DNA Replication in Prokaryotes | Elongation and Termination

In this video we have discussed the Elongation and termination process of replication in prokaryotes.
The Elongation process starts immediately after initiation by adding nucleotides to the chain.
The DNA polymerase III drives the Elongation process in both strands.
It works 5 prime to 3 prime end so that in one strand it runs continuously and in one strand it needs to jump back again and again in order to run 5 to -3 prime end.
Then Termination is done by TER sequences and TUS proteins.
#hussainbiology #csirnet #apbiology

[@nainakshisingoriya5441]

Finally I understand replication in prokaryotes by your video 😌 for me it was very difficult topic until I watched your video. Thank u for making this video with easy understanding.

[@sakhashree9723]

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[@hussainbiology]

Hey Sakhasheee that is so adorable of u.... Really glad my stuff works out... Thanks

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Best channel for biology with simple explanation...love your videos ❤

[@hussainbiology]

thanks for appreciation.....

[@lamminhuyenphan6513]

so many details! good illustrations! thanks so much! I also noticed that you have produced videos frequently, thanks for your effort. keep up!

[@hussainbiology]

thanks Lam for appreciation..Glad to know that it helps ✌️

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You can also check here.
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[@ektaprakash4782]

This video was really helpful. It had cleared all my doubts.

[@pranathikonda112]

Amazing explanation! Really loved the way how you explained with all those details

[@hussainbiology]

thanks for appreciation..Glad it helps ✌️

[@umangpandya574]

It's amazing..too much helps me..😊

[@mizafarzanamohamed328]

thank you so much for helping me understand this. one of my fav biology channel

[@hussainbiology]

Thanks Miza for appreciation..Glad to know that it helps ✌️

[@Rahulhimachalii]

Me to... Sir

[@nilaratnadas5246]

The DnaA binds to 9mer and causes unwinding of 13mer but here u say the opposite. Is it by mistake cause the image is right..🤔

[@chetan9163]

Yeah AT RICH region are more likely to be first get seprate because only 2 hydrogen bonds are involved

[@chetan9163]

In between A and T

[@dinam8906]

Thank you so much for your efforts .finally understood

[@jyotikumari23]

Sir your videos, always help me during my revision time. From last 1 year, I am taking help from your videos.
Well done sir.

[@hussainbiology]

Thank you so much Jyoti....It really inspires me to put more videos like that.....
😊

[@jyotikumari23]

Thank you, for this wonderful videos. 😊

[@jyotikumari23]

Yeahh. are you teaching somewhere???

[@hussainbiology]

Nah...I am only here nowhere else...
Are u somewhere or any job??

[@jyotikumari23]

Nops, not in job. But just like you, teaching on youtube and studying for my Exams 😊

[@yashikamittal5095]

Really awesome sir.. complete detail in short videos👌👌👌👌👌👌
Thank you soo much

[@hussainbiology]

thanks for appreciation..Glad it helps ✌️

[@mosokodimichellemawasha6191]

Am going to write my first test(final year) microbiology on Friday 11 aug 2023,and i am certain that i am going to get distinction because of how useful your videos have been .i will comeback for update. Thank you sir❤

[@hussainbiology]

thanks for appreciation..Glad it helps..All the best

[@sheikhnajmanajma4281]

Big Thank u hussian's biology 👍...

[@hussainbiology]

Thanks Najma for appreciation....Glad to know that it helps....

[@barnalisinha4705]

Thank you so much sir ...ur videos help me a lot .... amazing contents ☺😊

[@hussainbiology]

thanks for appreciation

[@neelamkhan4722]

i had never thought this would be so easy.thank you soo much.

[@hussainbiology]

You're welcome 😊

[@irfanwani9795]

Keep posting... people will realize ur genius eventually

[@hussainbiology]

thanks for appreciation

[@HarshithaMGowda-nk9xd]

Thank you soo much sir... This vedio helps me a lot....

[@prakruthihrpreethi5962]

Explain is easily and very good way thank you so much

[@debs2009]

Thanks for ditails information....this video is very helpful for me 🤓😇

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

[@mohdshahwaj5454]

Thank you sir again.... ❤
Your videos are really concept clearing as always...
U r doing great job... 😊

[@hussainbiology]

thanks for appreciation.. Glad to know that it helps ✌️

[@fc192shibism3]

Awesome Video Sir!! Crystal Clear Explanation!! BTW I just noticed that the TER Sequence looks like the Indian Flag...

