600 ml H2O 18 g corn syrup 1.2 g light malt extract 1 g peptone Stir to dissolve. Add stir bar (or stone) Cap jar and wrap lid in foil. Pressure cook @ 15 psi for 30 minutes. When completely cooled, inoculate. (Took notes for myself, but also wanted to say thank you for the video!)
@@cyberbid not my video, but I use it to culture a LSS into mycelium, and then I put a sample on an agar plate to test for contam. If it grows clean, I go back to the liquid culture and set the mycelium to grain spawn. You could use a piece of a fresh cap, but might have a higher risk of contamination. Good luck!!
This Super LC recipe along with the BB brown rice tek has yielded fastest jar colonization times for me. I have dime size growth in 8-10 hours, 50% (shake) at 4 days and ready for bulk at 10-12 days. I use PGT 🧬 and go straight to super LC. Thank you for the time you put into these sir.🍄👍
@@PhillyGoldenTeacherquick question about the 15 psi. I live at lower elevation and my pressure cooker for all recipes is 5 psi due to elevation. Should that also apply to using in making LC?
Instead of mixing all the ingredients with a spoon at the end, drop the mixing bar in first and leave it on the mixing plate, spinning, while you add ingredients to make it a little easier on yourself.
Thanks a lot Sensei!! I used your technique from this video and have got amazing results. I have super healthy liquid culture!! Keep up the great work 👍
I had oyster mushroom culture on a petri dish for longer than 2 years in the fridge abd it still worjed perfectly. So in liquid culture it should last forever basically.
This lc recipe was the first time I ever got a lc to work. Not saying that others don’t work. But the way you explain and the order you do it, really just everything is awesome 🤙🏽🤙🏽🤙🏽🤙🏽🤙🏽
I love how you say you are using quart jars, but then go onto measure 600ml and the other measuring cup is in milliliters too. Metric reigns supreme! Keep up the hard work!
Or a marble in there or something.. Worst case you can draw some.of the LC up and squirt it out and draw it up, it'll help break up the myc...I tried a few times. 1 didn't stir or shake enough it ended up being a huge golf ball. The other jar I did with that 1.i shook enough. It looked like a sow globe!!
I quickly grabbed a marker and wrote your recipe down.Thank you.Twas short and precise.I'll follow it to the letter.I dont have a pressure cooker yet so i tried with rain water and some honey and I used my favorite sugar substitute,organic Agave.Any comments on that?I believe it should work.Thanks again for your video.I liked and subscribed.PEACE.
I seem to have done almost the same research you did and went about things very similarly. However I used dry corn sugar, the four pound bag, and made a pre-mix with the dry malt extract. I also cooked for 40 minutes because I figured a darker solution would be good for the mycelium, but it certainly is harder to see what is going on.
Thanks a ton for your videos. You helped me tremendously when I was starting out and I still check in to compare notes. What’s the deal with peptone? I’ve been incorporating hydrogen peroxide a bit to reduce contam, but what’s your experience with peptone?
I'm looking into modified plastic lids for my first time making LC, but kind of confused about those red syringe filters. Are they used to suck out the LC into the syringes?
Brother, thank you so much for making this. I am hyped about trying to grow my own Oyster mushrooms and more. Mushrooms are so dope and interesting. Props, peace, and blessings to you and yours in the name of The Lord Jesus Christ. amen
I see that we put the foil over the lid to protect the filter, from water right? Does the water in the jar not create steam from the inside while pressure cooking, and wouldn't that get the filter wet either way?
I've recently seen a few videos where they REDUCE the nutrition in the agar mix, and claim it increases chance of rhyzomatic mycellium. The idea is reduced nutrition causes the mycellium to have to stretch out to find nutrients. Too nutritional of an agar mix might cause nothing but the fluffy pomentous (sp?) Mycellium to grow in your plate...
I got hiveg peptone which is recommended over the stuff used here but every attempt I’ve made has turned sour, cloudy and stunk bad. Leave the peptone out and it’s fine.
