I just complete my first grow of B+ thanks to your broke boi tek guide! I am totally obsessed with mycology and I appreciate all of your videos! This hobby is a gift that just keeps giving!
How are the B+ doing for you? I just ordered some prints and have heard great things. Only have experienced the Tidal Wave and Golden Teachers to date. Both great!
@@masonreed5684 it doesn’t matter which variety you grow of Cubensis. The process is exactly the same…exactly! A cube is a cube. The only difference are PE’s, and that’s when you spawn them to bulk because you need to case them or you get blobs and mutations. But the LC and agar process is the same. PE (penis envy) take longer to colonize as well.
Hi Teach, I just wanted to say thank you for your videos. I successfully harvested 870 wet grams of mushrooms on my first grow from your videos. :) I now have decided to take my grows out of the closet and into the entire spare bedroom. I ordered most of a mushroom lab and I am looking forward to grow #2 and then further agar plate/slant work and liquid culture production :)
@@commiecrusher I have tried them, probably before you was born. Not that it matters. My experience wasn't amazing was more on the nauseating side. Now I just take chaga, lions mane and reishi. I'm currently trying to grow cordiceps.
this is EXACTLY the tut I needed. I just did my own agars and previous went spore p to full fruit. you are doing gods work, and apparently we're on the same cycle.
The Presto PC is nice man. I want to thank you for all the tuts and all the knowledge. I’ve been doing this for about a year now and I’ve made quite a few adjustments based on your teachings. I strictly only grow APE, PE uncut and golden teacher now. Not a day goes by where I’m not working on Mycology. 🙏
I have the 28 qt Presto and It works very well. I just started doing LC's and for 1 or 2 quart jars, I use the instapot. Wife is not happy about that though. I'm going to start using 1/2 pint wide mouth jars for LC's rather than Qts.
Thank you sir, and everyone else out there for sharing tips, tricks and knowledge. In a lot of ways it's similar to the 3d printer circles. Great thing about having so many teks and personal tweaks (for all skill levels) is that you can "Chinese Menu" stuff. A 5 gallon bucket for cultivation, someone else's personal substrate mix, and little add on/tweaks/personal touches from 3 different people.
Nice one PGT! I prefer to use Li (liquid innoculant) for anyone thats interested it is sterilised water and mycelium (no nutrients) straight into grain. I do the step you do at 8:55 with the agar plate and go straight to grain, i find tjis method excellent. I never thought about sucking up the mycelium directly from the agar dish until i watched this so thank you for that. I love your videos PGT ✌️
@@sheeeitmayn4384 well surprisingly. I made two different LC''s B+ and Pink Buffalo. The PB spores LC I pulled them into 10cc syringes and I had about the same amount of contaminated jars as I had just using spores. That's using an SAB. BUT.... I just did up some of the B+ using same exact method and so far I'm almost to 100% colonized in my grain spawn and so far I have zero contamination. Only thing I did different was instead of inoculating jars in my kitchen I did it in the bedroom. And TBH I thought it would be a disaster because I keep reptiles, and I have had issues with cobweb mold before I got into growing mushies. But everything is going good so far. I did do a serious deep clean though because the web mold is super annoying just in snake cages. Especially in mine because they basically are perfect environments for growing mold. I don't use glass tanks. They're a humidity nightmare if you want it be consistent. Also I'm still working on probably going to end up building my own flow hood. Still doing the proper research on how a proper one is built. I almost went full on with that myco willy dudes build and learned that he doesn't mention a pre filter and I'm told this could mean the difference in getting months vs years from a HEPA filter. And right now we all need to save as much money as we possibly can.
@@ChondroMan so you had some luck using a spore syringe straight to liquid culture? Im at the moment going to agar and brown rice flower jars with my spore syringes but was wondering if i could shoot some into LC jar and it work out fine??
Great video! You having the agar open while you scrape is keeping it open and exposed while cutting a chunk of agar and dropping it in keeps exposure to a minimum.
Started 2 GT and 2 B+ dec 9 and 4 more of all PE the 22nd. One gt and one b+ didn't even spawn. Other two gonna go to bulk in a day or two. This my first ever grow. Also got agar plates and a microscope with plenty of slides. Following a lot of RUclips videos and I'm having an absolute blast. Thanks PGT.
Anything stopping you from poking the syringe through the agar cup's lid and doing the scrape-and-suck procedure, to minimize possible contamination time from the open lid?
