Nice tutorial i like the recipe, just a few comments on your other processes. Why did you inject an entire 10cc spore syringe its a waste of spores you could have added a few drops, and did you qc the syringe on agar before to make sure its not contaminated? Also at the end you ruin the sterility of your LC when you add it to the syringe in a few ways. Firstly, assuming you autoclaved the LC syringe, you negated that by placing it on non sterile surfaces, then spraying it with a sanitizer(spraying iso on a sterile object makes it sanitary and no longer sterile, 2 very different things). Second when you pull the sanitizer into the syringe you make the inside of the syringe no longer sterile. Lastly when you run the needle through the micropore tape you expose a large hole to open air then put your fingers all over it. Not trying to hate or anything a lot of people dont have the air quality to work like this without contaminations is all. Here's what I'd do differently: prep: Autoclave the LC syringe in tin foil and keep it wrapped until ready. Use a RTV injection port (you can stab those thick blunt tip LC needles thru it many times) process: Operating in a flow hood or still air box. Prepare by exposing the connection end of the syringe and place it down not touching any surfaces. Open the syringe and slide it half way out of the package, remove cap inside sterile package. Remove syringe completely and attach it to sterile needle by holding the needle from the tin foil making sure never to touch it. You can flame sterilize the needle but its not necessary if you pressure cooked it well. Wipe and spray 70% iso on RTV port. Put syringe in RTV injection port and suck up ur LC. When you finish pull the needle out(the jar remains sterile). Remove needle, you can touch it now. Put syringe in sterile package and secure the cap inside of it without ever touching it.
I wish I could pin this to the top! Great advice, and I hope more people can see it in the comments. This will definitely help folks have a lower contamination rate. With the technique (or lack thereof) I'm using, I've been experiencing pretty low contamination rate, ~15%. That's not ideal, but it's working great for me and I'm okay with losing a jar every now and again. Appreciate your response and advice!
@@OneEarthMushrooms 15% is decent but if u lived in some of the houses i've lived in it would be up to like 80% it really depends on ur environment and a lot of other factors like if outdoor molds are sporulating that season and stuff, im glad its working for you but im sure you could get ur contam rates close to 0% if you practiced a bit more sterile technique. I like to watch biochem lab demonstration videos on sterile technique as they've been doing it for hundreds of years in that field
@@MycoDogs I appreciate the time you've taken to provide us all with some additional Qualty Control processes. I could see where if I were just providing my own culture just for me the extra labor and processing time doesn't sound high value for a 15% failure rate on a easily scaled system. I would definitely be following your recommendations at any point where other people are being provided that LC or consistency in my results are mission critical. In any case I will be taking your recommendation and looking into sterile lab techniques mostly out of curiosity but also to have that information available in my pocket. 👍👍Thanks.
3:00 Candymaker has a tip for you: Sugar starts to crystalize if you only use one type of sugar with water and heat - no matter which sugar you use (glucose, sucrose, fructose). Corn syrup contains only glucose sugar (usually something like 30% of weight is glucose while the other is water). If you mix glucose with sucrose (in your case something like 1-2g sucrose into the 12g syrup as around 3-4g of the syrup is glucose), it will not crystalize and you have not to worry about it.
@@metarox69 It comes to the molecular level of the sugar. As sugar (fructose, glucose, sucrose, lactose, maltose...) is a crystal molecule, and it is mixed with only the same kind, then it will be able to bond and create crystals. But as fructose and glucose for example are different molecules, they will not be able to form a bond with each other, and therefore no crystallization. When you heat sugar, it vibrates and breaks out of the regular crystal form. Mixing in another different type of sugar molecule and they will mix together as any liquid will. When the mixture loses heat, the crystallization begins to form. If you cool down the mixture very quickly, the molecules will be mixed together and they do not have enough time to separate. Therefore crystals will not form. But if the mixture is slowly cooled down (over hours or days), the molecules will have time to find each other. The molecules are attracted to the same kind and will find their way to form a strong crystal bond. The longer it takes for the mixture to cool, the bigger the crystals will become, because the more molecules there are to attract to each other (this is how rock-candy is made for example). There is an article where the writer explains this with good illustrations. You can find it by searching "the sweet science of candymaking" by "Tom Husband", published in the website "ACS". I hope this explained roughly how the sugars work.
