Cell Immortalization: How to Immortalize Cells
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- Опубликовано: 19 май 2024
- Don't let your cells die before the end of your project! In this video, learn how to develop an immortalized cell line from primary cells.
Read our blog post for more information on cell line immortalization:
➜ info.abmgood.com/cell-immorta...
Timestamps for topics covered in this video:
[0:29] What are immortalized cells?
[1:19] How do you generate immortalized cells?
[3:47] Cell line quality control considerations
You’re probably used to using cells that are taken directly from living tissue, called primary cells. The difficulty with primary cells is that their telomeres shorten after every cell division, causing the cells to enter senescence and stop dividing after only a few cell cycles. This means that if you are working on a long term project, you’ll frequently need to keep harvesting and re-establishing new batches of primary cells. In addition, every batch of cells is different due to different harvesting conditions, making reproducibility a headache!
Immortalized cells (also called continuous cells or cell lines) are primary cells whose telomeres and/or tumour suppressor genes have been altered. Tumour suppressor genes (e.g. p53 and Rb) are important for signalling the cell to stop dividing when the likelihood of DNA damage is higher (i.e. after multiple cell cycles). In the case of immortalized cells, these genes have been knocked down or their function inhibited so that the cell is able to keep dividing indefinitely.
In this video, we will cover two major cell immortalization strategies: (1) Telomerase Reverse Transcriptase protein (TERT) expression, and (2) viral oncogenes. We will also introduce you to two immortalization methods (lentiviral transduction and plasmid transfection).
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If you want additional information on this topic, check out our latest blog post, "Your Basic Guide to Cell Line Immortalization": info.abmgood.com/blog/your-basic-guide-to-cell-line-immortalization. If you have any questions for us, feel free to leave them in the comments!
Can this be applied to car T cells after and before editing with crispr the sample
Cell immortalization reagents can be applied to cells before or after editing with CRISPR. Kindly note that cells have a higher chance of immortalization at lower passages. For more details, please contact our technical team (technical@abmgood.com) to further discuss your project.
I am a master's student and your videos have been super helpful. Will be sharing with all of my peers. Keep up the good work!
Thanks for watching! We are really glad to hear that you found our video helpful.
this is nice. reminds me of henrietta lacks.
I'm building my own genetics lab at home!!! Some fee lab stuff will always help!!!! Just bought 4 Nest 5-Layer Cell Culture Flask, Vent Cap Straight Neck
So exciting!!! How did it go??
Can't wait for them to get here!!!
Thanks very much for is valuable vedio. Could you please explain to me how can I obtain NRK-52E cell from rat directly to culture media
Hi, thank you very much for this video and others. Do you have any idea about neonatal intestinal epithelial cell line, is it immortalized or not and which method has been used for this cell to continue proliferate?. Best and good luck. Mahmoud Agena.
Hello there :)
To best support you, can you provide the reference of the neonatal intestinal epithelial cell line you are referring to? For a faster response, please contact our Technical Support Team directly at technical@abmGood.com.
great job was very helpfull
Glad it helped!
immortalized cells are cancerous cells to grow cultured or cell-based /artificial meats. As reported by The Fern,1 "Immortalized cells are a staple of medical research, but they are, technically speaking, precancerous and can be, in some cases, fully cancerous."
I made cell immortalization part of my morning routine and was able to cut my coffee consumption almost in half!
i'm wondering about exist of continuous genotype cell lines that also be immortalized
We are not sure the meaning of "continuous genotype cell lines", can you please elaborate? Do "continuous genotype cell lines" mean polyploid cell lines or the presence of polyploid cells in the immortalized population? Yes, polyploid cell lines can also be immortalized.
If you are referring to the latter, hTERT immortalized cells are mostly diploid, but may become pseudo-diploid especially at high passage numbers. In many cases, when cells become pseudo-diploid they still retain most primary cell functions.
Could this keep cells from dying inside the human body?
Yes we call it neoplasm
I would like to volunteer to try immortality process. I’m serious I’m not messing around. I’m real desperate. Please reply if you got my message.
Ok
For lentiviral which viruses are used?
Hi Dure, can you clarify your question? Lentiviral vectors are typically used with recombinant lentiviruses for gene delivery!
I was talking about proteins and genes related to immortalisation once to my family, I forgot their name so I said 'probably called TER56' 😂😂😂😂 damn I mixed them and I was wondering which one I forgot
Would you be able to immortalise every cell in the human body and therefore become immortal
Nope, I mean you can extrapolate from the video that you will need very fined tuned immortalisation techniques for every unique cell type your body has.
TL;DR you'll kill yourself trying to immortalise one group of cells, by killing another group
Yeah, it is called cancer
@@DerrickMrThomas 😔 dangerous
Hello and thank you for your very educational explanations.
I discovered your video following this one, on Henrietta Lacks' immortal cells.
ruclips.net/video/22lGbAVWhro/видео.html
So I was wondering : since it seems that scientists already have access to "ready-to-use" immortal cells, in what case is your way of immortalizing cells more interesting than using HELA cells ?
Especially as I've discovered before that the hybridomas, which involve fusing antibodies to cancer cells also to create an immortal cell line (capable of producing the useful monoclonal antibody).
ruclips.net/video/flv8PY43yYw/видео.html
ruclips.net/video/cCeze8tA_XI/видео.html
So, here again, I wondered : isn't it possible to do this with all the cell types you want to immortalize ?
And if so, in which cases would you prefer your technique to this one ?
I'm new to the subject, so my questions are probably clumsy, but it piqued my curiosity, so I'm going for it.
Thanks again for your work.
PS : Also, wouldn't it also be possible to use tumor organoids ?
no reply sad
Finally, the cure for death
Not exactly YET, keyword. Cell line immortalisation is essentially extending the telomeres, this doesn't fix defective genes or mutations. Best part is hTERT dna and viral oncogenes don't guarantee 100% immortality, your body needs a good 90% success rate and in certain critical organs (heart and brain tissue), a near 99.999% success rate. We in fact still don't fully understand the entire human body.
You may wonder, isn't a 20% margin of error acceptable? Well yeah, just cut off one of your arms or donate eyes.
This is where modern nano material science and GMOs can come in. We can create nano materials (nano particles, viruses, nano-bots) capable of monitoring and ensuring 99.999999999% success in immortalisation plus the benefit of cancer prevention by repairing or excising faulty genes, in every single one of your trillion cells, now and in the future.
This technology is very far away but physically possible, i'm talking easily another 300 years in precision material engineering and lithography. But, you may be able to extend your life by 5 years in the next 20 years!
@@gamingrex2930 Thank you for the reply, really appreciate it