thank you so much for your videos🥳. I have a phD in Biohemistry and I have been watching all your videos to study for a very important test that I will have to take! Portuguese is my first language, btw😅 . Still, science is still universal! Your explanations are very clear and straight up to the point. Congratulations. Please keep recording!🥰
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@@animatedbiologywitharpan sure thing, I will definetly share with friends who are studying with me for the same test. I think the problem in Brazil might be the language barrier between portuguese and english (Brazilians prefer videos in portuguese because english is not spoken here). That's why your ilustrations and worflow are so important , and you do them very nicely. Please don't stop recording, even if not so many people are showing up in the numbers. Your explanations have been really valuable to me and to everyone who seeks knowledge. You have all my respect and admiration. Thanks a lot, Arpan.
Very good video. You don't miss a point to explain. Like also the positive and negative sites. Really helped me with exam preparation in gene technology. Go on like that :) really cool
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Can you make these videos a little slower and also add a screen when you tell advantages and disadvantages like hearing those details quickly is difficult,if they are written it would be very helpful, And Amazing effort far better than any other explanation videos
Thanks for the feedback.....it would be kept in mind for future....for now if you think the speed is fast you can slow the speed of video from settings
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Thank you sooo much! Your videos are really useful and I love the way you explain! And... actually english is not my native language (I'm from Colombia) and honestly, I find it easier to understand people with an accent so... good for me!! haha Anyway...keep going with this! please!!
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Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
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Nope I dont have a good video on that....will make a video on that soon ......please share my channel link with your friends and help me to reach big audience
@@Navya20046 I guess it's because linear vector resembles Eukaryotic chromosomes which are linear and have telomeres at the ends. Maby if we wanted to use it in mice it would have to resemble mice chromosomes to work properly.
@@Navya20046 Yes I think so. I found intormation thet a circular YAC vector is able to replicate in E. coli due to the presence of bacterial ori and bla gene and is able to propagate in yeast as a linear molecule containing all necessery chromosomal elements: CEN4, ARS1 and two TEL which are functional in yeast after linearization.
@@animatedbiologywitharpan Sure. Definitely 🙂.... This is what I need to share to you too.... Even I too started a new channel recently on biology topics so help me too reach good hights
As per Gene Cloning & DNA analysis by T. A brown 6E(P111), the presence of the insert DNA in the vector can be checked by testing for insertional inactivation of SUP4, which is carried out by simple color test, white colonies are recombinants & RED are not recombinants. While as per Analysis of Genes & Genomes by Richard J Reece: YAC in chapter Artificial chromosomes RED colonies are recombinants. Now what's correct
Red are recombinants. Insertional inactivation of SUP4 gene prevents suppression of ADE1 and ADE2 gene mutations. So now they will get mutated. Adenine synthesis will get interrupted and a red intermediate accumulates.
Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.
Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.
2:37 In real life this insertion (ligation) part never works. Instead you spend days to weeks troubleshooting. That's why I moved on to more efficient but slightly more expensive cloning methods like NE HiFi and Gap Repair. Of course, science being science those don't always work either. After 5 years in the lab and a worthless masters degree, I decided to go to medical school. Science wasn't worth it for me. I would've lost my mind if I had become a PhD student.
Yes sure. Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
After staying abroad I have some accent.... sorry if it bothered you... but it’s not something that I am trying to fake in order to portray myself as a cool guy..
![[4K] Watch SpaceX Catch A Starship Rocket From Space!!! #IFT5](http://i.ytimg.com/vi/pIKI7y3DTXk/mqdefault.jpg)
You have really worked hard for the content of the video and it really worked. Very presentable and interesting images, animations....Keep it up👍👍🙏👏
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One of best explanation..... Many people had made very lengthy lactures but their content is not Worthwhile ... Best job... And best content...
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sir its awesome..u have explained in the most practical terms ..these animations will definitely help us retain information in better way..
If you find this explanation useful please share my channel link in your college group and help me to reach big audience
thank you so much for your videos🥳. I have a phD in Biohemistry and I have been watching all your videos to study for a very important test that I will have to take! Portuguese is my first language, btw😅 . Still, science is still universal! Your explanations are very clear and straight up to the point. Congratulations. Please keep recording!🥰
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@@animatedbiologywitharpan sure thing, I will definetly share with friends who are studying with me for the same test. I think the problem in Brazil might be the language barrier between portuguese and english (Brazilians prefer videos in portuguese because english is not spoken here). That's why your ilustrations and worflow are so important , and you do them very nicely. Please don't stop recording, even if not so many people are showing up in the numbers. Your explanations have been really valuable to me and to everyone who seeks knowledge. You have all my respect and admiration. Thanks a lot, Arpan.
this video helped me more in my research, really thank you so much Dr.