@@danielalt7508 Very nice to know the answer but is not usable within homemade mycology tools because almost everyone use glass a not pyrex(borosilicate glass) which is very expensive so cooling down quickly will result in breaking the regular glass container especialy in a pressure cooker when you cant release the pressure very fast for the risk of contamination and the sudden PSI drop can break the glass too
@@metarox69 Very nice insights. Also I just notice I wrote a little bit wrong on the previous message. Paragraph 3 is not meant to refer to a mixture of different sugars, but instead to a mixture of water and one sugar... I must have written that in confusion on the usage of the word "mixture". Basically having 2 different sugars in one mixture, will never form crystals until a very long time has passed and the mixture is left untouched (for example honey that goes from liquid to solid). The rock candy is just one type of sugar (usually sucrose) mixed with water, and then cooled over long period of time. This means that having a sugar combination of 2 different sugar types in an autoclave is not going to crystallize. -------------- If I remember correctly, the mycelium absorbes easily monosaccharides (just like humans), which are fructose, glucose and galactose. The ideal sugar mixture would be a combination of at least 2 of these sugars for the mycelium, and mixing those will prevent crystallization.
I got a 10cc syringe from a reputable dealer. I have used a grand total of 4cc from the syringe and in return I have 10 flourishing, clean agar plates and 3 quarts of popcorn inoculated. The remaining 6cc in the syringe is so dense with spores it's amazing. The agar plates are for isolating strong specimens, then the LC comes from my favorite from that. For a $21 syringe, you can do a WHOLE LOT. For sheer ghetto points, I have never even screwed the provided syringe on. I have used 4cc of spores completely bareback from the lurelock port. I observe reasonable aseptic techniques but do not have a laminar flow system. None of the 10 agar plates are contaminated.
Psychedelics definitely have potential to deal with health issues like anxiety and depression, I would like to try them but it's just hard to source out here.
Yes the easiest way to get you a contaminated culture. Goof luck with the lack of knowledge. Never just watch ONE video and think you "got it down". You will waste time and money watching videos from amateur growers like this.
Give yourself another gift: If you tab the tape on the roll when you're done, it makes it easier to pull for next time, plus you already have your tab ready to go! Great videos and love your calm energy, thank you!
You are a rare breed on RUclips, sir. Your videos are direct, not gimmicky, easy to follow, and delivered in a professional manner. Thank you for your efforts. One question. What gauge is the blunt 4 inch needle?
What a great pedagogue you are. Can't stop watching your videos. Little question here. Where (i guess fridge) and how long does your liquid culture keep?
Thank you, Nathalie! Correct, the fridge is where I keep the culture. I've kept them for up to 6 months in the fridge, though they may last longer. Six months is the arbitrary cutoff I've given myself to make sure they are viable when I inoculate.
My question is, if in 6 months I take one of the syringes and repeat the process, will I ever need to buy any of that strain ever again? Should be repeatable forever, correct?
@@FantomFirez Multispore Syringe to Liquid Culture will take 10-21 days. Agar to Liquid Culture or Liquid Culture to Liquid Culture will take as soon as 3-5 days. Multispore Syringes have to germinate first. Then after germination, they have to find other nearby spores through sending hyphal threads outwards in search. Multispore Syringes as a result take 2 - 3 times as long to colonize than any other method. And your Multispore Syringe/the spore print that was used to create said Multispore Syringe could have had contamination that you will not have noticed until very far later and very much further down the pipeline of growing, nearing fruiting conditions or right after spawning to bulk substrate.
Whilst viewing a different video, it was recommended to use a magnetic shaker and magnetic 'pill' every day and not to leave it for a week before checking on anything. Any thoughts?
Just wanted to add on the contamination worries especially for us first rate newbies,.. if its too overwhelming to make mason jar blenders and opening jars etc,.. make the liquid substrate jar with an injection port,.. and inject bought liquid syringes. This is not to knock the poster’s skills,.. i just find this video very advanced compared to where i am at this point in time. Also i live in a 200 year old house from hell (building a new place thankfully) but theres every possible way to contaminate in this house i am in right now. It would increase my chances of success if i bought a pre done spring and injected it into a sterile jar of liquid culture. I can learn to make that jar, to add nutrient and inject sterile air etc. but making the Petri dish, cutting it etc thats a ton of opportunity to add contaminants when done by me😊.
Yes, definitely can use honey. I haven't seen much difference other than the honey cultures seem to have more sediment, which makes it more difficult for me to tell if there is contamination. But, it's always worked fine for me when I've used honey! Thank you for the kind words!