@@chrishudson4940 always works quite well for me and the spores seem to enjoy themselves. Plus I get a visual indicator when all the suagrs are consumed as the fluid goes from a golden hue to clear
@@funkymonk4688 so total noob ?, i just got spores syringes. would it be best to make LC now, or just wait and take baby steps w just spores into grain and or all in 1 bag? And possibly wait for some "experience" before jumping that far ahead? And thanks for the info im still in planning stages
@@chrishudson4940 I im always a big advocate for baby steps. Especially since once you get your first flush you can make a spore print and make an LC from there. Take your time, do your reaserch and everything will be fine!! Best of luck! Plus golden teacher gives great advice!! Can't go wrong listening to this man
Thank you very informative lesson.. I made the mistake of using spores and it held up for about 3 weeks, then I dumped it into one of my spent cake flower pots outside and it ruined that too. Thanks PGT
Crackin eggs of wisdom. Lol. Man I need a new gasket for my pressure cooker and some spores or LC from a strain I haven’t done before and I’ll get to growing again. Right now my outdoor cyanescens patches are keeping me pretty excited. One of the patches has hundreds and I think will put up a thousand mushrooms this year. It’s so damn cool
Easy way to measure your 4-5% of sugar nums is to just use a syringe. 300 ml mark on a regular pint size canning jar and so a 12ml syringe of sugar nums will do.
Man just starting out in home mycology so glad I found your channel sir 🙌🙏👏 Thank you for sharing this information with the community, and fantastic presentation no nonsense consise and clear couldn't be better, I've got some watching to do now lol lots 😀
The reason I asked because I have an agar slant of wine cap dried up in the fridge for 9-10 years. I tried several attempts recusing the culture and finally have it on a single clean agar ketchup cup. The rests are not growing, contaminated, partially grow/partially infected. I used it to multiply on mor agar, all fail but one second ketchup cup. I do not want to repeat the process again but go straight to LC. I am afraid to fail and lost them all. 😢Thank you.
I wanted to take a minute to thank you for all the effort you put into creating your videos. As a (former) producer of educational content, I appreciate the time and attention to detail that goes into the pre and post efforts on videos like these. The prep, planning. editing cuts, audio, etc takes time and is very different from what most people experience when watching talking-head videos. Thanks a ton for your contributions to this community, you have helped many, many people improve the quality of their lives in profound ways. Few people fall into that category. Mush Appreciation.
Thanks for your fantastic videos. Never grown mushrooms and always wanted to learn. Thank you for explaining everything so simply that a noob can understand.
This timing couldn't have been better! Watching my slow mss inoculated grain jars grow...and wanting to learn more to speed stuff up😂 got some agar supplies in the mail now...but researching LC now too...so happy to see this video today from PGT! THANKS MAN!
I just ordered some premade agar plates as my plan was to go from spore syringe to LC. I just started learning with oyster mushrooms and didn't have to worry about that step. I'll head over to your vids on that now lol thanks for the info
Thank you for helping me along this learning journey! I did go from Spore syringe to Liquid Culture, and so far, no signs of contamination. I don't have anything inside the jar to help with the stirring so not sure if that will hamper growth or trying to extract any LC since it's not being broken up for easier getting.
After you have removed mycelium from the agar dish, do you use the same agar dish to populate more liquid culture jars until no visible mycelium are present, or is it best to discard that agar dish because it's been exposed to air? Thank you for your amazing contributions to our knowledge.
Sensei! I love you videos! I recently started using your recipe with peptone and using Boomer shroomers method of inoculation (taking part of the fruit through a syringe and injecting it in) and every jar I’ve had is cloudy. While I’m sure most of the problem is my sterile technique, I was wondering if you or anyone else has anyone tried this method of inoculation with this recipe? Is there a reason you inoculate from agar only? Thank you so much!
Let’s say I got LC syringe from a PGT recommended supplier, can this used to inoculate or does it need to go to agar to be screened for contam? Seems like they would have followed a similar process and it already be clean?
You can expand LC to LC. It is always best to test things or start from a clean sample off agar. But to answer your question, yes, you can use it to inoculate 🙏
Man I've been watching your videos for days. I have made so many mistakes but you seem to have a video for every question or mistake I've made. Plus covering so many diffrent teks!!!. Thx, your videos are a huge help!! P.S. I can't wait to eat some homegrown portobello and shittakki mushrooms!
