Andrey, you have a real gift for teaching. I was glad to donate to your site this evening, as AK Lectures is one of the absolute best sources for instructional science content on the web. Thanks for making us all MUCH more knowledgeable!
+Daniel Holley Hey Daniel, thanks for your donation, I greatly appreciate that! Thanks for all the kind words, happy that my content is helping! Have a great one!
I've been using your lectures throughout my undergrad, and now I'm in 1st year of 'Biochemistry & Molecular Genetic' PhD program. Yet, I still find your lectures very helpful. Thank you so much! 🙏
ak u are the best teacher i have ever learned from. Now i have a hope that i would get very good marks in my boards this year. If that happnes i wauld never forget u in my whole life, i hope you keep on making us so knowledgeable, thank u
thank you very much for these lectures. every day i watch these videos. i am a biochemistry student. and these videos help me a lot. again 1000 thanks.
Honestly, if it weren't for you I don't know how I would pass my classes. Thank you for your organized lectures. You are literally the best on youtube!
@AK LECTURES can you please tell us, Just roughly,What is the quantity of dNTPS and Primers required per cycle? Because the no of strand keeps getting amplified after every cycle,so that means more dNTP's,polymerase and Primers required. I will be really grateful if you reply.
Picked this video because I just KNOW Andrey will deliver and I WILL understand by the end of the video. Huge props to this channel for being the best at explaining hard biology concepts!
YOU ARE AMAZING. You are saving my life in biochemistry, physics, and molecular biology. No one has ever explained anything better than you. Thank you!
Don't know what else to say, i found PCR complicated before---you could not have made it any easier to comprehend----What temperatures do regular Polymerase operate about 37-40 degrees?---why do we need the heat resistant if step 1 and 2 has been achieved is it to minimize annealing issues??
Thank you so much!! You are the only one on RUclips to do that kind of awesome videos of avanced biology!! I speak french and i understand everything you said, thanks a lot!!
Honestly, it's because of you I understand my genetics class. After reviewing my slides and listening to your videos it puts everything into perspective and understanding
You truly are gifted sir. I’m currently a PGY 1 surgery candidate and I’m having to do an entry basic medical science exam. Straight to the point. Short & clear. Big 👍🏾 from 🇵🇬.
But I dont understand how the primers are created... how you create those specific chains? Its hard for me to visualize how the nucleotides are created to be all same, or how you create a radioactive nucleotide or how you dye them
GREAT VIDEO AND INSTRUCTIONAL PRESENTATION. This written and oral demonstration is good up to a point but would it be possible for you to do an ANIMATION so we can VISUALLY see exactly how this PCR process unfolds. Also would you be able to do a video explaining how this PCR technology is used for practical purposes and circumstances for instance,to identify the guilty suspect in a CRIME scene and secondly, how it would used to DETECT MINIMAL RESIDUAL DISEASE in LEUKEMIA for example. Thanks.
Andrey K Thank you very much for your wonderful and very useful way of explaining the information. I always use your style of explaining information to my students
One question , 72 degrees is the ideal temperature for the DNA polymerase so initially while increasing it to 94 degrees at 74 degrees do we observe change? like in the first step because the temperature will definitely be at 74 for a few seconds at least . Will the polymerase get acivated maybe?
thanks you have just explaned what it took my lecturer 5 hours to explain . love that you take time to reapeat concepts and I am thankful for the drownings helps a lot with remembering. and of course you are a grate teacher
thank you sooo much for all your videos please l want to understsnd relative and absolute quantitation,efficiency,melting curve analysis, multiplex please explan 😢
after the reverse transcription, we get a single-strand cDNA. I'm quite confused about how to get double-strand cDNA by PCR, cause in the experiment we use BD smart RACE cDNA amplification kit to do this, but I don't konw the function of NUP Looking forward to you reply! Thank you so much
I never donate to anybody, but you are an absolute genius to explain such complicated concepts and make them so simple to understand. I always look for your videos. I don't have much money to give as a student but i will gladly give you $10. Hope it is worth your while.
thanks for the video. I can ask a question, why in a PCR it is highly recommended to perform a premix instead of preparing the reaction tubes independently?
HOW DOES THIS GUY KNOW EVERYTHING IN LIFE?!
SUCH a job well done.
lmao
🎯
😅 He Is Really a Great Teacher
he's a physician
Andrey, you have a real gift for teaching. I was glad to donate to your site this evening, as AK Lectures is one of the absolute best sources for instructional science content on the web. Thanks for making us all MUCH more knowledgeable!
+Daniel Holley Hey Daniel, thanks for your donation, I greatly appreciate that! Thanks for all the kind words, happy that my content is helping! Have a great one!
I agree with you. I looked at other videos but AK lectures are the best. #Cheers
3 years later,im here to appreciate this channel. AK lectures is really the best source for science. thank you so much.
