Hats off for this video. It's extremely helpful for understanding. Thank you so much. I have a small doubt. Can you please explain the exact meaning of 'contagious' and 'non-contagious?
When talking about ORFs, Contiguous = Continuous or Uninterrupted Non-Contiguous = Discontinuous or Interrupted For translation, ribosomes expect mature mRNA and therefore the ORF to be uninterrupted - which is true for bacteria at levels since they don't have introns; their ORF in mRNA = ORF in DNA. Eukaryotes (for intron-containing genes) have interrupted ORFs at DNA level and at the immature RNA level. Eventually, post-splicing, interruptions (introns) are removed to result in a continuous or contiguous ORF, ready for ribosomes.
![Protein Expression Vectors - Examples (Fusion Proteins, Insulin, and Cas9) [Part 3]](http://i.ytimg.com/vi/BhKzoIUH9_Q/mqdefault.jpg)
![Protein Expression Vectors - Examples (Fusion Proteins, Insulin, and Cas9) [Part 3]](/img/tr.png)
![Protein Expression Vectors - Expression Host and Recombinant Proteins [Part 1]](http://i.ytimg.com/vi/3_WnvVgIkYo/mqdefault.jpg)
Protein Expression Vector [Part 1]: ruclips.net/video/3_WnvVgIkYo/видео.html
Protein Expression Vector [Part 3]: ruclips.net/video/BhKzoIUH9_Q/видео.html
Protein Expression Vector [Part 4]: ruclips.net/video/JHRIA8v40qA/видео.html
Protein Expression Vector [Part 5]: ruclips.net/video/1PtIdAQoqME/видео.html
thank you very much for these videos
Hats off for this video. It's extremely helpful for understanding. Thank you so much. I have a small doubt. Can you please explain the exact meaning of 'contagious' and 'non-contagious?
When talking about ORFs,
Contiguous = Continuous or Uninterrupted
Non-Contiguous = Discontinuous or Interrupted
For translation, ribosomes expect mature mRNA and therefore the ORF to be uninterrupted - which is true for bacteria at levels since they don't have introns; their ORF in mRNA = ORF in DNA.
Eukaryotes (for intron-containing genes) have interrupted ORFs at DNA level and at the immature RNA level. Eventually, post-splicing, interruptions (introns) are removed to result in a continuous or contiguous ORF, ready for ribosomes.
@@theCrux Got it. Thanks for explaining.