Great video. Thank you for your information. So now you have 10 30cc syringe where you drew mycelium (not spores in through a 12ga needle. When you are ready to dispense into spawn jars or bag,, do you have to use same sized 12ga needle? Or a more standard sized injection needle?
Thanks for this awesome video, I gotta question, can I use Honey instead of kernal syrup ? If not, what else can I use in case I dont find kernel syrup in my country please
In order to get mycelium growing the quickest… is it faster to turn spore syringes into LC rather than just inoculating my grain with the spore syringe?
Awesome video dude! Question, I want to do this but syringe is a live culture syringe with mycelium in it instead of spores. Can I do the same process and just inject the jar with the mycelium I already have instead of spores?
The first thing that comes to mind is using an instant pot, but I don't think that's the essence of your question... I'll have to do some thinking on this, I'm sure there's another way, but everything coming to mind involves heat sterilization.
Take a pot and put jar rings on the bottom fill with ur jars and then add water to the bottom of the jars. Cover and bring to a boil then put it on a simmer for 60-90mins then let cool
Look up broke Boi tek and he has videos on no pressure cooker sanitized jars. I do it. Works great. Just let it stay in long enough. Also make sure ur jar lids are lose so the glass doesn't crack
I’m confused. Did you not chance contamination with the spoon and syringe by pulling liquid into and out of that same syringe when you added nutrients ? Or was that off camera ?
Nice hair. So do you see contamination in the LC or do you just roll the dice? Is it safe to assume that the same thing can be done from LC to LC? How manc CC of LC would you add to 800ml of solution?
Hi there, I have a question: Is there any other sweet nutrient to replace the corn syrup or does the nutrient have to be exactly corn syrup? Thanks for your advice!
THIS IS ONE THE BEST CHANNEL I HAVE COME IN CONTACT , PLEASE TELL US THE NUTRIENT YOUR USING . IS IT LME PDA SYRUP HONEY PEPTONE??? REPLY BEFORE I MAKE MISTAKE THANKS AND REAMEN BLESSED
So? Mycelium can be broken up and reform, kinda like a sponge? (Sea Sponge can be divided into indiv. cells, and reform) I thought if I broke it up too much I'd be killing some, or at least slowing it down.
Great tutorial as always. I am wondering how many, lets say 1 liter grain jars (filled 3/4th) can you inoculate with a 30 cc syringe? or is it a syringe per jar.
For a 1 pint jar of grain, use 1-3cc or 1-3ml of the syringe. So technically a 30cc/30ml syringe can inoculate 30 different jars if you wanted to do that. Also store the LC in fridge when you're done and it will last 6 months to up to like 2 years if my memory serves me well
@@DamnBrother how much fruiting substrate do you use per pint jar/pound of grain spawn? New to this trying to figure out how much of each thing to get 😂
@@oliviafinnegan2437 everyone has different recipes but mine I use 200 grams vermiculite, 200g coco coir and 40g of gypsum. I weigh this all out dry and pasteurize with about 8 cups of water. This is enough substrate to use in a 33qt tub, and I use 1-2 pint jars of grain spawn. 2 is sort of overkill but hey it's just me.
Hi Kayla! An instant pot will work well too. I have on my list of experiments: "microwave sterilization of liquid culture" but I haven't gotten there yet.
GOOD LUCK TO YOUR WORK SIR, AM ABOUT TO START NOW , THE NUTRIENT YOUR USING IS IT LMA OR AGGA AGA. NUTRIENT AGGA ? PLEASE REPLY BEFORE I MIX . THANKS AND REAMEN BLESSED
Going to be doing this today in an instant pot. Do you put the jar right on the bottom of the cooker or do you put the insert in so the jar is not directly in the water ?
I've got a question, Would this work by using a 💉 of liquid culture? Or does it have to be done with spores only? Also, I wish you sold liquid culture on your site, I'm in the market for some as I am about to do some research pretty soon. ❤
I had an issue or problem that maybe someone has suggestions for. I had two setups, identical to yours in a pressure cooker, and water boiling at 20 psi (vs 15) caused rock to jump around tapping the bottom of the jar and splitting the bottom off both 312mg setups. Just like tapping glass for a controlled break the bottom was 360 tapped and broke. Any suggestions other than not have a rock in there? Great sterile technique. My water level was a little deeper than yours and just touched the glass bottom
Great video, mate. So simply communicated. I live In the UK and Corn syrups are called high fructose corn syrups and isn't easy to find due to production quotas. Are there alternatives?