Hello Bro. Hope you're doing well. What are your thoughts on just adding peptone to like your regular corn syrup LC. For example -i got premix LC stuff. Agar powder. Peptone. Shit ton of honey ect. Is peptone just a good gram to add to it all? Take care man🍄🙏🤘
I only use corn syrup when I need to isolate from contams caz it hampers myc growth a LOT. only benefit is that it is clear. my goto is just LME + wyeast . making real potato tea is really really good. Sometimes I will use oak twigs(remove bark/make tea) as a nute boost if the stain needs to be re-invigorated.
Hey what RATIOS do you use for just LME and (nutritional) yeast? One guys saying 10 grams per500ml I think!?! I like to fill to 600ml.. can u help!? Thanks!❤🍄
they all have different outcomes for what u need to do. if ur just starting, u really can use anything that has sugar or starch. ur main concern is cooking to sterile. getting ur stuff clean & sterile is the main concern. after that just experiment and take note of what happens. yeah, LME is a luxury/not needed.@@SachaLotus
potato is hard to get clear if I remember correctly, so if u need to see whats going on i would suggest something more pure like karo. i do remember potato being fast tho. the myc said yum yum, i like potato. the karo, is a little slower(but crystal clear) and can give more time to work like do an isolation or separation. i use a clear LC to find the winners and make sure everything is clean. once u get a colony that is hardy hungry and ready to rock, use a nute mix that has fast growth for expansion. the LC mixture that is used for expansion might not be clear or have a good color, but it'll get consumed fast so u can achieve a good volume. ofcourse, its vital to get it clean first so u gotta b able to SEE it in the beginning. i suppose u could do all the work on agar, but doing that work in the LC is my goto technique. plus agar is a lot of steps.. i do all that in the LC and have gr8 success. I like the small surface area of jars instead of the wide agar plates consider > those little guys are hungry and stuck in their home. they will eat whatever caz they gotta survive. I bet even white sugar will work. p.s remember to leave one jar NOT innoc'd as a control so u know ur technique was good. do all same steps, just no innoc. -love and light@@SachaLotus
@@tdtrecordsmusic thank you so much for taking the time to reply 🙏, I really appreciate it. I ended up doing two different recipes. For one I used a 2% honey, and on the other one I used 2% honey plus a tiny bit of potato tea plus less than 0.1 gram of a collagen capsule (I read in a comment that it’s basically peptones) . So wish me luck and hopefully they all do well. I’m still gonna do some agar plates to test for contaminations in the near future. Great tip about leaving one LC jar not inoculated to test for contams. Mush love from Peru 🇵🇪🙌🏼🙏
Hey may jar came out...and its like GOLD! Like super dehydrated urine color...Is that ok or did I add to much Malt or syrup any help would be appreciated.
Has anyone tried preparing grains with peptone? I’d be wary to add it to substrate for contamination risk, but seems like it could help grains colonize faster and potentially promote more vigorous growth?
I use 5g per 5lb dry in my simmer, also found that brown rice is a banger for nutes, so when I make 1lb bags (basically a full quart) I throw in 1 tbs of dry brown rice before I pc, I will never go back
I’m doing it on my instapot and every time the glass is breaking at the bottom I think the glass was too cold even tho I used warm water to do it . I also let it cool overnight and still . This time I warmed up the jar on a pot for like an hour at 110* and used the same hot water to do my LC I’m waiting for the pc to finish now and see what happens. Any suggestions? ( I’m using the tray to the pc too)
Would this recipe work if inoculated with tissue instead of agar? I keep getting very murky LC after about a day or two and was wondering if the peptone made it more prone to contam with tissue vs agar sample.
Hi, I have used this culture recipe and its excellent so thanks for that, your a really good teacher. A quick question? I've just PC'd some culture but only used Karo syrup in the correct quantity as your earlier video, just to try. Did the normal stuff and PC'd for 25 mins at 15 psi, but the culture came out an amber color, but very clear. Any ideas why this is??? Much appreciated. Tom
Thanks for the new and improved recipe. I hope it works out, I bought some peptone yesterday, the same in this video. It STINKS 🤥 is it suppose to stink?
Greetings Golden Sensei, I was wondering if it would work if i Swap the sugar provider from corn syrup to powdered dextrose? Thank you for everything you've taught us 🍄💛
What kind of mix is that? Here in Germany it is relatively difficult to get peptone. It is simply reserved for laboratories. But I got some and did this LQ. Within 12 hours a solid mycelium carpet has formed. How fast is that !?!?
