Wonderful explanations. It is unbelievable how young you are and how well you know what you teach, apart from having excellent teaching skills. The world would be so different if teachers like you were teaching in our classrooms. Thanks for dedicating your life to this, thank you for helping us see things in science clearer, thank you !
I just want you to know that you're awesome. Every time I happen to stumble upon one of your videos, my comprehension level immediately goes through the roof. Thank you.
I must say a big thank you. you've helped through med school, with your lectures that are so easy to understand. i can literally listen to you and enter into the hall with pride and the results are always very fruitful. thank you so much for your lectures. it has helped me a lot.
Your videos are always of such high quality and grade it amazes me how knowledgeable you are! What is your occupation and job? I'm dying to know. Are you a teacher? Or are you doing this as a hobby. Either way, loving your videos. KEEP IT UP!
Do you know how much I love you, everytime I have a Bio test I come to you, and once the video is done, I understand that topic like the back of my hand. Thanks so much :)
Really clear explanations. Constant repetitions makes it really easy to follow and to retain the information. Love this teaching method, keep it up! Was able to watch at 2.3x and the pronunciation was still crystal clear!
I'm french and you really did help me with my homeworks. Your explanations are really easy to understand and it's clear enough in just one sentence. You saved me ! Thanks !
Maybe you cover this in a future lecture but you have glossed over the main difference between pBR322 and pUC18, and that is how you determine which cells have taken up a recombinant plasmid (as apposed to taking up just the starting plasmid). In pBR322 you must use replica plating to determine which cells have taken up recombinant plasmid vs which cells have taken up the starting plasmid. For example, if you were cloning into the tetracycline gene, you would first select with ampicillin in order to identify which cells took up the pBR322 plasmid. You would then check the ampicillin colonies to determine which ones were no longer tetracycline resistant. Ampicillin resistant and tetracycline sensitive cells will have the recombinant plasmid. If the cells are resistant to both antibiotics, then they took up non recombinant pBR322. With pUC18 you can tell in a single step which colonies took up recombinant plasmid because these cells will be ampicillin resistant but will not turn blue because the inserted sequence has disrupted the B-gal. pUC18 has several other advantages over pBR322 but the ability to screen for recombinant plasmid in one step instead of two is a major advantage.
Wonderful.Claps Claps Claps. I love the way you explained. Very simply you clear this thing in my head. Thank you. Iam waiting for more vidoes like how to read the DNA digestion, why we do it etc etc.
Thank you so much for your brilliance and excellent teaching when tackling an intricate and puzzling concept. . My university lecturers could learn so much from you. Please continue your wonderful work.
My professor just pointed at the slides with his mouse and talking. I had no idea what he was talking about! Just a bit of showmanship and passion and it's all making sense
thank you very much for these lectures. every day i watch these videos. i am a biochemistry student. and these videos help me a lot. again 1000 thanks. :)
thank you very much for these lectures. every day i watch these videos. i am a biochemistry student. and these videos help me a lot. again 1000 thanks.
my prof just explain the lecture yesterday, i didnt understood completely,now i can understand it even better than my prof 😂😂😂 , thank u thank u thank u u r perfect man god bless u
Thank you very much for your awesome teaching. May I ask you to teach other parts of genetic engineering, esp genome engineering and integration (such as lambda red, CRISPR, TALEN, Zinc Finger, and MAGE)?
Wonderful lecture! There is a small mistake I indicated at the end of the lecture (where the inactivating incorporation in the beta galactosidase site is explained). The solution will not turn blue in any case (either the cells successfully uptake the plasmid or not), because the beta galactosidase is inactivated. The only indication will be is if the recombinant DNA was successfully incorporated (no blue color then) or not (then the solution will turn blue).
Such a detailed explanation! At "Recombinant DNA: Genes and Genomes - A Short Course" book about the last section of your video says that the plasmid does not contain the whole beta-galactosidase gene back the part of the gene that produces the enzymes N-terminus (part a). We also have bacteria that contain the part of the gene that produces the other part of the enzyme (part Ω). When a and Ω are together they produce the active form of the enzyme. However, I do not understant why do we make our lives more complicated, but it seems that the first part of the gene is used for the production of a lot of biopharmaceutical products, and my question is why do we use only a part of beta-galactosidase gene and not the whole one? Is it because of the size limits?! Keep spreading your endless knowledge!
how can we get the little DNA fragment to locate into the plasmid if the restriction enzymes cut the DNA molecole without create a little fragment? I don't know if I express my doubt in a clear way...
What is the significance of inactivating the green gene and having the cell pick up that plasmid, when both the green gene and blue gene were activated to begin with? Wouldnt a cell want a plasmid that enables resistance to both ampicillin and tetracycline?
Wouldn't it be more efficient to add ampicilin to the solution since that's the gene you inactivated, or do the bacterial plasmids not have resistance for either one?
