[How to Grow Mushrooms] Making a Grain Spawn Master using liquid culture and agar.
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- Опубликовано: 18 янв 2020
- How do you prepare grain for spawn? How do you make grain to innoculate with mushroom spawn? What is grain spawn and how is it used growing mushrooms? How to expand your mycelium? If you have any of these questions or more about how to make a master grain spawn that you can then expand into hundreds of pounds of mycelium.
Watch as Andrew goes over the technique discussing sanitization, what you need to do to be successful and what not to do! If you have any questions regarding anything within the video, make sure to leave us a question!
As always, don't forget to tell Ben he's awesome, great or fantastic!
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#keepspawningculture #grain #spawn #culture 
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![🍄Liquid Mushroom Culture [Agar Series - Episode 2]🍄 How to pour agar plates and slants](http://i.ytimg.com/vi/cpTmq66mTss/mqdefault.jpg)
![🍄Liquid Mushroom Culture [Agar Series - Episode 2]🍄 How to pour agar plates and slants](/img/tr.png)
I cant tell you how your videos inspire, I think I am ready to start my own production, thanks man
I love that feeling of the mycellium sucking up through the syringe. Beam me up!
Man thanks a lot for going through both ways of innoculation!
Always a joy watching you work!!
Thank you!
Thanks for all the great advice really enjoy the videos keep up the good work!
Another informative relaxed demo.
Thanks Man. 👍
Thanks! Glad you enjoyed it~
Thank you so very much for the visual instructions on this process. I appreciate you tons. 🤗
This guy is like a giant teddy bear mushroom grower
Thanks for this family.
Love your work
Thank you! We appreciate our followers!
Loving the GT oyster master jar 😁🍄
There is a legend passed down generation to generation that says the GT Oyster Mushroom got its name from an ancient Germanic culture that named them after “an educator whose worth is that of precious metals”. History is so fascinating.
Love the GT Oyster grain spawn, looks nice and clean
GODBLESS man I bought all I needed started my inoculations already. made a laminarhood
Awesome videos!! Thank you
can you please do a video about senescence, storage, and producing backups of your masters? Thanks for everything!
I've been viewing your videos lately and find lots of good info and tips. Thanks. Hope to do business with you in the near future.
I enjoy your vids! Something I haven't yet heard or read is how much water to start with in the pressure cooker, especially for the 2.5 hours you recommend.
Great question! Added to the list!
Jan 2020: "Rubbing alcohol is cheap"
June 2020: I can't find any rubbing alcohol in the tri-state region
lol. right? just buy a bottle of everclear and dilute it with distilled water. ethanol is literally better than iso in about every way.
@@TM-pi2rn Yeah, I'm using denatured ethanol (branded as fuel alcohol). It seems a little cleaner than most denatured alcohols. They put a sketchy ketone in there though, so I don't put it in a spray bottle like I would IPA. For any spray work, I'm using a 10% bleach solution in water. Been doing this for a few weeks now and no uptick in contamination (knock on wood)
Good job gimmlie. You put down the hammer and found a less violent hobby. Gandalf would be proud.
Bruh😂
Stop asking question he answered in the video. Watch at 1.5x speed if you don't have a lot of time.
Thank you for making growing mushrooms EASY .
If you think it's easy, you have not been at it for very long.
It's a good idea to have a plate over your bags so you don't block the pressure gauge or vent
Good Video!
After watching your videos my alcohol usage went up 100% and my contamination came down about the same 🤘🏽
Your hair on your bottom lip is amazing. It splits and goes side to side. Amazing.
Funny, I was noticing the long grey hair, pretty much the only ones.
Who gives af ?
And that's why I'm subbed or course
I love your videos, so helpful. Do you have a recommendation for a water to molasses ratio to propagate spores from a syringe? I think Stamets recommends 5 gallons (80 cups) to 50ml molasses for a slush, which I thought would probably work.
you material its nice sir please make a video for how you do for your special jar.thank you
How many lbs are the 3 bags you pulled out of your pc?
Btw- love your videos. They are super helpful. I really love the recap part you always have at the end
Thank you! Those three bags were 5 lbs each.
