Expression vectors | What is in an expression vector? | applications of expression vectors

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  • Опубликовано: 27 июл 2024
  • This video describes the key features of expression vectors and compare it to a plasmid.
    this video will clear up several questions like:
    What is an example of expression vector?
    Which is the most essential feature of expression vector?
    What are expression vectors PPT?
    How do you choose an expression vector?
    Is pBR322 an expression vector?
    What is the difference between cloning vector and expression vector?
    please stay tuned for more

Комментарии • 81

  • @turtlesonpancakes
    @turtlesonpancakes 3 года назад +14

    This video was very informative and incredibly helpful. Thank you for breaking down each component and the significance/importance of its presence.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 года назад

      Please share my channel link with your friends and help me to reach big audience

  • @anjirrumey
    @anjirrumey 2 года назад +3

    Hatts off to you man, you just summarized the whole chapter for me. Liked you video💗

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 года назад +1

      Please share my channel link with your friends and help me to reach big audience

  • @maxruphio5515
    @maxruphio5515 4 года назад +6

    Thanks you so much. Your videos are a life saver to understand complex topics. Really appreciate your efforts

  • @andrewboniface4753
    @andrewboniface4753 4 года назад +2

    Highly informative video.Keep doing more....!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 года назад

      Please share my channel link with your friends and help me to reach big audience

  • @hendriyajude6326
    @hendriyajude6326 2 года назад +2

    Amazing video.

  • @VirtualSolace
    @VirtualSolace 2 года назад +1

    Very nice video for understanding the expression vector 👍

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 года назад

      Please share my channels link with your friends and help me to reach big audience

  • @R.M.111
    @R.M.111 4 года назад +4

    Nicely summarized, thank you 👍

  • @BossManWebbMerica
    @BossManWebbMerica 4 года назад +2

    Better than most! Keep it up!

  • @kvnkvn9191
    @kvnkvn9191 4 года назад +2

    This specific video is specifically nice also it's specific

  • @deyocampo9880
    @deyocampo9880 4 года назад +3

    Thank you! helps me a lot!

  • @sidharthmundra9286
    @sidharthmundra9286 4 года назад +3

    very informative. Keep making videos :)

  • @AM-es1ng
    @AM-es1ng 3 года назад +1

    Amazing presentation, thank you

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 года назад

      Please share my channel link with your friends and help me to reach big audience .

  • @Hahahahahahahahasiu
    @Hahahahahahahahasiu 3 года назад +2

    May I have a question ,if the cDNA is from the eukaryotic organism ,Do we need to use the pcr primer to remove the RBS and the start condon first . As the bacterial ca not use the

  • @fireangel136
    @fireangel136 4 года назад +2

    Very helpful! thank you

  • @shailenderrajput3500
    @shailenderrajput3500 4 года назад +3

    U beauty sir simple and precise

  • @mohitsagrobiology3135
    @mohitsagrobiology3135 5 лет назад +2

    Nice work bro!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 года назад

      Please share my channel link with your friends and help me to reach big audience

  • @dr.daniaalkhatib3946
    @dr.daniaalkhatib3946 Год назад +1

    amaaaazing video,, thank you so much

  • @avijitchowdhury8577
    @avijitchowdhury8577 4 года назад +2

    Loved it

  • @bikrammajhi7278
    @bikrammajhi7278 3 года назад +2

    Thanks man!

  • @Andrei_2011
    @Andrei_2011 3 года назад +3

    dude i searched Wich restriction enzymes are required to clone the mammalian expression vector pMV2 BMPR?
    a there is no game question

  • @KumariSardaRani-pn5pm
    @KumariSardaRani-pn5pm 11 месяцев назад +1

    Can you please explain why Expression Vectors like BL21DE3 are poor choice for direct Cloning.

  • @khizerhayat9160
    @khizerhayat9160 4 года назад +1

    Really helpful

  • @galoisgalois65
    @galoisgalois65 2 года назад +1

    I understood more in this 6 minutes than my 16 week biochem course

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 года назад

      Thanks for your comment. Please share my channel link with your friends and help me to reach big audience. Checkout my entire playlist

  • @ayshaayshaa2149
    @ayshaayshaa2149 4 года назад +2

    Thank you 🙏

  • @rumamondal6046
    @rumamondal6046 4 года назад +2

    Thank u sooooooo much

  • @Sss38753
    @Sss38753 Год назад +1

    Plz tell me is the eukaryotic gene of interest only expressed through expression vector or prokaryotic gene also could be expressed?

