What's wrong with making money off these tho? There are many useless videos making money off them, so why don't useful videos like these make money for the hardworking content creators?
this is amazing. i have tried so hard to understand this (i have watched other videos, read an excerpt from a textbook etc.) and now i finally get it. thank you khan academy
WOOOOOOWWWW OMGG THANK YOOOUU I was watching all of my videos from uni and reading all of my lectures...didn't get a thing.....and you are here making it so easy, you literally save me from resit.
Watched this video to understand what the term "DNA Cloning" means and how that's done with the help of recombinant DNA technology. Got to know that it's reproducing of the desirable genes/sections of DNA with the help of certain restriction enzymes, DNA ligase, and the host bacteria that supplies plasmids to attach the target genes and then reproduces them. This video also clarifies the question - "How to engineer microorganisms?" - for which I have seen other videos earlier (insulin production as a case in point)
Thank you so much! I’m in biology D as a freshman right now, and with the whole online learning deal, I’m struggling in the class, and this made the whole idea wayyyy easier to understand! Thanks!
You would not even be doing this level of work as a freshman. It will probably be the first semester of your junior year before you start conducting these kinds of experiments.
The heat shock is happening through CaCl2, which is counteracting the electrostatic repulsion between plasmid DNA the bacterial cellular membrane ( liposaccharide layer), mostly E.Coli as it is most competent bacteriathan for these procedures.
You said we can now make a bunch of copies of that "gene" we want to clone, but we don't really know if that plasmid contained that gene, we only know that it contained the AmpR. As the plasmid could have unsucessfull ligation, so the colonies will still appear as it has the AmpR gene.
How do we know for sure that the bacteria will express the genetic information from the plasmid, for example, even if the colony has taken up the plasmid, why would we be sure that antibiotic resistamce will be expressed?
I was wondering the following, this video tells me that DNA is taken from one organism and put into another organism's plasmid so that that organism can go and create a desired protein. My question is then, does the bacteria know how to splice the introns and exons when it's creating the desired protein. Much more logical to me would be that they take ripe mRNA, (already spliced correctly) reverse transcript that and put it in the plasmid, since bacteria don't have introns and exons.
Because we are using plasmids which carry the factors coding for resistance against some antibiotics and the scientists who perform these experiments are well aware of the plasmids and the resistance they have ..and as the replication occurs in the E coli this gets amplified...
ive struggled so much to understand this topic in my first language lol and now i tried to it in english and your video was so helpful (and pretty) thank yaa
I have a doubt sir. Like u will collect DNA fragment of a virus r plasmid of a higher organism then u will maintain it in such a organism within which u can easily insert a foreign DNa fragment Then which DNA acts as vector here N how many organisms DNA will be involved ..??
This is how insulin is farmed through bacteria. Crazy to think that we are capable of this. Hopefully someone less money-hungry will take this knowledge and overthrow the insulin industry. Fingers crossed
Can someone answer me PLEASE!!! About how do we select the bacteria that took up the plasmid .. what I know that the Plasmid has antibiotic resistance gene NATURALLY (because he said we place it!), so obviously the other bacterias in the plate also have it, so they won’t die ?
the whole idea behind the plating and growth is to allow the bacteria with the resistance to grow and then bacteria that don't have the resistance won't grow colonies. Now that being said, it is science and sometimes the other bacteria will also grow due to naturally gaining the resistance, but that doesn't mean their numbers will be profound.
I just realised.... these vids don’t have ads.... they still aren’t making no money off this.. god bless
yes thanks to not making any money we are successfully homeless .
What's wrong with making money off these tho? There are many useless videos making money off them, so why don't useful videos like these make money for the hardworking content creators?
Yeah, truely one of the best Not for profit organisations out there
@@Ariadne4 He's not saying ads are bad, he's saying no ads are good
Live Wikipedia
Sir, you are my favourite teacher on the Khan Academy platform. Great job !!! You helped me a lot during my studies
Thank you sir
this is amazing. i have tried so hard to understand this (i have watched other videos, read an excerpt from a textbook etc.) and now i finally get it. thank you khan academy
I wish my professor did the same thing to their lectures, would save me time reading textbooks
you should never replace reading the textbook, these lessons are complementary since they are simplified.
True i agree
how is he so smooth with the mouse?
he is using a drawing tablet ! :)
Bruh
That’s what I thought
Ohh, man he is using a writing pad.