You are so precise and concise. It is truly a pleasure to listen to you lecture.
+ikaties1 thank you! appreciate that
One little mistake on 2:02
You say that primer is "a relatively short sequence of DNA" but primer is a sequence of RNA
@@jonahinthewhale he's talking about DNA primers
I've been using your lectures throughout my undergrad, and now I'm in 1st year of 'Biochemistry & Molecular Genetic' PhD program. Yet, I still find your lectures very helpful. Thank you so much! 🙏
Herbs are the best, I got cured completely using DR RORPOPOR HERBAL on RUclips herbs supplements to defeat PCR • his medication is active.......
Best teacher on RUclips
ak u are the best teacher i have ever learned from. Now i have a hope that i would get very good marks in my boards this year. If that happnes i wauld never forget u in my whole life, i hope you keep on making us so knowledgeable, thank u
cbse ?
yes !
Just thanks for making the biochemistry as simple as that
thank you very much for these lectures. every day i watch these videos. i am a biochemistry student. and these videos help me a lot. again 1000 thanks.
I am also like that, because biochemistry Examination in 30 marks, I have given by AK lectures
Thank you, for 1000000 million times.
Honestly, if it weren't for you I don't know how I would pass my classes. Thank you for your organized lectures. You are literally the best on youtube!
OMG! I'm prepping for USMLE and your lectures are awesome. Keep it up!
I'm completely in love with your channel! Thanks for making this videos and for your incredible explanations. I will definitely donate. Cheers! 🙌🏼
I have no words. absolutely amazing. Thank you. God bless u.
I really like this lectures. It is very helpful for me to review the basic knowledge. Thanks a lot!!
My trading journey was a matriculation of highs and lows, literally just like the market. you up, you down. Now I'm constantly up.+
Over the past 5 years, buying Bitcoin every week performed better than timing the market 86% of the time.
@AK LECTURES can you please tell us,
Just roughly,What is the quantity of dNTPS and Primers required per cycle?
Because the no of strand keeps getting amplified after every cycle,so that means more dNTP's,polymerase and Primers required.
I will be really grateful if you reply.
Picked this video because I just KNOW Andrey will deliver and I WILL understand by the end of the video. Huge props to this channel for being the best at explaining hard biology concepts!
YOU ARE AMAZING. You are saving my life in biochemistry, physics, and molecular biology. No one has ever explained anything better than you. Thank you!
Thanks, great video
Don't know what else to say, i found PCR complicated before---you could not have made it any easier to comprehend----What temperatures do regular Polymerase operate about 37-40 degrees?---why do we need the heat resistant if step 1 and 2 has been achieved is it to minimize annealing issues??
I finally understood PCR.. yay!
you have really nice teaching skills.
Much love from Kenya 🇰🇪🇰🇪🇰🇪🇰🇪
Am a biochemistry student and your videos have been indeed helpful. Tanks a lot
Absolutely invaluable lecture in understanding PCR. Thank you so much! xoxo
Now, this is something that needs to be truly appreciated.
Well done Andrey!
why am I paying college to watch your video
On the second DNA diagram it runs from 3’ to 6’, is that a mistake? Or am I missing something?
Thank you so much!! You are the only one on RUclips to do that kind of awesome videos of avanced biology!! I speak french and i understand everything you said, thanks a lot!!
superb teaching sir it helped me a lot in my examinations i think this the best site i have ever seen
Honestly, it's because of you I understand my genetics class. After reviewing my slides and listening to your videos it puts everything into perspective and understanding
اجعل لك وردا يوميا من الصلاه على الحبيب المصطفى صلى الله عليه وسلم تفوز في الدنيا والاخره.
Just awesome.
Primer length could be anywhere from 8 to 22 base pairs.
You truly are gifted sir. I’m currently a PGY 1 surgery candidate and I’m having to do an entry basic medical science exam. Straight to the point. Short & clear. Big 👍🏾 from 🇵🇬.
So how does this fit into Infectious Disease Detection?
It doesn’t…
They are calling our own genetic material viruses and calling viruses our own genetic material.
By use with a prognosis and other diagnosis techniques. The detail is in the patents, I have one example on my channel.
Thank you , you've always made my life in university easier
Amazing 1 no. Bhau..
YOUR LECTURES ARE AWESOME , ITS HELPING ME TO PREPARE FOR AIPMT ( All India pre medical test) VERY EFFECIENTLY !
u make me curious n excited to know more,,, THANK U 💔
thank you for all your lecture videos, it is super helpful!
this is a great video. you're really saving my life the day before exams!
These videos are awesome, you make complicated concepts easy as pie to understand!
why dNTP, not just the DNA nucleotides? I mean sing phosphate.
But I dont understand how the primers are created... how you create those specific chains?
Its hard for me to visualize how the nucleotides are created to be all same, or how you create a radioactive nucleotide or how you dye them
GREAT VIDEO AND INSTRUCTIONAL PRESENTATION.
