yo' Bro. you don't know how much your videos have helped me to study for my molecular biology tests. I visit yoursite whenever I have a doubt. Thanks a lot.
Thank you so much Professor, it was fully packed with the clarity I was hunting for !! May God bless you abundantly for sharing your knowledge freely !!
Such an interesting and detailed video. I study Pharmacy and this will definately help me at Pharmaceutical Biotechnology's exam. I would like to give you an idea for another video: cosmids used as vectors, concatamer creation and the help of the enzyme terminase for the packaging at phage λ heads. Keep spreading your endless knowledge!
This was so amazing! Thanks a million for all the hard work you put in! Your explanation was precise and neat and perfect! i'm really grateful :) Sir do you have a video on lac operon? Please do share the link
hello!, I have a question.. at the end you say that we insert the fragment of recombinant DNA into the lambda phage, but where does it come from (the lambda phage)? has there been previously transcription and translation of phage DNA to make the capsid, if yes, so we need the material necessary for transcription / translation in the medium, it would therefore be necessary to add DNA polymerase, dNTPs and ribosomes, right?
Are we supposed to remove the genes involved in the lytic cycle of the phage for the purpose of cloning our DNA into E.coli cells? Is the vector then still viable for the gene to be inserted into E.coli DNA?
yes, but if I want to get the proteins which the inserted gene codes for, won't the viral dna have to be transcribed and translated within the host cell? In that case, does it really matter if the phage follows the lysogenic or the lytic cycle? (that is, if it gets incorporated OR replicates right away, in which case i suppose it will work much like a plasmid?). Are the terms lysogenic - lytic cycle even used in this case? Is there any danger for the host cell either way? OR is the phage used as a vector only in the case I want to create a genome library, not a cDNA library, so I only want it to go lysogenic? (genome library--> just study the genome, cDNA --> to receive polypeptide chains)
This is really helpful. may I ask, what is the advantage of lambda vector compare to viral vectors? Does it allow bigger DNA fragments (e.g. 1Mb) to be inserted?
The DNA inside the phages having 3 segments,if the segment 2 is taken out and our gene of interest is located in case of this the proteins which are produced by the segment can't produce inside the virus.Is this can't create any issues???
Yes, you need restriction enzyme digestion to cut between fragment 1 and 3, leaving 2 without attaching to the sequence, so how do you remove 2 before inserting the target DNA?
As a first-year med student, I love you and your lectures!!! Awesome, life-saving!!!!
yo' Bro. you don't know how much your videos have helped me to study for my molecular biology tests. I visit yoursite whenever I have a doubt. Thanks a lot.
Thank you so much Professor, it was fully packed with the clarity I was hunting for !! May God bless you abundantly for sharing your knowledge freely !!
I am a master student, and this helped me a lot to understand what actually I have to do in my project. TANK YOU
Instablaster
I struggled the whole semester with this! Thank you so much!
excellent! i wish i could have a professor like you! :3
you are more than perfect, you are my idol
honestly feel the same
Very neat and precise explanation. Thanks for uploading the video in HD.
very simple as well as more detailed , thanks prof👏👌
Such an interesting and detailed video. I study Pharmacy and this will definately help me at Pharmaceutical Biotechnology's exam. I would like to give you an idea for another video: cosmids used as vectors, concatamer creation and the help of the enzyme terminase for the packaging at phage λ heads.
Keep spreading your endless knowledge!
You explain things so well! Thank you!
Jasmine Cassis you're welcome :)
excellent lecture thank you
thanks this is really helpful better than reading my biochemistry book. God bless you brother thanks
YOU ARE AWESOME! Thank you so much for all of this hard work!
I had searchd a lot for this lacture...very usefull n well xplaind 👍
Ur explanation is just amazing sir. Understood it in a very better way. Thank you
Thanks a lot. You really eased my genetics revision a lot. 👍🏼
Thank you so much 😊 AK Lectures .....you explain very well...🙏
Thanks for making it really easy topic..
Thanks! very useful. How do you insert the recombinant DNA into the phage again?
Thank u 😄 u saved my exams 😅
I wish one can give a love on RUclips. Your videos are mind-blowing. Thank you very much and God bless you in Jesus Name 🙏 ♥️♥️♥️♥️♥️♥️♥️♥️♥️
Great explaination!!!
