My New Favorite Liquid Culture Recipe for Growing Mushrooms
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- Опубликовано: 6 авг 2024
- In this video, I'm excited to share with you my new favorite liquid culture recipe for growing mushrooms. It's a simple yet effective technique that results in a clear solution and promotes faster and more consistent mycelium growth. By combining just 1/64th teaspoon of peptone, 1/64th teaspoon of light malt extract, and 1/2 tablespoon of corn syrup or honey with 600 ml of hot distilled water, you'll be amazed at the results. Join me as we explore this recipe together and unlock the potential for incredible mushroom cultivation. Let's dive in and discover the secrets behind this game-changing liquid culture recipe!
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I used this exact recipe. Used 5ML of a LC into 600ml and its been 6 days i can not beleive how muxh the myc has grown! It is blowing my mind
Thank you brother! Peace and love☮️❤️🙏🏻
Your results are fantastic looking, and I LOVE the simplicity of your recipe. Most I have read and tried are far overly complicated in comparison. I can't wait to try this out! Cheers!!
Feel free to add more ingredients, you can use 1 gram of LME and .5 grams peptone for stronger solution. Just make sure there is enough water in the pressure cooker so that the water level is touching the bottom 2 inches of the jars while pressure cooking. I used low amount of ingredients because I kept getting sediment but found that I wasn’t using enough water in the pressure cooker which caused the sediment.
Funny story...been watching your videos and ordered all the stuff in this one so that I can start growing my own liquid culture. I just went on etsy and randomly selected a liquid culture syringe to get me started. I didn't even look at who the seller was until I had sent the payment. Turns out it was you XD
That is funny! 😂 Thank you and good luck with your experiments! 🧫 🍄
I stuck spores from a MSS into coconut water as an experiment and it started growing myc after 4 days.
I love how this comment section is basically a huge list of FAQs *that are being answered!*
Keep it up, man. You've earned another subscriber.
Been looking for this recipe, i believe this is the recipe used in the premade jars i bought.
Spore n' Sprout is a great place to learn about fungi growing!🙂
Thanks for the easiest to follow video on liquid culture on youtube. Everyone else makes it super difficult. Or maybe I'm just not a smart fella?! 🤷 lol 😆
I have been looking and looking for a product to buy or recipe for Water Clear Liquid Culture, not yellow... Its just a preference !! So Thank You !! This is the only one I found so far !!
I’m glad it helped! Thank you for watching! 🍄
Thanks!
Amazing ❤
this video was cristal clear! ;D
I've been using this recipe for a out 6 months now, i use a little more peptone and malt extract but it looked like we get the same kind of results, as soon as you showed the ingredients I got excited, this is by far the best LC recipe I've used
It’s really a game changer! I love how clear it is and how fast the mycelium grows compared to just honey. Thanks for watching and Good luck !🍄
Hands down the magic sauce
@@SporenSproutWhat's your process on growing indoor chicken of the woods?
@@IrishEagIe that’s my question as well. So tasty!
I once stored it in cold cooler and it ruined my LC. It wouldnt grow anything anymore.
Can I just keep it in a kitchen cupboard for a few months? Did that only take you 2 weeks to have that much mycelium from just 1 cc of LC injected in that jar?
Thank you for your videos! They are very educational.
I am more familiar with your old videos. Trying to catch up now. I feel my knowledge is a bit rusty after over a year break from studying mycology. I had experimented little and due to health conditions I was forced to have a break and my LC spoiled meanwhile.
I’m ready to restart but now all I have is 1 spore print and 5ml spores suspended on water. Unfortunately I am not able to get more.
I have materials for grains on jars, bags, agar, LC, bulk substrate… What would you recommend I start with so I make the best of the little I have? For grains I have access to rye and popcorn by the way. I am interested in starting a good self sustaining cycle loop so I don’t waste the spores I have.
Your help would be greatly appreciated. Thank you in advance!
I love your videos my friend!!!Have you tried sparging with filtered air?
