Gene Silencing Methods: CRISPR vs. TALENs vs. RNAi
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- Опубликовано: 2 сен 2019
- Are you looking to perform a gene silencing project? Should you use CRISPR, RNAi, or TALENs to get the job done? In this video, we’ll explain how each system works and how they differ in terms of experimental set-up, efficiency, off-target effects, and more. You'll learn some important considerations when setting up projects using each system and some common applications.
The first thing you need to know is the difference between a gene knockout and a gene knockdown. A gene knockout is when a gene has acquired a frameshift mutation such that the cell no longer expresses any functional protein. A gene knockdown is when gene expression is reduced but not completely eliminated. CRISPR and TALENs are used to perform gene knockouts or introduce genetic mutations whereas RNAi is used for gene knockdowns.
Some systems, such as CRISPR and RNAi are easier to design whereas other systems such as TALENs can be more labor intensive. Some systems such as CRISPR and TALENs can be modified to expand their uses in gene manipulation whereas other systems such as RNAi are more restricted in their uses. Similarly, some systems such as CRISPR can be modified to reduce off-target effects, whereas other systems, such as RNAi can cause significant off-target effects.
We hope this video helps you decide which system is best for your gene silencing project!
For more information about the CRISPR, TALENs, and RNAi gene silencing methods, visit:
➜ info.abmgood.com/crispr-cas9-...
For a list of theoretical and practical CRISPR resources:
➜ info.abmgood.com/crispr
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Wow fantastic video! Well structured and explained, and great animations to go with it. The narrator also speaks very clearly. Brilliant!
Hi, thanks for watching and leaving a comment! Please let us know if you have any questions - we'd be glad to help!
@@abmgood good to understand basic comparisons
This video is so easy to listen to - I'm in the biotech industry and this is incredibly helpful background, thank you! Do you have other channels you admire that also break down cell & gene therapies?
Its the best explanation I've ever seen.Thankyou so much for the video. Keep posting
Thanks for watching! Please check out our other videos too! :)
outstanding explanation. Kudos to everybody involved in this project.
woow if only there is more videos like this one,,, many thanks from Saudi Arabia
No problem! :) Glad you found the video helpful!
👍👍
Thank you very much for this amazing comparison of these three mechanisms of gene editing
Glad you found the video helpful! ;) Thanks for watching.
Great video, thanks for posting and helping promote scientific literacy!
You're welcome. Thanks for watching!
Wow. Great explanation. Thanks for making this video.
No problem :) Thanks so much for watching!
omg this channel is so underrated 😩
Great explanation 👏👏👏well structured with beautiful narration and animation.
Thank you so much for watching! Stay tuned for upcoming videos as well ;)
Thank you so much! This helped me a lot :)
You're welcome!
Nice animations... Keep making such videos... Thank you
Thank you for your kind feedback!
Wow... Fantastic.. great explanation nd well articulated... Wishing you more success 👍
Thanks so much! Glad you found the video helpful ;)
wonderful, easy to understand
Glad you found it helpful!
very clear voice.
非常喜欢这个频道。谢谢你们的贡献!
Hi arabstaff1, thank you for watching and leaving a comment!
Applied Biological Materials - abm thx alot
Very useful video make more videos
that is great thank you!
Thanks for the video
Thanks for watching!
thank you!!!
You're welcome! :)
Awesome content
Thank you! :)
Please explain application of these techniques in various deseases.
I would also like to see a Video about Auxin inducible Degron Technology (AID)
Awesome
Thanks for watching!
Question
Does Gene editing means knockout target genes and gene sliencing means knockdown genes? So crispr and talen is different with RNAI
Thanks a lot ! Why you didn't compare also with the Cre-Lox System ?
Hello Setha,
Thanks so much for the comment! We will definitely let our content production team know your suggestion! :)
Thanks very much. And the cream of the crop would be to tell people WHICH companies are best positioned to which medical breakthrough and therefore WHICH STOCKs will perform best.
Help my name is maria i have two boys with duchenne one21 and 15. Help please deletion 46-52 i li e in nevada i would go any were to find a cure for them
rnai is amazing
Hi iam ARUNKUMAR PhD scholar from iit Hyderabad india I want to knock down an enzyme producing gene which one is best technique in terms of expenses and time requirements.do u have experts who help how to plan research
Hello Arun,
Please contact us at technical@abmgood.com to discuss your project in detail.
Thank you! :)
i feel so smort boi
Subtitle to spanish please???
Hello Cesar,
Unfortunately, Spanish captions and translated subtitles are currently not available. We will let you know for sure if it becomes available in the future. Thanks again for your interest on our video.
Está bien, entiendo, espero el futuro para poder verlo subtitulado, a mí se me complica entender el idioma al escucharlo, es más fácil cuando está escrito en inglés, pero a veces no le entiendo, por eso me preguntaba si podían subtitularlo, muchas gracias por responder.
: )
Ya esta activa la traducción automática, solo edita los subtitulos :))
Vaccine.