Polymerase chain reaction (PCR) | Biomolecules | MCAT | Khan Academy
HTML-код
- Опубликовано: 23 авг 2024
- Courses on Khan Academy are always 100% free. Start practicing-and saving your progress-now: www.khanacadem...
Introduction to PCR (polymerase chain reaction).
Watch the next lesson: www.khanacadem...
Missed the previous lesson? www.khanacadem...
MCAT on Khan Academy: Go ahead and practice some passage-based questions!
About Khan Academy: Khan Academy offers practice exercises, instructional videos, and a personalized learning dashboard that empower learners to study at their own pace in and outside of the classroom. We tackle math, science, computer programming, history, art history, economics, and more. Our math missions guide learners from kindergarten to calculus using state-of-the-art, adaptive technology that identifies strengths and learning gaps. We've also partnered with institutions like NASA, The Museum of Modern Art, The California Academy of Sciences, and MIT to offer specialized content.
For free. For everyone. Forever. #YouCanLearnAnything
Subscribe to Khan Academy’s MCAT channel: / @khanacademymcatprep
Subscribe to Khan Academy: www.youtube.co...
![[Special Clip] ATEEZ(에이티즈) 홍중 'Why Do You Love'](http://i.ytimg.com/vi/I6XYXQKN_pY/mqdefault.jpg)
We need a man like this in every molecular biology class, questioning LITERALLY everything. Because EVERYTHING needs a little further explanation Ms. Sawa!! Lol
I LOVE THIS WAY OF EXPLAINING ......GETTING SOMEONE WHO KNOWS AND ONE WHO DOESN'T KNOW THAT WELL
Hi Sal! I am studying pursuing my undergraduate studying molecular biology with a presentation on Real time PCR tomorrow. I really think this is an amazing process especially with respect to the forensic applications! Thanks for this video, it kind of summed up everything I have already covered so I feel really ready! Khan academy has been great since 8th grade and I am still using it now studying for my MCAT so thank you from the bottom of my heart!
I am a layman but I can still understand PCR completely through this awesome video. Thank you Khan Academy!!!
Always love how you guys break down the extent of knowledge to be understood :D
Exactly.
precisely
Omg this is guy is awesome how clear he is in his explanation thank you thank you thank you comme to teach to our community college in Glendale Cali please!!!!
Every video that I see with this man’s voice…. I automatically know that I will learn something ❤. Love it
Best explanation I have seen so far! Yeah Khan Academy!
love this.. have a test this friday and it was hard to understand my textbook
To be clear, you do NOT exclusively have to use Taq polymerase. My lab uses Phusion. What matters is that it is thermostable.
fascinating..I am studying Botany at the university of the free state, this video was really helpfull
i hav understood thanks man
Thank you I have a question What is the purpose of the positive and negative control in the PCR reaction?
shakila rasa the negative control to detect if there is any DNA contaminant in my PCR reaction mixture. After the PCR reaction, the negative control should not produce any band on the gel electrophoresis.
The positive control is the control that contain a piece of DNA that will be replicated using my primers and produce a bands, it is just to check that there is nothing wrong with my PCR reaction steps, denaturation, annealing and extension.
Literally all the questions I had you answered wow thanks.
Thanks so much sir ❤❤
I don’t know if the video mentions it but you can also use pcr to generate multiple copies of a mutated strand by introducing a primer containing a mutant base then favoring only mutant by introducing endonuclease to remove parent strand (mostly like nucleases selecting methylated strands) then repeatly coloniezes bacteria until you got a mutant
Glad and grateful to witness your passion in sharing your knowledge. Bless up!!
I had such a hard time understanding this material until now... thank you!
Helped!
Thank you, this helped a lot!
I had a great time listening and watching 👍
Wow thank you for the lightbulb moment
The polymerase is from Yellowstone and the guy who discovered it took it without permission. When the patent was sold for 300 million, he gave no royalties to the park service
That's tuff
THIS VIDEO SAVED ME
Thank you so much for all the contribution. Much improvement. 😊
Thank you
Thermus aquaticus*
Both thermophilus and thermus are used.
