You are seriously amazing. No other videos explain the details that you touch up on. Literally helping me understand my dissertation. I don't know what I would have done without your videos. Thank you!
Thank you so much my favorite biology teacher. Would you please make several videos about snap gene soft ware? It is not only beneficial, but also related to cloning lectures.
pBR322 is a cloning vector and you put it to the expression vector column, whereas pBAD/D- Topo is an expression vector and you placed it to the cloning vector column. It would have been better if you just reduced the font of the expression vector features and put the right vector there. Just by simply looking at this slide is misleading.
I love your videos. They are very informative. Can you please make a video on Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) genes, with its basic concepts, usage and functionality? Thank you!
Sir expression vectors k kon s examples h . Pbr322 . Puc10 . Bacteriophage . Cosmid phasmid. Bac yac ye sb cloning vector k example h ? Plzz reply don't ignore 🙏
When we use E.coli strains for protein expression we use Rosetta which has chloramphenicol resistance Gene which blocks protein production. But since the E.coli is already resistant why we add also chloramphenicol?
Can i meet you in zoom application to explain me an article? My doctor told me to present this article but i don't understand this article, i read it many times but my brain not understand anything unforunatlly! I will pay to you, so please if you can explain it to me💔
You are seriously amazing. No other videos explain the details that you touch up on. Literally helping me understand my dissertation. I don't know what I would have done without your videos. Thank you!
you must be human......and you are seriosuly he;s amazing don't listen to fucking haters...
Jo concept university prof ni achhe se samjhate wo concept aap bahut hi fundamental tareeke se samjhadete ho sir
Thank you
I had no clue how the expression was connected to the central dogma, thank you so much, sir . May God richly bless you.
Thank you
Such a amazing teacher that i have seen in my 23 yrs age 😇🥰
Thank you❤️ ... tomorrow is my exam. ..I was here for overview but ur lectures are great and simple to understand and learn
Glad to hear that you're getting benefit from my lectures
Thankyou so much sir for your dedication towards teaching. You are a gem❤
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
Thank you so much my favorite biology teacher. Would you please make several videos about snap gene soft ware? It is not only beneficial, but also related to cloning lectures.
Okay I will try
@@shomusbiologyofficial You are the best.
Thank you for your great explanation. You made me to understand precisely.
plzz explain your chapter manually...stop explaining on ppt....cz u explains so well on board...
i agree
I too agree
Sir This video was very Helpful.
You're welcome
Nicely explained 👍
Thank you
Very helpful and clear lecture!
Thank you for putting so much time into your tutorials. Great details. Extremely helpful🙏🏻
Thank you sir..... Thank you 🙂🙂🙂🤗
You're welcome
OMG ITS THE BEST STREAMER IN LITHUANIA
Jasna
pBR322 is a cloning vector and you put it to the expression vector column, whereas pBAD/D- Topo is an expression vector and you placed it to the cloning vector column. It would have been better if you just reduced the font of the expression vector features and put the right vector there. Just by simply looking at this slide is misleading.
Amazing tutor... thank you so much for this amazing video...☺ you are doing fab job..
your lectures are really nice. Can you please lecture about plasmid mapping and the direction of the arrows
I love your videos. They are very informative. Can you please make a video on Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) genes, with its basic concepts, usage and functionality? Thank you!
Glad to hear that you're getting benefit from my lectures
Thank yoy for these amazing tutorials!
You're welcome
Very helpful, thanks!
You're welcome
Sir expression vectors k kon s examples h . Pbr322 . Puc10 . Bacteriophage . Cosmid phasmid. Bac yac ye sb cloning vector k example h ? Plzz reply don't ignore 🙏
thank you very much for your videos they very helpful
You could do a video of Promoters used in protein expression (lac, trp, tac, PL, Pt7...) in yeast, Pichia pastori, and baculovirus
Thank you so much
You're welcome
superb sir....thanks
Plz do a video on benzer r2 locus experiment...i m badly in need of ds explaination.
When we use E.coli strains for protein expression we use Rosetta which has chloramphenicol resistance Gene which blocks protein production. But since the E.coli is already resistant why we add also chloramphenicol?
What does "copy number of gene" mean? and, for a specific gene, is it possible to check its copy number online, for example from NCBI?
Good job
Thank you sir
Thank you
can you refer any books for this expression vectors to study in detail
You are a natural! :)
thanks alot
Great!!!
You're welcome
can some one explain how the tags like histadine tags is going to be expressed,, isnt our gene already got a stop codon?
The tag sequence can be present before stop codon....
Can i meet you in zoom application to explain me an article?
My doctor told me to present this article but i don't understand this article, i read it many times but my brain not understand anything unforunatlly!
I will pay to you, so please if you can explain it to me💔
thank you so much sir
+Pankaj Pulate glad to hear that
And don't waste too much time changing the stupid colors!