[@hussainbiology]

thanks for appreciation.... Ah that is great... ✌️✌️

[@bilalbilalahmed3302]

Very good video keep it up ...sir Allah Pak bless you

[@hussainbiology]

thanks for appreciation

[@dfmalik7876]

FIRST TIME I GOT IT
THANKS A LOT
SIR
KEEP IT UP

[@hussainbiology]

thanks for appreciation

[@RavneetKaur-sq6uu]

Thank u sir ....best teacher of biology ♥️♥️♥️

[@hussainbiology]

thanks for appreciation..Glad it helps ✌️

[@shabirhussain5555]

Good work brother. JazakAllahu khair!!!

[@hussainbiology]

Thanks Shabir for appreciation....
I am also Shabir Hussain 😊😊

[@shabirhussain5555]

@@hussainbiology Nice to see that😊😊😊

[@the_explorer_anje8243]

Sir thanku u explain so nicely

[@anjanasharma7691]

Please make video on more topic from molecular biology related to csir net syllabus...your video gives a crystal clear concept n are so informative

[@hussainbiology]

surely

[@sayantansaha53]

Greatest ever explaination sir

[@mahaboobbashashaik5219]

Cocept and clarity is good

[@cabdullahbihi1902]

DNA-A protein binds to the 9- mer region teacher!!!!

[@bhatwasim6741]

Well DONE Shabir Bhai... Keep it up

[@hussainbiology]

Shukriya Wasim Bhai....Bas is tarah support banaye rakhain

[@bhatwasim6741]

@@hussainbiology insha Allah

[@veyenar2953]

sir pwoli

[@WhatsInAName0]

Very helpful!!! Thankyou sir 🙏🏼

[@hussainbiology]

Most welcome!

[@afshinlfc]

Thanks ive understood the Termination of E Coli.....

[@Sizuka_163]

Very impressive way of teaching

[@hussainbiology]

Thanks for appreciation

[@swami6444]

Exalent sir

[@dvenu8064]

Thank you very much sir

[@ZoologyAP]

you are excellent 😊

[@Rahulhimachalii]

Sir thanxx a lot.... For everything... 🥳🙌🏻

[@hussainbiology]

Thanks for appreciation
Glad it helps

[@aaminfaras528]

The best 😍

[@karthikagokulan]

Thank you so much sir.. ☺️

[@hussainbiology]

You are welcome...Keep sharing

[@jaiveersngh1801]

Sir bhut aacha samjaya h

[@tanushree1203]

Thank you sir ❤

[@egbejustine]

Did you say unwinding occurs at the 9mer sequence...but you clearly stated in your diagram that the DUE is the 13mer sequence...where unwinding occurs

[@minusingh5089]

Amazing

[@kkakshasavaliya2491]

According to standard book 'principles of biochemistry' ..it says lagging strand forms a loop to aid the dimeric structure of ploymerase to word run simultaneously on both new strand.... if i study according to ur video ..will it be correct anyway.....?

[@hussainbiology]

Yeah that is when we get more into it and my video is here to make concepts clear .
But if we see that complex dimeric structure forming that would be difficult to put forward here in short video..
Thanks for ur feedback...

[@gargibandyopadhyay7021]

Thanks sir for your videos 😊

[@hussainbiology]

thanks for appreciation..Glad to know that it helps

[@ommayhany3797]

i think there is a mistake on leading strand and lagging strand as it is mention here....cz leading should be mention on 5 prime to 3 prime , as the opposite of 3 prime to 5 prime of parent strand

[@farihasharif975]

Exactly , that's why I'm so confused

[@monaxiy]

It’s right tho, there’s just a turn on the left of the fork, that might be confusing

[@monaxiy]

Aight never mind, just got the the part where it’s wrong, it’s at 4:03, 5’ and 3’ should be switched on the left of the fork

[@bidishakumari7095]

So well explained

[@hussainbiology]

thanks for appreciation

[@ktar6754]

Sir... Read this...
DNA pol3 functions in 5'-3' direction.
My question is.. This function is based on which strand's 5'-3 ?
Is it Parent DNA strand's 5'-3' or new synthesizing strand's 5'-3' ?
Ohh ohh ohh , got it... after....
Thank you sir 😇

[@fromhavenk7112]

love ur vidios....thnk u

[@hussainbiology]

Thanks Aayat for appreciation...Glad to know that it helps.....Keep sharing and supporting 😊

[@fromhavenk7112]

thnkx sir helpd me a lot

[@hussainbiology]

@@fromhavenk7112 That inspires me to put more videos like that.....