Experience *pen
Even 7 years later, he's still the best teacher to ever walk this Earth. Thank you so much for helping me during my studies!
THAT WAS AMAZING!!!
For a moment I imagined you teach us in our university, it would be like a dream 😢
Thank youuuu so much
Even in med school, you're still helping me. Thank you!
You are Doing this stuff in med school?
@@paribhura6704 Lol unfortunately. We just started systems, but we covered this in our basic foundations 1st semester.
@@eniamrahc73 oh! We are doing a brief unit on it but I am in smiths grade, not many details
@@paribhura6704 just have to go back to this stuff again and again…..
Me too lol, first semester of med school, in 2 days I'll have my exam
Great video, Khan Academy never fails to clear up difficult topics/processes.
You can hear the passion he has for teaching science in his videos. Amazing job!
i know right frfrf
That's amazing, to think we can do this inside cells using biochemistry to get what we want! Keep them coming Sal, loving these new (micro)bio vids.
gucci mane is a clone go look at him he is a human so yea do your research XD
This is genetics, not microbiology.
Umm i think to be precise its biotechnology-biomolecular.
I cant believe you could explain biology in the best way! I like biology now..
You just simplified the bulky and complicated material given by my lecturer.. Thank you so much ❤🎉
thank you very much i wasnt understanding it until you explained
WOOOOOOWWWW OMGG THANK YOOOUU I was watching all of my videos from uni and reading all of my lectures...didn't get a thing.....and you are here making it so easy, you literally save me from resit.
Bob Ross of biology :D
started watching khan academy when i was in 9th grade
and hi i'm in my first week of college
turns out no professor teaches better than Khan
:)
Bro SAME! i also started watching since 9th grade and am now in my first year in college
This is the most concise and informative video on this.
I really love khan because learning becomes easier.
Thank you! I was reading my textbook and lecture notes, but still couldn’t understand it. This video explained everything perfectly 👌
Such a great video!! Explained this concept so clearly!
Great video! Thank you, Sir.
May I say that It's pretty amazing how you're fascinated by science 😂 you transferred that to us too!
His videos are life saver!!!
Busy with my project, thank you so much, so easy to follow
Watched this video to understand what the term "DNA Cloning" means and how that's done with the help of recombinant DNA technology.
Got to know that it's reproducing of the desirable genes/sections of DNA with the help of certain restriction enzymes, DNA ligase, and the host bacteria that supplies plasmids to attach the target genes and then reproduces them.
This video also clarifies the question - "How to engineer microorganisms?" - for which I have seen other videos earlier (insulin production as a case in point)
Which biotechnology techniques can be used to proof if certain cell express a particular enzyme?
I wrote Elisa and Western blot.
Pls help?
Who else reads the comments while sal is explaining 😂
You made my reading texbooks life easier
That's the exact way of teaching....loved it
Thank you so much! I’m in biology D as a freshman right now, and with the whole online learning deal, I’m struggling in the class, and this made the whole idea wayyyy easier to understand! Thanks!
You would not even be doing this level of work as a freshman. It will probably be the first semester of your junior year before you start conducting these kinds of experiments.
@@gregsmith6756 whats the point of responding 2 years later? lmao
@@nitro.2405for others whom it may concern 😊
Very well done!! best explanation of this topic
SUPER helpful content - thank you SO much!
Thank you for helping me through my lab courses. I would be lost without your videos.
Thank you for the explantion🌻🌻
Thank u sooooooo much . U saved a lot
You make everything so much easier to understand
Excellent Presentation. Very well done. The colours (against the dark background) make the intended impact.
Got me through my assignment, thanks sir
The first humans lived to be 900 years old now you see why its so important
Pro you are amazing person..really you explain any object i want to study it ..i don’t know how i can thank you 😢❤❤❤
나 한국인인데 쌤 설명 지리네요
Thank you very much your are agood teacher
thanks!!
The heat shock is happening through CaCl2, which is counteracting the electrostatic repulsion between plasmid DNA the bacterial cellular membrane ( liposaccharide layer), mostly E.Coli as it is most competent bacteriathan for these procedures.
Thankyou for the explanation
Thank you Sal. I wish every teacher is as awesome as you are
We love you Sal❤️💪🏾👑
make it easy to understand!! and it is almost identical to my lab materials but in simplify form!! Thank you!