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Год назад

      Both eukaryotic and prokaryotic genes can be expressed using the expression vectors but the type of expression vector is different.

  • @fizhaadnannn
    @fizhaadnannn 4 года назад +2

    Hi, can you explain about vector that has two origin of replication? why it is so?

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 года назад

      Most of them are shuttle vectors such that can be used in prokaryotes And eukaryotes at the same time... hence they have two origin...further watch my videos on shuttle vector

    • @fizhaadnannn
      @fizhaadnannn 4 года назад

      yeahhh i just found your video on shuttle vector, thank youuu

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 года назад

      Fizha Adnan good

  • @zhansayabauyrzhanova2492
    @zhansayabauyrzhanova2492 Год назад +1

    thank uu, i have a question: our insert gene how inserted if they do not have the same blunt nucleotides?

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Год назад +1

      Very interesting question, this should be a video on it's own.
      In short, Linkers and adaptors can be used which are oligonucleotides that are useful in DNA ligation during blunt end cloning. In cloning experiments the cleaved DNA often has blunt or sticky ends. Sticky ends are easy to ligate but DNA strands with blunt ends are hard to ligate. The linker and adaptor molecules then come into play.
      The linker and adapter molecules have both blunt and sticky ends and internal restriction sites as well, which help in DNA ligation.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Год назад

      In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

    • @zhansayabauyrzhanova2492
      @zhansayabauyrzhanova2492 Год назад

      so naturally our insert of interest itself has sticky ends and how do we know which sticky end Bam, Hind or which has?

  • @hafizanamal
    @hafizanamal 3 года назад +1

    Where is gateway cloning video?

  • @samarthkumar5198
    @samarthkumar5198 3 года назад +1

    How to check for gene expression in cancer cells by finding candidate genes?

  • @invincibleaquarian260
    @invincibleaquarian260 4 года назад +1

    Can we use pdest17 for Cloning and expression

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 года назад

      That’s a gateway cloning vector .... actually gateway cloning is much better than the restriction ligation based approach.... efficiency is much higher

    • @invincibleaquarian260
      @invincibleaquarian260 4 года назад

      @@animatedbiologywitharpan thanks. Can we directly insert our gene of interest or do we need to use pentr D TOPO

  • @bittukumarjha5244
    @bittukumarjha5244 Год назад +1

    shin dalgarno sequence found in prokaryotic mRNA..

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Год назад +1

      It's artificially provided in the vector because it helps recruit the ribosome to the mRNA to initiate protein synthesis by aligning the ribosome with the start codon. Goal of an expression vector is to express the protein in bacteria/ eukaryotic cell.

  • @Mustafaiq
    @Mustafaiq 3 года назад +2

    Hello, brother. I speak Arabic, can I get a pdf On the email ? Hello, brother. A nice explanation I speak Arabic can I get a pdf for the purpose ofTranslation

  • @bengregory4227
    @bengregory4227 2 года назад +1

    how did you do it can you share with me , thank you

  • @prajwaldongare9055
    @prajwaldongare9055 2 года назад +1

    If want to add promoter what to do ?

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 года назад

      You have to clone the promoter sequence along with your target gene and then put it in the vector. In general expression vector already has promoter sequence

    • @prajwaldongare9055
      @prajwaldongare9055 2 года назад

      @@animatedbiologywitharpan yes so.. I need to remove that promoter ? And if yes so there is no restriction site, in both side of promoter so how?

  • @chetnarai4096
    @chetnarai4096 2 года назад +1

    Can expression vector be linear?

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 года назад +1

      The most popular ones are generally circular. There could be some linear phage based vectors but i dont know much about them

    • @chetnarai4096
      @chetnarai4096 2 года назад +1

      Thank you

  • @TariqAziz-sg9fl
    @TariqAziz-sg9fl 3 года назад +1

    can you send this ppt

  • @jordanerickson701
    @jordanerickson701 4 года назад +6

    Thanks for info. You need a better mic

  • @JBulsa
    @JBulsa 3 года назад +1

    How Uv light 💡 bonds genes 🧬? Nitrogen 🔪 knife ? Nitrogen gas cold 🥶. P53 gene 🧬 cancer ♋️. BNGO$

  • @ShilpashreeGowdaRCZMI
    @ShilpashreeGowdaRCZMI 3 года назад +3

    Dude I can't judge you I am small in means* of information but u have improve ur talking skill👍