It shouldn't since you're pasteurizing the water in the pressure cooker, but with all the potential crap in city tap water (lead, microplastics, etc.), wouldn't distilled be the better choice?
Does anyone know a substitute for corn syrup? For some reason I can't find any in my area. Also how long to keep the jar before extraction of the mycelium after all the process shown in the video?
![Felix "Unfair" | [Stray Kids : SKZ-PLAYER]](http://i.ytimg.com/vi/Oswujxm2Ag0/mqdefault.jpg)
Nice tutorial i like the recipe, just a few comments on your other processes. Why did you inject an entire 10cc spore syringe its a waste of spores you could have added a few drops, and did you qc the syringe on agar before to make sure its not contaminated? Also at the end you ruin the sterility of your LC when you add it to the syringe in a few ways. Firstly, assuming you autoclaved the LC syringe, you negated that by placing it on non sterile surfaces, then spraying it with a sanitizer(spraying iso on a sterile object makes it sanitary and no longer sterile, 2 very different things). Second when you pull the sanitizer into the syringe you make the inside of the syringe no longer sterile. Lastly when you run the needle through the micropore tape you expose a large hole to open air then put your fingers all over it. Not trying to hate or anything a lot of people dont have the air quality to work like this without contaminations is all.
Here's what I'd do differently:
prep:
Autoclave the LC syringe in tin foil and keep it wrapped until ready.
Use a RTV injection port (you can stab those thick blunt tip LC needles thru it many times)
process:
Operating in a flow hood or still air box. Prepare by exposing the connection end of the syringe and place it down not touching any surfaces. Open the syringe and slide it half way out of the package, remove cap inside sterile package. Remove syringe completely and attach it to sterile needle by holding the needle from the tin foil making sure never to touch it. You can flame sterilize the needle but its not necessary if you pressure cooked it well. Wipe and spray 70% iso on RTV port. Put syringe in RTV injection port and suck up ur LC. When you finish pull the needle out(the jar remains sterile). Remove needle, you can touch it now. Put syringe in sterile package and secure the cap inside of it without ever touching it.
I had the same response
I wish I could pin this to the top! Great advice, and I hope more people can see it in the comments. This will definitely help folks have a lower contamination rate.
With the technique (or lack thereof) I'm using, I've been experiencing pretty low contamination rate, ~15%. That's not ideal, but it's working great for me and I'm okay with losing a jar every now and again.
Appreciate your response and advice!
Update: I figured out how to pin your post!
@@OneEarthMushrooms 15% is decent but if u lived in some of the houses i've lived in it would be up to like 80% it really depends on ur environment and a lot of other factors like if outdoor molds are sporulating that season and stuff, im glad its working for you but im sure you could get ur contam rates close to 0% if you practiced a bit more sterile technique. I like to watch biochem lab demonstration videos on sterile technique as they've been doing it for hundreds of years in that field
@@MycoDogs I appreciate the time you've taken to provide us all with some additional Qualty Control processes.
I could see where if I were just providing my own culture just for me the extra labor and processing time doesn't sound high value for a 15% failure rate on a easily scaled system.
I would definitely be following your recommendations at any point where other people are being provided that LC or consistency in my results are mission critical.
In any case I will be taking your recommendation and looking into sterile lab techniques mostly out of curiosity but also to have that information available in my pocket.
👍👍Thanks.
3:00 Candymaker has a tip for you: Sugar starts to crystalize if you only use one type of sugar with water and heat - no matter which sugar you use (glucose, sucrose, fructose). Corn syrup contains only glucose sugar (usually something like 30% of weight is glucose while the other is water). If you mix glucose with sucrose (in your case something like 1-2g sucrose into the 12g syrup as around 3-4g of the syrup is glucose), it will not crystalize and you have not to worry about it.
what is the fundemantals behind this?, i am very curious
@@metarox69 It comes to the molecular level of the sugar. As sugar (fructose, glucose, sucrose, lactose, maltose...) is a crystal molecule, and it is mixed with only the same kind, then it will be able to bond and create crystals. But as fructose and glucose for example are different molecules, they will not be able to form a bond with each other, and therefore no crystallization.
When you heat sugar, it vibrates and breaks out of the regular crystal form. Mixing in another different type of sugar molecule and they will mix together as any liquid will. When the mixture loses heat, the crystallization begins to form.