Wonderful explanations. It is unbelievable how young you are and how well you know what you teach, apart from having excellent teaching skills. The world would be so different if teachers like you were teaching in our classrooms. Thanks for dedicating your life to this, thank you for helping us see things in science clearer, thank you !
+dayhdez Thank you for those kind words! greatly appreciate that :)
dayhdez he made a mistake at 8:39 btw.
dayhdez I agree. Thank you so much!
Young??!?!?! He's well in his 50s
@@AKLECTURES nol
Your videos are THE most informative & clearly explained biology/biochem videos I've ever come across! Thank you so much!
I learnt more in these 14 minutes than 2 weeks of lessons, wow thank you
+Simona Peneva you're welcome! :)
I just want you to know that you're awesome. Every time I happen to stumble upon one of your videos, my comprehension level immediately goes through the roof. Thank you.
I must say a big thank you. you've helped through med school, with your lectures that are so easy to understand. i can literally listen to you and enter into the hall with pride and the results are always very fruitful. thank you so much for your lectures. it has helped me a lot.
I"m so glad I found this channel. thank you so much!!!!!
your videos lectures simply second to none on the internet.Always well explained.Thanks for all your videos
You're a very good teacher. I take my hat off to you sir, and believe me when I say that I am extremely critical.. You deserve acknowledgment.
YOU ARE AMAZING!!! Wish I knew about your channel 10 years ago!!
Your videos are always of such high quality and grade it amazes me how knowledgeable you are! What is your occupation and job? I'm dying to know. Are you a teacher? Or are you doing this as a hobby.
Either way, loving your videos. KEEP IT UP!
Do you know how much I love you, everytime I have a Bio test I come to you, and once the video is done, I understand that topic like the back of my hand. Thanks so much :)
Great source of information! Thanks for taking the time to spread education on recombinant DNA technologies.
ive been watching your videos for months. Thanks!!
Hat down! U just have this conscience that make you giving all what you have in a very easy way! A pharmacist follower from Algeria...
Thank you...
by far the best video on this material!
Really clear explanations. Constant repetitions makes it really easy to follow and to retain the information. Love this teaching method, keep it up! Was able to watch at 2.3x and the pronunciation was still crystal clear!
I'm french and you really did help me with my homeworks. Your explanations are really easy to understand and it's clear enough in just one sentence. You saved me ! Thanks !
You are the best.!!!You do amazing work! Thank you for sharing
This is really great. Precise and on point. great presentation
Maybe you cover this in a future lecture but you have glossed over the main difference between pBR322 and pUC18, and that is how you determine which cells have taken up a recombinant plasmid (as apposed to taking up just the starting plasmid). In pBR322 you must use replica plating to determine which cells have taken up recombinant plasmid vs which cells have taken up the starting plasmid. For example, if you were cloning into the tetracycline gene, you would first select with ampicillin in order to identify which cells took up the pBR322 plasmid. You would then check the ampicillin colonies to determine which ones were no longer tetracycline resistant. Ampicillin resistant and tetracycline sensitive cells will have the recombinant plasmid. If the cells are resistant to both antibiotics, then they took up non recombinant pBR322. With pUC18 you can tell in a single step which colonies took up recombinant plasmid because these cells will be ampicillin resistant but will not turn blue because the inserted sequence has disrupted the B-gal. pUC18 has several other advantages over pBR322 but the ability to screen for recombinant plasmid in one step instead of two is a major advantage.
This was so helpful..your explanations are so clear, Thank you!
Why am I just finding these lectures? wow love this
Thank you so much! It helped a lot. With your videos I will be now able to understand my classes. Thank you again and keep doing this
Even though I am only in the 4. min. of the video, you explain it in a much more understandable and fluent way than my professor. thank you so much
Great explanation, Great teacher skills!! This video was very useful to me. Thanks!!
Great explanations, much appreciated.
How does this only have 702 likes? THUMBS UP!! This is amazingly useful. Thank you for dedicating your time, hope you get some money from it!
Absolutely clear video of recombinant DNA. Congratulations and thanks a lot for sharing, keep it up!!!!!
Gabriel Amodeo Thanks Gabriel! I will :)
Thank you so much! It helped a lot. With your videos I will be now able to understand my classes. Thank you again and keep doing this 😊
This is just perfect. Thank you a lot.
Wow, I found it very helpful and the way you presented it is outstanding. Keep the good work
Wonderful.Claps Claps Claps. I love the way you explained. Very simply you clear this thing in my head. Thank you. Iam waiting for more vidoes like how to read the DNA digestion, why we do it etc etc.
The best channel to learn !!!!!! Thank you soooo much...
you are a great lecturer,you are making my studies of doctor of medical laboratory science great
wow thank you for explaining everything so damn clear !
Thank you so much for your brilliance and excellent teaching when tackling an intricate and puzzling concept. . My university lecturers could learn so much from you. Please continue your wonderful work.
Thanks AK you are one of best lecturer
You're a very talented teacher, thank you! :D
Awesome! Thank you
I learn a lot by watching your videos. Thank you very much !
