quick checking so what are the vector that's shuttles and expression? can I said YAC is shuttle type and how about BAC and other plasmid like pSC101,pBR322 and pUC >"
There are few reasons the vector back bone is double stranded while mRNA would be single stranded moreover it’s hard to isolate mRNA of a specific gene and mRNA is highly unstable
Miss AK it was not designed for that purpose.....key contribution of bac in biology was to make big genomic libraries....it was never used in gene expression purpose....all these vectors are designed for specific purpose and need so bac is not designed to shuttle between host
@@animatedbiologywitharpan you said that bac is not designed for that purpose then you said that it was designed to shuttle between hot (last line) I am confused
Yeast episomal plasmid or yep is also a shuttle vector with much lower carrying capacity than the yac...... actually I have a video on that I would upload shortly
very helpful for students before exams, hope to have many useful videos like this in the future.
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I'm not a biotech student bt ur explanation helps me alot .... thanks for the videos...keep going on 👍👍
Glad to hear that.....i just make videos in my spare time....you can support me by sharing my videos in your college group
You can also suggest topics for making videos on
I will try
@@animatedbiologywitharpan As this yr JAM biological sciences exam is discontinued....can you pls able to make video on Biotechnology jam syllabus
My channel has all the videos which could be good to iit jam biotechnology
Are yeast shuttle vector and yac same. If not what is the key difference? It would be of great help if u could help me with this doubt.
Wonderful explanation thanks a lot sir
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Very Useful! Thank you!
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quick checking so what are the vector that's shuttles and expression? can I said YAC is shuttle type and how about BAC and other plasmid like pSC101,pBR322 and pUC >"
Sir kindly reply why mrna itself cannot be ligated into cloning vector??
There are few reasons the vector back bone is double stranded while mRNA would be single stranded moreover it’s hard to isolate mRNA of a specific gene and mRNA is highly unstable
Please share my channel link in your college group and help me to reach big audience
Thanks..❤️
Please share my channel link with your friends and help me to reach big audience
👍 thank you
Please share my channel link with your friends and help me to reach big audience 😁
"save your ass the day before exam" he knows why we are here
Ha ha ha .....nice comment... anyway if you like the content please share with your friends and help me to reach big audience
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Me right now
So a shuttle vector are man made or synthetic? Plz answer I am very confused?
They are artificial vectors created by scientists
Thank you
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From where r u learning this concepts suggest me the books
these days i only read research papers but the books you need to read is given nicely in this videoruclips.net/video/z1xCAX4hrfE/видео.html
wh y is BAC (bacterial artificial chromosome) not a shuttle vector? pls answer
Miss AK it was not designed for that purpose.....key contribution of bac in biology was to make big genomic libraries....it was never used in gene expression purpose....all these vectors are designed for specific purpose and need so bac is not designed to shuttle between host
@@animatedbiologywitharpan you said that bac is not designed for that purpose then you said that it was designed to shuttle between hot (last line) I am confused
Sorry I was typing in hurry .......read the comment again
Is YAC a shuttle vector?
What is the difference between YAC and yeast episomal plasmid?
Rishima Borah the first yac to be described was designed as a shuttle vector
Yeast episomal plasmid or yep is also a shuttle vector with much lower carrying capacity than the yac...... actually I have a video on that I would upload shortly
@@animatedbiologywitharpan Yeah. Sure,Thanks!
Express in both bacteria and Yeast
I appriciate your efforts for being consistent.
Bacterial ori and Yeast ori😊
please share my videos with your class mates
Multiple clonning site
Really thanks for your support
Bro speak in Hindi naa
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