Hey my friend- question- if I buy a Pre-made liquid culture, inoculate it do I NEED- an electric spinning centrifuge to 'spin the culture' with a magnetic piece inside the jar or can I just shake it by hand periodically??????????????
How do you visually identify contams? Mold looks so similar to mushroom mycelium, and I have some growth that looks like mycelium but is... threaded differently idk how to explain it all clumps with the normal looking mycelium too
600 ml H2O
18 g corn syrup
1.2 g light malt extract
1 g peptone
Stir to dissolve.
Add stir bar (or stone)
Cap jar and wrap lid in foil.
Pressure cook @ 15 psi for 30 minutes.
When completely cooled, inoculate.
(Took notes for myself, but also wanted to say thank you for the video!)
Thank you for writing that out.
How do you inoculate this mixture with mycelium? Can you use part of a fresh mushroom?
@@cyberbid not my video, but I use it to culture a LSS into mycelium, and then I put a sample on an agar plate to test for contam. If it grows clean, I go back to the liquid culture and set the mycelium to grain spawn.
You could use a piece of a fresh cap, but might have a higher risk of contamination. Good luck!!
🧑🌾
I wish they’d use cups, teaspoons, 1/4 teaspoon etc. instead of weight measurements. 12 ml, 18 grams…, How much is that?
This Super LC recipe along with the BB brown rice tek has yielded fastest jar colonization times for me. I have dime size growth in 8-10 hours, 50% (shake) at 4 days and ready for bulk at 10-12 days. I use PGT 🧬 and go straight to super LC. Thank you for the time you put into these sir.🍄👍
I'm glad the recipe helps! Seems you are doing really well with your journey. Keep up the great work! 😁
@@PhillyGoldenTeacherquick question about the 15 psi. I live at lower elevation and my pressure cooker for all recipes is 5 psi due to elevation. Should that also apply to using in making LC?
@@BeeCJ I'll chime in, just go for 15psi you should be good
It really is top notch ..... I haven't had anything take over 2 weeks to fully colonize, it just spreads like wildfire it's gorgeous
@@PhillyGoldenTeacherIs there any recipes for making LC without a pressure cooker? I have one that only reaches 5psi
You're the man philly! Keep feeding us the knowledge. Much appreciated.
Thanks for the tutorials! I’ve been using this recipe, only using honey instead of corn syrup. Excellent growth!
thanks for the feedback. I bought peptone yesterday and it stinks. Is it suppose to smell?
@@michaelkreisel3573 good habit in a lab is DONT smell stuff.
How much quantity of honey you have been using?
@@michaelkreisel3573 yes
today i found a jar in my lab fridge with over 1 year old mycelium-perfectly fine and strong..great stuff
This is the only lq tech i will ever use healthy mycelium never had contamination tried and tested 100% works
Thank you for all your work! About to start fruiting conditions on my first tub and it’s been smooth sailing so far!
How were they??
Ya how'd it go?
How smooth was it?
I use organic brown sugar and it works good too. Great video!
600ml water
12ml Syrup (18-20g)
LME 1.2g
1g Peptone
magnetic stirbar
30min pressure cooker
*At 15 psi
Is this recipe per 1qt jar ? How may jars can this make ?
Lol nevermind
600ml @@MycoTooMushed
12 ml is 12gr not 18-20gr
Instead of mixing all the ingredients with a spoon at the end, drop the mixing bar in first and leave it on the mixing plate, spinning, while you add ingredients to make it a little easier on yourself.
The corn syrup always messes up my stir bar if I do it this way
Pgt taught me how! I’ve had much success thanks to this man. Cheers bro.
Just got a pc. So stoked that I can make everything myself now! Thank you for this video. It's so simple!
Way to go, pgt! Really enjoy your videos, you're helping me learn the right way as fast as possible.
Thanks a lot Sensei!! I used your technique from this video and have got amazing results. I have super healthy liquid culture!! Keep up the great work 👍
Hey PGT, what are your thoughts on liquid innoculant vs liquid culture?
I had oyster mushroom culture on a petri dish for longer than 2 years in the fridge abd it still worjed perfectly. So in liquid culture it should last forever basically.