The video is very easy to follow... thank you bro, from your guidance I made it. As a result, I get mycelium that floats lightly, so clean and smooth like a cloud in a solution that remains clear until the end of the process. Unfortunately I don't understand how to share with friends here. once again, I thank you very much..
No problem! Thanks for your feedback 🙏 I’m glad you like how clear and fluffy the mycelium is with this method.
First of all, thank you for sharing all your knowledge! If a person wanted to run an exhaust fan a few times an hour for short periods of time, do you have any recommendations as far as a timer that is that precise? Thank you again!!
Thank you for making this video, have not tried it yet, but curious how to get the lc out of the jars to inoculate other thing! Thanks inadvance!
You could use this method I designed: Filling Mushroom Syringes with a Peristaltic Pump System
ruclips.net/video/zMVBLV5Cdxo/видео.html
Or just tilt the jar and suck it out with a syringe
LME is the best IMO! Clear and fast growth!
That is awesome I'm going to try it
It’s a game changer!
Thanks for the info
I've been using that same recipe for a couple of years now. It's the best imo. That peptone really makes the mycelium POP.
How do you use those syrups to grow mushrooms
@@emekaalex288 Tht syrup provides food for the mycelium to consume and grow in within the liquid.
That's what a liquid culture is. Then you just draw some up in a syringe and use the LC to inoculate grain.
@@Lulzswag I grap now... Thanks
I got your spores in the mail super fast, thanks. I'm about to make these mixes today, and eventually use them to mass produce shrooms in my 2400sqft shop. Let's goooooo
Nothing better than that 🙌🏼 Good luck brother! 🍄
@@EricCheetoh How to Clone Enoki & Shiitake Mushrooms from the Grocery Store
ruclips.net/video/EARNzNHAzf0/видео.html
12 months!? Wow... trying this immediately
Good afternoon . What do you think about replacing petri dishes and test tubes with glass flat-walled bottles? It is essentially a hybrid of a test tube and a petri dish. The only thing that worries me is the convenience of isolating the mycelium strain in a bottle.
I would like to hear your opinion.
I use this same recipe…it’s the bomb. One gram of each to 600ml water 👍🏼
How is it possible that so little amount of sugar works far better than let's say 4% ?
The Myc actually runs for nutes, instead of being surrounded by tons of sugar. Think it helps expand better @@PPP-on3vl
Do you realise you use about 20 times the amount of what he shows in the video??
@@nelisv7218 nonsense. Other growers verified & I got the recipe from an industrial grower. Had spectacular results & no contam.
Because most sugars like corn or honey you would use at a 4% solution. LME is a complex carbohydrate and can be used at far lower concentrations such as in this video
I have of the ingredients except for malt extract, amy other ingredients to sup the malt? Amd ty
I wonder even grinding down Morning Glory Petals to absorb in with Culture would help with Potency.
I love morning glories!
After trying the original recipe with a 64th of peptone and LME and karo I just put together another 7 quarts of the suggested recipe for more nutrition. The first way shown in this video worked very well. I simply want growth for another round as fast as i can get it and plenty of nutrition to fill the jar with mycelium. The suggested recipe was 1G peptone, 1G LME and half tbsp of honey or corn syrup. I mixed the first quart that way and while stirring it i noticed it was definitely not completely clear. i lowered the LME to between.5 and .75 if a gram, not measuring it exactly just leaving the 1\4 teaspoon less than full and that solved the problem if the slight haze.
Absolutely clear LC with that recipe. 1G peptone which is 1\4 teaspoon, .5 to .75G LME, light malt extract and half tbsp 7ml of Karo. I used karo only because that is what was there and honey seems to caramelize when i use it at 4% I have not tried it at lower concentrations, it might be perfectly fine.
I wanted to list my findings for others who are trying to get completely clear LC but still wanting high nutrition.
Thanks a million for all your help, especially the trick with adding 2 inches of water covering the jars in the bottom of the all American pressure cooker so I don't cause a haze after sterilization. It worked perfectly. I ran it between 12 and 13 psi after getting it to that pressure for 35 to 40 minutes and immediately vented. Perfect results.
