if i have 2 mice genotype F/f;G/g;H/h;I/i;J/j are mated how many different phenotypes will be found? complete dominance of all. i can't figure this out, i can do it with, AaSs x AaSs, please help
It really depends on the cell type but in the industry, it is common to use viral vector, AAV, lipofectamine or even electroporation for the transfection.
When expressing proteins in E .coli the cells that have been successfully transformed can be selected for by antibiotics (if the plasmid being transformed contains antibiotic resistance). Do you know how we can select for the eukaryotic cells that have been successfully transfected?
Usually when we perform the transfection, we incorporate fluorescent reporter genes as well, so we can then screen the plate for the desired product. Also, running ELISA can help characterize the protein produced from the transfected cells.
I dont know if its an unusual request ... I didn't watched this video tho.. can you explain what are "p-pi p-pi and p-pi d-pi multiple bonding" thank you
Thank you so much Andrey, your lectures are helping me prepare for a very important admission test without going crazy and I'm so thankful for that!!!
job very well done. Thanks a lot!
is there any video that explains microarray technique?
if i have 2 mice genotype F/f;G/g;H/h;I/i;J/j are mated how many different phenotypes will be found? complete dominance of all. i can't figure this out, i can do it with, AaSs x AaSs, please help
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Is the injection w/ micropipette an example of transfection?
Which of those three methods is the most effective?
It really depends on the cell type but in the industry, it is common to use viral vector, AAV, lipofectamine or even electroporation for the transfection.
When expressing proteins in E .coli the cells that have been successfully transformed can be selected for by antibiotics (if the plasmid being transformed contains antibiotic resistance). Do you know how we can select for the eukaryotic cells that have been successfully transfected?
Usually when we perform the transfection, we incorporate fluorescent reporter genes as well, so we can then screen the plate for the desired product. Also, running ELISA can help characterize the protein produced from the transfected cells.
I dont know if its an unusual request ... I didn't watched this video tho.. can you explain what are "p-pi p-pi and p-pi d-pi multiple bonding"
thank you
pi bonds between two p orbitals are called p-pi p-pi bonds, and pi bonds formed by one p orbital and one d orbital are called p-pi d-pi bonds.
Can you explain also subjects about midical physics???
Tahajod Waleed one step at a time buddy.
+AK LECTURES (Andrey K) hahahahahaha
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K
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