This lc recipe was the first time I ever got a lc to work. Not saying that others don’t work. But the way you explain and the order you do it, really just everything is awesome 🤙🏽🤙🏽🤙🏽🤙🏽🤙🏽
That's awesome, I'm glad you got it to work! 😄
Having problems getting peptone, is there another name for this product?,keep up the great work much appreciated..!!thank you.
4:15 chuckling just a little bit at stirring the mixture, before putting a stir bar in 😅
U the goat!!!! I followed this tutorial and meeeeen the grow is noticeable STRONG!!!!! THANK YOU !!!
😎🍄
Love Mossy and the amazing PGT!
🙏🍄
Ive seen this channel since it had two videos, I’m attracted to your voice. Keep coming with the vids.
He does have a very professional voice, handsome, dare I say
Pause
Gay lol
@@llaves973 very 🤤
Gayyy
sensei your words inspire !
Thank you for your time,should incubation process be in the dark , or doesn’t really matter?
I love how you say you are using quart jars, but then go onto measure 600ml and the other measuring cup is in milliliters too. Metric reigns supreme! Keep up the hard work!
Just an idea 💡 instead of stirring it with the spoon, why not use the magnetic stirrer? 😁 good content! 👍🏽💯🍄
Or a marble in there or something.. Worst case you can draw some.of the LC up and squirt it out and draw it up, it'll help break up the myc...I tried a few times. 1 didn't stir or shake enough it ended up being a huge golf ball. The other jar I did with that 1.i shook enough. It looked like a sow globe!!
That’s what I just did instead of spoon. I’m sure it will work the same
I quickly grabbed a marker and wrote your recipe down.Thank you.Twas short and precise.I'll follow it to the letter.I dont have a pressure cooker yet so i tried with rain water and some honey and I used my favorite sugar substitute,organic Agave.Any comments on that?I believe it should work.Thanks again for your video.I liked and subscribed.PEACE.
I seem to have done almost the same research you did and went about things very similarly. However I used dry corn sugar, the four pound bag, and made a pre-mix with the dry malt extract. I also cooked for 40 minutes because I figured a darker solution would be good for the mycelium, but it certainly is harder to see what is going on.
And it failed im sure. Utter clown.
Great to see you back! Happy harvesting!
The reason why this works because many fungi optimal carbon source is malt extract and optimal nitrogen source is peptone / yeast extract
You’re an amazing teacher and I love your video style & the effort you put in
Thanks for the info. Very concise and useful!
Left a like too brother, thanks for helping me on my journey to my first success harvest, now looking to start it all over again, thanks so much
Love the peptone, thanx❤
Great video! Thanks for everything you do. making some LC today :)
Thanks a ton for your videos. You helped me tremendously when I was starting out and I still check in to compare notes. What’s the deal with peptone? I’ve been incorporating hydrogen peroxide a bit to reduce contam, but what’s your experience with peptone?
Glad to see a new video bro.
Keep up the good work!
Just in time! Modified some lids for LC today. Thanks for all your knowledge Golden🤙🏻🤙🏻mush mush
I'm looking into modified plastic lids for my first time making LC, but kind of confused about those red syringe filters. Are they used to suck out the LC into the syringes?
@@chicofromph33nix64 You use the self healing injection port. Syringe filter is for fae
I use light malt extract and insect frass. I might add fish hydrolysate (peptones) when making my next LC.
Can’t wait to whip some up !
Brother, thank you so much for making this. I am hyped about trying to grow my own Oyster mushrooms and more. Mushrooms are so dope and interesting.
Props, peace, and blessings to you and yours in the name of The Lord Jesus Christ. amen
killing it this week
put 10cc of the thick solution as a jump-start and then put constant stirring on for 24-48 hours, it will look almost entirely colonised
Peptones are key.
Looking forward to trying this tasty recipe. Grazie mille sensei.
Love the instructions man great video. Thank you! I've been trying this and keep getting a Grey mold floating on the surface. Any tips?
I see that we put the foil over the lid to protect the filter, from water right? Does the water in the jar not create steam from the inside while pressure cooking, and wouldn't that get the filter wet either way?
Thanx Philly GT!
Been using peptone for about a year all because of Mossy Creek!!!
