I have been watching a lectures for a few weeks now, i realize that he is actually a but of a genius. Maybe we don't recognize all of them in society and some of them could be teachers like him, but he seems to be a person with multipotentiality. Intelligence, clarity, diverse subjects, hats off to this great man!
you are a legend!!!!!!!! I got an exam tomorrow and wasn't too sure of the reason cDNA was created and how it was created, keep up the great work!!!! You will achieve great heights my friend!!!
Most understandable lectures that I have found in my life, so clear explanation, I go through most of the times with the lectures what i need in my exams,, best wishes for him always
I am finding myself SMILING AT DNA TECHNOLOGY because you are explaining it so beautifully, reiterating things so we mortals can keep up and tying concepts together from film to film. Awesome. You are the go-to channel for my UK A level science teaching prep. Just made a bit of donation.
Awesome video! Thank you for making it. This helped me tremendously to prepare for my national exam. I'm not the type that can just read about a process and grasp it; I have to see it or perform it to truly understand it. Thanks again!
Damn, it was all that easy. That guy from 8 yrs ago, just destroyed my instructors 1 term period teaching skills in 12 minutes. Respect man thanks a lot.
Excellent teaching skills!!! You make molecular biology to look very simple and understandable. Great job which arises from a good knowledge. Thank you.
Your video has a mistake. You said that for the eukaryotic mRNA, the exons must be spliced out but it's the introns that are spliced out during the post-transcriptional modification to produce the processed mRNA. time 2:30
That's not a hard-fast rule. mRNA can undergo "Alternative Splicing" where exons are either spliced out or left in the mRNA. It is based on the splice site that the spliceosome decides to function at.
+QuentinWolffMusic no it won't be a problem because the translation of the mRNA formed from this cDNA would have a start and stop codon flanking the gene of interest, so any no. of dNTPs before the start codon won't alter the final protein. I hope ths,helped
You have some excellent lectures! Could you do one on YAC's and BAC's. If you have already I haven't been able to locate it. Thanks for you teaching methods!
No, DNA is not single strand. What we do first is to create the ssDNA complementary to the ssRNA. We now have to destroy the ssRNA to build the complementary in DNA. That is why we use high pH to eliminate RNA and only obtain ssDNA. Then with DNA polymerase the second strand of DNA is created. Now we have dsDNA. Nothing of this means that DNA is simgle stranded, only when we need it to be in the process using heat.
Why do we need to synthesise poly c tail dna primer?? We can use poly a tail dna primer it is complementary to the cDNA.. And we don't need to add terminal transferase
How can we know that the poly T tail won't bind to the poly A tail of another mRNA molecule rather than the mRNA molecule of interest since all the mRNA molecules have a poly A tail
You don't. Poly T-tails will be used for non-biased cDNA library generation. If you want to make gene specific cDNA only, you will need a 3' reverse primer that is sequence specific.
Maybe already have long time this video but I have a question, and I hope someone can answer me. There's no technology to create the protein using our eucaryotic mRNA and to avoid the other steps to get a protein (eucaryotic)? I mean, if we can extract the mature transcript (modified mRNA -3' poli A tail, 5' cap and spliced exons) from a eucaryotic cell, why we need to form, make or any word you want to use, cDNA and then use procaryotic cell?, unless there's no technology to use directly the mature mRNA. Thanks
because retroviruses can insert specific DNA fragments into the host original DNA, this means everytime the host cell reproduces it will have this new modified DNA which can be translated in the new RNA , if you directly insert mRNA it will only be used once because mrna degrades over time
since the revierse transcirbatse need a primier to start doing its jop whats if we inserted a normal dna polymerase instead of the RT wouldnt it do excatly the same process of making complementry dna to the mrna ?
I don't think DNA polymerase will do its job on a single-stranded mRNA, as it cannot use the mRNA strand as some sort of 'template strand' (mRNA strands contain uracil instead of thymine). So that is why reverse transcriptase is used
Hi. I want to sintetize de Oligo dt(12-18) and Random Primers (d6)n and save some money. How do I have to configure de requirement (order) for the primer syntesis lab? I know that the 5' of the oligo dt must be modified wiht a PO3, but that's all I know lol... Please, help me
Why couldn't we just directly isolate a DNA sequence that will produce the required mRNA? Instead of going from mRNA to cDNA to go back to mRNA that is needed?
This man is an absolute saint -- never seen anyone else be able to explain such complex topics so eloquently -- a godsend for MCAT studying!
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
I have been watching a lectures for a few weeks now, i realize that he is actually a but of a genius. Maybe we don't recognize all of them in society and some of them could be teachers like him, but he seems to be a person with multipotentiality. Intelligence, clarity, diverse subjects, hats off to this great man!