[@blockchainguru1379]

Great video, I am surprised why these videos are only these much view, pls promote ur video.

[@hussainbiology]

Yeah , have very less views..
But i assure you once , this channel grows more we will be getting more exponential views.
All Educational videos face the same fate during the nascent stages.

[@hussainbiology]

Thanks for ur concern.

[@surekhamehta9503]

Which textbook do you refer for your videos

[@itoobasit6023]

Thankyu

[@hafizmuhammadrehan074]

What a beautiful video 😆😆😆😆😆

[@hussainbiology]

Thanks brother...

[@lily_lily_00]

Very easy and simple understanding

[@hussainbiology]

thanks for appreciation..Glad it helps....✌️

[@tayyabsulehri439]

Sir plz upload stomach digestion explanation for msc level.

[@khushboobhati824]

great video 👍👍

[@hussainbiology]

Thank you! Cheers!

[@kev1369]

Please make a video on looping of lagging strand

[@abhishekbariha9858]

DNA A binds to 9 mer side not the 13 mer side

[@akankshabhardwaj2877]

Your explanation is too good 👍😌
Plz tell the name of reference book 🙏🙏🙏🙏🙏🙏, so that I can also read it , currently I am reading this topic from Pearson's Concepts of Genetics , but many things are missing in that book .

[@hussainbiology]

Thanks Akanksha... You can read Alberts or Lodish Cell Biology... Both are great....

[@tahirtantary7438]

You r the best....love u

[@hussainbiology]

Thanks for appreciation...glad to know that it helps

[@tahirtantary7438]

@@hussainbiology ...yes bro...fully informative...I like your videos ......my subject is biotechnology so u know it is too deep subject

[@MHT_Official]

Thank you Very much

[@hussainbiology]

You are welcome Haroon..Keep sharing and supporting

[@laxmanhanji6301]

Nice

[@user-bg3nd8cx1t]

Thank u so much!!

[@hussainbiology]

thanks for appreciation..

[@beautyofthoughts2522]

Assalamualaikum sir ...one query ...DUE site both side hoti h DAR Site k ? ...q k phir termination me replication fork dono taraf se ana chahiye tbi termination complete hoga ..😢 please btaiye samajh nii aya ye

[@Sophie23456]

Sir make videos on biochemistry please

[@Godscalling2960]

Hussain ji salam!

[@hussainbiology]

Waliekumaslam Asraya

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Mashallah

[@havzaimtiaz3049]

sir i have a confusion regarding primers on lagging strand....which primer is considerd the 1st primer the one is which nearest to the 5' END OF template or one which is nearest to the 3' end of template lagging strand

[@hussainbiology]

Nearest to the 5 end of Template strand or we can say the primer which is at farthest from Helicase.

[@havzaimtiaz3049]

@@hussainbiology ok sir ...thank u soo mch

[@gulshanthakur3555]

I have a question ,
Leading and lagging strands are formed in different strand or same strand ??? You tell that they are formed in different strand but I have learned somewhere that both the strands have leading as well as lagging strands

[@hussainbiology]

It is the direction of Polymerase what make leading and lagging strands.
When Polymerase Runs on 3 prime to 5 prime Template strand it creates Leading strand and Vice versa.....
So on only one strand we can have leading as well as lagging strand when two Polymerases work on the same strand in different directions...
Here i have shown only one Polymerase working on one strand.

[@gulshanthakur3555]

Actually there are 2 replication fork instead of one as you have shown .
I think that's why I am getting confused why you have not both leading and lagging on same strands .