So.. dont do spores>agar>liquid culture/slurry>grow? What would the better option be? Inoculating several agar plates from a single syringe>isolating clean growth> isolating aggressive mycelium>liquid cluture/slurry>grow?
You mention loosening the lids before you pressurecook them, do you tighten them after taking the lid off the pressure cooker (Once you've let them cool down?) I'm Using your video as a guide to grow Yellow Oyster mushrooms.
Sensei, I have searched all through RUclips and the internet but can't seem to find the answer, to what I think, should be easy to find. The question is: After storing liquid culture in the fridge, does it need to be back at room temperature before injecting it into grain jars for colonization?
Love your informative videos! Pssssssst - FYI PGT Yikes!! @ 9:19 you stuck your 18g hypo needle into your HEPA filter creating a hole of appx. one thousand six hundred and fifty microns... that filter would fail certification. You might want to squirt some silicone caulk in and around that hole.
@@harleyjackson4208 It did penetrate but the reality is you can't even touch that extremely fragile filter media with something sharp, specially a hypodermic needle without compromising the filter integrity as HEPA filters are tested to
Hi Sensei, I have watched all of your video's and was wondering can you just make a clone through LC instead of going through agar first. Wouldn't it be the same process? Of course it would have to be done in sterile conditions. Thanks for your time, take care and be safe.
@@ozoneswiftak yes, took 6 different samples placed 3 into LC. Did all work in my SAB and apparently 2 got contam somehow. 1 jar is chugging along. Put the other 3 samples to agar, working on 3 plate to get the best sample to send to LC. Hope this helped.
I got a 23 quart pressure cooker but it’s really old and I can’t find a new gasket for the model number I got. The ones I bought were just a little too big. Might have to just buy a new pressure cooker Got this one from my parents and it has got to be over 40 years old
Thank you so much for a simple but effective recipe for expanding LC. The recipe I've been using with Soy Peptone just doesn't work for the Black Pearl King Oysters. :)
Can I do the same with pepton , light malt extract, corn syrup to scrape off the mycelium off the agar? Also can I instead puncture the ketchup cup instead of removing the lid completely & squirting in the mixture in and scrape off and suck back out into the broth ?
It is possible but not ideal. Spores are inherently dirty with bacteria and potentially mold spores from exposure to open air. Ideally you want to clean up a culture on agar to use
I'm debating what's a better method? The 250 degree oven or the SAB. I feel like the oven is better. I'm not going to be dropping the coin for a flow hood any time soon! One day maby. Btw love that you reply to comments man, I'm shire I'm not the only one who greatly appreciates it!!!!
PGT, I had some bad luck with grains that were not sterilised long enough, they all colonised with LC but with contam. This time I did sterilise everything right, used LC from the fridge (had been standing there for a 6 to 7 weeks or so)... nothing is happening unfortunately.. 2 weeks in.
Maybe the mycelium was damaged too much as I shook the jars streight after inoculation. Some jars I did not shake (like the unmodified lids as there is a higher chance for contamn) and those do grow a few tiny bits of mycelium.. so it should still be alive somewhat
Konnichiwa Sensei, excellent! Now maybe I can get a successful LC. Q: Does powder dextrose work? how much for 500ML? You mentioned LME. How much? How does the LME aid in LC?
Can we just put distilled water in the syringe and put it inthe agar and scrape those off and re-suck those into the syringe for a one time use instead? If I don't wanna a lot of it in a jar.
Have you used the latest corn syrup craze? It's got vanilla salt And ethyl alcohol. Will that crap also work? Can't find old fashion corn syrup anywhere besides online
You can also just keep the agar in a sterile jar with an injection port. Take from the un-inoculated culture and mix it with the agar, then just transfer it back to the liquid culture jar though a injection port.
@@PhillyGoldenTeacher but what if he needs liquid culture for innoculation of cakes? Because you can't innoculate cake with grain, right? I'm total noob, not sure
Sorry if this is a dumb question, I'm just learning. After you have the LC colonized do you add that to your sub for fruiting? I've never tried this. I always use rye berries to colonize. Thanks! Love your videos... so much great info! ✌🏼
i think a lq is still for inoculation of grains. This is just the absolute fastest way i think. (im also new so maybe someone more experienced can better answer)
hey philly would you do a video on wedge agar to lc and also I know this might not be possible but a video on cleaning up a contam agar I know you kinda did this with your agar tek video but id like to see how you handle a fully colonized agar with some green mold on the side or something around that.
