Thank you so much for this video, it was very helpful!! Your explanations are clear and very well structured, what makes them very easy to follow. I also love your handwriting and drawings, they look very good! Thanks for helping me prepare for my Genetics Engeneering midterm!! :)
10:05 the attL site and attR site... the word L and R do not come from Left and Right, right? If they are, how does it suddenly come with two attL sites in one Vector and two attR sites in another? at 05:38 they were mixed as L R and R L after B B' , P P' recombination.. So I think R-DNAi-L and L-ccdB-R recombination will result back in P-ccdB-P and B-DNAi-B right? And also after LR recombination 12:26 the names attB1, attB3, attB2 are just randomly given? So it is also fine to name them attP1, attP3 and attP2?
At 3:54 the L/R do come from Left and Right. At 5:38 the custom vectors contain B B and P P. At 9:57 the custom vectors contain L L and R R. At 12:26 the names are not truly random. There are attP and attB pairs called attP1/attB1, attP2/attB2 etc. Theirs products are likewise called attL1/attR1, attL2/attR2, etc.
@@theCrux Thank you for you kind explanation! Sorry I think I confused you... What I meant was 3:11 you marked a half block as B and B' and also P and P'. At 4:52 you wrote B B and P P but just as hole block each. So my questions is the name B B and P P as block just mad up by yourself? Another question is, at 5:38 you wrote L and R in the donor vector (L-ccdB-R) just as you described at 4:01 (attL-virus DNA-attR). They have some combination (B+P'=L and P+B'=R) given that the whole block of B and P are divided into B and B' / P and P'. So at the end there are L-ccd-R part in the donor vector and R-DNAi-L part in the final vector at 5:38. But how come you write two vectors with (L L and R R) at 10:09? Becuase after B P reaction the Donors include either R+L or L+R (05:40). OR it is just made up by yourself to exlain LR reaction so it results in the final product as B B and P P? So it has nothing to do with previous stories of lytic phase or the initial BP reaction because after BP reaction the vectors have either L+R or R+L... I hope you understand my point. Thank you for your answer in advance!
Thank you so much for this video, it was very helpful!! Your explanations are clear and very well structured, what makes them very easy to follow. I also love your handwriting and drawings, they look very good! Thanks for helping me prepare for my Genetics Engeneering midterm!! :)
Better guide to cloning is not exist :)
This was so helpful. Thank you! Preparing to transfect drosophila for the first time.
Thank you for uploading this!!
10:05 the attL site and attR site... the word L and R do not come from Left and Right, right?
If they are, how does it suddenly come with two attL sites in one Vector and two attR sites in another? at 05:38 they were mixed as L R and R L after B B' , P P' recombination..
So I think R-DNAi-L and L-ccdB-R recombination will result back in P-ccdB-P and B-DNAi-B right? And also after LR recombination 12:26 the names attB1, attB3, attB2 are just randomly given? So it is also fine to name them attP1, attP3 and attP2?
At 3:54 the L/R do come from Left and Right.
At 5:38 the custom vectors contain B B and P P.
At 9:57 the custom vectors contain L L and R R.
At 12:26 the names are not truly random. There are attP and attB pairs called attP1/attB1, attP2/attB2 etc. Theirs products are likewise called attL1/attR1, attL2/attR2, etc.
@@theCrux Thank you for you kind explanation! Sorry I think I confused you...
What I meant was 3:11 you marked a half block as B and B' and also P and P'.
At 4:52 you wrote B B and P P but just as hole block each. So my questions is the name B B and P P as block just mad up by yourself?
Another question is, at 5:38 you wrote L and R in the donor vector (L-ccdB-R) just as you described at 4:01 (attL-virus DNA-attR).
They have some combination (B+P'=L and P+B'=R) given that the whole block of B and P are divided into B and B' / P and P'. So at the end there are L-ccd-R part in the donor vector and R-DNAi-L part in the final vector at 5:38.
But how come you write two vectors with (L L and R R) at 10:09? Becuase after B P reaction the Donors include either R+L or L+R (05:40).
OR it is just made up by yourself to exlain LR reaction so it results in the final product as B B and P P?
So it has nothing to do with previous stories of lytic phase or the initial BP reaction because after BP reaction the vectors have either L+R or R+L...
I hope you understand my point. Thank you for your answer in advance!