DNA replication in Prokaryotes 1 | Prokaryotic DNA replication initiation
HTML-код
- Опубликовано: 28 ноя 2015
- Prokaryotic DNA replication initiation - This DNA replication lecture explains about the DNA replication process in details. This first lecture of this video series is about the replication initiation in prokaryotes. This lecture explains how the DNA replication initiation take place in E. coli bacteria.
In this Video lecture Prokaryotic DNA replication is explained.
DNA replication will be the procedure for producing 2 equivalent reproductions collected from one of first DNA molecule. This kind of natural method takes place in all residing organisms which is the basis intended for natural monetary gift. DNA comprises of 2 strands in addition to each and every follicle from the first DNA molecule acts being a web template to the manufacturing from the secondary follicle, an operation called semiconservative replication. Cell phone proofreading in addition to error-checking things ensure around great fidelity intended for DNA replication.
In a very mobile, DNA replication commences on unique places, or perhaps sources involving replication, within the genome. Unwinding involving DNA at the origins in addition to activity involving completely new strands brings about replication forks increasing bidirectional through the origins. Many proteins are generally associated with the replication fork which often assists when it comes to the particular initiation in addition to continuation involving DNA activity. Almost all conspicuously, DNA polymerase synthesizes the newest DNA with the addition of secondary nucleotides on the web template follicle.
DNA replication can also be performed throughout vitro (artificially, outside the house the cell). DNA polymerases remote through cells in addition to man-made DNA primers can often initiate DNA activity on regarded sequences in a very web template DNA molecule. The actual polymerase cycle response (PCR), a typical clinical method, cyclically does apply such man-made activity for you to increase a selected goal DNA fragment at a pool area involving DNA.
The particular initiation associated with DNA replication can be mediated by DnaA, the healthy proteins which binds into a region with the foundation known as the actual DnaA package. In Elizabeth. coli, you will discover four DnaA cardboard boxes, every one of which usually boasts a remarkably conserved 9 bp general opinion sequence 5' -- TTATCCACA -- 3'. Joining associated with DnaA to this particular region will cause that being in a wrong way supercoiled. Third ,, a region associated with OriC upstream with the DnaA cardboard boxes (known seeing that DnaB boxes) grow to be melted. There are three of those locations, and every single can be 13 bp prolonged, and AT-rich (which helps shedding since a smaller amount energy is necessary to bust the 2 hydrogen provides which type between A new and Testosterone levels nucleotides). That region gets the general opinion sequence 5' -- GATCTNTTNTTTT -- 3'. Burning with the DnaB cardboard boxes involves ATP (which can be hydrolyzed by DnaA). Subsequent shedding, DnaA utilizes the hexameric helicase (six DnaB proteins) to be able to complete opposite finishes with the melted DNA. This is where the replication derive may type. Recruitment associated with helicase involves six DnaC meats, every one of which can be attached with one subunit associated with helicase. The moment this kind of sophisticated can be created, an extra several DnaA meats emergency for the authentic several DnaA meats in order to create several DnaA dimers. DnaC can be subsequently unveiled, plus the prepriming sophisticated can be finish. In order for DNA replication to continue, SSB healthy proteins is necessary to stop the sole strands associated with DNA from being created almost any second houses and also to reduce them from reannealing, and DNA gyrase is necessary to alleviate the load (by producing damaging supercoils) produced by the actual motion associated with DnaB helicase. The particular relaxing associated with DNA by DnaB helicase makes for primase (DnaG) and RNA polymerase to be able to perfect every single DNA theme to ensure that DNA activity may initiate.
DNA replication by Suman Bhattacharya
For more information, log on to-
www.shomusbiology.com/
Get Shomu's Biology DVD set here-
www.shomusbiology.com/dvd-store/
Download the study materials here-
shomusbiology.com/bio-material...
You are amazing ..u started this 7 years ago when there was no trend ..u really did hard work now we understand that consistency really matters 🇵🇰❤️
Thank you
Shomu is not the hero biology students around the world diserve but the one they need.
That's the comment of the day
What the heck this is supposed to mean lame
Excellent video! You posted it in just the perfect time too. I'm studying for my Biochem final exam right now, and this has been super helpful to visualize everything!
Your way of teaching never stopped amusing me, I wish my lecturers teach this way. Thank you for the hard work you put in sir.
Thank you so much for appreciating my efforts to help
Finally, a true teacher has arrived!!! My professors suck! They are little more than technicians. Thank you for explaining that the tension( negative supercoil) resolves itself by "melting the DNA" upstream. This is JUST one example of Shomu's gift for explaining the essence of complicated mechanisms.
+Maria Orsic thank you for appreciating the effort
YOU ARE SUCH A GREAT TEACHER IN MY LIFE WHO TEACH ME ALOTS OF THINKS AND MAIN POINTS
You r the best teacher who thoroughly explaining all things
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
Notes v bhejo sir
the style of talking and personality is so amizing...
