PBR322 plasmid vector | Cloning vector

PBR322 plasmid vector | Cloning vector - This lecture explains about the cloning vector that is a plasmid vector known as PBR322 vector. pbr322 cloning vector is used for molecular cloning of small fragments of DNA using this specific plasmid vector.
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Thank you for watching the molecular cloning lecture on pbr322 cloning vector for molecular cloning.

[@anujsingh-pm6qw]

Thanks sir very nice...

[@shomusbiologyofficial]

You're welcome

[@Hoxgene]

Can you please elaborate @12:30 on protein synthesis blockage by Tetr gene?? Which proteins are you referring to

[@roshan3190]

Each gene is responsible for a particular protein production in all organisms. So here we insert our gene of own interest into the plasmid (pBR322). That gene can undergo the transcription and translation process and it can produce the protein. In that case if we use the chloramphenicol it restrict the synthesis of protein and the plasmid which carries our gene of own interest can undergo the replication and it won't produce the protein. That's why the copy number can be 1000-3000 per cell. If it produces the protein the copy number is controlled by the rop gene to 40.
I Hope it helps you.

[@saniyashaikh4051]

Nice sir 👍

[@shomusbiologyofficial]

Thank you

[@ronibanerjee7362]

It's actually 4361 bp(earlier 4363)...Please refer to Primrose,gene manipulation

[@lionelprk4088]

❤️

[@shomusbiologyofficial]

Thank you

[@Beautifullife7189]

Transgenic plants ka selection kase kre sir ,

[@Beautifullife7189]

Host cell ,konse use kre ge sir , es plasmid k liye

[@shomusbiologyofficial]

E coli

[@Beautifullife7189]

@@shomusbiologyofficial ok sir thank you

[@sapnaverma2219]

Sir agr hindi vedio aati eski tho jyada acche se smjh aata

[@honeypatil5783]

Hindi mein samj do n sir

[@LonyCa]

first !

[@shomusbiologyofficial]

Thank you

[@abhinavpratapsingh7613]

Don't ask me again anas about this topic..
So, look carefully..

[@mohd_anas40]

Okay Sir👍