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theCrux
США
Добавлен 14 дек 2018
Rigorously explaining and illustrating biological concepts.
If you would like to access resources and reading material used to make these videos or invest in the growth of the channel, please check out the Patreon page: www.patreon.com/the_Crux
If you would like to access resources and reading material used to make these videos or invest in the growth of the channel, please check out the Patreon page: www.patreon.com/the_Crux
dCas9: CRISPRi CRISPRa CRISPR-ON CRISPR-OFF (Beyond Cas9 Genome Editing)
References/Resources: www.patreon.com/the_Crux
All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf
What is CRISPRi (CRISPR interference)? What is CRISPRa (CRISPR activation)? What is a dCas9 and how to use dCas9 for CRISPRi and CRISPRa experiments? dCas9 can be fused with effector domains to regulate gene expression for functional studies. In addition, you can extend the CRISPRi/CRISPRa based tools to build permanent epigenetic modifications using CRISPR-ON and CRISPR-OFF. In this video, I discuss the details of dCas9 based genetic manipulations (and multiple iterations/generations of these tools) used to repress and activate genes and go beyond genome editi...
All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf
What is CRISPRi (CRISPR interference)? What is CRISPRa (CRISPR activation)? What is a dCas9 and how to use dCas9 for CRISPRi and CRISPRa experiments? dCas9 can be fused with effector domains to regulate gene expression for functional studies. In addition, you can extend the CRISPRi/CRISPRa based tools to build permanent epigenetic modifications using CRISPR-ON and CRISPR-OFF. In this video, I discuss the details of dCas9 based genetic manipulations (and multiple iterations/generations of these tools) used to repress and activate genes and go beyond genome editi...
Просмотров: 792
Видео
CRISPR KO vs CRISPR KI experiment using CRISPR-Cas9
Просмотров 568Месяц назад
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf How to design a CRISPR experiment? How to perform surveyor assay to check gRNA targeting efficiency? How to perform a CRISPR knock-out experiment? How to perform a CRISPR knock-in experiment? What is the difference between the steps to perform a crispr deletion and ...
![How to design and express CRISPR guide RNA [pX330 plasmid, Tutorial]](http://i.ytimg.com/vi/r4puDgc9rBs/mqdefault.jpg)
How to design and express CRISPR guide RNA [pX330 plasmid, Tutorial]
Просмотров 961Месяц назад
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf What is a typical CRISPR Cas9 protocol? What is the workflow of a typical CRISPR protocol? How to design CRISPR Cas9 guide RNA for a CRISPR experiment and clone crispr guides in a plasmid (pX330)? In this video, I discuss a typical CRISPR protocol workflow and prese...
Genome Editing with CRISPR-Cas9
Просмотров 6 тыс.2 месяца назад
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf What is CRISPR-Cas9 technology? How does CRISPR-Cas9 genome editing work? What is the mechanism of genome engineering with CRISPR-Cas9? What is a PAM sequence? Where is the PAM sequence location and what is its function? What is the protospacer adjacent motif? How d...
![CRISPR-Cas9-mediated Adaptive Immunity Mechanism in Bacteria [3 Steps of CRISPR-Cas system]](http://i.ytimg.com/vi/I0aWfgvpCyU/mqdefault.jpg)
CRISPR-Cas9-mediated Adaptive Immunity Mechanism in Bacteria [3 Steps of CRISPR-Cas system]
Просмотров 1,6 тыс.2 месяца назад
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf What is CRISPR-Cas9? How does CRISPR-Cas9 work in its natural context? How does CRISPR-Cas9 protect bacteria against pathogens by providing adaptive immunity? What are the different types of CRISPR systems? CRISPR-Cas9 is a Type II-A CRISPR system. In general, this ...
A Brief History of Genome Editing
Просмотров 5612 месяца назад
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf This video briefly discusses the history of genome editing and how historical events have led to the current state of the art. The video discusses parallel timelines that have interwoven to result in modern methods today. It all of course rests on the discovery of g...