Even 3D animation doesn't give us this much clarity of the topics 😭❤ you're a Gem!! I wish you were my professor 🙂 Keep going 🎉
Thank you so much for appreciating my efforts
exactly what i needed man... you are videos are good . most of educational videos have terrible audio and annoying background music . thanks for making it simple
My doubts are getting solved...these videos are of great help!
+Jessica Baa thank you. Glad you liked my lectures
One of the best teacher.... Thank you so much...
Mera bas chale toh mai sabko aapke hi lecture dekhne bolu agar NEET qualify krna hi toh...you are really amazing sir🥰🙌
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
Sir, you are the best one who clears all the doubt! 🙏thank you! ☺
You're welcome. Glad to hear that you're getting benefit from my lectures
u are a boon for biology students. keep it up sir👍
Correction: The melting of DNA reduces twist number therefore it favors negative supercoils, BUT DnaA wraps around the DNA (RRS) in a right handed form. This introduces positive supercoils. Linking number will remain constant, twist will decrease and writhe will increase.
Overall Good View :)
Pouya Amin can you explain please.... i am studying right now and i saw your comment.. can you explain what tou just said?
What is negative supercoils?
@@vinayakpatil2312 it's left handed coiling of DNA
@@sunilpatel2684 ok
Perfect depth of topic , perfect way of explaination......So glad I found this channel at the right time .....
Thank you so much for appreciating my efforts
Its so good to see that you are so fascinated with the things you teach and biology as a whole, you have that hint of smile and satisfaction when you explain anything, kudos to you sir. ( and you're a saviour too)✨
Thank you so much for appreciating my efforts
Dear high profile personality sir i am in love after seen such a great type of lecture May Allah give all the things u wished and Allah give u more success in your life -feild etc
really good!!! I have been watching many of your videos and I can understand perfectly!! great teacher!! thanks
Thank you.
all of the DNA enzymes (dnaA, dnaB, etc) DNA is lowercase and their identification letter is uppercase, it makes a difference.
Your lectures are easy to follow. Thank you sir.
I am so influenced by your teaching style , your teaching method is so good sir , I used to watch your lecture videos which are very helpful for us , I am a Student of Biotechnology and I want to join your class in Kolkata for CSIR NET exam..... Thanks you so much sir for helping us by your videos lecture...🙏🙏
Me too
I am reading garderner, Simmons and snustad. I understand those concepts only after watching your video. Otherwise I will be unable to tie the concepts as beads. And you teach in simple English, which is very useful for students who doesn't know any particular language. Thankyou.
Thank you so much for appreciating my efforts
you have no idea sir,how much your video lectures have helped me.thank you
Thank you so much for appreciating my efforts
As usual the demonstration is very nice.....
U encourage the viewer to learn between the lines...
The definite quality of a born teacher....
Wish u more & more success.
Thank you so much Sir its finally cleared. After reading this 5 times it was not clear but now am fully satisfied.Thank u so much everytime I cleared the topic afrer watching your video.
Glad it helped.
You are a good teacher....god bless you.
I was so confused about this topic. Thanks to this video. Now I understand clearly this process. Thank you. 😊
You're welcome. Glad to hear that you're getting benefit from my lectures
thanks for these lectures .... i really appreciate your work ... and your teaching technique is nice....
actually now I know the dna replication thank you so much
this is great, I wish genetics professors would watch your videos before the consider a teaching career!
U made this topic very simple for a better understanding, great way of teaching😍
Glad to hear that
thanks for such wonderful explanation.
Thanks for making a clear picture of this concept. WELL EXPLAINED .This video helped me for my SEMINAR preparation .
Thank you so much for appreciating my efforts
You're just incredible sir. Your classes are very useful even now.
Thank you so much for appreciating my efforts
Thank you so much sir for the explanation... It helpd me a lot....i undrstood each nd evry part of the DNA replication process in prokaryotes......u r one of my fav teachr......thanks once again
Glad to hear that you're getting benefit from my lectures
Really great sir!!!!I m extremely thankful to u sir...
I really like ur way of teaching...thnx alot
Thank you sir...i didn't understand the initation process perfectly during some days but after watching ur lecture about initation is wonderful... And now i have a good idea for the initation of replication in dna...of prokaryotes
Glad to hear that you're getting benefit from my lectures
Am from pakistan thank yo so much sir your lecture solve the problem
Your pattern of explanation was outstanding.......
Thank you so much for appreciating my efforts
Thank you so much , god bless you and your familly , from algeria sending blessings to india !
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
Thank you so much sir...ur lectures help me a lot for my yesterday's paper(molecular biology)
You're welcome
Really Helpful. Your presentation is awesome.
Sir .....i wish I had a teacher like you for my younger self
You're welcome
Nice explanation sir...thanku very much
Nice teaching bro.. I can easily understand ...this video... Thank u..
Best lectures on youtube
Superb sir... Hats off to you..!!!
Just a amazing video ,it helps me a lot thank you
Very well explained....Thank you
Life saviour.
Thanks a ton.