Hey, thanks bro. Appreciated.
Nice vid philly G!
I haven’t changed up my LC recipe for over a year but today I am trying this one.
Is this your preferred recipe?
PGT thanks for all that you do 👍 🙏
I wanted to know if adding peptone bacteriological to your Agar recipe would have a positive effect on my plates?
Of course it will 🔐
I've recently seen a few videos where they REDUCE the nutrition in the agar mix, and claim it increases chance of rhyzomatic mycellium. The idea is reduced nutrition causes the mycellium to have to stretch out to find nutrients. Too nutritional of an agar mix might cause nothing but the fluffy pomentous (sp?) Mycellium to grow in your plate...
I got hiveg peptone which is recommended over the stuff used here but every attempt I’ve made has turned sour, cloudy and stunk bad. Leave the peptone out and it’s fine.
Simpel as always, big thanx!.
Man these videos have taught me everything . Is this LC good for all mushrooms ?
Love it !! 🍄🍄🍄🍄
Very good video, thank you.
Aloha! Thank you for always educating! I learned tons from your video! 🤙🏿
happy new years broski
Excited to try your method, I have had 2 unsuccessful attempts at LC. Agar and prints have been so easy for me but this has evaded me. THANK YOU
do you share your prints?
Yes he does 😂 lol …. How’s the library comin along guys!? Spore trading / selling itself is not prohibb’d!! Trade away!
Hello Bro. Hope you're doing well. What are your thoughts on just adding peptone to like your regular corn syrup LC. For example -i got premix LC stuff. Agar powder. Peptone. Shit ton of honey ect. Is peptone just a good gram to add to it all? Take care man🍄🙏🤘
Thx for this... i had growth from spore in 3days
Thank you again PGT :)
My LC is coming out the color of Miller Highlife after PC'ing. Idk how yours comes out so nice and clear.
I only use corn syrup when I need to isolate from contams caz it hampers myc growth a LOT. only benefit is that it is clear. my goto is just LME + wyeast . making real potato tea is really really good. Sometimes I will use oak twigs(remove bark/make tea) as a nute boost if the stain needs to be re-invigorated.
Hey what RATIOS do you use for just LME and (nutritional) yeast? One guys saying 10 grams per500ml I think!?! I like to fill to 600ml.. can u help!? Thanks!❤🍄
So u use potato tea instead of LME? I live in South America and can’t really get Lme or peptones.
they all have different outcomes for what u need to do. if ur just starting, u really can use anything that has sugar or starch. ur main concern is cooking to sterile. getting ur stuff clean & sterile is the main concern. after that just experiment and take note of what happens. yeah, LME is a luxury/not needed.@@SachaLotus
potato is hard to get clear if I remember correctly, so if u need to see whats going on i would suggest something more pure like karo. i do remember potato being fast tho. the myc said yum yum, i like potato. the karo, is a little slower(but crystal clear) and can give more time to work like do an isolation or separation.
i use a clear LC to find the winners and make sure everything is clean. once u get a colony that is hardy hungry and ready to rock, use a nute mix that has fast growth for expansion. the LC mixture that is used for expansion might not be clear or have a good color, but it'll get consumed fast so u can achieve a good volume. ofcourse, its vital to get it clean first so u gotta b able to SEE it in the beginning. i suppose u could do all the work on agar, but doing that work in the LC is my goto technique. plus agar is a lot of steps.. i do all that in the LC and have gr8 success. I like the small surface area of jars instead of the wide agar plates
consider > those little guys are hungry and stuck in their home. they will eat whatever caz they gotta survive. I bet even white sugar will work.
p.s remember to leave one jar NOT innoc'd as a control so u know ur technique was good. do all same steps, just no innoc.
-love and light@@SachaLotus
@@tdtrecordsmusic thank you so much for taking the time to reply 🙏, I really appreciate it. I ended up doing two different recipes. For one I used a 2% honey, and on the other one I used 2% honey plus a tiny bit of potato tea plus less than 0.1 gram of a collagen capsule (I read in a comment that it’s basically peptones) . So wish me luck and hopefully they all do well. I’m still gonna do some agar plates to test for contaminations in the near future. Great tip about leaving one LC jar not inoculated to test for contams. Mush love from Peru 🇵🇪🙌🏼🙏
Is this diff from Mossy's formula and if so did you find yours better?
