Hi! At 34:40 you say that the gene of interest is inserted at digestion section, between telomere sequences in circular form. this is not correct. it is digested from there to be linearized but the target gene is inserted at the part that is shown in the figure.
sir, the vector contains it's own DNA sequence too, besides the gene of interest .. so how is the expression of the undesired genes present originally in the vector controlled?
the gene of interest is supplied with a strong promoter which may be activated by an inducer (eg, lactose in case of lac operon), so once the inducer is provided, the gene of interest is expressed, all other genes are not expressed
If my insert is 1092 and l use the pET-28a+ plasmid vector which is 5369 bp long, how long will my recombinant vector be? Assuming l for an example use the EcoR1 RE? thx :)
:) It looks like OSI model communication protocols in IT :D en.wikipedia.org/wiki/OSI_model. IP en.wikipedia.org/wiki/Internet_Protocol or UDP en.wikipedia.org/wiki/User_Datagram_Protocol but in circle :P In communication there are many protocols maybe there some better for this example invented by human not nature :D, it depends how many bits and how You want to sent :)
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HI I BENEFITTED FROM ALL THE SHOMUS BIOLOGY VIDEOS THANKS FOR ALL THE EFFORTS
+Humi Khan thank you. Glad you liked my lectures
Thank you sir.... Very nice informative and excellent lecture sir.. thank you sir... 🙂🙂🙂☺🤗
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nice presentation.
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Hi! At 34:40 you say that the gene of interest is inserted at digestion section, between telomere sequences in circular form. this is not correct. it is digested from there to be linearized but the target gene is inserted at the part that is shown in the figure.
plasmid has a methylase, but how they can be cut it by EcoR1..?
wow......thank you very much Sir !!!
thank you for this video
do you have questions regard to this material to practice before the exam?
Very helpful thank you so much for your efforts
hi thanks
sir, the vector contains it's own DNA sequence too, besides the gene of interest .. so how is the expression of the undesired genes present originally in the vector controlled?
Upls answer this suman
the gene of interest is supplied with a strong promoter which may be activated by an inducer (eg, lactose in case of lac operon), so once the inducer is provided, the gene of interest is expressed, all other genes are not expressed
U r great sir
Long live
Thank you
Good. No lag
Thank you ..thank you so much
You're welcome
lacZ gene in plasmid....can u explain?
will the vector alter the host?
If we use cloning vectors for only for cloning of a gene..... Then how Tetracycline resistant gene get expressed in host cell in case of PBR322?
Is it possible to get the proteins by using cloning vectors?
I think in the presence of the antibiotics. The gene may become active by some activator or inducer. I'm not sure though.
Tks a lot
Refer the book name
suman da plz ar porer videote gene expression vector r cloning vectorer differencesta banow
+Prasanta Paul already there
thank you :)
it is GAATTC..........NOT AGGTTC............u were right at first
why it is necessary to convert mRNA to cDNA for cloning into vector
Nimra Haseeb because we need to make copies of it... Simply inserting the mRNA would be useless
because the dna conatian exon and intron unlikly the mrna just have exon that's why we use the mRNA ton convert it tio cDNA
Super
thnks sir
If my insert is 1092 and l use the pET-28a+ plasmid vector which is 5369 bp long, how long will my recombinant vector be? Assuming l for an example use the EcoR1 RE?
thx :)
thanks to sir
+Pankaj Pulate you're welcome
Thank you :-)
:) It looks like OSI model communication protocols in IT :D
en.wikipedia.org/wiki/OSI_model. IP en.wikipedia.org/wiki/Internet_Protocol
or UDP en.wikipedia.org/wiki/User_Datagram_Protocol but in circle :P
In communication there are many protocols maybe there some better for this example invented by human not nature :D, it depends how many bits and how You want to sent :)
totally bakwaas
isse accha videos you tube pe present hai
Chi itna ghatiya bolte saram nahi aayi Chi Chi... Bhagvan tumhe... Kabhi khus nahi rkhega narendra
bilkul sahi hai yaar...prem...
accha kiya prem.....sachi itna ghatiya reply us narendra ne diya chi....chalo tumhare acche reply se usne apna comment delete kar diya.....that s good....yah public video hai ...kisi ko hak nahi ki aise word ka use kare khas karke study sites par
thank you :)