How To Turn 1cc Of Liquid Culture Syringe Into Unlimited Spawn.
HTML-код
- Опубликовано: 29 фев 2024
- This video teaches you turn 1cc of liquid culture OR spore syringe into unlimited amounts of spawn. Cultures can be stored for up to 12 months this way before its time for a refresh of stock.
You will learn how to store, manage and maintain a liquid culture syringe properly at home.
_______________________________________________________________________________
Further reading on the topic can be found here:
=================================================================
Maintaining LC Syringes, some extra tips in here.
ezmushroom.com/grow/how-to-ma...
=================================================================
LME Liquid culture recipe.
ezmushroom.com/grow/light-mal...
=================================================================
LME agar recipe.
ezmushroom.com/grow/light-mal....
================================================================= 
Хобби
🍄Buying Liquid Culture? Tinctures or Mushroom Powders In The UK? 🍄
🍄Checkout EzMushroom.com/shop🍄
🚀Use Coupon YT15 for 15% off🚀
Well done, man. Keeping everything as clean as possible is always a good thing. Mush love!
A couple of notes for those in the comment section.
I would only use about half of the malt extract suggested. The amount being used in the video is what I would use for an entire liter pour.
I would suggest against the use of red coloring. *Some* red dyes inhibit fungal growth.
Note to the content creator
I love that youre using Broke Boi jars
Have you ever considered getting yourself one of those continuous mist spray bottles for your isopropyl? They work amazingly and use even less liquid.
I would also suggest 20mm self healing injection ports for your LC jars. They are super cheap and keep you from having to open your LC jars to draw into a syringe.
Nicely done video, and good camera angles. Keep up the good work 😉
That looks awesome, ive been thinking about doing agar but put it off due to complication but that looks super doable. I am aware of agar plates having a small gap for air exchange and one of your plastic tubs has an air filter made from micropore tape, would you recommend that ?
Where did you purchase your still air box ? Great videos I’ve just came across your channel and I have subbed. Thanks for your time and effort 👍🏻
We sell them on our store, however we are out of stock right now, you can get notified when back in stock
ezmushroom.com/shop/mycology-equipment/still-air-box-mycology-mushroom-cultivation/
NorthSpore have a similar product, and there are some on amazon.
i got one on temu for 15£ works great
So i get this, master syringe > agar plates > ( grain jars / liquid culture), liquid culture > grain jars. then repeat process to make more liquid culture. So few questions, why not directly master syringe to liquid culture skipping agar plates ? also how do you refresh the master syringe ? also wanted to appreciate the video, always thought flow hoods are must to attain perfection, glad to see it can be achieved using SAB.
You get to test your syringe for contamination on agar and will know pretty immediately if you failed (within a weekish). If you see contamination you can transfer clean Myc and clean it up.
And because you are using a SAB its a good idea to confirm you have a clean culture before going to liquid culture and you start questioning sterile technique instead of a dud syringe or culture.
For refreshing, move it to agar then a new LC nutrient source and see if it refreshes. However best thing to do is take a piece of myc and put it in longterm storage on agar / distilled water, sealed. I haven't got a video on this currently but search Edward Grands video as he discusses it and so do others.
Tobh LC can still be contaminated even after a successful agar run. You could have likely just got the bit of LC out that wasn't affected. I mean you could just save agar slants just as long so idk. Everyone has a method that works and I say use it.
Firstly, nice video 👍
However I'm not getting the step of going to agar first. Put 1ml to 400ml of LC. Then grow that up. Then test your new LC on agar to check for contamination. Then to be absolutely sure when to going to grain, give the LC a good mix and then inject your grain AND onto some agar to check for contamination if you need to. If this method works for you then keep going but it's an extra step that's not needed in my opinion.
@@glyndwrevans I disagree vehemently with your assertion that the initial test is unnecessary. You should always test the LC you receive, first, before using it to do anything.
Once it tests clean, only THEN should you expand it to properly sterilized LC broth or inoculate grains with it.
Nice video. Is that a Feather #7 scalpel handle? I wish I could buy those here :) I have to use the shitty Hilbro ones. LMFAO
Indeed it is, feels great to work with, slim.
I've been watching your videos on serial dilution all weekend man so its great to hear from you, big fan haha!
Planning on using it for cordyceps
Check out the 'grab and drag' stuff. Way easier. I haven't done a serial dilution in over a year. @@ezmushroom
Dude where do you get those agar pots from?
What’s the best temperature for growing mycelium in liquid culture and how long until you get a full colony in a 600ml jar ? Thanks
74-80f is ideal. If you stir it daily, 10 days should be good, then refrigerate it if you plan on trying to elongate its viability. If you plan on using it all up in the first 6mo or so its fine to leave at room temperature
Did you have any excess water when cooling down the agar ?
Yes there will be excess inside the container but this clears up after a few days.
Could you give me the link to those cute little petri dishes that can be sterilized? Thanks.
amzn.to/45bjuYN
@@ezmushroom Thank you so much!
Do you have a link for those reusable agar pots.
I just picked those up from Asda in the UK.
Thanks, i picked up similar from Aldi, the pots and lids were polyethylene and not polypropylene, they were crushed by the sterilisation process. Lesson learned.
@@ianwynne5483 you can use condiments cups (ketchup cups) from the local box stores just pour them in the SAB. They work fine and maybe have 3 out of 40 or so get contaminated. (Only once was there three usually none when I'm sterile)
I can't understand what you said that you "plopped" into the LC jar to stir around?
It was a magnetic stir bar.
amzn.to/3X4lYWM
Thank you.
Do you have case of contamination throughout the whole process? Even if you are vert precotionous i wanna Know if contamination is common even if im very careful with sterility
I didnt get any contamination. If you follow all the steps in this video you should be fine. If anything goes wrong just try again until you get it right its daunting at first but gets easier as with anything.👍
Professional growers and companies consider 10% contamination rate acceptable and good enough to make profit. So if you're 10% or less you're doing good
Where do you buy the jars?
I don't buy Jars. Buy Beetroot, Pickled food, Saukraut, etc. I've collected most my Jars over years. The lids are often interchangeable and if they break they were going in the bin anyway so nothing is lost.
@@ezmushroom Thank you! Can I use a couple of drops into the agar, once the agar is colonized, could I do agar to agar?
@@goazu Yes definitely.
I'm surprised that heat sterilizing the needle doesn't kill the mycelium inside it.
I would assume the spores inside the metal needle would cook but all the other spores in the syringe should be fine
This is correct^
Only a tiny amount will be inside the needle which gets pushed out
Do I need a “stirring plate”?
No it just speeds up the process
Thank you for your prompt reply. How long does it take for this process without the stirring plate and do I physically shake the container?
Probably 5 days faster. My advice is just to give it a shake it daily, lightly. You dont want the LC going over the lid and possible seeping out etc.
Alternatively you can leave it to just clump, then when you go to draw it up, pump the syringe which will break it up.
Its probably worth me doing a video outlying this soon as I never used to use a stir plate.
Ill put it in backlog👍🏻
i cant understand english. i feel really dumb or i need to sleep.. the most sterile is the outside edge or the center of the agar
Take samples between the center and the edge.
@@ezmushroom ohhhhh so never go too close to the edge or the center where you droped it... that means i get the growing ends of the hyphae genius