No "um" "uh"s man. Flows soooo nicely! Thank you!
he's very professional
The best lecture I've seen on this topic. Whenever I forget certain basics, I rewatch this tutorial to refresh my knowledge.
you are a legend!!!!!!!! I got an exam tomorrow and wasn't too sure of the reason cDNA was created and how it was created, keep up the great work!!!! You will achieve great heights my friend!!!
Most understandable lectures that I have found in my life, so clear explanation, I go through most of the times with the lectures what i need in my exams,, best wishes for him always
You sir are an excellent teacher.
I am finding myself SMILING AT DNA TECHNOLOGY because you are explaining it so beautifully, reiterating things so we mortals can keep up and tying concepts together from film to film. Awesome. You are the go-to channel for my UK A level science teaching prep. Just made a bit of donation.
I swear this man is the savior of my GPA
You are one of RUclips's best. Your work is excellent.
My already tremendous respect has increased ten fold for you after this video
I'm not the first one who says this: You are awesome! I admire your career, thank you for doing this!
This guy killed it. Amazing talent in presentation and clarity of information. Thank you so much.
Awesome video! Thank you for making it. This helped me tremendously to prepare for my national exam. I'm not the type that can just read about a process and grasp it; I have to see it or perform it to truly understand it. Thanks again!
Damn, it was all that easy. That guy from 8 yrs ago, just destroyed my instructors 1 term period teaching skills in 12 minutes. Respect man thanks a lot.
Damn it was really 8 years ago?
@@AKLECTURES time is passing mister, you did absolutely well. We are-mbg students- still watching your videos. Thank you
Best video EVER!!! You are dedicated to teaching this concept and it shows, I wish you great wealth from your efforts.
the greates mentor i ever seen ...hope you continue making those videos
6:08 A poly-C tail should be a poly-T tail.
explain such a complex set of procedures in a very clear logic. thanks for saving my life in the bio exam :)
Best teacher I ever had
you are an amazing teacher...thank you for explaining everything in such a clear and easy manner.
Excellent teaching skills!!! You make molecular biology to look very simple and understandable. Great job which arises from a good knowledge. Thank you.
Excellent indeed, excellent in spreading wrong information.
Thank you for all your videos.
you have a big SHalom from Israel. :)
Its really a continuous and clear effort in this filed, I do appreciate your hard work and best, simple explanations. Many thanks
thanks for your assistance which made my biology life easier as your lectures are easy to understand and straight forward thank you!!!
I love this channel. You've pulled me through so many courses. Thank you
Fantastic Overview of this process.
Damian Clark thank Damian! :)
AK LECTURES
Tools of biotechnology
Thank you thank you thank you! I've been looking for a bit information all over the web and this video contained it. Great lecture. Subscribed.
Man, you will go to heaven!
a very usefull subscription after a long time...
Your video has a mistake. You said that for the eukaryotic mRNA, the exons must be spliced out but it's the introns that are spliced out during the post-transcriptional modification to produce the processed mRNA. time 2:30
That's not a hard-fast rule. mRNA can undergo "Alternative Splicing" where exons are either spliced out or left in the mRNA. It is based on the splice site that the spliceosome decides to function at.
I just find this channel, it's amazing!
But I have a question: The gene is different at the end, there is a new codon (CCC/GGG) which can produce another protein, isn't it a problem ?
+QuentinWolffMusic no it won't be a problem because the translation of the mRNA formed from this cDNA would have a start and stop codon flanking the gene of interest, so any no. of dNTPs before the start codon won't alter the final protein. I hope ths,helped
Beautiful illustration AK
Amazing! Thank you for doing lectures in youtube!
You have some excellent lectures! Could you do one on YAC's and BAC's. If you have already I haven't been able to locate it. Thanks for you teaching methods!
Thanks for the video, it is very clear and easy to understand, help me a lot.
sir u r the best....ur teaching method is amazing...so easy to understand
Great job explaining this! couldn't be any clearer!
No, DNA is not single strand. What we do first is to create the ssDNA complementary to the ssRNA. We now have to destroy the ssRNA to build the complementary in DNA. That is why we use high pH to eliminate RNA and only obtain ssDNA. Then with DNA polymerase the second strand of DNA is created. Now we have dsDNA. Nothing of this means that DNA is simgle stranded, only when we need it to be in the process using heat.
Thank you for existing Sir, this helped a lot
There is an error at 2:25 ( you said "exons that must be spliced out", when it is Introns )
You are all rounder
Great sir dashing teaching methodology sir god bless u
Thank you, sir... You are a life saver!