[@hussainbiology]

@@gulshanthakur3555 that what i said....✌️

[@aishakhondekar8664]

Thnku sir

[@hussainbiology]

Thanks Aisha for appreciation...Glad to know that it helps

[@saisibani]

How many(number of) dna pol lll involved in the process

[@Chelsy_k]

Thanku so much sir

[@hussainbiology]

thanks for appreciation

[@angrybird1432]

Tq so much sir🙏🙏🙏

[@hussainbiology]

You are welcome ✌️

[@WasimAkram-tk9sh]

masallah sir ....well done ...but one query is that the role of Tus protein and two fork (L&R) is not clear in this video...

[@hussainbiology]

TER is just a sequence on DNA...
TUS is protein ,,,, so a specific Orientation of TUS on Ter sequence makes it a replication termination site.
The Replication fork can pass via TER-TUS complex in one direction but this complex stops fork coming from other side( non permissive )...
TUS binds in accordance with the sequence so that Permissive and non permissive faces are correctly oriented.
TUS se he actually termination hoti hai.....
Bas TER sequence sirf batata hai TUS ko kaise orient krna hai..

[@WasimAkram-tk9sh]

@@hussainbiology thanks....sir one request to you ..plz upload on rapid fire topics regarding csir net dec 2018

[@zubairnajjar7410]

Excellant way of teaching ....but no of vedios are quite few..........plus if Ecology vedioes are made that would be your highness

[@AliKhan-vf1yy]

So then tus should have permissive and non permissive sites...and ter is only site for tus to bind there..

[@priyankapradhan267]

Sir,can you please tell us which book did you refer

[@hussainbiology]

Two Molecular Biology Books.
1.Bruce Alberts
2.Lodish
and Biochemistry , Lehninger

[@afrasiyabkhan8681]

Very nice job

[@hussainbiology]

thanks for appreciation..Glad to know that it helps...

[@molecularbiology6486]

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[@supriyabakshi3443]

This helps a lot only difficulty is in the accent

[@soul5626]

Thank you so much

[@hussainbiology]

You are welcome..

[@molecularbiology6486]

ruclips.net/video/lwhR0Aummzg/видео.html

[@saibarsha4614]

Thanx sir

[@hussainbiology]

You are welcome

[@mitamajoomdar9390]

Sir in this section r u cover both elongation and termination too please reply

[@hussainbiology]

Yes Mita

[@mitamajoomdar9390]

@@hussainbiology thank u sir ur way of teaching is owsom please if possible theoricaal portion also includes is side what u explain sir

[@chrislehman921]

What happened when a fluoroquinolone antibiotic disrupts DNA replication in mammalian cell?

[@molecularbiology6486]

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[@deekshasharma7598]

Do u know that u r amazing!!😄

[@hussainbiology]

thanks for appreciation...Glad to know that it helps

[@bio366geethasankar7]

🙏🙏

[@divitchaudhary7765]

nice video! with a little work on your accent, things will be much better. I had a little problem in understanding a few words.

[@hussainbiology]

okay sir

[@Medical.018]

Is this good for MBBS ist year students.

[@hussainbiology]

surely it is.....

[@mehbubayeasmin5592]

Everything is going above my head. What to do😭😭😭😭😭😭😭 I have exam after 5days and dont understand a single topic in molecular biology 😭😭😭😭😭😭😭😭

[@hussainbiology]

read from books first and then u will understand,, InshaAllah

[@biogenomistry6109]

Only one polymerase elongate how... If im not wrong but here you showing 2 different on each strand

[@hussainbiology]

Yes.. Plz see Research papers and articles from NCBI

[@tanu6464]

😊😊😊😊

[@shaguftabashir7165]

Sir kindly comment the book to follow this topic?

[@hussainbiology]

Lodish Cell Biology , Cooper Cell Biology or Alberts Cell Biology...👍

[@Sophie23456]

Or cell biology also

[@hussainbiology]

making

[@pragnatejateja8742]

This is eukaryotic transcription not prokaryotic

[@blessedslave]

It really helps and highly knowledgeable. But I find it's a bit too quick with that accent. But that should not be a problem as we can watch it .75x . Nevertheless great job

[@hussainbiology]

thanks for appreciation...glad to know that it helps

[@usamakhan-dv1lr]

It's better to understand difficult topics in short time from your lecture instead of wasting time on 1 and 2 hours lectures

[@hussainbiology]

thanks for appreciation..Glad it helps..

[@mahaboobbashashaik5219]

Ididnt understand about left and right fork
U said that right fork moves towards right side. Inthink there is no right movement as per ur concept