Construction of cDNA library | Synthesis of cDNA | rDNA Technology | Bio science

Construction of cDNA library | Synthesis of cDNA | rDNA Technology | Bio science
A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, of which constitute some portion of the transcriptome of the organism and are stored as a "library". cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an organism. Similarly, tissue-specific cDNA libraries can be produced. In eukaryotic cells the mature mRNA is already spliced, hence the cDNA produced lacks introns and can be readily expressed in a bacterial cell. While information in cDNA libraries is a powerful and useful tool since gene products are easily identified, the libraries lack information about enhancers, introns, and other regulatory elements found in a genomic DNA library.
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The name reverse transcriptase is due to synthasise of DNA from RNA not for 3' to 5 '

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Sir, can you plz resend the notes of this topic in the group... I just joined it

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[@mr.akshaykisanmundhe9269]

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Where is the link...not found in the description box

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[@mounikapolishetty9545]

Good explanation, but for the formation of mRNA only transcription occurs, translation is not done
And reverse transcriptase enzyme synthesizes DNA from RNA in 5' to 3' direction

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Good explanation bro I watched this vdo before my semester exm without knowing the concept. Now I know about cDNA construction . Thankyou

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Are the cDNA fragments single stranded or double stranded?

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Double stranded

[@khushivers3]

As it moves to 5 prime end will form hair pin loop

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Where do I find whatsapp link for the notes?

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I can't join the what's app group bcz it's full.....

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S 1 nuclease cleave

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Oligo dt primer

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Thymine nucleotide

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Dna polymerase

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those are not "cloned plasmids", it called as recombinant DNA

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They are also called as cloned plasmids

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Alkaline naoh

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Reverse transcriptase

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Sir whatsaap no. ?