2) Cell Culture - Recombinant Adenovirus Expression System

What is recombinant adenovirus expression system?
➜ Recombinant Ad expression vectors exploit the high nuclear transfer efficiency and the low pathogenicity of the virus to deliver genes to the host cell. Adenovirus vectors can be used in vaccines where the vector expresses a foreign antigenic protein and adenovirus vectors can be used in gene therapy, where the vector expresses a functional protein to correct a genetic defect.
For more information on the recombinant adenovirus expression system and for a list of the sources used, please visit:
➜ Knowledge Base: info.abmgood.com/adenovirus-s...
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➜ Portuguese
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➜ Cell Culture - An Introduction: • 1) Cell Culture Tutori...
➜ CRISPR Cas9: • CRISPR Cas9 - A Brief ...
➜ Next Generation Sequencing: • 1) Next Generation Seq...
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[@khalid_ba]

Given your organization's expertise in this field, it would be very helpful if you create one short video for every major adenovirus based vaccine for COVID-19 (Oxford/AstraZeneca, Janssen/Johnson and Johnson, Sputnik V, SinoVac, ..etc.) and what technology each uses, and potential drawbacks (e.g. existing immunity for adenoviruses, ...)

[@abmgood]

Hello Khalid,
We will definitely let our content production team know! Thanks so much for your suggestion! :)

[@akshaypal6753]

i support u on this , i mconcerned about link of hiv and ad5 vector in sputnik vaccine , we will like to see a vdo on this

[@swatijain6343]

This video is so much helpful thank you for making this video.

[@abmgood]

Hello Swati,
Glad to here that you found the video helpful! :)

[@JasonLubega]

Very well explained introduction to Adenoviruses as vectors.

[@shaunjoe7710]

Brilliant video. Good job.

[@abmgood]

Hi Shaun, thanks for the kind comment!

[@zunairaehsan5560]

great lecture helps me alot..
please also do lecture video on clone by clone shortgun sequencing

[@abmgood]

+zunaira ehsan Thank you for watching! I will add that topic to our list of videos and you can look forward to it. Be sure to subscribe if you haven't done so yet.

[@christinaboivie7943]

Applied Biological Materials - abm u
A ..•x

[@afriyantisandhi8500]

Great Video, Thank You !

[@abmgood]

Thanks for watching! ;)

[@Leongreat]

Great video! Quick qurstion. How the adenovirus vaccine capsid enters nucleus? Through what mechanism? Thanks!

[@edthoreum7625]

1:35 endocytosis, the nucleus has a certain gravity or magnetics?

[@christiandebou555]

hi, quick question: what generation of adenoviral vectors is most often used for gene therapy, or medical biotechnology.
(is 3rd gen lost used or are all generations used, i know that with lentiviral vectors the 2nd generation is most used because of the more effective reploduction in vitro due to needing less plasmids in the helper cells than the 3rd generation and beeing safe enough to use)

[@ggerdagg]

Does ad5 provide hiv?

[@vaishsejal3291]

what is the first generation and second generation , does this mean step 1 and step 2 here ?

[@abmgood]

Hello Vaish,
The terms first and second generation describe two different types of adenovirus vectors. First generation adenovirus vectors are made by substituting the adenovirus E1 and/or E3 transcription units for an expression cassette (transgene or therapeutic gene) under a high activity promoter. Second generation adenoviral vectors lack even more non essential regions, such as E2 and E4 transcription units, to increase the capacity for exogenous DNA and reduce the immune response.

[@ahmedmakhlouf9555]

what is the application you are using?

[@abmgood]

Hi Ahmed, could you clarify your question? If you are asking what software we use to create the videos, we mainly use Adobe Aftereffects for the animations.

[@irfanbeg9170]

I want need ,, how can I get this

[@jazryllejeanjumantoc7940]

where did you edit your video?

[@abmgood]

Hello there,
We use Adobe After Effects to edit our videos! :)

[@mishalwaseem952]

high nuclear transfer efficiency meaning?

[@abmgood]

High nuclear transfer efficiency simply means the virus has high transduction efficiency into the nucleus of the cell, making it a great choice for hard to transduce cell lines for transient expression. Hope this helps!

[@fabianmay9601]

what about HF-GLAd ?

[@edthoreum7625]

3:45 hek393&