I have the most important test of my life comming up in two weeks. your videos are helping me a lot. I'll come back to this comment once the results are in. thanks mr crux
I was a bit confused about the first part with the auxotrophy... So the host is auxotrophic for a compound because of a mutated gene so it is no longer able to create the compound coded for by the gene. Then the vector has a gene that takes over or works with the mutated gene so that the cell can begin creating the compound again? What about if they don't have a mutated gene and are not auxotrophic for the compound?
"Then the vector has a gene that takes over or works with the mutated gene so that the cell can begin creating the compound again?" Yes, you are correct. The vector supplements the deficiency of the host cell. "What about if they don't have a mutated gene and are not auxotrophic for the compound?" Then the host cell will never die. Thing of auxotrophic genes as analogous to antibiotic resistance. If the all cells were resistant in the first place then the presence or absence of vector is meaningless because the host cell is independent. Likewise, the yeast cells have to be dependent on something external for survival. If the vector cannot get into the host cell - the host cell dies = host is selected for death. If the vector gets in, it can overcome the deficiency and host lives = host is selected for survival. Edit: In these experiments, the cells are grown on minimal media. Only the mutant hosts containing vector can survive in this. Those who don't get the vector can't synthesize the missing compound in the minimal media - and they die.
Thanks for such an informative video! Your handwriting are so clear and well-organized, making me easily understand !
I have the most important test of my life comming up in two weeks. your videos are helping me a lot. I'll come back to this comment once the results are in. thanks mr crux
Very informative and well explanation
I was a bit confused about the first part with the auxotrophy... So the host is auxotrophic for a compound because of a mutated gene so it is no longer able to create the compound coded for by the gene. Then the vector has a gene that takes over or works with the mutated gene so that the cell can begin creating the compound again? What about if they don't have a mutated gene and are not auxotrophic for the compound?
"Then the vector has a gene that takes over or works with the mutated gene so that the cell can begin creating the compound again?" Yes, you are correct. The vector supplements the deficiency of the host cell.
"What about if they don't have a mutated gene and are not auxotrophic for the compound?" Then the host cell will never die. Thing of auxotrophic genes as analogous to antibiotic resistance. If the all cells were resistant in the first place then the presence or absence of vector is meaningless because the host cell is independent. Likewise, the yeast cells have to be dependent on something external for survival. If the vector cannot get into the host cell - the host cell dies = host is selected for death. If the vector gets in, it can overcome the deficiency and host lives = host is selected for survival.
Edit: In these experiments, the cells are grown on minimal media. Only the mutant hosts containing vector can survive in this. Those who don't get the vector can't synthesize the missing compound in the minimal media - and they die.
@@theCrux ok thank you!
awesome video
YIp must also have an yeast specifici promoter
Amazing explanation . Any recommended book.
Keep up the good work brother ❤