Please share my channel link with your friends and help me to reach big audience
Very good video. You don't miss a point to explain. Like also the positive and negative sites. Really helped me with exam preparation in gene technology. Go on like that :) really cool
Please share my channel link with your friends and help me to reach big audience
Thank you. For the insertion of gene of interest in yeast, we can use homologous recombination or only multiple cloning sites?
through multiple cloning site only but checkouy my video on gateway cloning which use the principle of recombination
All videos are great and the speaking is good, understandable. Thanks
Thanks a lot Maria, please share my channel link with your friends and help me to reach big audience
Well explained in a very short period of time thanks☺
ruclips.net/channel/UC4IpyopsGWSjaPACNTZLuqg please subscribe to my other channel
Life saver ❤ .......I have exam at 11am
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Great video! Thanks for helping me learn this content!
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
That’s a great job, and it would be greater if you add subtitles.
Thanks for the feedback...... I would take care about it in future
You are indeed a great teacher
youtube.com/@letscrackbioexams94?si=l2Di6Uc4hCYYa3ve support me by subscribing to my second channel
@@animatedbiologywitharpan subscribed
Helpful video sir..thanku somuch...make more videos like this please
such a high value content, thank you so much,,,,, wish you luck from libya
Please share with friends
Very helpful sir. I have a presentation this morning on vectors
James Aondoyila all the best for your presentation.......please share among your friends and help me to reach big audience
Also my today
Can you make these videos a little slower and also add a screen when you tell advantages and disadvantages like hearing those details quickly is difficult,if they are written it would be very helpful, And Amazing effort far better than any other explanation videos
Thanks for the feedback.....it would be kept in mind for future....for now if you think the speed is fast you can slow the speed of video from settings
If you like the explanation... please share among your friends and help me to reach big audience
Ur work really helpful for me. So thank you
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Sorry , i don't use any Instagram and Facebook.
It is indeed great video..made it so easy to understand!!!.. appreciate your work..thankyou
Sayani Das please share among your friends and help me to reach big audience
Thank you sooo much! Your videos are really useful and I love the way you explain! And... actually english is not my native language (I'm from Colombia) and honestly, I find it easier to understand people with an accent so... good for me!! haha
Anyway...keep going with this! please!!
Please share among friends and help me to reach big audience
Always happy to help
very good explanation , thank you for your videos
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Thank you from Egypt
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Great! 👍Please make a video on M13 phage life cycle
Thanks for your suggestion I would definitely try to make a video on M13
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Very informative....and your voice is very sweet ....plz make more videos....
Take a look at all the playlists...please share it among your friends
Excellent explanation
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Excellent explanation sir
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thank you sir, this section was very informative and easy to understand .
Glad to hear that... I try to keep it short as I don’t wanna bore anyone...
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I am not any sir ..... just Arpan is fine
@@animatedbiologywitharpan ok arpan
You are doing great work ! Carry on
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Won't recombinant colonies be white? Sup4 is inactivated, hence pink colour won't be seen
Thank you so much! This is a big help!
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Thanks sir it very help ful and nice visualization ✨️
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@@animatedbiologywitharpan ok sir ✨️
Excellent video i had ever seen
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
such an out standing explanations
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@@animatedbiologywitharpan yes ofcourse sir. Please uplode more videos
@@pritamprasad9317 sure, i would upload more
Very nicely explained
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Always the best!!!
Follow me on instagram and facebook
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Thank you Dr
But is there a video explaining the principle of selection of recombinant clones specifically in pYac 2 vector?
Nope I dont have a good video on that....will make a video on that soon ......please share my channel link with your friends and help me to reach big audience
Thank you... It's very helpful
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You´re my hero!
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Great video sirji
Please don’t call me sir...... I don’t believe in this fake hierarchy.... we are all learners and all on the same boat
Glad to hear that you enjoyed the video
bravo, great work.
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well done sir thankyou for this video
Nice explanation 👍
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Good animation......helps for good understanding
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Nice video in 5 minutes.
All my videos are like that.... don’t forget to watch the entire playlist
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when we are talking about yeast , its eucaryotic , so why we have a circular DNA , it must be linear no?
It is Linear after the space between the bam H falls off
Why centromere and telomeric sequence is important in YAC ??
Thank you for your help!!!!! Great job
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Why do we have to digest vector with BamHI to make it linear? Can't it stay circular?
Did you found the answer of this question?