This written and oral demonstration is good up to a point but would it be possible for you to do an ANIMATION so we can VISUALLY see exactly how this PCR process unfolds.
Also would you be able to do a video explaining how this PCR technology is used for practical purposes and circumstances for instance,to identify the guilty suspect in a CRIME scene and secondly, how it would used to DETECT MINIMAL RESIDUAL DISEASE in LEUKEMIA for example.
Thanks.
Excellent work👍👍👍👍👍👏👏👏👏😘👏👏👏👏
finallyyyy im so happy i finally understood PCR thank you so much .....u do awesome videos ^^
At last you said that PCR uses bacterial cell. so, doesn't it takes place in a beaker or a petradisk. i mean where is the use of bacterial cell here?
thank you sooooooo much.
Boy, I wish I'd had had this fellow for Biochem AND Organic Chem. Wow.
Andrey K
Thank you very much for your wonderful and very useful way of explaining the information. I always use your style of explaining information to my students
Thank you so much for making videoes that are very much understable.
What's the purpose of amplification tho? Why can't we just study the target gene?
The only thing I want to amplify here is the volume of this video, man I can't hear anything......
Why does it feel like I am watching Ben Shapiro? It feels like you are about to reck libtards every second of your video.
This is exactly what I was looking for. Thank God I found you.
can you please provide the resources used for this video.
I like your presentation style although I didn't understand what you are talking lol
Thank you, you made this so much simpler than my lecturer
kofi gansah you're welcome! :)
COVID-19 Diagnostic testing FTW!
can the dNTPs not get exhausted during the PCR Process sir?
Sir could you please make one lecture on primer designing or on its features????
what is meant by correct pcr? this is in the second cycle of pcr.. long product?
i enjoy while watching ur videos your way of teaching is so cool it's not boring at all ...
back again in 2023 i need this lecture after 5 years thank you
This is truly awesome. I watch this for my final exam.
Thanks
Guys do you know why sometimes we use temp of 95 C or 98 in denaturation?
Please Upload A Lecture on Types Of PcR
The lesson make me to be bright in PCR technique.
amazing! clearly understood..thank you!
what about the Buffer system to stabilize the pH?
I am going to graduate because of you just want you to know.
I got the idea by simple explanation,good job.
who are the 41 people that would actually dislike this ?
Excellent lecture Andrey, thank you very much
you are best best. Sorry i don´t have better words to describe you with
Page 215
Le soleil = sun
Sur = on
Sous = under
Si = balaa or na_m (Ar)
I am learning so much from you! Thanks!
Such an amazing resource!
What are your thoughts on DNA transduction as evidence by empirical results done by Luc Montagnier?
how, if you can separate the 2 parts of the helix, you can use either one of the other ? how you select them?
One question , 72 degrees is the ideal temperature for the DNA polymerase so initially while increasing it to 94 degrees at 74 degrees do we observe change? like in the first step because the temperature will definitely be at 74 for a few seconds at least . Will the polymerase get acivated maybe?
how come you're so smart? you're intelligence is amazing! unbelievable!
THnx alot for all those great lectures
can you tell me why only DNA POLYMERASE 1 is used in pcr not 3
thanks you have just explaned what it took my lecturer 5 hours to explain . love that you take time to reapeat concepts and I am thankful for the drownings helps a lot with remembering. and of course you are a grate teacher
.....i think I love you! seriously just understood 3 weeks of microbial genetics lab/lecture from the last few videos. God bless you
thank you sooo much for all your videos
please l want to understsnd relative and absolute quantitation,efficiency,melting curve analysis, multiplex
please explan 😢
after the reverse transcription, we get a single-strand cDNA. I'm quite confused about how to get double-strand cDNA by PCR, cause in the experiment we use BD smart RACE cDNA amplification kit to do this, but I don't konw the function of NUP
Looking forward to you reply! Thank you so much
I am currently obtaining my DNA Fingerprinting certification. This is a review for me. However I must say you have a gift for teaching
I was just wondering - in my textbook, it says that the temperature is 68C for annealing rather than 54C. Do you know why this is?
At 12:35, what about the primers? Wouldn't you need to add more for further cycles?
Great explanation. Thank you so much. You clearly know your stuff and are able to communicate effectively.
Your videos are so detailed. You are a good teacher. Your videos have been so helpful. Thank you so much
I never donate to anybody, but you are an absolute genius to explain such complicated concepts and make them so simple to understand. I always look for your videos. I don't have much money to give as a student but i will gladly give you $10. Hope it is worth your while.
This was absolutely amazing!
you make biochemistry tolerable please never stop making videos
thanks for the video. I can ask a question, why in a PCR it is highly recommended to perform a premix instead of preparing the reaction tubes independently?
Thanks. Really broke it down into tiny understandable pieces.