@@crazybull7282 thermus thermophilus is a seperate species
That was really good... Thank you
i'm a newbie for this entire topic. its clear from this description that only a 'small fragment' (if this is the proper term) of DNA is able to be 'amplified' using this method. but for my understanding, what if the goal is to amplify the ENTIRE DNA sequence (say as in 3 billion base pairs of a human genome). can PCR still be successful to do this - if not, why (and what alternative method would be used)? and if yes, is there anything ''different' about the process for an entire sequence? Thanks in advance, for any insights...
soo helpful, Thank you !!
How do you know what exact region you want to make copies of from the original template?
One of the best 👍💯
She sounds like Alexandria Ocasio-Cortez
Great video, thanks
I love this stuff so much WHAT
Can someone explain to why it must be cooled afterwards separating the DNA? And then reheated for that last step?
Why all the great teachers on RUclips .Why do we need to pay for college by providing us with such an average teaching?
Great video - thanks Sal!!
Excellent, thank you
wow amazing!!! thank you
We use Pcr at out Food Micro Lab..to make sure food is safe for public
How are the nucleotides added and in what form?
*Important video*
how do you select the right primer when you want to detect a new virus (in case of the first ever testing for that supposedly new virus) ? How do we know about the DNA- when we are trying to figure out if this is a new unknown virus?
Primers can be designed only if the sequence is known. So for detecting a new virus, the first step would be to sequence a sample of it isolated from a patient (using techniques like sanger sequencing) and confirm that it is a new strain of virus. Then you can design the primers by using a sequence that is unique to that strain. Now, you can test if this virus is present in the population by PCR. Only if that unique sequence is present in the patient's sample, will the PCR work and amplify DNA. Hope this helps.
I have a question, why don't we just put the separate enzyme in instead of heating it up?
Can u plz subtitle ur videos, as you have done on google.
Can I find primers on black market?
Why do we need to cool it down for the nucleotides to bind with the DNA?
I think Taq polymerase and primer function at a cooler temperature.
Wouldn't it have something to do with proteins denaturing under high temperatures? You would have to cool them down first
hey y'all. just curious but what's the difference between this channel and khanacademymedicine?
This for those studying for the MCAT to get into med school, and the other is for those in med school studying for their classes. Just technicality but yeah some of the mcat videos dont cover things beyond the scope of the mcat
Exactly PCR
awesome
I Don't Just love these videos as they help with Mathematics but the person who does the videos sounds alot like the quite popular PokeMon RUclips +TheMunchingOrange .
This isn't the best explanation of PCR, but it helps summarize.
Can a primer which has been involved in one cycle be utilised again in another cycle? Or is it necessary that a fresh primer is necessary?
the primer becomes part of the new complementary sequence, so it's not "free", as it were, to go float off and bind to some other DNA strand. in that sense, it has no choice; once it binds it is fixed there. as the cycles go on, eventually the amount of primer (as well as dNTPs) get depleted.
How come you have an MCAT tutor that hasn't made PCR before?
Same way I can teach you how to do CPR without actually ever having to perform it on live person.
I did PCR in my second year of college. In four years I’ve done it over 5 times.
new-KLEE-yo-tides
or
new-CLAY-yo-tides?
Why are primers made for both sense AND antisense DNA targets.
Greg Fox to replicate both of the DNA strand, sense and antisense. If you design primers for only the DNA sense strand for example, it will replicate only the DNA sense strand making the number of copies of this strand higher than the DNA antisense strand
Thanks, I came to realize that a few days after :)
its to make 2 copies and not just one , therefore making it super efficient!!
can't we clone the dna fragment in a suitable host using vector??...
You can. Transfect bacteria with DNA and the bacteria itself will replicate the DNA
yasss bio
Omg First
Thermos Aquaticus found in hot tubes in Yellowstone
He pronounces nucleotides wrong. :/
how does RT PCR work?
It's the same just add prime and reverse transcriptase to your RNA you will get one DNA strand and then use the same steps in the video...
♡
Who is the professor here guy or the girl😂
My dad says that this is actually the correct way to pronounce "nucleotides". And I think he knows what he is talking about because he has a BA in Genetics
:)
I'm cringing at how he pronounces "nucleotides."
Yeah keep focusing on the unintelligent aspects. Low IQ
i ship yall
Info is good, but terribly drawn... The difference between the shortened and original strand is almost non-existing. Make it shorter, use another color or something along those lines.
Seek Christ Jesus YHVH
FORTNITE OR FAIL TEST
Her voice and use of words is really annoying (as she would talk to kids), exAAAActly...