Nice soundtrack.
Hello my friend, your videos are great, I hope you always be successful, I have a question, I use food coloring in agar and this causes the leukoid culture to get a dark color and the mycelium in the liquid cannot be found properly. Do you use a colored plate in the leukoids? Thanks. I wish you success again
Great video can you tell me the best place to get those Air port lids
Just threw it up on Facebook to help out😁🍄🥦🥦🍄🍄🥦
Thanks!
Thanks for the very informative video!! I was wondering if you ever use corn, and whether you would do the same thing with corn (soak for 24 hours prior to sterilization)
Another great video. Wanted to ask - can you recommend any one type and strain over all others in your experience up to now? What grows most vigorously and trouble free, which is the most popular?
the King Blue Oyster is a brawler, and is my all time favorite to eat!
Great video as always! I suggest upgrading to a stainless steel bench - what material is the bench now? Is that grout in-between those hexagon tiles - keep up the great work guys MUSHLOVE
It's plywood with enamel.
@@Mossycreekmushroomsofficial cool way to customize the look while maintaining a smooth surface - I am a big fan of stainless because of the longevity but it is very boring in appearance
@@FreshfromtheFarmFungi Yeh, we actually messed up the process, but I'm liking the worn look to the table. Gives it a heavily used appearance. Kinda like "pre-worn" jeans. ;)
Thank you for the high-resolution videos! It allows us to see detail and the little things that make a difference.
Question, once a grain master is grown in how long is it viable? Do you refrigerate or keep it room temperature if you don't use all of it immediately?
When is the next Ben engineering do-it-yourself instructional video coming out? Got to love that ingenuity and get it done attitude :-)
@@blairdavison1274 I don't know when he is doing another one. He does them as he has time.
@@blairdavison1274 I don't like letting masters sit for long. You can keep it in the fridge for a good while, but the longer they sit the more likely they are to be contaminated later.
@@Mossycreekmushroomsofficial Sorry we were hunting specifically for this question! Is a while 1 week? or 1 month? 1 year? THANK YOU!!
I just use boiling water from kettle to add to LME to make my LC. I have never cooked them longer than 15 minutes with no contam issues. Maybe its the fact I start with boiling water.
That track knocks fam....where you get that from?
I would like you show or tell how to make powerful dish master mycelium ! Or give advice where to read more about that
quick question, after your agar have mycelium do you store them in fridge? also when you put agar on your grain bottle does the bottle need to be in dark warm place?
10cc for a small jar is insane Andrew! Nice video thou, as always.
Yeah that's a lot for one jar I have problems with to much moisture when I do that
It's a lot, but it's the fastest grow in I've discovered so far. The jars grow in super fast that way. If you go more than that though you might get slow growth in the bottom. LC is cheap, might as well get speed out of it.
@@Mossycreekmushroomsofficial I understand the reasoning. It being cheap. You get more surface... It's a matter of bulking lc which is easy. I retract my commentary sir. You are right!
Btw, started using oats because of you. Now I wonder why God even created other grains! :) But I use a different Tek. I believe it gives me better hydration. Cooking without soaking.
@@salvadorpalma8173 Oh, you don't need to retract anything. Everyone is doing it a bit different. Oats are amazing!
Hey Andrew is it just a light behind your LC on the stir plate just for ease to see what's inside
Have you done a vid where you show how you get from grain spawn into the masters mix? I've seen your machine create the master's mix bags, and the steamer you built, but not the final mixing together. I assume the master's bag is cut open, grain dumped in, and the bag resealed.
Will you PLEASE demonstrate the Ziploc Tek. Everyone speaks on it but I can not find a video.
which episode do you have the steam generator? I need to grab some info and ideas off that one. Thanks.
Very nice video thank you.
Can I use grain to grain transfer for again and again?
Yes, until it gets contaminated.
Hi idol bro watching from bikol PASACAO CAM Sur philippines
I noticed you mentioned incubation for the agar jar, do you need to incubate for the liquid culture jar and if so at what temp would you prefer
Very informative. Question though: Would you use old fruiting blocks to inoculate fresh grain spawn bags?