Regards.
very nice video
heartlyyyyyy thankful to you sir fr each video......rely great job ....pdhega India tabhi to badhega india.....proud to have u in india
+AKANKSHA GUPTA thank you. Glad you liked my lectures. Please subscribe and share
thnqqq...for the 1st time i got my concepts clear
You're welcome
Thank you, this is very helpful
How can u be soooooo intelligent.... ?😍😍😍😍
Shivangi Singh Gautam mam it is so simple...u can do this ...by hard working...
@@DanishKhan-mf9eo lmao you think you woke huh
Awesome....u made it easy for me...thankyou
Just say wowwwwwww❤️❤️❤️ lots of love for ur teaching style. U are a perfect tutor❤️
Thank you so much for appreciating my efforts
vry nice video..easy learning..thank you
Very well Explained.... thankyu
thank you so much....for uploadig this type of video it helping in last night of exams
+tarun gaur glad to hear that. Please subscribe and share
Thank you so much for explaining such a hard topic so easily 🙏🙏
Glad to hear that you're getting benefit from my lectures
you have done a great job
thankyou soo much for the video.. this is really helpful...
i seriously like all your videos.. its very helpful for my biotech degree...
+Juhi Syed thank you. Please share and like
very useful so far... i was so confused right now I understand initiation step better... you are the best
Thank you so much for appreciating my efforts
Awesome video as you are..thankyou sir for explaining the dna replication process ...keep doing more videos..😍💪👍
Glad to hear that you're getting benefit from my lectures
Nice seminar in Initiation of DNA Replication in prokaryktic
+TEJASWINI TAJANE thank you
Sir, thank you so much for this lecture 👌🙏
You're welcome. Glad to hear that you're getting benefit from my lectures
Thk you sir..great work💯
Excellent explanation of the topic. Thanks for your guidance.
You're welcome. Glad to hear that you're getting benefit from my lectures
Thanks Bro. You are great!!
Great way of teaching, thanks :)
+Mor Za you're welcome
Thanks 😊 it's so clearly explained 🙏
You're welcome
Sir bahut sundar apon nko teachin styl 🙏🏻🙏🏻❤💐💐💐💐💐💐💐
I really understand 🙏🏻🙏🏻
From .....odisha 🙏🏻🙏🏻🙏🏻🇮🇳
Thank you so much for appreciating my efforts
Superb👍🏽
+Raji G Krishnan thank you.
Excellent video. Thank you so much Sir.
You're welcome
Awesome understanding thanks for this video
Thank you. Glad you liked my lectures
Excellent explanation sir...🙏🙏👍Thank you all Doubts are clear and...now Going to watch replication elongation phase..
Glad to hear that you're getting benefit from my lectures
Thank you so much shomu bhaiya..🙏🙏
Pura lockdown mein aap hi mere guru rahe....🙏🙏
You're welcome. Glad to hear that you're getting benefit from my lectures
@@shomusbiologyofficial 🙏🙏
Thank you sir for such a great explanation ..
You're welcome
VERY GOOD TEACHING ..............SIR
Your videos are always helpful...thankyou
You're welcome
great work!
Reallly awesome sir... 👌
+Nikita Jangra thank you.
That will be 'Dna A' which binds to the 9mer region of replictor to start the DNA replication of E.coli
This was so cool. Like my sir can't teach so much without creating ruckus of chaos
Thank you. Glad you liked my lectures. Please subscribe and share
Sir thank u....its was very easy to understand....as i got strucked in few point....with the diagrammatic presentation it was too easyfor me to understand...
Glad to hear that you are getting benefit from the videos
wow I like these lectures so much !!! Pls keep up the good work !!!
You're welcome. Glad to hear that you're getting benefit from my lectures
Yes indeed. Thanks a lot pls keep up ur good work. May you get enough courage to do more and more of these videos. 👍❤️
Excellent video... your presentation is very helpful sir... thank you☺️☺️☺️
You're welcome
Shomu's looking to give the best exploration towards subjec
Thanking you sir
Thank you so much for appreciating my efforts
Sir hats off to your hard work , itne years aur yaha tak aane k liye 1 plus million subscribers.
You will go ahead because nowadays aspirants want such videos as you only can make. 👍
Ek request hai Sir if you get time toh recent research topics se kya question ban sakte hai voh b videos mein cover Kara dena
Thank you. I will try
I am so lucky to see your videos. 😇🤩
I am glad to share
dada u work really very hard... tnq for making things so easy to understand.. its an old video.. but still things are so easy to understand and i watch ur videos on a regular basis... thank u Dada...
Glad you liked my lectures
@@shomusbiologyofficial yes ,,i jst love ur lectures..these lectures are very helpful..
Your style of study is very very nice.thank u sir
You're welcome
amazing videos shomu sir. Thanks you :)
+Komal Yadav glad to hear that. Please subscribe and share
thank you ...wonderful explanation
You're welcome. Glad to hear that you're getting benefit from my lectures
Ultimate bro...
Very informative and methodology of teaching is superb 😍😍
Thank you so much for appreciating my efforts
Amazing... Really appreciative
Thank you