DNA replication in prokaryotes 2 | Prokaryotic DNA replication elongation
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- Опубликовано: 5 окт 2024
- DNA replication elongation in prokaryotes- This DNA replication process lecture explains the second stage of DNA replication process that is elongation and DNA synthesis in prokaryotes. The step-by-step process of DNA synthesis by DNA polymerase enzyme with the help of leading and lagging strand of DNA is explained in this video lecture.
DNA replication will be the procedure for producing 2 equivalent reproductions collected from one of first DNA molecule. This kind of natural method takes place in all residing organisms which is the basis intended for natural monetary gift. DNA comprises of 2 strands in addition to each and every follicle from the first DNA molecule acts being a web template to the manufacturing from the secondary follicle, an operation called semiconservative replication. Cell phone proofreading in addition to error-checking things ensure around great fidelity intended for DNA replication.
The moment priming will be comprehensive, DNA polymerase III holoenzyme will be packed into your DNA along with replication commences. This catalytic process involving DNA polymerase III entails using a pair of metal ions inside the lively web page, plus a region inside the lively web page which could discriminate concerning deoxyribonucleotides along with ribonucleotides. This metal ions usually are basic divalent cations that will guide the 3' WOW begin a new nucleophilic attack onto the alpha dog phosphate on the deoxyribonucleotide along with orient along with support the badly priced triphosphate about the deoxyribonucleotide. Nucleophilic attack by the 3' WOW about the alpha dog phosphate produces pyrophosphate, that's next subsequently hydrolyzed (by inorganic phosphatase) in a pair of phosphates. This specific hydrolysis pushes DNA synthesis for you to achievement.
On top of that, DNA polymerase III ought to have the capacity to distinguish concerning the right way used basics along with improperly used basics. This is attained simply by distinguishing Watson-Crick bottom twos using a dynamic web page wallet that may be contrasting in form towards the composition involving the right way used nucleotides. This specific wallet incorporates a tyrosine remains that will has the capacity to kind vehicle der Waals relationships using the the right way used nucleotide. Also, dsDNA (double stranded DNA) inside the lively web page incorporates a larger important groove along with shallower minor groove that enables the enhancement involving hydrogen bonds using the 3rd nitrogen involving purine basics as well as the next fresh air involving pyrimidine basics. Ultimately, the lively web page makes comprehensive hydrogen bonds using the DNA central source. These types of relationships lead to the DNA polymerase III closing all around a new the right way used bottom. If a bottom will be placed along with improperly used, these kind of relationships couldn't arise on account of disruptions within hydrogen developing along with vehicle der Waals relationships.
DNA will be understand inside the 3' → 5' route, consequently, nucleotides usually are synthesized (or attached to the theme strand) inside the 5' → 3' route. However, one of several parent strands involving DNA will be 3' → 5' even though the other will be 5' → 3'. To unravel this specific, replication occurs within reverse directions. Going towards replication branch, the cutting edge strand will be synthesized in a ongoing fashion, merely requesting a single primer. On the other hand, the lagging strand, proceeding away from the replication branch, will be synthesized within some small fragments known as Okazaki fragments, for that reason requesting quite a few primers. This RNA primers involving Okazaki fragments usually are subsequently degraded simply by RNase H along with DNA Polymerase I (exonuclease), as well as the holes (or nicks) usually are stuffed with deoxyribonucleotides along with closed by the enzyme ligase.
DNA replication by Suman Bhattacharya
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Thanku Bhagwan ji💓🌼😇🌈🤗🙏Thanku sir🙏😇🌈🤗💓🌼
Thank you so much for appreciating my efforts
Instead of only pointing out the minor mistakes in this video, you could also praise this man for explaining difficult subjects so easy for us to understand. Keep up the good work and we will keep the A grade at the biochemistry!!
Thank you so much for appreciating my efforts
True that
Especially without editing anything in just one shot. That's impressive
But wrong is wrong.
@@palakkhandelwal8487 Go and then rewatch the video. It'll be clear thereafter.
Lucid explanation. But there is a small correction I would like to add. The direction of the last primer of lagging strand will be reversed(i.e., 5'--> 3'). :)
Your video's are very knowledgeable as you go deep into the concepts and mechanisms. Love to watch and gain knowledge from your videos.
Glad to hear that you're getting benefit from my lectures
Can't believe it is 7 years old video, 😮so much detailed explanation ❤❤ thanks sir
You're welcome
I don't think that is correct... in the second part of the video the primer in the loop…when you imagine you straighten the loop out... than the primers in both chains will be in the same direction….or…leading strand is 5‘ (on the left) - 3´(on the right) so lagging strand is 3‘ (on the left) than on the end of the loop it should be 5‘ ....but primer on it starts also with 5‘…Still thanks for your videos, your teaching helps me a lot!