No, I wouldn't do that at all. I've tried it before, and it's very dirty.
Dear sir, how about liquid preparation can you instruct us.
So, after make the grain master, you transfer to growing bag or you made another "grain parents?" Cz here in Indonesia, the grain master we called as F1 then from the grain master, we transfer it to F2 which basically another "grain parents" after F2 we transfer to F3 or we called as growing bag
Make a vid on how to make that flow hood plz!!!
There are online intructions to similar flow hoods . Fairly simple to build.
Where do you store your liquid culture ? For future use?
Do the kids on the jars need to be tightened down or slightly loose for escaping gas?
How do I actually see how the fungi spawns around the oats you let dry how do they actually come about im trying to find out what to use for the mushrooms too spawn I want to usually intent to use achol and a hint of sugar for my spores to be injected into the bags
Great video thanks, the content was great but the music at the end capped it off.
Hey Andrew, great video 👍
I have seen a video in which a jar of liquid (prepared) was innoculated with a small peace of mycelium on agar from a pd.
What do you think of that?
I love it, I do it all the time!
I appreciate this, thank you. I notice you weren't flame sterilezing your needle at first from new to LC to grain jar. Have you had any problems with that?
He used brand new sterilized packaged needles.
Can you grow saffron milk cap ?
I'm still having trouble with the wet spot sour right so I'm going 90 minutes should I go to two hours or two and a half hours
Love the video, I aim to be like you. Could you give me any advice?
Watch all the videos and ask any questions you might have! We may not respond immediately, but you never know, we could just as easily feature it in a video! Thanks for checking us out, we hope you find what your looking for!
stay clear of Mossy Creek Mushrooms, it will rain isopropyl alcohol for 40 days & 40 nights LOL
(just being funny about the deluge of ISO you use)
I am making my 3rd round of watching every one of your videos in order of upload date.
Thanks I am very happy that I live very far above sea level :)
I have a question about the grain jar from agar dish, how is the lid made?
I saw the Synthetic Filter Disc - 90 mm at shroomsupply, but do you buy the lid pre-made with the two holes? because I didn't see it :/
Whats the point? Just take lc to a normal spawn bag and if you need to expand use that spawn to make more.
Great videos always Andrew! Let’s let one more myth go alright, scalpels are not intended to be flamed red hot. It just needs a couple quick passes. Yeah it may look cool but all it’s doing is damaging the blades sharpness and nothing else. Not a criticism, just your voice is being heard so spread the spores
Thank you for the suggestion. I appreciate filling in the holes in my knowledge.
@@Mossycreekmushroomsofficial Didn't you have a Bacti-cinerator Benchtop electric Sterilizer??
@@john-smith. I didn't, but I have one now.
Nice video! What was the discount code for shroom supply? Don't see it in the description.
Please use the code "Mossy"
Shroom Supply: 10% off your order
Thanks!
I see your pressure cooker in some of your videos, but is that what you use to sterilize all your substrate? You seem to have quite a few bags in your grow rooms. Do you use a bulk sterilzer? If so, what do you use. I haven't had any luck finding much without requesting quotes from commercial suppliers.
I only use the pressure cooker for culture work and grain spawn. I use an atmospheric steamer for my bulk substrate. You can see a glimpse of it in my fruiting block video.
why don't you color your liquid culture?
How many vials do you get from one of you pint jars
I kept thinking you had all purpose flour on your counter top.
Why do those jars leak so much?
hai man i need your help i am from india here every one is working old style and always depend on weather now i want to in house all setup as you do in my case volvariella volvacea locally call (paddy straw mushroom) if possible tips or one to one session
so cute
Be honest. Haven't you been briefly tempted to light the alcohol?
Might i suggest instead of isopropyl alcohol, that you use hospital grade sani-cloth. It's not flammable and kills everything very effectively.
I would hate to see you get that neat "blue glow" to ya ...
Do you have to make sure the blade is cool before cutting into the agar dish? Wouldn't the blade only kill the mycelium immediately next to it, or does putting a hot blade in the dish somehow kill a lot of the mycelium next to it?