If you cool down the mixture very quickly, the molecules will be mixed together and they do not have enough time to separate. Therefore crystals will not form. But if the mixture is slowly cooled down (over hours or days), the molecules will have time to find each other. The molecules are attracted to the same kind and will find their way to form a strong crystal bond. The longer it takes for the mixture to cool, the bigger the crystals will become, because the more molecules there are to attract to each other (this is how rock-candy is made for example).
There is an article where the writer explains this with good illustrations. You can find it by searching "the sweet science of candymaking" by "Tom Husband", published in the website "ACS".
I hope this explained roughly how the sugars work.
@@danielalt7508 Very nice to know the answer but is not usable within homemade mycology tools because almost everyone use glass a not pyrex(borosilicate glass) which is very expensive so cooling down quickly will result in breaking the regular glass container especialy in a pressure cooker when you cant release the pressure very fast for the risk of contamination and the sudden PSI drop can break the glass too
@@metarox69 Very nice insights. Also I just notice I wrote a little bit wrong on the previous message. Paragraph 3 is not meant to refer to a mixture of different sugars, but instead to a mixture of water and one sugar... I must have written that in confusion on the usage of the word "mixture".
Basically having 2 different sugars in one mixture, will never form crystals until a very long time has passed and the mixture is left untouched (for example honey that goes from liquid to solid). The rock candy is just one type of sugar (usually sucrose) mixed with water, and then cooled over long period of time.
This means that having a sugar combination of 2 different sugar types in an autoclave is not going to crystallize.
--------------
If I remember correctly, the mycelium absorbes easily monosaccharides (just like humans), which are fructose, glucose and galactose. The ideal sugar mixture would be a combination of at least 2 of these sugars for the mycelium, and mixing those will prevent crystallization.
@@danielalt7508 nice to know, will keep in mind when i have the best genetics from my APE ( probably years)
I got a 10cc syringe from a reputable dealer. I have used a grand total of 4cc from the syringe and in return I have 10 flourishing, clean agar plates and 3 quarts of popcorn inoculated. The remaining 6cc in the syringe is so dense with spores it's amazing. The agar plates are for isolating strong specimens, then the LC comes from my favorite from that. For a $21 syringe, you can do a WHOLE LOT. For sheer ghetto points, I have never even screwed the provided syringe on. I have used 4cc of spores completely bareback from the lurelock port. I observe reasonable aseptic techniques but do not have a laminar flow system. None of the 10 agar plates are contaminated.
Ghetto points awarded! 😂
@@omarlittle5802 10 clean agar plates noen of em dirty and thats ghetto? here come get ur stupid award lol 🤪
Where do you get the spores?
Lmfao 👍
Ghetto for the WIN!!!!!!!!!
Psychedelics are just an amazing discovery. It's quite fascinating how effective they are for depression and stress..saved my life.
Psychedelics definitely have potential to deal with health issues like anxiety and depression, I would like to try them but it's just hard to source out here.
He often interacts on insta, using the user below..
Ted_winston21
yeah mate... @ted_winston21
Remember to refer him after working with him.
Easiest, most straightforward video ive seen yet.🙏🏻
Yes the easiest way to get you a contaminated culture. Goof luck with the lack of knowledge. Never just watch ONE video and think you "got it down". You will waste time and money watching videos from amateur growers like this.
Give yourself another gift: If you tab the tape on the roll when you're done, it makes it easier to pull for next time, plus you already have your tab ready to go! Great videos and love your calm energy, thank you!
Thank you Jared for the All in one grow kit. Blessings to you and your family❤
Thank you! Blessings to you as well :)
You are a rare breed on RUclips, sir. Your videos are direct, not gimmicky, easy to follow, and delivered in a professional manner. Thank you for your efforts.
One question. What gauge is the blunt 4 inch needle?
Well! Thank you for the kind words!
The blunt tips are 12ga.
Brother, your videos are fantastic, informative and straight to the point! Thank you for all you do! ❤
As I am just starting out in this endeavor your video simplifies what I had always imagined could and is being done- thank you!
Your channel is the best on RUclips. We love you!
LME liquid malt extract will show growth in a couple of days, i used to use karo but LME is a game changer!
What a great pedagogue you are. Can't stop watching your videos. Little question here. Where (i guess fridge) and how long does your liquid culture keep?
Thank you, Nathalie! Correct, the fridge is where I keep the culture. I've kept them for up to 6 months in the fridge, though they may last longer. Six months is the arbitrary cutoff I've given myself to make sure they are viable when I inoculate.
So helpful for a noob like me. You’re the man!
