How to Make a Spore Syringe - for Lion's Mane, Reishi or Oyster Mushrooms with Mossy Creek Mushrooms
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- Опубликовано: 27 авг 2024
- I've had a lot of questions on how to make spore syringes. It's actually incredibly easy to do, especially with aggressive species like Lions Mane, Reishi, or Oyster Mushrooms.
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A good man who knows his stuff, plays caustic, and shares invaluable information for the well being of others. You sir are an incredible lad and I truly love your content. Keep it up brother!
Is this actually true does this man play caustic
I do indeed play as Caustic. @@Purplebb676
dude is playing apex with his look alike gibby ... hahahaha big fan dude keep it up
That's actually Caustic, I look more like him anyways.
Take a sterilized lid from a clean Mason jar. Place cap on lid. Add one drop of rainwater. Cover lid with jar for 24 hrs. Carefully remove cap. Use lid to top a jar with water and corn syrup. Colonization!! After a week suck up with syringe.
You know what would be cool if you took your sticks after your dun collecting spores and stick them out side in the back yard near the edge of a deck for shade and see if they grow.
Loving that auto focus
I like the versatile simplicity.
Former research chemist here.
Just getting interested in the hobby... This is so cool. I've always considered myself something of a scientist, so this gets me really excited.
"I'm something of a scientist myself"
-Green Goblin
Seriously one day this channel is as we say in stocks, "going to the moon!!" Can't believe it's not there yet but love everything you provide the community!! Thanks bro!!
I've tried getting spore prints off of shittake and chantarelles and always get contamination. Next time I'll try the chopsticks thing. I love it.
Dude, absolutely blowing my mind. Found you tonight, looking to scale up my grow and you have given me shortcuts I didn't know from little tangents you go on. Epic content, huge thank you!!!!!!
Thankyou Brother
Regards from Fiji
Love the work you guys are doing, after learning about the health benefits of mushrooms i began growing pleurotis, hericium, lentinus/lentinula, agrocybe/clitocybe....... Depending on which academic ur talking to and the date of the description. So much with names is changing lately. I learned most of my basic lab work from your videos so i have to credit all ive done since to you. Im now preserving indiana wild species and also breeding my own mushrooms now using mon-mon and di-mon pairings. Thank you because of this video i learned to take cleaner prints about a year ago and my whole game changed. Again thank you
Correction, agrocybe was changed to cyclocybe
Caustic main here. The experiments begin. :)
An oldie but still relevant. Great video🙏🏽
I do like the no fuss method as there are fewer steps therefore fewer opportunities for contamination.
Good video and cool music. You have inspired me to grow other types of mushroom besides cubensis all the time haha.
So much information. You speak clearly and are really easy to understand. Wonderful video, thanks
Thank you!
APEX LEGENDS AND MUSHROOMS! Instant like and follow. Also liked those other videos about grow room, liquid culture, the agar series and spent blocks!
This stick between the mushroom and the foil is a really good idea, altough I will go for metal rods that can be disinfected well, to really rule out contamination.
Thank you sooooo much for sharing all your wealth of knowledge. You're making A LOT of karma points there 👍😊
Better idea than cooling your flamed scalpel with the water: wait for it to cool down to the point that it is no longer visibly red hot (only takes 3 or 5 seconds) and hit it with an alcohol swab for a few seconds. Then hit it with a fresh paper towel and she's ready. Then dip it in the water for the moisture if you need. This is the proper way to do it actually.
Awesome video btw! I'm gunna check out your channel.
I really like your use of the tubes. Good idea to keep stuff even cleaner
Thanks!
I'm anxious to see the cloning video. Thanks for all of your videos and the wonderful information you share in them.
I'll be working on it for sure!
I remember commenting about how wild mushrooms can grow in spite of billions different types of microscopic organisms. And that it may have to do with developed immunity, and how exposure can increase immunity of organisms. We know this because of how vaccines work, introduction of weakened strains of viruses. And the fact that kids that play outdoors are known to have stronger immunities than kids that don't. And culture immunity, example Europeans don't react as badly to pox viruses as African or native Americans due to the fact Europeans were exposed to it far longer.... But what's interesting is genetic diversity. It's noted that species with the greatest genetic diversity have the most immunity to not only pathogens but changing environments simply because they have a greater Gene variation to tap into. If one parent is white, the other black, your able to use genes from both parents and their ancestors exposure to protect urself than a child with both white parents. So I wonder if that's why cultivated mushrooms succumb to disease easier than wild mushrooms, their Gene pool will be much smaller. At zoos they have a trade program to ensure captive wild animals maintain as much genetic diversity as possible. I wonder if a trade program amongst mushrooms, mixing spores of same species from different locations and wild versions could strengthen the cultivation?
I routinely bring wild mushrooms into cultivation and they have the same problem. It's really about how fertile the substrate is. Our rich substrates that support high yields are way richer than mushrooms would typically grow on in the forest. Therefore speedier organisms like contaminates are able to colonize your substrate quicker than your chosen mushroom species.