PGT is single handedly colonizing mushroom growers. Status ... legend
😄
I just complete my first grow of B+ thanks to your broke boi tek guide! I am totally obsessed with mycology and I appreciate all of your videos! This hobby is a gift that just keeps giving!
How are the B+ doing for you? I just ordered some prints and have heard great things. Only have experienced the Tidal Wave and Golden Teachers to date. Both great!
@@TopherHess1974 B+ is nice. But I prefer the teachers. F+ is really nice as well
@ intellectual crackhead I am bout to grow some b+ aswell I have a good syringe of them. Did the video method work good for b+?
@@masonreed5684 it doesn’t matter which variety you grow of Cubensis. The process is exactly the same…exactly! A cube is a cube. The only difference are PE’s, and that’s when you spawn them to bulk because you need to case them or you get blobs and mutations. But the LC and agar process is the same. PE (penis envy) take longer to colonize as well.
B+ were my first grow! Super fun and yields great 👍
I make my own maple syrup, I use that in my agar and liquid culture. They seem to love it and stays very clear.
Tell me u r Canadian without telling me u r Canadian: 😂
Organik bal da olur mu?
@@hrg06 You should probably use pasteurised honey instead. Less chance to be contaminated.
Hi Teach, I just wanted to say thank you for your videos. I successfully harvested 870 wet grams of mushrooms on my first grow from your videos. :) I now have decided to take my grows out of the closet and into the entire spare bedroom. I ordered most of a mushroom lab and I am looking forward to grow #2 and then further agar plate/slant work and liquid culture production :)
@09Kromerman could you explain why people should experience psilocybin?
@@ranjanty it's amazing. Try it and find out for yourself!!
@@commiecrusher I have tried them, probably before you was born. Not that it matters. My experience wasn't amazing was more on the nauseating side. Now I just take chaga, lions mane and reishi. I'm currently trying to grow cordiceps.
I wish people would just say the amount in dry weight, I know wet is a bigger number but it's less accurate since water content varies.
@@selfactualizer2099 87dg
Just tried LC using his technique. I am tickled pink it is 3 days old and showing lots of life. THANKS Teach.
this is EXACTLY the tut I needed. I just did my own agars and previous went spore p to full fruit. you are doing gods work, and apparently we're on the same cycle.
hes the goat
I’ve had mycelium grow on top of my LC very strong strains 😎🍄❤️
damn i keep failing growing green mould
how do i get my own agar dish?
@@joshturner9766 Stick to the tek. And take every percaution to the extreme. It will work.
The Presto PC is nice man. I want to thank you for all the tuts and all the knowledge. I’ve been doing this for about a year now and I’ve made quite a few adjustments based on your teachings. I strictly only grow APE, PE uncut and golden teacher now. Not a day goes by where I’m not working on Mycology. 🙏
I have the 28 qt Presto and It works very well. I just started doing LC's and for 1 or 2 quart jars, I use the instapot. Wife is not happy about that though. I'm going to start using 1/2 pint wide mouth jars for LC's rather than Qts.
Thank you sir, and everyone else out there for sharing tips, tricks and knowledge. In a lot of ways it's similar to the 3d printer circles. Great thing about having so many teks and personal tweaks (for all skill levels) is that you can "Chinese Menu" stuff. A 5 gallon bucket for cultivation, someone else's personal substrate mix, and little add on/tweaks/personal touches from 3 different people.
Funnily enough, printers are great for mixology too ;)
Nice one PGT! I prefer to use Li (liquid innoculant) for anyone thats interested it is sterilised water and mycelium (no nutrients) straight into grain.
I do the step you do at 8:55 with the agar plate and go straight to grain, i find tjis method excellent.
I never thought about sucking up the mycelium directly from the agar dish until i watched this so thank you for that.
I love your videos PGT ✌️
Late reply, but how do you find that method working for you? Going from the agar cup with the LC liquid right into grains?
Well this was the LC video I needed. You're spot on with the info. I'm also regretting using spores for my first LC's. Oh well. You live you learn.
Same!
Just put some lc on agar and isolate the clean mycelium takes extra time but beats contam right?
@@sheeeitmayn4384 well surprisingly. I made two different LC''s B+ and Pink Buffalo. The PB spores LC I pulled them into 10cc syringes and I had about the same amount of contaminated jars as I had just using spores. That's using an SAB.