What are the "nutrients" u inject in jar prior to pressure cooker process?
Corn syrup.
sperm
Watch pgt super liquid culture recipe on RUclips
This is the only video I've watched like this! Thank you!!
Well thank you! Glad you enjoyed it!
My question is, if in 6 months I take one of the syringes and repeat the process, will I ever need to buy any of that strain ever again? Should be repeatable forever, correct?
Anyone know if this is correct
@@scana7811correct
@@scana7811i heard somewhere that after a couple times you renew it the mycelium will degrade but don’t bank on that info I might be wrong!
It takes 1 to 3 weeks for lc to colonize. Thanks for the updated steps!
No it doesn't, it can take as little as 3 days
@@FantomFirez Multispore Syringe to Liquid Culture will take 10-21 days.
Agar to Liquid Culture or Liquid Culture to Liquid Culture will take as soon as 3-5 days.
Multispore Syringes have to germinate first. Then after germination, they have to find other nearby spores through sending hyphal threads outwards in search. Multispore Syringes as a result take 2 - 3 times as long to colonize than any other method.
And your Multispore Syringe/the spore print that was used to create said Multispore Syringe could have had contamination that you will not have noticed until very far later and very much further down the pipeline of growing, nearing fruiting conditions or right after spawning to bulk substrate.
Nice work! Getting ready to do some of these myself and the information is very appreciated.
If I only have 1cc of liquid culture in a syringe will that be enough to start a new jar of liquid culture?
Very interesting. I'm just starting to learn all this stuff and that was very helpful. Thank you.
I am just learning also after using kits for a while
Whilst viewing a different video, it was recommended to use a magnetic shaker and magnetic 'pill' every day and not to leave it for a week before checking on anything. Any thoughts?
Just wanted to add on the contamination worries especially for us first rate newbies,.. if its too overwhelming to make mason jar blenders and opening jars etc,.. make the liquid substrate jar with an injection port,.. and inject bought liquid syringes.
This is not to knock the poster’s skills,.. i just find this video very advanced compared to where i am at this point in time. Also i live in a 200 year old house from hell (building a new place thankfully) but theres every possible way to contaminate in this house i am in right now. It would increase my chances of success if i bought a pre done spring and injected it into a sterile jar of liquid culture. I can learn to make that jar, to add nutrient and inject sterile air etc. but making the Petri dish, cutting it etc thats a ton of opportunity to add contaminants when done by me😊.
You seem pretty chill. Going to check out more of your videos.
Did you use a cabinet against air germs?
Can you transfer a grow bag with mycelium to a monobox with substrate? Ty
Thanks for the videos im on 15days n there is still alot of spores in my jars did i do something wrong infollowed everything to the t
can i use honey? in place of corn syrup thanks and keep up the good work
Yes, definitely can use honey. I haven't seen much difference other than the honey cultures seem to have more sediment, which makes it more difficult for me to tell if there is contamination. But, it's always worked fine for me when I've used honey!
Thank you for the kind words!
You can also use your grain water. Ive done that with LC
@4:35 - that is the most beautiful Snow Globe ever :)
do instant pots work in place of a standard pressure cooker?
No. You need to bring it to 15 psi to reach the proper temperature to sterilize the liquid
Great video. Thank you for your information. So now you have 10 30cc syringe where you drew mycelium (not spores in through a 12ga needle. When you are ready to dispense into spawn jars or bag,, do you have to use same sized 12ga needle? Or a more standard sized injection needle?
I take off the 12ga blunt needle and use a 16ga pointed needle when I’m ready to use. Has worked for me pretty well.
Thanks. I had given up on trying to cultivate at home until watching this.
Thank u so much this looks so much simpler than I had anticipated
Really great hair day for sure bro. That's really all that matters in life
May not hear from you years later but I've a question. So the 70% alcohol spray won't hurt the spores on the syringe/liquid culture?? Nice video!
If I want to use peptone and malt extract how much of each do i use?
Thanks for this awesome video, I gotta question, can I use Honey instead of kernal syrup ? If not, what else can I use in case I dont find kernel syrup in my country please
Can you start this expansion with a syringe of liquid culture rather than a spore syringe?
I was wondering the same I hope we get an answer
I am planning to test this theory soon I will tell you my results
What kinda nutrients do you use in that first syringe?
You made it look so easy!!
Does the alcohol not harm the Micelium or the Spores?
In order to get mycelium growing the quickest… is it faster to turn spore syringes into LC rather than just inoculating my grain with the spore syringe?