![Protein Expression Vectors - tet-OFF and tet-ON (tTA/rtTA + Tet based gene regulation) [Part 5]](http://i.ytimg.com/vi/1PtIdAQoqME/mqdefault.jpg)
Protein Expression Vectors - tet-OFF and tet-ON (tTA/rtTA + Tet based gene regulation) [Part 5]
Просмотров 9873 месяца назад
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf What are the types of tet expression systems? What is a tet-ON and tet-OFF expression systems? How do tetracycline inducible expression systems work? This video discusses the regulation of protein expression using a tetracycline inducible or repressible based system...
![Protein Expression Vectors (pET vector) - Induction of Protein Expression (IPTG + T7 Pol) [Part 4]](http://i.ytimg.com/vi/JHRIA8v40qA/mqdefault.jpg)
Protein Expression Vectors (pET vector) - Induction of Protein Expression (IPTG + T7 Pol) [Part 4]
Просмотров 1,6 тыс.3 месяца назад
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf What is a pET vector? What is a pET expression system? How do pET vector expression systems work? What are inducible expression systems and how do pET vector systems work? What is the function of T7 RNA polymerase in pET vectors and how does T7 RNA polymerase contro...
![Protein Expression Vectors - Examples (Fusion Proteins, Insulin, and Cas9) [Part 3]](/img/1.gif)
Protein Expression Vectors - Examples (Fusion Proteins, Insulin, and Cas9) [Part 3]
Просмотров 6043 месяца назад
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf In this video, we explore some common protein expression vector designs and how they are constructed. These are helpful examples and applications of protein expression vectors to intuitively understand the protein expression vector construction. We discuss 5 example...
Protein Expression Vectors - Template, Codon Bias, Affinity Tags and Epitopes [Part 2]
Просмотров 5403 месяца назад
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf In this video, we explore how to obtain templates for protein expression vectors depending on the gene structure (intron and exon language). Prokaryotic genes are intron-less, whereas eukaryotic genes often have introns. Obtaining template for protein expression vec...
Protein Expression Vectors - Expression Host and Recombinant Proteins [Part 1]
Просмотров 9313 месяца назад
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf Protein Expression vectors are used to make protein from the template DNA. In this video, we discuss some of the common applications of protein expression vectors and the complications involved with protein expression when changing the host i.e. heterologous protein...
RNA Expression Vectors - Design, Applications, and Examples (In vitro Transcription)
Просмотров 4734 месяца назад
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf RNA Expression vectors (different from protein expression vectors) are used for the sole purpose of making RNA from a template DNA. In this video, we discuss some of the common applications of RNA expression vectors and their rules/principles of design. Finally, we ...
Golden Gate Cloning or assembly - Type IIS (BsaI) restriction enzyme based cloning
Просмотров 2,9 тыс.6 месяцев назад
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf Golden Gate Cloning or Golden gate assembly is a restriction enzyme based cloning that uses type IIS restriction enzymes. It is a powerful way to clone large numbers of inserts in a single reaction without the need of using multiple enzymes. This video discusses the...
Gateway Cloning: Simple and Multisite Gateway Cloning - LR and BP Cloning
Просмотров 2,6 тыс.8 месяцев назад
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf Gateway Cloning is a popular recombination based cloning method. It does not rely on restriction enzymes but instead uses integrases to recombine att sites between a final and donor vector. This causes an exchange of DNA segments that inserts the desired DNA into th...
TOPO Cloning - TOPO-Blunt, TOPO-TA, TOPO-directional
Просмотров 4,7 тыс.11 месяцев назад
References/Resources: www.patreon.com/the_Crux All videos on Genetic Engineering: ruclips.net/p/PL0Ymnd-zt4Ij9qp5ziU0TkyF42LUcgHQf TOPO Cloning (Topoisomerase-based cloning) is a commonly used method to clone PCR amplicons. TOPO Cloning bypasses the need of restriction enzymes and ligases. In this video, we discuss the three main types of TOPO-cloning systems - TOPO Blunt, TOPO TA, and TOPO dir...