This was so amazing! Thanks a million for all the hard work you put in! Your explanation was precise and neat and perfect! i'm really grateful :)
Sir do you have a video on lac operon? Please do share the link
0316 uh, z as s km. N
hello!, I have a question..
at the end you say that we insert the fragment of recombinant DNA into the lambda phage, but where does it come from (the lambda phage)?
has there been previously transcription and translation of phage DNA to make the capsid, if yes, so we need the material necessary for transcription / translation in the medium, it would therefore be necessary to add DNA polymerase, dNTPs and ribosomes, right?
You explained it perfectly, so many thanks :D
E hmm
Awesome teacher
thanks alot it helped me for my assignment
Are we supposed to remove the genes involved in the lytic cycle of the phage for the purpose of cloning our DNA into E.coli cells? Is the vector then still viable for the gene to be inserted into E.coli DNA?
can i ask from where do we get a paspaper mcq for this subject ????
Excellent tutor😍😍😍
You safe my time , thank you
Sir, means video is only about amplification of viral + gene of interest
What about expression
Do you have a video explaining the use of cosmids as a vector?
Great video, thank you!
Masha Allah
Allah bless you
outclass explanation great sir!
yes, but if I want to get the proteins which the inserted gene codes for, won't the viral dna have to be transcribed and translated within the host cell? In that case, does it really matter if the phage follows the lysogenic or the lytic cycle? (that is, if it gets incorporated OR replicates right away, in which case i suppose it will work much like a plasmid?). Are the terms lysogenic - lytic cycle even used in this case? Is there any danger for the host cell either way?
OR is the phage used as a vector only in the case I want to create a genome library, not a cDNA library, so I only want it to go lysogenic? (genome library--> just study the genome, cDNA --> to receive polypeptide chains)
thank you for the great explanation!
Very clear.Thank you so much.
this is the greatest explanation video i have seen ever, thank you
Well explained
Thank you 😇
get all details well explained thank u sir
Sir, in lysogenic cycle after the bacteriophage ___________ the viral , whats the blank
You are amazing!
keep up the awesome work!!
Excellent, thank you very much
Thanks a lot dear sir🙂
Wow.. thank you so much😊
Interesting and simple
Thanks a bunch!
This is really helpful. may I ask, what is the advantage of lambda vector compare to viral vectors? Does it allow bigger DNA fragments (e.g. 1Mb) to be inserted?
Not possible... Coz size of lamda phage is around 40k to 53k... For 1 mb u can use YAC( yeast artificial chromosome)
Thank you!!
thanks for the explanation
Hlo sir.... U r outstanding sir.....much love sir 😍 😍 😍 😍
Awesome! Thanks!
when virus undergoes lytic cycle??under favourable or unfavorable conditions??plz reply
Occurs through UV INDUCTION (DNA damage), initiating an SOS response which will then cause cleavage of the C1 protein, turning on the Lytic Cycle
Whenever it utilize all the energy and protein of host cell then lysogenic cycle comes in more favor to lytic cycle...
great video
i have a question: how to insert recombinant DNA into lambda phage?
i think it's like in lytic cycle, so there is an encapsidation of recombinant DNA into the capsid
Perfect just the board not clear to take the notes thank you
The DNA inside the phages having 3 segments,if the segment 2 is taken out and our gene of interest is located in case of this the proteins which are produced by the segment can't produce inside the virus.Is this can't create any issues???
Out of 100% only 40% genes are useful for the lambda virus genome... Which is mostly cro/ci (useful genes) present left and right side of the genome
can the viruses go to the lytic cycle after we replace their DNA with our DNA of interest?
thank u sooooooooo much sir😇
Thank God i found your channel, now i feel prepared for my class test tomorrow :)
thanks alot
How do you remove fragment 2?
restriction enzymes
Yes, you need restriction enzyme digestion to cut between fragment 1 and 3, leaving 2 without attaching to the sequence, so how do you remove 2 before inserting the target DNA?
You're so cool
Jasmine Cassis thanks Jasmine :)
you are fucking awesome! thanka you not for only this video but for allll of them.
👍
i just realised he sounds a lot like sean evans from hot ones
also deliver a lecture on phagemids and cosmids
to basic, its for five years old kids? como on, we have to much level in Argentina
I will soon be able to gene edit my tissue in vivo. Then we'll see what ubermench look like.
what is that accent??
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Thank you 🙏