The moisture cools the blade surface off pretty quick, but in most labs we press the blade flat against the edge of the agar to cool the blade before cutting. I don't know if it makes a big difference but that's what they teach people in universities/colleges. You can search microbiology lab culture techniques and find a bunch of really usefull general info about lab tech and procedures right here on youtube and google as well. Hope that helps.
Hi Andrew, have you ever considered injecting the LC into the substrate. Without the extra step of grainspawn. And if its a nutrient problem one could add rye into the substrate, and sterilize. what do you think about that?
Yes, I've had mixed results with it. I'm running more trials on it now.
The grain provides some nutrients, which is helpful if all your feeding the mycelium is carbon.
Thanks for the video. Not understanding why you make a grain master jar and then use that to make grain spawn? Why not just make bags of grain spawn and skip the master jar step?
To save spores and mycelium probably
Can u make a side by side only to see what its better and faster to make spawn,if the liquid cultures or the pitry diches???
I think this is an interesting idea!
I just did this. A full plate of agar; 2 ml, 4 ml, 10 ml, and 40 ml liquid culture.
2 ml was slowest
4 ml = agar (but nearly the same as 2 ml)
10 ml fastest (2 days faster)
40 ml not happy. Too wet and did not fill out the bottom of the jar.
Hey I’m just getting started out and want to try and make my own liquid culture. I was curious to know what size syringe and what gauge needle you used to get the tissue before putting it in the jar?
I have like to use a size 14g, especially when drawing up nice chunky mycelium
@@rubentorres4071I personally rock with 16g. Ive seen as small as 18g but I wouldnt want anything that small. 14g works great. I have a bunch laying around for those chunky bois.
How do you go about finding what strain of mushroom to grow and where to sell them? Especially now, with restaurants and farmers markets basically being shut down, I am afraid I will be investing money on fungi and have no one to sell to.
Think of the future, Michael. This pandemic wont last forever!
Hi Anthony may I ask where you get your alcohol because it is not cheap where I get it?
Amazon. 25$ a gal
How to prepare liquid culture ???
Can u tell me with exact measurement?
4-5% sugar in mineral water
Have you ever had issues with the bags sticking to the sides of the psi cooker??
It can if your pressure cooker runs out of water.
Can I use LC lids on grain jars and vice versa?
Sure.
Hey quick question. I noticed two of my agar plate samples have the slightest blue tint in a spot or two have you ever seen such a thing?Is it contaminated or does it happen because it doesnt seem to spread or anything
Most likely contaminated. Any mycelium growth
But the internet says.... that the syringe filters do not provide enough air exchange for grain. I had not read this until after I tried it. The 0.22um x 25mm PTFE syringe filters are working great for me... with both liquid culture and grain.
Lol, same here. It works great. I'm going to start doing it this way everytime.
Unfortunatelly I had bad experience with syringe filters...I don't know how but when I made like 5 jars 3 with polyfil and 2 with syringe filters I had very very slow growth in my syringe filter ones and when I opened them up it was definetly a carbon dioxid like smell from the jars.
I don't know why that happened to me. Maybe because these were from china and low quality. Since then I haven't tried.
Barna,
There are 4 different types of filter material in the discs. Some melt at pressure cooking temperatures. The PTFE (Teflon) filters survive pressure cooking. Do you know the material in the filters that didn’t work for you?
Can you explain the different parts of the ventilator on your desk?
it's not a ventilator, it's a laminar flowhood
basically, it moves clean air over your work area and ensures that it has almost no chance of atmospheric contamination
He does sort of explain it in at least one other video. Maybe the one on LCs . There are 2 pre-filter in the upper column and then a heppa filter where the air flows out. Also would need a good fan in the upper column to push air through.
Is it bad to mist my hepa filter with alcohol? Thank you
no, it will evaporate, do not soak it, just a light spray.
using a UVC light is better though
What would colonize faster: agar slices or a syringe full of liquid culture?
I would guess liquid culture, though Ive not used agar I do know LC goes pretty damn fast.