Barbora K the part which is incorrect i agree, but the idea is making a loop only in the direction of 5'-3' which justifies the loop formation. Don't bother about the rest loop, the dna polymerase only select the same direction primer. If you're not satisfied with it watch the video which describes it. I was also confused because of this one. But now I've got it.
Major confusion is created
Yes u r correct in this video the direction of primers in the lagging strand is altered
I'm also agree with you after looping both replication direction will be same
Perfect Sir!! You resolve a huge problem I had nerver understanding how the process goes, and now I understand it! Thank you, Thank you, Thank you!
May Allah bless you, secure your knowledge and your simple yet complete teaching methode!
+Kaouthar Najim thank you. Glad you liked my lectures
Meko nhi aaya smj..!! Plss help mee...! Mera test h nd yeh ques aane hi vaala h for sure...! Help me..!
sir i think there is some thing wrong in this lecture please rectify....:)
with your explanation, this mind blowing topic become so easy to understand...thank u for such great content to reply on.!!!
You're welcome. Glad to hear that you're getting benefit from my lectures
ur videos r too helpful for me...when i hv confusion about any topic..i alwys search ur videos....ur speaking is also very fine...u alwys use easy words in ur sentence
This has helped me a lot.Such a great initiative to clear out the doubts of many who are struggling like me.
Glad to hear that you're getting benefit from my lectures
Excellent video! You posted it in just the perfect time too. I'm studying for my Biochem final exam right now, and this has been super helpful to visualize everything!
First part of the video initiation was helpful...But the elongation part is not clear
it is soo well explained!!
Great explanation as always from Shomu Sir!
I hope I master this concept and answer all the questiona from this topic
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I found one mistake, but in common your lessons are helping me a lot (right now) to pass the exams for the magister program. Thank you so much for your hard work!! I found all I needed and even more. Priceless material if you're tired of reading books (temporarily), and there is a need of human touch :)
Your explanation is far more better then my professors....good job keep it up
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Thanku so much brother...u r a best soul...I don't have a best teacher like you....
Thank you sire. You are the real gem for all life science students. Lots of love and respect from assam.
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Thank you so much sir for such a informative video ...i was always afraid of this topic , but you made it like a childhood story
you are a genius sir
Thank you
Aatma tript ho jati h aapse pdne ke baad🙏You are a gem❤️
Directionality of legging strand is not right explained..but over all perfect..helped me to solve a lot of problems...thx u sir
Thank you so much sir
This is so helpful..
Lots of love and respect from Kolkata
Sir there is just no doubt after seeing your lectures..
You're welcome. Glad to hear that you're getting benefit from my lectures
thank u sir yesterday i got my result & i clear molecular biology paper with good marks thank u very much sir your videos are very helpful & i am doing M.Sc. Biochemistry
The direction of the addition of primer is wrong in the lagging strand.
Primers are added as soon as the helicase unwinds and multiple primers are added to that strand from the region of unwinding
.......correction required.
No the direction is correct.
@@bogdanbogdanovich140 after the formation of loop the direction of dna synthesis is still 3 prime to 5 prime that is incorrect. see the video again.
@@kuldeepgupta8521 I'm afraid you don't understand that prime ends are actual ends and not directions. Technically it is correct.
No, in the video it is wrong totally
@@bogdanbogdanovich140 i agree with you
Excellent sir,i have to read the book only, once after watching these videos...too good to understand ,thnku
Glad to hear that you're getting benefit from my lectures
Thank u very much sir ...
Ur teaching method just amazing....👍👌
You're welcome
But thanks for this video because I was totally blank in my class after this but you have cleared me a lot
Well now you know. Thank you. Glad you liked my lectures
Many thanks for all your great videos. your explain is wonderful and that attractive me to watch and watch without stopping.
sir plz check the legging strand and primer direction on this and dna direction also
U made it easier to understand much more 😀❤thank you.....
Glad to hear that you are getting benefit from lectures
frstly, formation of loop isn't clear to me still. n secondly, primers r removed by RNase H n den d gap is fulfilled by DNA pol 1 . dis portion isn't clear.
Primers are removed by DNA pol 1 by it's 5'-3' exonuclease activity.
sir thank you for such a detailed explaination.
You're welcome
Thanq dada.. I think ur 2nd diagram lagging stand loop is more perfect than first 1...
thanku so much sir..now my all doubt clear about elongation process in DNA replication
Sir... Thanku frm core of my heart💝✨
Thank you so much for appreciating my efforts
You explained it so nicely 🥺thankyou sir
You're welcome. Glad to hear that you're getting benefit from my lectures
Formation of loop in the strand is not clear to me, and the direction of the lagging stand is wrong as you described,
But rest of the videos I watched are very easy to understand
Thank you for clearing our concepts sir...
