I've struggled to visualize the different rolling circle mechanisms because I feel I lack spatial awareness, which, one needs to have an aptitude for, especially in Molecular Biology, but your comprehensive diagrammatic explanations have really helped me out in not just understanding but also rethinking how to begin to visualize these phenomena. Thank you for your hard work! I'll recommend your channel to anyone who is looking for help with/are interested in a deep dive in Mol bio!
May I ask a doubt?in case of phix174 why does the helicase/Dnab even assemble on to a single strand?is it because the primase can only assemble if helicase is present?
They assemble because of the primosome (Pri A B C) proteins. DnaT also aids in this assembly. Otherwise Helicase/DnaB/Primase would not bind the X174 ssDNA. For the assembly and interaction between primase and helicase, I suggest you take a look at Prokaryotic initiation assembly - there is more detail in the video than I can explain in the comment :) Playlist: ruclips.net/p/PL0Ymnd-zt4IgLefDaeqicJbCBnbLqnDJ7
I've struggled to visualize the different rolling circle mechanisms because I feel I lack spatial awareness, which, one needs to have an aptitude for, especially in Molecular Biology, but your comprehensive diagrammatic explanations have really helped me out in not just understanding but also rethinking how to begin to visualize these phenomena. Thank you for your hard work! I'll recommend your channel to anyone who is looking for help with/are interested in a deep dive in Mol bio!
Just Awesome, from start to end everything with in detail and perfection.
Thank u so much this helps a lot and sooo easy to understand.
sir plz made more videos because your way of explaining and data best
Thanks dear thecrux for this video... Big fan ....
Thank you so much ❤
Excellent presentation..could you please share the reference text if possible.
May I ask a doubt?in case of phix174 why does the helicase/Dnab even assemble on to a single strand?is it because the primase can only assemble if helicase is present?
They assemble because of the primosome (Pri A B C) proteins. DnaT also aids in this assembly. Otherwise Helicase/DnaB/Primase would not bind the X174 ssDNA. For the assembly and interaction between primase and helicase, I suggest you take a look at Prokaryotic initiation assembly - there is more detail in the video than I can explain in the comment :)
Playlist: ruclips.net/p/PL0Ymnd-zt4IgLefDaeqicJbCBnbLqnDJ7
Thank you 😊
😏😏😏