Mossy Creek Mushrooms interesting, that makes sense.
Thanks Mossy. Keeping it straight foward an clear. Im glad i found you.Cheers, from Australia.
Cheers back to you!
I have been loving your channel, thank you. I have done strain isolation on petri dishes, and although it worked it was slow and tedious. This seems like a much quicker way at getting results.
Very interesting.. thank you.. going to give this a go.
Excellent video Andrew....and your singing is pretty good too..
Lol, thank you very much!
Great video bro
Thank you for the video!
Hi! I have a request. Would you consider making a video demonstrating how to install onto a bag a self-healing injection port with tape. You say in the video clip that it is easy, and now you have me intrigued. Keep up the amazing work!! Thank you!
Thank you for sharing your wisdom.
Any time!
Soild stuff I'll be trying it out very soon.
Keep up the good work mate from Liverpool UK. Want to follow your lead some day soon👍🏻😁
Thanks!
Nice video! Thanks for sharing!
Thanks for watching!
see I'm a novice. He's a professional. he just in passing mentioned that he pressure Cooks his grain jars for two and a half hours. I just need to hang out with this guy for a while just watch iwatches videos I need to watch more
Much appreciated Mossy! Thank you for your time making these informative vids!
I love this dude... Thanks for all the great work. big fan
Thank you for the very good videos!
Thank you for all the information that youve been putting out 👍👍
Thank you for watching!
谢谢您分享自己的经验。
When is the right time to get spores from Lion's Mane? Which structures am I looking for? The long "teeth"?
i've been watching your videos,,i have alot of knowledge from you,,thanks alot for sharing your videos,,im starting mushroom lab and i need to know more about mushroom..
Awesome video, very informative
Thanks man
you are very welcome.
Never give up. Namaste
Thank you, and back at you.
I so look forward to your videos! I don't know why you would skip the agar step where a person can at least try and visualize and isolate bacteria/mold/etc. Why jump straight to a bag? "incompatible strains" with lines of demarcation on the agar - in the same species? Is this possible? Might it be contams instead? I fruit the mushrooms in the sterile grow bag, rinse/wipe the bag super clean, open in a flowhood, stick a gill piece to a petri lid with petroleum jelly and place over the media filled petri dish, wait a few hours, and replace with a new lid. Voila, sterile spores. I then plate out what I think are sections/isolates visually on agar as they expand. If you haven't tried my "stick a sterile gill to a lid" trick, give it a whirl, and report back.
I've done that before and I do like it very much. Truth is, it's just so little labor to do it with the syringe and I'm getting good results from it so I tend to go with the lazy way there! Lol.
You are a genius. Thank you!
Epic mushrooms and plays apex legends !! Thanks for all the knowledge really appreciate it. Have been really able to step up my mushroom growing skill over the past 6 months of watching your channel so again thank you! :). Also you ever up for a round in apex let me know I'll send you my info lol :)
Yep, Apex is awesome! Send it on over to Andrew!
cool environment! and great info!
Thanks, Andrew!
You are very welcome! Thank you!
Cool brother man
Thank you.
Nice to see you! ! !
Glad to be seen.
Thank you for the awesome videos!
Great inspiring video's! Love them all. Also enormous respect for the way you have set up your company.
I just started growing oyster mushrooms in dec. 2018 and try to do this in the most professional way as possible.
It's great to see every fase of the proces to get to the fully grown mushrooms. I just love them.
Your video's are a great help!
Hope you will make some more.
Thanks and keep on talking :D
#Healthymushrooms4U Holland
Hallo landgenoot. Ik ben zelf een kleine twee jaar oesterzwammen en shiitake aan het kweken. Ik heb een steriele kast gebouwd waar ik de oesterzwammen kloon. Fascinerend om uit een klein stukje zo'n resultaat te krijgen. Je geeft aan zo professioneel mogelijk te kweken; je wilt ook aan horeca gaan leveren?
@@1972taco hallo Hollander,
Inderdaad heel fascinerend te zien hoe uit zulke kleine stukjes zulke prachtige zwammen kunnen groeien. Ben nu idd ook bezig met Shiitake te gaan telen. Zit nu nog in de fase van uitzetten in graan en van daaruit verder. Ben alleen nog op zoek naar de juiste samenstelling van het groeiblok.
Ik lever mondjesmaat aan restaurants.
Deze video's zijn heel waardevol om kennis te verzamelen. Zeker als ze zo open en eerlijk worden gepresenteerd.
Thank you very much, and I will for sure keep making more!
In this jar of LC there is water and what also?
Love your videos, you've got some good methods worked out.
Thank you.
Thank you.
thank you for watching.
The cheapest way to make an injection port is just to buy a tube of high temp (red) silicone adhesive and squirt a glob on the outside of the bag lol
Loved the vid.