Mr.PGT, can I use water from my well since there’s no chlorine in it? It has minerals in it though. I’m loving your videos! ❤
Yes you can 🙏
your videos are really helpful.thank you🤘
Ive been bingeing your content this week. What a happy surprise this morning
Philly, I picked up the olive stuffer from your Amazon links for agar punching - how do you sterilize it?
Vielen Dank.
Hey may jar came out...and its like GOLD! Like super dehydrated urine color...Is that ok or did I add to much Malt or syrup any help would be appreciated.
Has anyone tried preparing grains with peptone? I’d be wary to add it to substrate for contamination risk, but seems like it could help grains colonize faster and potentially promote more vigorous growth?
I use 5g per 5lb dry in my simmer, also found that brown rice is a banger for nutes, so when I make 1lb bags (basically a full quart) I throw in 1 tbs of dry brown rice before I pc, I will never go back
I’m doing it on my instapot and every time the glass is breaking at the bottom I think the glass was too cold even tho I used warm water to do it . I also let it cool overnight and still . This time I warmed up the jar on a pot for like an hour at 110* and used the same hot water to do my LC I’m waiting for the pc to finish now and see what happens. Any suggestions? ( I’m using the tray to the pc too)
good info 😮
should i leave it alone till i see growth or should i stirred every day even before anything grows ?
what are the downside of doing "sterilized in a steambath with a pot and a lid" cause i dont have much to spend for a pressure cooker?
Would this recipe work if inoculated with tissue instead of agar? I keep getting very murky LC after about a day or two and was wondering if the peptone made it more prone to contam with tissue vs agar sample.
Great video! Any tips on what to look for in terms of contams?
In the UK corn syrup isn't really readily available or cheap - is there a substitute that can be used? Honey maybe?
Hi, I have used this culture recipe and its excellent so thanks for that, your a really good teacher. A quick question? I've just PC'd some culture but only used Karo syrup in the correct quantity as your earlier video, just to try. Did the normal stuff and PC'd for 25 mins at 15 psi, but the culture came out an amber color, but very clear. Any ideas why this is??? Much appreciated.
Tom
Sugars caramelize with high heat.
Thanks teach!!!!🙏🏻
Thanks for the new and improved recipe. I hope it works out, I bought some peptone yesterday, the same in this video. It STINKS 🤥 is it suppose to stink?
yes lol
It stinks
Nice informative vid!
Nice thanks for the information
Greetings Golden Sensei, I was wondering if it would work if i Swap the sugar provider from corn syrup to powdered dextrose? Thank you for everything you've taught us 🍄💛
You can use honey, molasses and Karo syrup. I also had a little bit of instant potato mix for nutrients.
How much potatoes?! That's crazy. And it works?
I was literally looking for comments if anybody has use potato water in addition to the honey/corn syrup
Thank you
PGT, is it possible to substitute the corn syrup for organic honey? Thanks dude! Love your video! 10/10
Yes.
What kind of mix is that?
Here in Germany it is relatively difficult to get peptone. It is simply reserved for laboratories.
But I got some and did this LQ. Within 12 hours a solid mycelium carpet has formed. How fast is that !?!?
If I use this recipe and add agar powder to set it. Will this work as petri agar and slants?
Can you use glucose syrup instead? It’s difficult to find corn syrup in Denmark.
Same thing, I believe. From everything I know, you want a glucose source. Hence, why the dextrose in potato dextrose agar.
Loved it ❤️☺️☺️
Hey my friend- question- if I buy a Pre-made liquid culture, inoculate it do I NEED- an electric spinning centrifuge to 'spin the culture' with a magnetic piece inside the jar or can I just shake it by hand periodically??????????????
Should extra malt be added if theres no peptone?
So a
Alot of sandy particulates is ok to be sitting on bottom after jars sit for little while??
Teacher, can i use soy peptone ? thank you very much :)
How do you visually identify contams? Mold looks so similar to mushroom mycelium, and I have some growth that looks like mycelium but is... threaded differently idk how to explain it all clumps with the normal looking mycelium too
I use the same recipe but I like to add a pinch of calcium as well