do its job? How does it know where to go?
+kenectic it doesn't GO anywhere , its everywhere , the DNA produce so much it fells the hall place of them , swimming everywhere , .
you dont be thank for watching ...im thanking you for explaining
very good video sir .. i am fan of you
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Sir please make a vedio of poly nucleotide Kinase
Great sir g...!
Impressive
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Excellent.
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very good awesome lec bro........EXPLAIN ME 3 TO 5 AND 5 TO 3 SEQUNCE IN DNA PLEASE
n
M K please learn first spelling of"sequence" befaor taking a big leap to restriction enzymes
Tejas u also have to learn the spelling of before firstly🤣🤣
In nomenclature Roman numeral tells about order of isolation
Very Interesting
Thank you
why Restriction site is added to primer.....
sir cutting pattern of enzymes plz send link
very nice helpfull
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Dhanyavad
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Sir from where the restriction enzymes were formed inside the bacterial cell? Which organelle responsible for this?