BUT.... I just did up some of the B+ using same exact method and so far I'm almost to 100% colonized in my grain spawn and so far I have zero contamination. Only thing I did different was instead of inoculating jars in my kitchen I did it in the bedroom. And TBH I thought it would be a disaster because I keep reptiles, and I have had issues with cobweb mold before I got into growing mushies. But everything is going good so far. I did do a serious deep clean though because the web mold is super annoying just in snake cages. Especially in mine because they basically are perfect environments for growing mold. I don't use glass tanks. They're a humidity nightmare if you want it be consistent.
Also I'm still working on probably going to end up building my own flow hood. Still doing the proper research on how a proper one is built. I almost went full on with that myco willy dudes build and learned that he doesn't mention a pre filter and I'm told this could mean the difference in getting months vs years from a HEPA filter. And right now we all need to save as much money as we possibly can.
@@ChondroMan so you had some luck using a spore syringe straight to liquid culture? Im at the moment going to agar and brown rice flower jars with my spore syringes but was wondering if i could shoot some into LC jar and it work out fine??
We love you PGT❤️you have been guiding my journey through the thick and thin!!!
Great video! You having the agar open while you scrape is keeping it open and exposed while cutting a chunk of agar and dropping it in keeps exposure to a minimum.
It has been to long since you have posted a new video. Thank you again for your hardwork!
Class is in session. Finally it’s here Couldn’t have came at a better time was thinking about starting some!!! Good looking out bro bro. 🔥🙏🙏🙏
One of my favorite channels.
Thanks for the info, and keep fighting the good fight.
This was a clear and concise video and I really appreciate it.
Started 2 GT and 2 B+ dec 9 and 4 more of all PE the 22nd. One gt and one b+ didn't even spawn. Other two gonna go to bulk in a day or two. This my first ever grow. Also got agar plates and a microscope with plenty of slides. Following a lot of RUclips videos and I'm having an absolute blast. Thanks PGT.
Sounds like you got many fun things to start with! Wish you the best on your journey, you got this! 😁🙏
@@PhillyGoldenTeacher ty sir, can u do a video on genetics or genetic manipulation, I'd like to make my own strains.
@@PhillyGoldenTeacher
Total Novice trying to avoid early mistakes. Seeking suggestions for Syringe distributors. Any recommendations?
Anything stopping you from poking the syringe through the agar cup's lid and doing the scrape-and-suck procedure, to minimize possible contamination time from the open lid?
I always have used honey for mine, just inoculated some mckennaii's. Can't wait for them to be done!
me too love, the macs
And howd it go
@@chrishudson4940 always works quite well for me and the spores seem to enjoy themselves. Plus I get a visual indicator when all the suagrs are consumed as the fluid goes from a golden hue to clear
@@funkymonk4688 so total noob ?, i just got spores syringes. would it be best to make LC now, or just wait and take baby steps w just spores into grain and or all in 1 bag? And possibly wait for some "experience" before jumping that far ahead? And thanks for the info im still in planning stages
@@chrishudson4940 I im always a big advocate for baby steps. Especially since once you get your first flush you can make a spore print and make an LC from there. Take your time, do your reaserch and everything will be fine!! Best of luck! Plus golden teacher gives great advice!! Can't go wrong listening to this man
Not only the nicest voice of all myco-teachers, also just super funny...absolutly LOVE the little deer!! Got it as a tatto on my rist..
Waiting on pgt to make videos on isolating genetics before I start trying. Your videos are way easier to understand and keep it simple
Isn’t he basically saying grow it, pick the healthiest fruit, clone them on agar, and use that genetics?
Oh yes. And teaching us what each of the terms mean when looking at mycelium types and breeding mushrooms
Really excellent video PGT! Love you brother; really awesome to see you bringing the gold again.
Thank you very informative lesson.. I made the mistake of using spores and it held up for about 3 weeks, then I dumped it into one of my spent cake flower pots outside and it ruined that too. Thanks PGT
Crackin eggs of wisdom. Lol. Man I need a new gasket for my pressure cooker and some spores or LC from a strain I haven’t done before and I’ll get to growing again. Right now my outdoor cyanescens patches are keeping me pretty excited. One of the patches has hundreds and I think will put up a thousand mushrooms this year. It’s so damn cool
And thank you so much for all your videos! Almost ready to start my journey into mycology thanks to you!!!
