cDNA library

This lecture explains about the cDNA library construction and screening. shomusbiology.w...
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A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which together constitute some portion of the transcriptome of the organism. cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an organism. Similarly, tissue specific cDNA libraries can be produced. In eukaryotic cells the mature mRNA is already spliced, hence the cDNA produced lacks introns and can be readily expressed in a bacterial cell. While information in cDNA libraries is a powerful and useful tool since gene products are easily identified, the libraries lack information about enhancers, introns, and other regulatory elements found in a genomic DNA library.
cDNA molecules can be cloned by using restriction site linkers. Linkers are short, double stranded pieces of DNA (oligodeoxyribonucleotide) about 8 to 12 nucleotide pairs long that include a restriction endonuclease cleavage site e.g. BamHI. Both the cDNA and the linker have blunt ends which can be ligated together using a high concentration of T4 DNA ligase. Then sticky ends are produced in the cDNA molecule by cleaving the cDNA ends (which now have linkers with an incorporated site) with the appropriate endonuclease. A cloning vector (plasmid) is then also cleaved with the appropriate endonuclease. Following "sticky end" ligation of the insert into the vector the resulting recombinant DNA molecule is transferred into E. coli host cell for cloning. Source of the article published in description is Wikipedia. I am sharing their material. Copyright by original content developers of Wikipedia.
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[@shomusbiologyofficial]

All the best

[@architabhattacharya9690]

This is by far the most useful youtube channel I've ever come across..Thank you sir

[@ritika5911]

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[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@gracioustakayidza5204]

Clearly explained in a very interesting way. You make it so easy to grasp the concept. Thank you for the informative video.

[@shomusbiologyofficial]

You're welcome

[@thangadharshinim3054]

Very very thank you for your video sir.its very very helpful. Continue making like videos sir. You are a good teacher. From your video only I have been learning my subjects very easily. I am a big fan of your teaching...

[@nataliepuckett2672]

I only say this to you because I can see your passion behind your teaching for everybodies highest good! I don't see this as everybody's agenda!

[@shomusbiologyofficial]

Thank you

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[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@dr.pratishthasharma6443]

Jus amazing..thanks a lot for making these concepts vry easy to understand

[@user-rq2cp6np4k]

Thank YOU Dr. Suman your videos are very usefull and interesting!

[@gabrieleoliveto2624]

I love your videos, greetings from Italy! I hope you will be able to answer a doubt that I've had for a while now: is there a reason why we don't use PCR to synthesise the restriction sites? (Using a 5' longer end in the primer)

[@piko9311]

Thank you very much for your clear presentation

[@ankitmehra8430]

why blunt ends ligation for large fragment is not efficient?

[@eoncarlyle]

Shomu is the real OG

[@nataliepuckett2672]

I don't know if you'll tell me the truth but does this all mean there are different species of human and if so do the other species suffer because of our human interests? I can't sleep at night learning more and more of what all this means, people suffer because they're hybrid by nature and we pick them apart trying to be like them? Is that what the conclusion of all this wonderful knowledge! I refuse to pursue my profession for suffering of anybody else! We are all different love your species and the hybrids because they all bleed the same! Even if it's different under a microscope!

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are u on drugs lmao

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what did the software you use Suman Bhattacharjee?

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YOu Rock Biyatch!! Helpful stuff!

[@mayukhroy5393]

Sir , your videos are really great.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@simigupta4669]

sir c dna to smjh aa gya pr images clear ni shw hoti h computer screen ki it create a lot of problm ni making notes

[@pratikshamore7520]

Bingo..!! Awesome lecture 😍😘😋😊

[@ravenlhelix]

Is there any chance you would create a channel with your lectures in Hindi/Bengali? Would be really cool!

[@user-zw7en4sq4q]

讲的太棒了，谢谢老师

[@bmbijayanee8399]

Dhanyavad

[@shomusbiologyofficial]

You're welcome

[@simranvohra1974]

Just a bingo lecture🎉🎉🎉

[@diyasekar581]

Thank you sir

[@ganeswarbrahma9211]

samaj nai arahatha kaise kahase paru .thank you sir ....❤❤

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@dhanwants3334]

Thanks so nicely teach

[@rezenehabte434]

well presented

[@shomusbiologyofficial]

You're welcome

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Brilliant!

[@chetanchetan23]

thanks

[@shivasanthu9373]

Very nice vedio

[@shomusbiologyofficial]

Thank you

[@jayajhamnani5600]

Thank you so much
For clearing the concept in a easy way

[@sachinskarennavar9690]

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Entrusting in study

[@jitendraNoida]

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[@pramitlahiri1978]

16:28

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