Cloning with Restriction Enzymes (5 Levels) - Traditional Molecular Cloning
Просмотров 1,6 тыс.Год назад
Cloning with Restriction Enzymes (5 Levels) - Traditional Molecular Cloning
Plant cloning vectors - Disarmed Ti plasmid, Cointegrate vectors, Binary vectors - T DNA transfer
Просмотров 6 тыс.Год назад
Plant cloning vectors - Disarmed Ti plasmid, Cointegrate vectors, Binary vectors - T DNA transfer
Yeast cloning vectors - YIp, YEp, YRp, YCp, YACs - Shuttle plasmid vectors
Просмотров 6 тыс.Год назад
Yeast cloning vectors - YIp, YEp, YRp, YCp, YACs - Shuttle plasmid vectors
P1 Phage and PAC cloning vector - Pac sites, Cre-loxP, and SacB lethality
Просмотров 2,2 тыс.Год назад
P1 Phage and PAC cloning vector - Pac sites, Cre-loxP, and SacB lethality
F plasmid, BACs, Fosmid cloning vector - Fosmids are small BACs (Bacterial Artificial Chromosomes)
Просмотров 1,7 тыс.Год назад
F plasmid, BACs, Fosmid cloning vector - Fosmids are small BACs (Bacterial Artificial Chromosomes)
Lambda Phage vector, Cosmid cloning vector - spi phenotype and in vitro packaged lambda virus
Просмотров 7 тыс.Год назад
Lambda Phage vector, Cosmid cloning vector - spi phenotype and in vitro packaged lambda virus
Plasmid and Phagemid cloning vector - Selectable vs. Screening marker
Просмотров 4,5 тыс.Год назад
Plasmid and Phagemid cloning vector - Selectable vs. Screening marker
Dam and Dcm methylation (restriction independent methyl-transferases) affects restriction enzymes
Просмотров 2 тыс.Год назад
Dam and Dcm methylation (restriction independent methyl-transferases) affects restriction enzymes
Restriction Enzymes (Endonucleases) in Molecular Cloning
Просмотров 2 тыс.Год назад
Restriction Enzymes (Endonucleases) in Molecular Cloning
Genetic Engineering - Classic vs Modern methods
Просмотров 3,8 тыс.Год назад
Genetic Engineering - Classic vs Modern methods
RNA Polymerase III Transcription - Promoters, TFs, Initiation, Elongation, and Termination steps
Просмотров 4,4 тыс.Год назад
RNA Polymerase III Transcription - Promoters, TFs, Initiation, Elongation, and Termination steps
Prokaryotic Transcription Factors in Elongation and Termination (GreA, GreB, Mfd, NusA)
Просмотров 2,6 тыс.Год назад
Prokaryotic Transcription Factors in Elongation and Termination (GreA, GreB, Mfd, NusA)
Rolling Circle DNA Replication and Amplification - Plasmids and Bacteriophages (M13 + PhiX174)
Просмотров 12 тыс.Год назад
Rolling Circle DNA Replication and Amplification - Plasmids and Bacteriophages (M13 PhiX174)
Mitochondrial DNA (mtDNA) Replication - D loop Replication (deep-dive video)
Просмотров 14 тыс.Год назад
Mitochondrial DNA (mtDNA) Replication - D loop Replication (deep-dive video)
Telomere DNA Replication - Chromosome end replication problem in eukaryotes
Просмотров 6 тыс.Год назад
Telomere DNA Replication - Chromosome end replication problem in eukaryotes
Man u r crazy ur detailing, drawing explaining speed of video is 🫡
WOW! You make it so clear, thank you!!
thank you!!!
The explanation was so good . Thanks for the video 🤍
Amazing lecture sir. keep up the good work.🖤🖤
Amazing board work and nice explanation!!! Thank you...
Man..I love your videos...can you suggest any books for this topic??...It would be great if you suggest books in your descriptions.
