qPCR (real-time PCR) protocol explained
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- Опубликовано: 28 июн 2024
- Hey Friends,
qPCR is frequently used in many labs around the world. It makes up for the major part of the testing strategy against Covid-19 right now and is applied whenever one has to determine gene expression analysis. Real-time PCR works with fluorescent reporters so that the reaction progression is visualized after each cycle.
Cheers
Henrik
Time Codes:
0:00 quantitative PCR
2:15 SYBR Green qPCR
2:42 TaqMan Probe qPCR
3:31 qPCR results
4:14 Applications 
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This is by far the best qPCR video I've seen and I have been searching for a solid 3-4 hours.
You're my hero, thank you.
i've not watched an explanation simpler, clearer and detailed than this, thanks buddy!
I love how short and to the point your videos are. Great job!
This video absolutely made me understand within 4 min, thanks a lot for giving the light to the novel one
Bro you just saved me from pulling an all nighter for my molecular biology homework thx a lot
Hahaha
That was very well explained. I had a hard time understanding this mechanism in other videos, but yours just made it so easy. Thank you for sharing!!
Awesome!
Excellent summary on the mechanism of Taq Man's work. Cleared the whole concept for me there!
Love the way you explain things! So clear and easy to understand!
I love videos that explain a video well and in a few minutes time, thank you!
Thanks for this! Super easy to understand. I'm using qPCR next week for my undergrad research.
I love the depth of your explaination
Thank you for that short and well-explained video!
Oh god that was quick and effective. Unbelievable. Thank you
Thanks Henrik, brilliant video
This video made me understand what is qPCR. thanks for making an easy video that can be viewed by science students. more blessing
Thank you sooooo much! This was amazing! You are brilliant at explaining!
Great video, so clear. Thank you!
Thank you so much, Perfectly summarized. You earned yourself a subscriber😊.
Excellent summary, thank you!
just found the perfect explanation! thank you very much from Spain :)
Perfect explanation, thank you so much
i was reading the wiki article about this one just now, what a coincidence!
thank you!
Fantastic video, thank you!
Your content is so touching
OMGG, thank you so much, explained very nicely & in a simple fashion 👍🏻
Excellent-Thank you!
Very informative, as always :)
so touching for an excellent video
Thanks a lot for your video, very simple but very clear
this is great explanation. very clear and succinct
Great overall presentation
reallygreat video again. Me and my friend are having such an easy time understanding this content.
Perfectly explained
Excellent video!
This video helped me a lot!
Very good explanation with all necessary points and concepts. Thankyou so much 😊
Thank you so Much Sir for your amazing explanation .
this explanation is clear and really helpfull. Thank you so much. love this channel
thank you for this presentation
Helpful! Thank you!
This was clear and very helpful thank you!
Hi. Nice to meet you. This is Walker from Hangzhou Bioer Technology Company Limited. We are IVD and PCR products professional manufacturer.
Great video!!! The pronunciation, the video, and the content, all of these are very valuable! Could you please produce a video on how does loop-mediated isothermal amplification works? Thanks in advance! you are such a great Toutuber!
Very specific, I will put it one the list and see whether it fits some day :)
Amazing informative video
Thank you, for the wonderful explanation! I liked and subscribed to you definitely!!
Very well explained
Hervorragend! vielen Dank
Love the German accent and your explanations, keep going!
Thank you so much
Thank you so much!
amazing video
Amazing 1....
Thank you:)
Thanks a lot sir ✨🎊
Thank you ❤
thank you
LOVE YOU!
Very useful, thanks!
On my wishlist for a future video: dPCR (and why ddPCR is a specific form of it).
Never heard of that, but great idea! Thanks!
@@henrikslab It means digital PCR. The Wikipedia article isn't bad at all, but I think it would make a nice video of an exciting new technique!
Thanks!
Thank you so much! That means a lot to me!
Is the sequence of the primers ( for a specific gene) used in real time PCR different from that sequence used in the traditional PCR???
In one point I would say yes! mRNA usually contains exons but not introns.. so the primer for a specific gene should be a sequence of an exon or even span an exon-exon junction (exon-spanning primer).
Why not using exon-intron primers? Because that enhances the risk that you amplify genomic DNA, but you want solely detect mRNA of that sequence (to see whether the gene is expressed)
wow
Is there any difference between RT pcr and taqman pcr?
In class: teacher explains this method for over 2 hrs and I'm note sure if I understand it.
Random guy on the internet: "Let me explain it to you in under 5 minutes"
Some teachers could really learn how to teach things from videos like this...
What is DNA Concentration minimum for use qpcr
I love you.
W
Could you do a video on the advantage and disadvantages of each qPCR assay?
Honestly: not planned!
danke mein deutscher Bruder
So in PCR there is no fluorescent dye ? Only in qpcr ? Also when do when use syber green and when taqman ?
For PCR you also use DNA staining Dyes (most are fluorescent)... But you just use the dye to visualize the band on the gel.
It depends when to use which one, but it is most likely a choice done by the lab (both work well)
@@henrikslab thank you for replaying.
So the results of PCR is not measured in a computer algorithmically ?
@@ysxydx3763 Usually not, no! PCR does not quantify DNA material, but qPCR does.
@@henrikslab I don’t know why it’s difficult for me to understand this whole process. Say you have a dna sample that you are not sure if it has a virus or not , you take that sample and run it through PCR , now it’s multiplied then what happens ? How can I detect the viral dna ?
@@ysxydx3763 The key to detect that viral DNA is the primer! This primer, with a sequence of very specific ~20 nucleotides is designed to ONLY bind to the DNA of that specific virus... without the DNA sequence of that virus, the primer can not bind and nothing is replicated. But in presence of that viral DNA, the primer binds and replication can be initiated!
Is one able to determine the titer or their virus sample through using qPCR and if so how?
Using a standard curve and the linear equation, x = your measured qRTPCR CT value
, get your standard curve plug in the numbers to solve for y, then power for whatever log you used in the sample.
Then you can get whatever virion concentration was in the sample, which is what I assume you're asking for.
Hello Henrik can you please send this PPT.
Are you German?
Yes
No I’m not germane
my brain is exploding
did it stop
can this detect marijuana or thc ? And if so for how long back can it detect it
Bruder mach mal auf deutsch bitte
You can cut up many spaghetti and then multiply them unknown. Cheeky rip off nonsense.
yes throw the spaghetti in the thermal cycler. that' s what it' s for
Sounds like a german accent
Great video!
Thanks!
Thank you!!
You are welcome :D It's not much but your video really helped me to understand qPCR better!
👍👍👍
Hi. This is Walker from Hangzhou Bioer technology company limited. We are professional PCR products manufacturer that makes DNA/RNA purification machine, DNA/RNA purification kits, thermal cycler, PCR mobile laboratory, DNA/RNA diagnostic PCR machine , antigen/antibody covid virus kit and DNA/RNA detection kits.
@@walkerchen7336 your ID says your first name is Mark and you're somehow Walker? LMFAO