MY question is how do you keep it at 15psi for 2.5 hours? You have some type of system at that keeps it at 15? Mine will go up and down and i have to manually adjust
If u put the oven eye on med it should stay close...u can adjust slightly til it says put. But never leave ur pressure cooker unattended.
I let my 26Q presto go at full blast until it gets to 15p , then I turn it down to about 3/4, if it stays at 15 after 5 mins ,I lower down to 1/2 flame, if it stays I’ll lower it down to 1/4 . If it goes down below 15, then blast for a few mins then try again. I can usually get it down pretty low surprisingly. It must depend on what ya got in there, jars or bags or lcs/agar.. josh, this is an old video right? U said 2.5 hrs pc? I’ve been pretty darn good with 90 min after it gets upto 15? Nice agar shaking / distribution advice! Never heard anyone talk about that! How to keep it from sticking to the inside of the lid etc… love your tunes , give us yer playlist?!?! 😊😊
15psi for 2 1/2hrs, so where would the water level have to be inside the cooker to prevent complete evaporation? (I'm new to this stuff, sorry)
Up to the tops of the bottom bags.
Curious about how you don't contam with the beard? I swear I pop the lid on my monotub and my beard hair instantly falls off and contaminates. LOL
Alcohol
What are you using to spray things down with? The contents of your spray bottle? Thank you
Secret recipe 😂
It’s most likely alcohol tbh.
He said it was alcohol and cheap.
@@cg5071 70 percent
70 % isopropyl boyz or it won’t work or hydrogen peroxide 3% w solution
You pressure cook those mason jars with that lid setup, but also without tin foil on top?? I've never used those lid or that setup and is why I ask.
aluminum foil is only really necessary if youre only using micropore tape with punched holes in lids to prevent any water dripping off the inside of the lid of the PC on to the grain jars and into the grain
@@const3llations_370
Is there a educational video teaching or showing how to make liquid culture using the pre mix from Shroom Supply?
@@royalprince8858 I believe so! Maybe he didn't make it yet? Not too sure about that. If I can find it I'll post link here :)
@@const3llations_370
That would be nice, thank you very much.
Which one spreads the mycelium faster in the grains, the agar one or the liquid culture one ??
debatable, both great !
Hard to tell I’d say g2g is faster but if you had really strong genetics lc might win
Do the bags tolerate that long in the Pc? I’ve had a couple bags get holes or melt it seems when I go over 2 hours.
turn down your heat. it only needs to be about 175f
You can also use a cotton dish towel or pillow case to surround your bags so they don't touch the sides. Ive burnt a few bags that way.
Why is it necessary to break up the initial growth of the mycelia? I can see that after breaking it up the grain master is consumed quicker. Do the mycelia reunite before they produce a mushroom? Can you reuse those lids with the injector ports. Once you close the bags is there an air supply to the grain master? I saw on another video there looked like a filter on the side of the grow bags.
To make it run quicker. Also some varieties will start to fruit in the older section of mycelial growth while the jar is still growing in.
@@Mossycreekmushroomsofficial So if you keep breaking up the mycelium it won't form "fruit", I can understand that, but do all these pieces combine at some stage in the grow bags? I might not be asking the question right. I'm totally amazed at the size of the clusters you end up picking.
@@robertdeboer2053 You just break it up once, and one more time when you gonna transfer it out.
The reason he breaks it up is to speed up colonization.
@@john-smith. OK I understand that. I suppose if you had enough time the bags could be shaken several times to speed up "colonization". But I keep on thinking if it is anything like growing vegetables you wouldn't want to have too many individual plants in a set area. So do the mycelia rejoin to form a single fungus combining to produce fruit?
@@robertdeboer2053 Yes, they reconnect. You do the same with the bags.
Can we get a link to the bags you use?
I can't link it here, but you can check out our blog for our how to make fruiting blocks post.
dont you have to pastuerize the grains first by cooking on stovetop and then pressure cook it?
Absolutely not.
nah pressure cooking will sanitize them, pasteurization is a less effective method that people use for straw mostly, or wood chips and such for substrate.. this is for grain spawn, and the pressure cooker does just fine