Mammalian expression vector

[@ruhidyusifov5106]

Universites and professors need to follow Shomu`s way of explanation he is really brilliant. Thank you teacher!

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@samyuktha652]

I really need to thank u, ur way of explanation is great. Ur vedios really helped me

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@Natalia-wd8ye]

Thank you for this video, it's hard to find info on this specific topic in free access and also you explain it well so it's easy to understand :D

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@gayathrig4311]

U r an amazing teacher😊

[@shomusbiologyofficial]

Thank you

[@sanjeevakumar4912]

I request you to make a video on baculovirus vectors. By the way your tutorials are simply amazing

[@KingLewis46]

Poly-A tag sequence is not for protein isolaton!( 8:16 ) poly A tail is not expressed into protein so you can´t used to fish your protein.

[@C080627]

Agreed, the hGh Poly A sequence is used because it can improve mRNA stability and boost translation efficiency. Also, to ensure the polyadenylation of the mRNA. In this case, a tag (hexahistidine tag) sequence will allow for protein purification.

[@MrRawresh]

thank you, was going to say the same thing.

[@dubstepXpower]

Poly histidine tag right?

[@sidrah461]

Plz do a video on bucculovirus expression vector system in a bsc level

[@danielhain40]

Please make more!!!

[@shomusbiologyofficial]

Sure

[@Garcia-elf]

Can you explain what Markov chain Monte Carlo is and how it is applied to genomics??
What does "sample the joint posterior distribution" mean? Thanks

[@amandeeprana4920]

God of life science students 😍

[@shomusbiologyofficial]

Thank you for appreciating my efforts

[@amandeeprana4920]

Shomu's Biology pls make video on phage display sir please

[@sumit-lr1qm]

দুর্দান্ত স্যার।

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@rhythmdancebymanisha9979]

What are the different kinds of vectors used in stable and transient transfection in mammalian cells and plant cells????

[@danielo.3988]

great videos thank you!!

[@shomusbiologyofficial]

+Daniel O. Thank you.

[@subhiksharajan8547]

Please do videos on bpv based vector and insect vector

[@livelovelaugh6405]

Question- when would u use prokaryotic expression vector and when would u use eukaryotic expression vector? Like in what situations is one better than the other?

[@EliasBroeckhoven]

The difference is post translational modifications. Prokaryotic organisms are able to produce the same amino acid sequence as the gene would in eukarytic organisms but the prokaryot would not be able to modify the protein (i.e. glycosylation) necessary for the same functioning in both organisms.

[@berastainisabel9944]

Great video, thank you very much for your clarity!! yet, poly A is not a selection tag, be careful!!!

[@shomusbiologyofficial]

+Berastain Isa thank you. Glad you liked my lectures

[@manaspaul8726]

Sir please make a vedio about T7 promoter based vector

[@freefiresaro83]

Why we are use 3 ori in mac

[@itratzahra2391]

very nice lectures. kindly suggest any book of gene cloning as easy and brief like ur lectures :)

[@amruthamavily]

Itrat Zahra life science path finder publications

[@janakirawool1661]

Is there any video on promoter selection with plasmid pKO1? ..I can't understand that promoter selection in pKO1

[@mayukhroy5393]

Thank You Sir,for such a clear explanation.

[@shomusbiologyofficial]

You're welcome

[@Kaoriocha]

How do transiently transfected plasmid in the cytosol get expressed? I thought the transcriptional machineries is inside the nucleus...

[@darakhshasaleema146]

Why regulatable promoter is used in protein expression vector?

[@shomusbiologyofficial]

To increase or decrease expression

[@anatolyrozhkov851]

Stop, 6:40 why exactly do we need more than one ori?

[@shilabratabanerjee7650]

So that it can be replicated in different hosts

[@ayeshamushtaq9339]

you great man...

[@ren5124]

Just a question. So this expression vector will not need to be integrated into a chromosome via homologous recombination for it to be expressed? It can just be introduced into the nucleus of the mammalian cell?

[@ratty1942]

How does the vector become linear, to be inserted into the genome?

[@shomusbiologyofficial]

If you cut a circular vector at a single point, it will be linear

[@anicatasnimprotity129]

can a mammalian expression vector be 'transformed' into a bacterial cell?

[@shikhapodwal1540]

thanks

[@SWyrick366]

which restriction enzymes are required to clone the mammalian expression vector pMV2 BMPR?

[@kmrashmi6464]

Sir plz make video on cassette vector,,,plz soon

[@ZincAllThumbs]

THANK YOU! :)

[@shomusbiologyofficial]

You're welcome

[@motivator6974]

Plzzz is lecture ky notes dy dyn

[@santoshitekam9752]

Sir please explain N-terminal
p-FLAG-Mac p-FLAG -ATS

[@tejaswiniingle9294]

Please do a video on SCP vector

[@somasaha5485]

Shomu sir mammalian vector ar mammalian system ki aki plzz reply

[@shomusbiologyofficial]

No. The word system is vast and vector is a gene delivery system which is specific

[@imrocknreeling]

Why don't it degraded inside the cytosol? Since mammian cell is very prone to detect foreign particles. Please describe

[@justicemunyoro]

any response?

[@mohammadamir1461]

can you plz hindi lecturers

[@loveyahshayah8855]

😳🧬 gain of function

[@neuromobmob]

I think i love you man xd

[@shomusbiologyofficial]

Thank you

[@mariamacrina573]

U where the same shirt in most of the video

[@justicemunyoro]

wear v where

[@dubstepXpower]

Thanks

[@shomusbiologyofficial]

You're welcome

[@venkatesanmunusamy7319]

Thanks

[@shomusbiologyofficial]

You're welcome