Sir, I am a great fan of your videos . I have request. Can you please make a video on rRNA processing including ETS and ITS removal and other details
Hello from România, what i can do if i remove 300 pear tree with crown gall? How much years i cant plant in that area? In romania the enginner say 5 years to no plant nothing in that areea . Thanks.
yooo tysm!!!!!!
thank you ❤
great well explained
Big thanks from China!!!! I can still get the other techniques by reading review articles, but struggled to understand this one on my own. Real life saver!!!
Bro i am serious, you are literally saving my life. Why stop, please keep going. The best explanation I've ever seen and its in great detail, its perfect...
Sir, I am not clear how loop formation can solve the problem of incompatibility between primase and helicase. Could you please help me? 17:13
Crystal clear explanation, very helpful for exams
Is there any lab in your knowledge which offers PhD position in core genetic engineering ?
Very educative description! Thank you!
Amazingly clear video, thank you! Just a small remark for you: it's 'foreign', not 'foriegn' :)
I love how you discuss these lessons, I am a bit of a visual learner and your videos help me a lot <3
Excellent future effectors .........vpr's of 3 proteins called RTA - or synergistic activators
Lots of thanks. Awesome! You made my day. Just a few minutes ago, I understood the whole topic and was ready for my exam .
I appreciate your ability to take all the information about this subject and package it into a thorough lecture. Thank you very much.
Yes bro thank you so much! I’m also subbed on your patreon!
Epigenetic Replication: ruclips.net/video/HB4IAiYqdzA/видео.html CRISPR-Cas9 in Genome Editing: ruclips.net/video/7ESZTE6rjLI/видео.html CRISPR-KI CRISPR-KO: ruclips.net/video/f0Q6JKu8Xjk/видео.html
The Primase and helicase incompatibility was something that I struggled to understand! Thanks a lot for this video!!
Awesome
How tf only 800 views 😭😭😭
thank you so much this is a lifesaver. i've been reading the papers on golden gate and your explanation is so helpful. seeing everything drawn out makes it much easier to understand!
I read a textbook for this topic and got massive headache. Then afterwards watched your video understood everything thank you. It was really helpful.
Is this explanation masters level?
Thankyou for the explanation
Respect 🫡
Very nice explanation!
Where is there an explanation for how this gene has a unique identification?
Your voice is heavenly, and your videos are educational ASMR. Every complex detail is organised to perfection. I would rather watch your playlists for hours than read a book or articles for 15mins 🙏
0:06 "Bacteria face routine harassment from Bacteriophages" 😂👌
Thank you for the great videos.
bro this is gold
базара нет, шикарный видос
Accelerationism/transhumanism/anthrophocene
Sir please make a video on lytic and lysogenic induction in bacteriophage
😏😏😏
YAWA WALA NAKO KASABOT
CRISPR-Cas9 in Genome editing: ruclips.net/video/7ESZTE6rjLI/видео.html gRNA Design using CRISPOR and CHOPCHOP: ruclips.net/video/r4puDgc9rBs/видео.html CRISPRi vs CRISPRa: ruclips.net/video/bRlQcIAtThk/видео.html
Is it beacuse the glucose from lactose is far from the membrane where adenyla cyclase is so it can enter glicolisis before reaching the membrane therefore the inhibicion is low, in the other hand when glucose enters thru membrane proteins it is very close to adenilate cyclase
Very well made and extremely detailed video! It helped me understand the topic better so I could explain it in a test. Thank you very much, greetings from Brazil!
Absolutely love your lectures, i have been reading genes by lewins along with the videos and i have to sometimes yours are even more detail oriented than them.
I'm getting into yeast genetic engineering for a project I'm working on during my Ph.D. This is an extremely useful video on a topic that is not well discussed outside of review articles
CRISPR-Cas9 in Genome Editing : ruclips.net/video/7ESZTE6rjLI/видео.html CRISPR KO vs CRISPR KI: ruclips.net/video/f0Q6JKu8Xjk/видео.html CRISPRi vs CRISPRa: ruclips.net/video/bRlQcIAtThk/видео.html