Easy way to measure your 4-5% of sugar nums is to just use a syringe. 300 ml mark on a regular pint size canning jar and so a 12ml syringe of sugar nums will do.
Man just starting out in home mycology so glad I found your channel sir 🙌🙏👏
Thank you for sharing this information with the community, and fantastic presentation no nonsense consise and clear couldn't be better, I've got some watching to do now lol lots 😀
The reason I asked because I have an agar slant of wine cap dried up in the fridge for 9-10 years. I tried several attempts recusing the culture and finally have it on a single clean agar ketchup cup. The rests are not growing, contaminated, partially grow/partially infected. I used it to multiply on mor agar, all fail but one second ketchup cup. I do not want to repeat the process again but go straight to LC. I am afraid to fail and lost them all. 😢Thank you.
OMG it’s here!!!
I wanted to take a minute to thank you for all the effort you put into creating your videos. As a (former) producer of educational content, I appreciate the time and attention to detail that goes into the pre and post efforts on videos like these. The prep, planning. editing cuts, audio, etc takes time and is very different from what most people experience when watching talking-head videos. Thanks a ton for your contributions to this community, you have helped many, many people improve the quality of their lives in profound ways. Few people fall into that category. Mush Appreciation.
Love your videos. Simple, easy, loaded with information. Thank you.
Thanks for your fantastic videos. Never grown mushrooms and always wanted to learn. Thank you for explaining everything so simply that a noob can understand.
This timing couldn't have been better! Watching my slow mss inoculated grain jars grow...and wanting to learn more to speed stuff up😂 got some agar supplies in the mail now...but researching LC now too...so happy to see this video today from PGT! THANKS MAN!
I just ordered some premade agar plates as my plan was to go from spore syringe to LC. I just started learning with oyster mushrooms and didn't have to worry about that step. I'll head over to your vids on that now lol thanks for the info
Good luck on your journey! You got this! 😁
omg PGT the marbles in the jar are freaking brilliant
I'm starting my fungi journey next week and your videos have taught me the ins and outs of fungi
I'm glad the videos were helpful! Wish you the best on your journey. You got this! 😁🍄
Thank you @PhillyGoldenTeacher, as always you make mycology easy and fun. Cant wait to make some cultures.
I wish you the best on your journey! 😁🍄
Very informative in-depth information.
Thank you for all the videos, I am just getting started into mycology and can’t wait to do more studies.
I wish you the best on your journey! 🙏🍄
Out here crackin' eggs of wisdom.
Thank you for helping me along this learning journey! I did go from Spore syringe to Liquid Culture, and so far, no signs of contamination. I don't have anything inside the jar to help with the stirring so not sure if that will hamper growth or trying to extract any LC since it's not being broken up for easier getting.
Drag the needle through the glob you want to pull and then suck up what you broke off. And this is what took to the millet the fastest.
Really late to this comment but you could sterilize a stir bar or marble and then plop it in the jar carefully in a sanitized environment.
Watching it a 2nd time already. Thank you PGT, amazing quality videos everytime.
After you have removed mycelium from the agar dish, do you use the same agar dish to populate more liquid culture jars until no visible mycelium are present, or is it best to discard that agar dish because it's been exposed to air? Thank you for your amazing contributions to our knowledge.
Sensei! I love you videos! I recently started using your recipe with peptone and using Boomer shroomers method of inoculation (taking part of the fruit through a syringe and injecting it in) and every jar I’ve had is cloudy. While I’m sure most of the problem is my sterile technique, I was wondering if you or anyone else has anyone tried this method of inoculation with this recipe? Is there a reason you inoculate from agar only? Thank you so much!
I know what I'll be doing next weekend!! Love the nice clean look. Keep it up man!!
I set that reminder, my boy 🤙🏾🍄✨
Made an LC the other day had some minor sediment so had to redo that bad boy. Keep it up 👍
With LC y'all need to try adding one gram of PEPTONE to any LC recipe? Makes super LC.
Let’s say I got LC syringe from a PGT recommended supplier, can this used to inoculate or does it need to go to agar to be screened for contam? Seems like they would have followed a similar process and it already be clean?
You can expand LC to LC. It is always best to test things or start from a clean sample off agar. But to answer your question, yes, you can use it to inoculate 🙏
Does isopropyl alcohol also kill the mycelium that you're trying to grow? This question has been bugging me for weeks as I'm a beginner
yes it does, but it isn't like you never use alcohol directly to it
If you spray alcohol directly on. It will harm and bruise mycelium.