You're welcome. Glad to hear that you're getting benefit from my lectures
This video is so so good and really helpful.
You're welcome. Glad to hear that you're getting benefit from my lectures
your videos is very helpful f or my study ....thank you so much
Firstly I would like to thanks for all your videos but I have a little bit problem in formation of loop on lagging strand plzzz u explain it clearly if u do I am thankful to you...
Sir please make a new video on this topic again , coz now u have more technology by which you will make it more easy to understand for us.
Okay. Sure
Even difficult topics are explained in simplified manner🤗👍good.
Thank you so much for appreciating my efforts
I just wanna be honest...
At the same time you make it easy to understand as well as confusing ( in the loop formation and replication direction ) ...
Loop formation is a 3d concept better visualized with animation
@@shomusbiologyofficial
Ya..
I watched it
Now i can understand how difficult to teach without animation
But you still did good job 👍👍
Link for animation plz
@@sankalpchowdhury1175
Thanks bcz...
Well explained sir, I like your videos 😀
Thank you
Thank you. There are some mistakes, hope i will do it correctly in my exams.
Yeah
DNA is circular in PROKARYOTES
So how primer will bind at terminal site
You are right katta
It is so easy to understand.... Thank you sir
You're welcome
Because of the some writings seen in the bottom of the video, I can't see the figure clearly. Anyway good information you are great your videos are really helping me for my exams . Thankyou Sir
Turn off captions in settings
excellent thank you sir
You're welcome
Why DNA polymerase can't go in 3'-5'direction? Is there anything in it's structure?
Boss তুমি সেরা! ❤️❤️
How leading and lagging strands can form in same direction ???? Direction of primer is opposite to leading strand primer ???
I am able to understand this sir.. otherwise you are fantastic
Tnqq sirr it's very useful 😍
You're welcome
sir I think u are wrong here. Just check the polarity of Lagging strad and the primer added. Primers are synthesized inside the loop.
Sir you did a mistake
In lagging strand the primer is added in complement strand of 5' end .
Means at 5' end of the ssDNA the primer 5' to 3' is added
I don't understand what you are saying
Well explained sir ....Thank u
You're welcome
very very informative video
Thank you
Is it true that there are leading and lagging direction at both strands?I mean in total we have a leading and lagging direction at leading strand & another leading and lagging direction at lagging strands?
thnk u so much sir for these videos its really helpful
+madhusmita pollai thank you. Glad it helped
Really nice👍...but one thing is a bit confusing dat is d dirctn of d arrows of the primers...
nice explanation sir
thankyou sir
You're welcome
Outstanding
Thank you so much sir
You're welcome
Thank you sir for ur teaching
direction of primer in lagging strand not clear
the loop formation is confusing me
I love your tutorial sir
You're welcome
most beautiful video sir
Thank you
You're welcome
nice sir very helpful
You're welcome
everything is good but ...i think some may also need written notes of the whole described process of u sir...like i do..😄😄😄
Try to make notes on your own...it will help you more to remember the process..
Very understandable
Thank you
Sir, I can't understand clearly about the looping in lagging strand
Thank you for the lessons 🙏❤️
You're welcome
Sir circular dna mai bhi loop bnega but kese ,? Pehle breakdown krna hoga dna ko
I think your conclusion is wrong because direction at lagging strand always 3'-5' direction. although loop will form.
Very nice sir
You're welcome
Mindblowing
thank you so much sir this video helps me a lot many confusion were cleared but sir can you tell me what was the direction problem in lagging strand because i confuse there please reply
Very helpful vid ,thnkuu sirrr
ThAnkyoU brother you helped me a lot.....
Please explain lagging strand clearly sir
you have made similar videos on same topic so which one should we watch , the latest on or the earlier ones ???
plz make a video on import of protein in endoplasmic reticulmn
Very helpful
Superb sir....thank u sooomuch
You are just awsm... thank you very much
I think primer in the lagging strand is not drawn in correct direction. its little bit confusing.. will u plz clear it?
Your videos are good andall topics are well explained but voice is tooo low some time many imp point skipped.
Dono strand ki polarity same kese ker dii Aap ny?
Thank you sir..💐
You're welcome
Bro...I didn't get well about the looping of lagging strand......
Rewatch the video and Copy down the notes on this Chapter given in the description box. It'll be clear to you I think
Sir i have a confusion that loop will not formed over becoz bacterial dna is circular so lagging strand will remain circular ,secondly okazaki experiment does not support loop formation...pls make clear my concept
Sir , can u please remove these subtitles as the are really obstructing your precious lecture
Control is from your side
Direction of both lagging strand and its template strand is same here...this creates big confusion...☹️
Thankyou
You're welcome
Thank u sirr❤️
You're welcome
Superb
You're welcome
Hello sir.plz make a video on DNA recombination i .e holliday model.
It's already there in my channel