+Dyane Ribbink you're welcome Dyane
It’s so clearly. Thanks a lot.
crystal clear!! thank you so much ak!!!!!!!!!!
Very grateful I found this video! Crazy how much I pay in tuition at my college and yet I learned much more from this video than from my professor.
Thanks for this, I learnt a lot, you earned a subscriber
My professor just pointed at the slides with his mouse and talking. I had no idea what he was talking about! Just a bit of showmanship and passion and it's all making sense
you are so awesome. Great detailed explanations
awesome as always
You are a great teacher!
Too good explanation, I an very thankful to you sir
you're so good AK!
You are a saviour!
Thank you so much ❤️❤️🌷you are amazing I wish all the best for you 🙏🏻I have an exam and your videos was so helpful
Best explanation ever..thank you..keep uploading :)
Wow ❤❤❤...im ....wow..this is wow .... I'm preparing for end of years this is awesome😊😊😊
He must be an Italian, just kidding. Your video is every effective, it's really help me a lot. Thanks
I find this very useful especially for us who missed molecular biology classes
you will be the reason i get my pharmD degree. you are amazing. thank you for all your videos!
you're welcome Sarah! best of luck with your studies!
did you get it?
top notch instructor!
So good!
Very helpful! Much needed for my upcoming exam
thank you very much for these lectures. every day i watch these videos. i am a biochemistry student. and these videos help me a lot.
you are amazing, i like your videos so much
Very helpful! Thanks!
thank you very much for these lectures. every day i watch these videos. i am a biochemistry student. and these videos help me a lot. again 1000 thanks. :)
thank you very much for these lectures. every day i watch these videos. i am a biochemistry student. and these videos help me a lot. again 1000 thanks.
my prof just explain the lecture yesterday, i didnt understood completely,now i can understand it even better than my prof 😂😂😂 , thank u thank u thank u u r perfect man god bless u
thank you
Thank you very much!!!!
Maa Shaa Allah.. Allahh give u vvvvvv.good mode ov teaching.. Thumbs up 👍 👍 👍 👍👍 4 ur tchng style
thank you!!
thank you!
Very nicely explained about the r DNA technology.
thank you so much ... 😄😄
you helped me alot in understanding molecular biology 😄
Really good lectures!!!
I like your explanation
Thank you so much that was awsome
Thanks sir. So informative.
Best wishes.
good lecture and explanation thank you very much.
It's important lesson in my study from ETHIOPIA.
Thank you very much for your awesome teaching. May I ask you to teach other parts of genetic engineering, esp genome engineering and integration (such as lambda red, CRISPR, TALEN, Zinc Finger, and MAGE)?
wish I'd known about this channel earlier
you are supper supper. i enjoyed this lectures and took notes like a student mean while am a teacher.
Thank you for explaining this in a way I can understand. I have a question, where are you from, Andrey? USSR?
Damn. This is good
Now I gain answer of my question topic...... Thank you Sir.......
Wonderful lecture!
There is a small mistake I indicated at the end of the lecture (where the inactivating incorporation in the beta galactosidase site is explained). The solution will not turn blue in any case (either the cells successfully uptake the plasmid or not), because the beta galactosidase is inactivated. The only indication will be is if the recombinant DNA was successfully incorporated (no blue color then) or not (then the solution will turn blue).
Great👍
thanks alot but can you please state the difference in transformation between gram +ve and gram -ve bacteria
Such a detailed explanation! At "Recombinant DNA: Genes and Genomes - A Short Course" book about the last section of your video says that the plasmid does not contain the whole beta-galactosidase gene back the part of the gene that produces the enzymes N-terminus (part a). We also have bacteria that contain the part of the gene that produces the other part of the enzyme (part Ω). When a and Ω are together they produce the active form of the enzyme. However, I do not understant why do we make our lives more complicated, but it seems that the first part of the gene is used for the production of a lot of biopharmaceutical products, and my question is why do we use only a part of beta-galactosidase gene and not the whole one? Is it because of the size limits?!
Keep spreading your endless knowledge!
thx this vedio is really helpful
Thanks for the lecture. How can I get the notes
must check it out!!!!!! explanations are mind blowing and interesting!!!!!!! go AK!!!!!!!!!!
you are awsome!
grt and best lecture..........
Thank you so much! Please come teach my bio class!
how can we get the little DNA fragment to locate into the plasmid if the restriction enzymes cut the DNA molecole without create a little fragment? I don't know if I express my doubt in a clear way...
What is the significance of inactivating the green gene and having the cell pick up that plasmid, when both the green gene and blue gene were activated to begin with? Wouldnt a cell want a plasmid that enables resistance to both ampicillin and tetracycline?
How do you distinguish between the transformants and the non transformants?
Thank you =D
Wouldn't it be more efficient to add ampicilin to the solution since that's the gene you inactivated, or do the bacterial plasmids not have resistance for either one?