Knew I saw this in one of your fine videos. Thank you, should have saved it the first time. You rock.
After I inoculate my liquid culture jar with MSS, how long should I wait to start using stir plate?
The artwork consistently tickles my fancy! Do you sell prints of the sketches?
Man I've been watching your videos for days. I have made so many mistakes but you seem to have a video for every question or mistake I've made. Plus covering so many diffrent teks!!!. Thx, your videos are a huge help!! P.S. I can't wait to eat some homegrown portobello and shittakki mushrooms!
I'm glad the videos help! It's fun to learn, you'll get better and better, and we all make mistakes! Wish you the best on your journey! 😁
So.. dont do spores>agar>liquid culture/slurry>grow?
What would the better option be?
Inoculating several agar plates from a single syringe>isolating clean growth> isolating aggressive mycelium>liquid cluture/slurry>grow?
At 9:20 you put a hole in your flow hood filter with the needle.
Omg!!
The Safety is #1 priority guy!!!!!
I forgot about him. I’m looking him up now! Super 😎
You mention loosening the lids before you pressurecook them, do you tighten them after taking the lid off the pressure cooker (Once you've let them cool down?) I'm Using your video as a guide to grow Yellow Oyster mushrooms.
Thanks for simplifying this with the measurements mL/gram conversion🤘
Man this is a great video, made it very clear and simple!! Thank you!!
🍄
Gosh... you're a legend mate. After first harvest... u just mist the tub? That's it!?
Keep it up!
Really informative. Well delivered. Thank you sir 🙏
From Newcastle, Australia
Best videos on the web on this subject
Sensei, I have searched all through RUclips and the internet but can't seem to find the answer, to what I think, should be easy to find. The question is: After storing liquid culture in the fridge, does it need to be back at room temperature before injecting it into grain jars for colonization?
(no expert, but yes, bring to room temp before)
Love your informative videos!
Pssssssst - FYI PGT Yikes!! @ 9:19 you stuck your 18g hypo needle into your HEPA filter creating a hole of appx. one thousand six hundred and fifty microns... that filter would fail certification.
You might want to squirt some silicone caulk in and around that hole.
Watched it several times. I don't think it penetrated.
@@harleyjackson4208 It did penetrate but the reality is you can't even touch that extremely fragile filter media with something sharp, specially a hypodermic needle without compromising the filter integrity as HEPA filters are tested to
Great video. You have helped me so much. Thank you
Thank you very much
It’s very helpful and explains very clearly step by step
I’ll start my own liquid culture soon
I'm glad the video was able to help! Wish you the best on your journey! 🙏
Hi Sensei, I have watched all of your video's and was wondering can you just make a clone through LC instead of going through agar first. Wouldn't it be the same process? Of course it would have to be done in sterile conditions. Thanks for your time, take care and be safe.
Did you try this ?
@@ozoneswiftak yes, took 6 different samples placed 3 into LC. Did all work in my SAB and apparently 2 got contam somehow. 1 jar is chugging along. Put the other 3 samples to agar, working on 3 plate to get the best sample to send to LC. Hope this helped.
As always, thank you PGT! Is it possible to make a LC jar from a LC syringe? Or with a colonized grain transfer?
Did you try this?
Yes. It works. @@ozoneswiftak
You said you would add in light malt extract as well but i was wondering how much you would add
Yes! Love your stuff man!!
I got a 23 quart pressure cooker but it’s really old and I can’t find a new gasket for the model number I got. The ones I bought were just a little too big. Might have to just buy a new pressure cooker Got this one from my parents and it has got to be over 40 years old
Thank you so much for a simple but effective recipe for expanding LC. The recipe I've been using with Soy Peptone just doesn't work for the Black Pearl King Oysters. :)
Thank you, PGT!! Please keep up the stellar work.🙏🏼
🙏🍄
Can you please make a video on how to make lc syringes?
Can I do the same with pepton , light malt extract, corn syrup to scrape off the mycelium off the agar? Also can I instead puncture the ketchup cup instead of removing the lid completely & squirting in the mixture in and scrape off and suck back out into the broth ?
Hi and thank you for the great video. Could you tell me how long mycelium on the average will survive on an agar plate in the refrigerator.
When starting form a clean spore syringe. How do you select for sertain genetics? What do you look out for when selecting?