Awesome video dude! Question, I want to do this but syringe is a live culture syringe with mycelium in it instead of spores. Can I do the same process and just inject the jar with the mycelium I already have instead of spores?
Can you do this without a pressure cooker? Meaning using a different way to accomplish the same thing, not just skipping what it does.
The first thing that comes to mind is using an instant pot, but I don't think that's the essence of your question...
I'll have to do some thinking on this, I'm sure there's another way, but everything coming to mind involves heat sterilization.
@@OneEarthMushrooms I guess the essence of my question is I don't own a pressure cooker and would like to avoid buying one. I should have added that.
Take a pot and put jar rings on the bottom fill with ur jars and then add water to the bottom of the jars. Cover and bring to a boil then put it on a simmer for 60-90mins then let cool
Look up broke Boi tek and he has videos on no pressure cooker sanitized jars. I do it. Works great. Just let it stay in long enough. Also make sure ur jar lids are lose so the glass doesn't crack
ruclips.net/video/UgCW-ZPkxIU/видео.html
The alcohol not being dry before injection doesn’t kill the spores? How?
I’m confused. Did you not chance contamination with the spoon and syringe by pulling liquid into and out of that same syringe when you added nutrients ? Or was that off camera ?
The pressure cooker killed all contaminants before spores introduced to the liquid in the jar.
So if I already have a 10cc syringe of LC, how would I multiply that. Would I follow the same steps, just instead of 10cc of MS use 10cc of LC?
What I would do is use most of the syringe to innoculate grain jars and use the last few CC's to make a LC jar and refill your empty syringe.
Nice hair. So do you see contamination in the LC or do you just roll the dice? Is it safe to assume that the same thing can be done from LC to LC? How manc CC of LC would you add to 800ml of solution?
Hi there, I have a question: Is there any other sweet nutrient to replace the corn syrup or does the nutrient have to be exactly corn syrup? Thanks for your advice!
Honey
THIS IS ONE THE BEST CHANNEL I HAVE COME IN CONTACT , PLEASE TELL US THE NUTRIENT YOUR USING . IS IT LME PDA SYRUP HONEY PEPTONE??? REPLY BEFORE I MAKE MISTAKE THANKS AND REAMEN BLESSED
So? Mycelium can be broken up and reform, kinda like a sponge? (Sea Sponge can be divided into indiv. cells, and reform) I thought if I broke it up too much I'd be killing some, or at least slowing it down.
Great tutorial as always. I am wondering how many, lets say 1 liter grain jars (filled 3/4th) can you inoculate with a 30 cc syringe? or is it a syringe per jar.
For a 1 pint jar of grain, use 1-3cc or 1-3ml of the syringe. So technically a 30cc/30ml syringe can inoculate 30 different jars if you wanted to do that. Also store the LC in fridge when you're done and it will last 6 months to up to like 2 years if my memory serves me well
@@DamnBrother Thankya brother
@@DamnBrother how much fruiting substrate do you use per pint jar/pound of grain spawn? New to this trying to figure out how much of each thing to get 😂
@@oliviafinnegan2437 everyone has different recipes but mine I use 200 grams vermiculite, 200g coco coir and 40g of gypsum. I weigh this all out dry and pasteurize with about 8 cups of water. This is enough substrate to use in a 33qt tub, and I use 1-2 pint jars of grain spawn. 2 is sort of overkill but hey it's just me.
@@DamnBrother I needed a good starting point and I’ll adjust as needed ◡̈ thank you so much for this
I don't have a pressure cooker. Can I use a rice cooker?
Can you use jelly powder(no gelatin) instead of agar powder in Petridish? Pls reply fast
why is your alcohol green ? is there other chemicals added ?
Thank you, using your website. Enjoyed the video, like your style.
I really hope you see this. Do you have to use a pressure cooker or are there any low and slow methods that would do the same?
instant pot
Hi Kayla! An instant pot will work well too. I have on my list of experiments: "microwave sterilization of liquid culture" but I haven't gotten there yet.
Thanks for sharing this concise and informative coni👍🏼
Can I make 2 of these small jars with one spore syringe or do I need to do all spores into one jar
do i immedietly inject or do I have to store it for like a week, or two?
I'm assuming I should be using distilled water for the solution? Or is tap water just fine?
Distilled. The chlorine in tap can kill mycelium
Thank you for reporting in grams and millimeters. :)
Hi, what kind of nutrients do you use and where to get them?