Btw, how long does the watery concoction last? If on a fridge, any chances it'd go for 3 months or more?
What to do if you want to keep a strain for while other than grow it?
How long do dry spores last?
Thanks!
Thank you for your mushroom wisdom
Thank you for watching!
Wow 👌👌👌👌👌
Great video. I notice in the no fuss method you appear to be using a hepa filter negative air system. Is that to avoid spores flying around? How much danger is there? What were to happen if mushroom spores flew around a room? Thanks
I love the tips and tricks you show
thank you very much for sharing knowledge 🙏🏿🙏🏿🍄👌🏿💯
Ayyyye I see you’re an apex fan 👍🏻👍🏻
Loved video didn't know you played Apex lol
I love it! Caustic all the way! My gamertag on Xbox live is mycominer.
Wow.. Awesome... Can this be done with shittaki?
How do you clone mushrooms? You mentioned it in the video
We have other videos please check them out!
How many time that the spawn will devasted the area of Allum paper and became White pouder? Thank you to sharing your knowlege
Do you inject lots of spores to speed up colonization, and production of mushrooms tolerating mild contamination because the tissue sample from the centre of the mushroom is pure? Or do you throw away grain that has contaminants?
Thumb up!
I just wanted to thank you for the informative video, so many that are giving good info for first time growers are by and for people growing P mushrooms. I am sure I am on a list now thanks to that... I do have one question, even though I see lots of people doing it with no issue, I opted for a inductive heater to heat up my instruments, is that better, worse, or the same as actual flame sterilization?
How do you avoid contamination in the spore print? I don’t see any sterile environment at this stage.
Instead of pressure cooking your grain, can you just boil it? I don’t understand the difference
How about contam from the mushroom, foil or air?
Is there any noticeable differene between the two methods?
can anyome point me to the part with the lions mane? i seem to be missing it constantly
So, your finished spore syringes aren’t sterile? You never did anything to kill contamination on the foil and spores themselves.
This is probably dangerous for new growers.. they grow some random mushroom in their batch and they’ll be like oh cool! Eat it and get poisoned...
Yep, You most certainly need to know what mushroom you are taking from.
Great video! Working on a wine cap bed in FL. I’m not a mycologist or biologist, but could I (in theory) potentially find a recessive gene for a heat-tolerant strain? There is a FL Blue Oyster strain on the market.
What are the risks of not sterilizing?
How long you leave the water for
Thanks 😊
I bought some lions mane mushroom and it's vacuum-packed and frozen. There is a white substance over the mushrooms, I was curious as to what this substance could be?
Literally looks like aerobic organisms are living on the bottom rim of your """sterile""" flow hood. 'Mycologist' has got the be the funniest people to watch :) Cheers and bravo, keep going lolz
3:24
oh God no.... 4:08 Bahahaha take that thing apart and clean it guy
What are they eating on is my question... supposed sterile gas? Why are they aggregating there? hmm
Do you have to inject it into a bag? Can you just inject it in small doses all over a mass of substrate?
Could I use this to inoculate a liquid culture jar?
You can, but spore prints run a higher risk of contamination.
What solution did you do ??
Hi Andrew, can I use the same method with Agaricus Bisporus? (button mushroom)
How much weight of grain is in per bag?
what are the specs on your syringes and needles? Size, gage and any other details I would need to start making my own LC syringes.
What contients the LC water and what substance also with ? Thank you for all the informations you sharing for us
Good day sir. What chemical inject to substrait to a spawn.
What chemical inject to substrait to forming become to spawn
Is that just straight plain tap water? (Yes, I know it was pressure cooked) or is there sugar or something else in the tap water?
👍
I can say I tried this method a few years ago and got nothing, not a single spore got to go ,I’m skeptical about these methods unless someone can show me this works
Which method? The first one or second one? Both? when I first started I inoculated Spore syringes I purchased online straight into my grain spawn, in incubation for a while inside a plastic tub on my floor every single one exploded with life and quite a few were contaminated. Did so many times over. But I never made my own spore syringes. Try buying one!
@@alekswhy88…so I’m doing things a bit different now , giving it another shot , my inexperience I think and lack of knowledge was my downfall , mossy grows shrooms so not blaming anyone just could not get mine working , btw the variety helps as I used button spores which is not ideal for the method I think , I’m doing oysters now and got the mycelium growing well , learners are bound to fuck up lol my bad. 😁
how many times can you clone a mushroom before you're just barely scraping the bottom of the gene pool with it?
Is it faster to grow the mushrooms right off the grain spawn than transferring it to the master's mix? If then, how faster is the prior method?
I'm not sure I entirely understand your question. Which prior method?
Is growing gray dove Oyster mushrooms the same as growing lion's mane mushrooms?
WAIT so you inject it directly into the growing medium? I'm so confused
👆👆For your psychedelic product 🍄
Look Him up 👆
Hey what's the song playing at the end
Take It by Killrude.
@13:26