Hey, i'm currios, can you inoculate your jar with spores? just like the mycelium just scraping the spores from the sporeprint.
It is possible but not ideal. Spores are inherently dirty with bacteria and potentially mold spores from exposure to open air. Ideally you want to clean up a culture on agar to use
I learn so much from you. Thank You. 👍
Thank you for being a part of this journey! 🙏
I'm debating what's a better method? The 250 degree oven or the SAB. I feel like the oven is better. I'm not going to be dropping the coin for a flow hood any time soon! One day maby. Btw love that you reply to comments man, I'm shire I'm not the only one who greatly appreciates it!!!!
The oven can be easier to work with because it is open to moving freely. But the SAB is better practice and suggested! 🙏
"I'm cracking eggs of wisdom"
stole my comment
@@rightnearthebeach is it really stealing if he thought of it a day before you??
@@justincash1573 yes, hater because my comment was before the video even premiered.
@@rightnearthebeach *yes hater,
Hahahahahah dying 😅🤣😂😹😆
PGT, I had some bad luck with grains that were not sterilised long enough, they all colonised with LC but with contam. This time I did sterilise everything right, used LC from the fridge (had been standing there for a 6 to 7 weeks or so)... nothing is happening unfortunately.. 2 weeks in.
Maybe the mycelium was damaged too much as I shook the jars streight after inoculation. Some jars I did not shake (like the unmodified lids as there is a higher chance for contamn) and those do grow a few tiny bits of mycelium.. so it should still be alive somewhat
Make sure you have proper gas exchange, otherwise it will stall things out.
Awesome!
I just started my first 2 liquid cultures yesterday. Now I'm curious to see, if i did right =)
Thanks for another very helpful tutorial, PGT!
😄
Konnichiwa Sensei, excellent! Now maybe I can get a successful LC. Q: Does powder dextrose work? how much for 500ML? You mentioned LME. How much? How does the LME aid in LC?
Lme aids in providing nutrient. About 1.2-1.5g in 500ml solution.
Thank you I think this was the best video ive seen yet that answered all my Questions, I will name my 1st mushroom after you lol😬
If you are using agar you want to grow the agar out for 2 weeks to make sure that there is no contamination
alternatively you could just add powdered charcoal or antibiotics to forget about contams altogether
Can we just put distilled water in the syringe and put it inthe agar and scrape those off and re-suck those into the syringe for a one time use instead? If I don't wanna a lot of it in a jar.
Have you used the latest corn syrup craze? It's got vanilla salt And ethyl alcohol.
Will that crap also work? Can't find old fashion corn syrup anywhere besides online
ahh yeahh missed ya broo mush love!
As always. You are the GOAT
You can also just keep the agar in a sterile jar with an injection port. Take from the un-inoculated culture and mix it with the agar, then just transfer it back to the liquid culture jar though a injection port.
Do you need a pressure cooking to sterilize the liquid culture solution?
No pressure cooker. What do you think about boiling water/steam?
You can use a steambath to sterilize lc
What are your thoughts on squirting water in a jar of rice culture and sucking it back out if you don’t have agar?
It's risky. But not impossible. I personally wouldn't do it. You might as well just do grain 2 grain transfers at that point.
@@PhillyGoldenTeacher but what if he needs liquid culture for innoculation of cakes? Because you can't innoculate cake with grain, right? I'm total noob, not sure
Hi Teacher I have a question. Why do spores "mate" on a substrate or in a nutricious Environment but do not in a spore solution you can buy?
Because spore solution contains only spores + distilled water, nothing nutritional.
Sorry if this is a dumb question, I'm just learning. After you have the LC colonized do you add that to your sub for fruiting? I've never tried this. I always use rye berries to colonize. Thanks! Love your videos... so much great info! ✌🏼
i think a lq is still for inoculation of grains. This is just the absolute fastest way i think. (im also new so maybe someone more experienced can better answer)
Howd it go so far
Nice Lonza ad haha I used to work there 😂
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@@PhillyGoldenTeacher making 3,600 ml of LC as we speak brotha 😜 I sincerely appreciate the help you’ve given me over the past year fam 🤙🏽
Can we use potato tea to make liquid culture in addition to the sugar ingredient added?
hey philly would you do a video on wedge agar to lc and also I know this might not be possible but a video on cleaning up a contam agar I know you kinda did this with your agar tek video but id like to see how you handle a fully colonized agar with some green mold on the side or something around that.
THE best channel on the Tube