I'll recommend you to this specialist who has been my guide on them he got all kind of Mushrooms stuffs and ships to any location also guides newbies.
*@VINSPLACE,* that's the handle*
Dude Is Available On Telegram?
You crack me the hell up, love you broski.❤
Just wondering if the pressure cooker is absolutely necessary here? Can I just use my normal Tefal pot?
GOOD LUCK TO YOUR WORK SIR, AM ABOUT TO START NOW , THE NUTRIENT YOUR USING IS IT LMA OR AGGA AGA. NUTRIENT AGGA ? PLEASE REPLY BEFORE I MIX . THANKS AND REAMEN BLESSED
Would honey be a viable source of "nutrients"..?
Great video I spend like 50.00 at a time on syringes....do you use distilled or bottled water for fruiting and mycelium instead of tap
Naa I just use tap water. I've had great success, and don't see any need to pay a giant corporation for processed water.
Could you do this over and over with the same parent syringe, so you have liquid culture forever?
Can i use lc instead of spores? Just putting 10cc Liquid culture in the jar?
Can I use another sugar instead of the corn syrup?
Going to be doing this today in an instant pot. Do you put the jar right on the bottom of the cooker or do you put the insert in so the jar is not directly in the water ?
how did it go did it work and what setting did use for your instant pot?
Wait why are you using the vent hole instead of the injection hole to collect your liquid culture?
Why do you need a pressure cooker, can you do it without one?
Can u just keep the LC in the jar instead of all them 30cc syringes ty 😊
Spraying the syringe cap wont kill your lC?
Cool video bro, live culture rocks!
Would you knock a jar up with the whole 10cc of spores, or how much per jar? Plan to use 600ml LC.
I've got a question, Would this work by using a 💉 of liquid culture? Or does it have to be done with spores only?
Also, I wish you sold liquid culture on your site, I'm in the market for some as I am about to do some research pretty soon.
❤
What would be the preferred gauge of the dispensing needle? About to dive in!
Scrolled down and found the answer, Thanks for the vid!
I had an issue or problem that maybe someone has suggestions for. I had two setups, identical to yours in a pressure cooker, and water boiling at 20 psi (vs 15) caused rock to jump around tapping the bottom of the jar and splitting the bottom off both 312mg setups. Just like tapping glass for a controlled break the bottom was 360 tapped and broke. Any suggestions other than not have a rock in there? Great sterile technique. My water level was a little deeper than yours and just touched the glass bottom
What nutrients are you using please?
Honey and or corn syrup
@@randymetz2616 Thanks Randy. Relative nnob here. x
Me too 😂
5:00 is the alcohol in the syringe not going to damage the cultures?
Great video, mate. So simply communicated.
I live In the UK and Corn syrups are called high fructose corn syrups and isn't easy to find due to production quotas. Are there alternatives?
Use organic honey
@Journey Through The Mycelium Network I didn't ask about the malt extract lol I already knew about it. Thank you tho
does it matter if using tap water or distilled? thankyou!!
It shouldn't since you're pasteurizing the water in the pressure cooker, but with all the potential crap in city tap water (lead, microplastics, etc.), wouldn't distilled be the better choice?
What happens when two types of spores are mixed in the culture?
How many cc’s of the liquid culture would you add to a grain bag then?
What exactly are the nutriants you are adding to the water?
Is that a meteorite chunk? 2:10 ? Can I just pour some leftover dust in a jar and sterilize? I have a magnetic spinny hot plate. Thank you
The first syringe you used is the “12 grams of light corn syrup” right?
Do the alcohol that's left over doesn't kill off the mycelium?
@Ramsey Nancy Don't care about psychedelic. I need to kill cancer.
Can I use liquid culture instead of spore syringe?
Can someone explain why he injected the needle to pull out mycelium through the air holes and not the injection port? Any insight is appreciated
He used a long blunt needle to get all of it out. It wouldn't go thru the injection port.
Does anyone know a substitute for corn syrup? For some reason I can't find any in my area.
Also how long to keep the jar before extraction of the mycelium after all the process shown in the video?
Do you admin 10cc liq cult into 5 lb all-in-one. The same as you would 10cc spore?
Hey man! Is the rock special or is it just for mixing the LC around instead of using a magnetic mixer?
Do you need a pressure cooker?
Can I also do this method using only 2.5ccs?
How long can it stay in the jar before putting in syringes
Can this be done without a pressure cooker?