Great vid Sal, you're the man!
Excellent lecturer. Great clarity.
the only good thing about youtube algorithm is that, it introduced me to Khan Academy
Wonderful explanation!
this is good
Thank you very much .finally I got it.
Awesome explanation. Thanks a loooooooooooot.
Thanks you have helped 😊
You are the BEST!!!!!!!!!!!!!
Thank u. 😊
Thank you Khan academy ❤️
와,, 이해하기 쉽게 설명 진짜 잘해주심 ㅠ
Great explanation. Thanks a lot.
excellent tutor!
thank you didnt understand what the overhangs were and overall process of DNA cloning
You said we can now make a bunch of copies of that "gene" we want to clone, but we don't really know if that plasmid contained that gene, we only know that it contained the AmpR. As the plasmid could have unsucessfull ligation, so the colonies will still appear as it has the AmpR gene.
Superb!! Very nice, simple explanation.
Thank you for getting me through my studies! These videos really help piece it altogether.
Much appreciated🙌
Your Northern Irish student😁
Love the Star Wars reference
How do we know for sure that the bacteria will express the genetic information from the plasmid, for example, even if the colony has taken up the plasmid, why would we be sure that antibiotic resistamce will be expressed?
Well explained lecture Sir!!!!!!
You are awesome really, very clear and good video, thank you so much, we will wait for more videos
I was wondering the following, this video tells me that DNA is taken from one organism and put into another organism's plasmid so that that organism can go and create a desired protein. My question is then, does the bacteria know how to splice the introns and exons when it's creating the desired protein. Much more logical to me would be that they take ripe mRNA, (already spliced correctly) reverse transcript that and put it in the plasmid, since bacteria don't have introns and exons.
Isn't bacteria a prokaryote and prokaryotes don't have introns.
awesome video!!
could you make a video and explain different kind if cloning? Gateway, Greengate, Goldenste are some examples I can't differentiate from each other...
What is the solution in the test tube made of?
Tq so much for your kind information
Thanks
The drawing is great!
Thank you thank you thank you
how do we know that the bacterial colony with the desired product only have the antibiotic resistance?
Because we are using plasmids which carry the factors coding for resistance against some antibiotics and the scientists who perform these experiments are well aware of the plasmids and the resistance they have ..and as the replication occurs in the E coli this gets amplified...
Man, thanks I love you.
Homie mentioned the Clone Wars haha. They really know their audience
I am currently making a integrated test and man, you helped me a lot.
Explanation is so good :)
i thought Restriction Enzymes cut the Plasmid at Restriction Sites
Is it me or any one else found that Sir's voice is quite similar to Dr Morton ( the noted anatomist yt channel)
Awesome
I am forever grateful to you because you are the ONLY REASON why I will pass my exam. Thank you thank you thank you!!!
ive struggled so much to understand this topic in my first language lol and now i tried to it in english and your video was so helpful (and pretty) thank yaa
I have a doubt sir.
Like u will collect DNA fragment of a virus r plasmid of a higher organism then u will maintain it in such a organism within which u can easily insert a foreign DNa fragment
Then which DNA acts as vector here
N how many organisms DNA will be involved
..??
谢谢
Thank you so much! 🙂
Thank youuu !!!!
This is how insulin is farmed through bacteria. Crazy to think that we are capable of this. Hopefully someone less money-hungry will take this knowledge and overthrow the insulin industry. Fingers crossed
How do we compensate for the lost DNA fragment that was cut off for cloning?
Can someone answer me PLEASE!!! About how do we select the bacteria that took up the plasmid .. what I know that the Plasmid has antibiotic resistance gene NATURALLY (because he said we place it!), so obviously the other bacterias in the plate also have it, so they won’t die ?
the whole idea behind the plating and growth is to allow the bacteria with the resistance to grow and then bacteria that don't have the resistance won't grow colonies. Now that being said, it is science and sometimes the other bacteria will also grow due to naturally gaining the resistance, but that doesn't mean their numbers will be profound.
brilliant
Thanksss
Thank you so much
You saved my life
hello can u plx tell me from can i get DH5 Alpha e.coli? Do u have any link from where can i order it
what are the products of DNA cloning?
Is there any videos about methods used to obtain DNA sequence?
Best explanation of Recombinant DNA