So what is the benefit of choosing a genome up and inserting it into different plasmids? Like what is the purpose of doing level five type things and what is the next step in that process?
Many reasons. Historically, it was how Human Genome Project sequenced the human genome - making BAC/YAC/PAC genomic libraries. Other reasons could be probe making, identification of genes, manipulation of genes or large subset of genes, discovering functions in specific regions of the genome etc etc. A lot of these implied applications will become clear as more videos come up in this series - good that you are already thinking of such things.
@lovisabjorck8699 Certainly, Gibson Cloning can do this (I am assuming you are referring to Genomic Library prep). The downside with Gibson is that it requires additional prep/materials which may be limiting + HiFi/Gibson reactions are not cheap + things are hard to troubleshoot if they fail. This video was only meant to explain things using traditional tools and how things have progressed historically with just traditional cloning, without invoking any fancy new methods. Gibson would be "fancy" :)
Babe wake up, theCrux uploaded.
the level of quality in your videos are amazing. thank you for your work.
Sir please upload continuously please
Could you please explain molecular markers? RAPD,AFLP, etc Thank You
So what is the benefit of choosing a genome up and inserting it into different plasmids? Like what is the purpose of doing level five type things and what is the next step in that process?
Many reasons. Historically, it was how Human Genome Project sequenced the human genome - making BAC/YAC/PAC genomic libraries. Other reasons could be probe making, identification of genes, manipulation of genes or large subset of genes, discovering functions in specific regions of the genome etc etc. A lot of these implied applications will become clear as more videos come up in this series - good that you are already thinking of such things.
@@theCrux ah thank you. What's a probe in DNA context? 😁
@@theCrux Could this problem not be solved with Gibson cloning?
@lovisabjorck8699 Certainly, Gibson Cloning can do this (I am assuming you are referring to Genomic Library prep). The downside with Gibson is that it requires additional prep/materials which may be limiting + HiFi/Gibson reactions are not cheap + things are hard to troubleshoot if they fail. This video was only meant to explain things using traditional tools and how things have progressed historically with just traditional cloning, without invoking any fancy new methods. Gibson would be "fancy" :)
Keep up the good work!