@@Navya20046 I guess it's because linear vector resembles Eukaryotic chromosomes which are linear and have telomeres at the ends. Maby if we wanted to use it in mice it would have to resemble mice chromosomes to work properly.
@@magdalenaarendt5680 but if we want to use it in prokaryotes then... will we leave it as it is means circular?
@@Navya20046 Yes I think so. I found intormation thet a circular YAC vector is able to replicate in E. coli due to the presence of bacterial ori and bla gene and is able to propagate in yeast as a linear molecule containing all necessery chromosomal elements: CEN4, ARS1 and two TEL which are functional in yeast after linearization.
@@magdalenaarendt5680 OKkk thanks 🙂🙂🙂
Dmmm what a lecture 🔥🔥
Our mol bio playlist is the best . Checkout this playlist ruclips.net/video/2iyAaXsfuWQ/видео.html
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Brilliant👏👏
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@@animatedbiologywitharpan 👍
Thank you so much...This was really helpful to me....
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@@animatedbiologywitharpan Sure. Definitely 🙂....
This is what I need to share to you too....
Even I too started a new channel recently on biology topics so help me too reach good hights
All blessings and wishes for achieving you at greater heights....
Even I too follow your channel to understanding the concept easier....
Thank you
Good one 👍👍👍
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As per Gene Cloning & DNA analysis by
T. A brown 6E(P111), the presence of the insert DNA in the vector can be checked by testing for insertional inactivation of SUP4, which is carried out by simple color test, white colonies are recombinants & RED are not recombinants.
While as per Analysis of Genes & Genomes by Richard J Reece: YAC in chapter Artificial chromosomes RED colonies are recombinants.
Now what's correct
Red are recombinants. Insertional inactivation of SUP4 gene prevents suppression of ADE1 and ADE2 gene mutations. So now they will get mutated. Adenine synthesis will get interrupted and a red intermediate accumulates.
Appreciate. Thank you so much.
Nice explanation sir
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@@animatedbiologywitharpan InshaAllha
thanks for understanding yac
Please share among your friends
thank you very much.
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Thanks a lot.
Where is the link sir for further study about bac
Basavesh BN due to low sound quality it was removed but would be uploaded soon
Basavesh BN you can study about back from old primrose book
You have a mistake in this video
Recombinant colonies are white in colour and u have mentioned that they are red in colour.
Cool. Thanks!
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Best one😍🤗
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Amazing 😇
Watch my entire molecular biology playlist
@@animatedbiologywitharpan Thank you so much teacher... 🍂
2:37 In real life this insertion (ligation) part never works. Instead you spend days to weeks troubleshooting. That's why I moved on to more efficient but slightly more expensive cloning methods like NE HiFi and Gap Repair. Of course, science being science those don't always work either. After 5 years in the lab and a worthless masters degree, I decided to go to medical school. Science wasn't worth it for me. I would've lost my mind if I had become a PhD student.
I agree with you. Things are much difficult in reality than in pen and paper
Sir could you please add the English subtittle also
Yes sure. Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Nice
SHORT BUT AWESOME
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Glad to hear that it was useful for you
Very helpful
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nice video
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For detailed courses by me you can join Unaccademy
Thanks a lot 😊
Atharv Pethe please share among your friends and help me to reach big audience
Animated biology With arpan definitely 😄
Thank you sir
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Wonderful👍
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Great.
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Red colonies are non recombinants
Mean?
@@wanhiskandar6270idk wt she meant here maybe insertional inactivation
my exam will be tomorrow, now i am here
Share in your college group and help your friends as well.....all the best for exam
Thank you ❤️
nice
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Thnku dada
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Thanks for your compliments, glad to hear that it was useful
Sure dada
Thx for the video can u help me withe documents
Email me at Arpanparichha1994@gmail.com
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❤❤❤❤
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Samjh ni aya bhai kya fayada 5 min ka
Barry Gardens
wowwww
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👍👍👍👍👍
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6272 Legros Falls
👍🏻😌
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Adam Mount
you saved my ass, ty mate
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👍
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Sanford Street
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Parisian Isle
👍🏿👍🏿
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PlzZZzZ speak little bit slowly...
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🙏🏻🙏🏻🙏🏻🙏🏻
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Bayer Way
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Runte Coves
Yest Artificial chromosome
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I think your accent is artificial. Please don't modify it. Keep it natural. Your videos are good otherwise :)
After staying abroad I have some accent.... sorry if it bothered you... but it’s not something that I am trying to fake in order to portray myself as a cool guy..
Nice
Roopavani 963 please share among your friends