So clear and succinct. THANK YOU! BLESS YOU!!!
You are a medical hero! Thanks a lot!
Hii ! I'm from India...love your lecture...make more videos on life science.
really excellent teaching. Thank you very much
amazing video, perfectly explained
DNA polymerase isn't needed to finish synthesizing the ds-cDNA molecule. RTase has DNA polymerase activity.
Silly question, do humans have reverse transcriptase or is that only found in certain viruses
Thank you Sir ! You open my mind.
this is amazing and simple to understand ! thank you !
thank you so much. with your clear explanation i start to love molecular biology. ;)
exons that must be spliced out? I thought introns, as you said in the beginning? :/
he just said it the other way. but he puts the concepts so clear that no one notices it
Fantastic video!
So helpful video, thank you so much
Very nc lecture sir😊😊😊...thank u
everything is just perfect ... awesomeee
Only partially digest RNA with RNAse H, not the entire RNA
Why do we need to synthesise poly c tail dna primer??
We can use poly a tail dna primer it is complementary to the cDNA.. And we don't need to add terminal transferase
Very easy to understand!
how are sticky ends attached to the two newly generated cDNA?
in step number 6 the 5prime end consists a hydroxyl group,do a 5prime end consist hydroxyl group or phosphate group?
Excellent video!
Nahu Dimitri Thanks! :)
Thanks, sir. You are awesome.
Thanks sir.......you are genius
Page 43
4.7.'76
3.7.'76
From : Adams, J.
To : Adams, A.
9.4.'22 = 7.9.'43
We bought Ak_k in P. C. for our 1st son.
Great video, thank you :)
Thanks man, great video!
How can we know that the poly T tail won't bind to the poly A tail of another mRNA molecule rather than the mRNA molecule of interest since all the mRNA molecules have a poly A tail
You don't. Poly T-tails will be used for non-biased cDNA library generation. If you want to make gene specific cDNA only, you will need a 3' reverse primer that is sequence specific.
Very good 👍
A question, E. Coli can produce RNAm eukariotic? What happen with the rbs of the eukariotics cells? In your video we watch that.
Excelent video, but shouldn't the 5' ends of the molecules have a phosphate group instead of a hydroxyl group?
i was wondering the same thing
Upload a video explaining adapters and linkers please
Excellent !
well explained
brilliant ! thanks a ton :)
Gayathri Jaikumar you're welcome!
Maybe already have long time this video but I have a question, and I hope someone can answer me. There's no technology to create the protein using our eucaryotic mRNA and to avoid the other steps to get a protein (eucaryotic)? I mean, if we can extract the mature transcript (modified mRNA -3' poli A tail, 5' cap and spliced exons) from a eucaryotic cell, why we need to form, make or any word you want to use, cDNA and then use procaryotic cell?, unless there's no technology to use directly the mature mRNA. Thanks
because retroviruses can insert specific DNA fragments into the host original DNA, this means everytime the host cell reproduces it will have this new modified DNA which can be translated in the new RNA , if you directly insert mRNA it will only be used once because mrna degrades over time
Thank you so Much
great vid!
Artur livshits thanks! :)
We can also use RNAse H to degarde that mrna?
thank you!
Very useful... thank youuu !
Thank you
Awesome! It helped me a lot.. thanks =)
Thank you soo much! you are genius :) So helpful
screenshot 6:37 for the notes :)
since the revierse transcirbatse need a primier to start doing its jop whats if we inserted a normal dna polymerase instead of the RT wouldnt it do excatly the same process of making complementry dna to the mrna ?
I don't think DNA polymerase will do its job on a single-stranded mRNA, as it cannot use the mRNA strand as some sort of 'template strand' (mRNA strands contain uracil instead of thymine). So that is why reverse transcriptase is used
Wow! Genius!
THANK YOU SO MUCH
Hi. I want to sintetize de Oligo dt(12-18) and Random Primers (d6)n and save some money. How do I have to configure de requirement (order) for the primer syntesis lab? I know that the 5' of the oligo dt must be modified wiht a PO3, but that's all I know lol... Please, help me
dude thank you
you mentioned a poly-C tail. did you mean poly T?
SLIP OF TONGUE
very great thank you.
i appreciate you!
Please I want to ask U a questiion ..Can I ?
Why couldn't we just directly isolate a DNA sequence that will produce the required mRNA? Instead of going from mRNA to cDNA to go back to mRNA that is needed?
OMG Thanks a lot!!
Thank you so much :)
Biology jargon! I love it...lol