Thank you very much!
If you use filtered air through a Luer loc filter disc you will see the mycelium grow in huge leaps and bounds. One in and one out and a 45 dollar aquarium air compressor. I say the 45 dollar one because you will want numerous jars going at one time.
@@Biokemist-o3k
I have all of those parts right now.
I use a blunt tip needle that goes all the way to the bottom of the jar so putting air in that should cause a lot of oxygen going through the liquid and to the bottom of the jar.
Thanks for the idea, I am going to try this method.
@@ZCRAIZED1 Excellent my friend! In Early 2000's I was part of a company in Kennet Square Pa. that had a culture collection that rivaled the National Type Culture Collections. I started cultivating medicinal and gourmet mushroom spawn that we supplied to large mushroom grow houses. With fresh air I had to double the amount of peptone or malt extract because the mycelium grew so fast. We ended up selling the company to Campbell's soup and I suppose it still exists. It was called Mushroom Science Laboratories. keep up the great work.
So the idea is to sterilize the f2ck out of the jar and fill it with some liquid carbs? Would you consider trying a really crude version of this? Like wiping down the jar with some off the shelf isopropyl alcohol and then dumping some sugar water in with some innoculant? Idk I think it'd be cool to see if it works. Anyway, really cool footage.
It looks like a lava lamp❤
What flow hood are you using? Does it work well?
I didn't see this and as usual I mixed everything in hot water, the peptone took a small amount of agitation to get it mixed but all three ingredients were very easy to mix in hot water. LME and I'm using light Karo just because that's what is here. I have honey but at 4% it badly clouded and a lot of sediment. I'm guessing I caramelized it with too much heat and too long of a temperature.
Hi, I appreciate your concise and simple instructions to create liquid culture. What is the suggested time to let the culture grow mycelium or is it by sight? (i.e. 50% of the liquid has mycelium) I'm trying to build up my culture backlog and extend my spore purchases. Thank you!
Yes I’d shoot for 50% and it usually takes 1-2 months
Thanks for the valuable content. What amount of dextrose can be added instead of corn syrup?
1/2 tablespoon of dextrose
i love the clearness and very fast growth of this recipe. thank you! however, most of my strains now start to make significant rafts. i first thought it was contam, but surely they are not all suddenly contaminated. it gathers up in big floaters on top that is very hard to break. have you experienced the same?
Yes I experience the same, You have to stir frequently to prevent the mycelium from doing that and becoming too thick. You can switch to honey LC if it becomes annoying. Or you can watch my blender video where I blend the LC: ruclips.net/video/LGvAu0ux-VI/видео.htmlsi=Or-S6wzPFjK0Y62v
@@SporenSprout i was thinking if they get a bit too much nutrition still? have a feeling it could be related. not feeling super comfortable with these rafts, and the additional step of blending i would also like to avoid. what do you reckon? i'm coming from honey LC and that's been working fine, but i feel the clarity of this method makes it easier to spot bacterial contam.
can you please do a conversion to grams and milliliters?
I second this
This might be a dumb question but I'm a newbie so please don't make fun of me lol. Is it possible to take liquid culture that has already been formed, and inoculate another jar with it to allow it to grow? Essentially can you use one jar to make more jars?
Also, on a side note, that is some absolutely beautiful looking liquid culture you have. Nice work! 👏👏👏🙌❤️
Yes you only need 1-2 ml of liquid culture to make a whole new jar and you can keep doing that for a while.
How much growth do you get after a week? Is the one you show with that much grow only after 7 days?
Thank you for this recipe. What do you think about using powdered corn syrup? Does mycelium have a preference of bone peptone over soy, or vice versa?
Do you feel that this broth will work for pestilotiopsis microspora, initially anyway?
We are excited to find out how this works with all of our different species. We have a number of projects going on both professionally and personally that this may be a nice addtion too.
Thanks again for sharing it with is all!
I’d go with soy over bone. The bone peptone makes the mycelium grow on the surface too much and also smells bad. Yes I think it will work great for any microorganisms really. I have never heard of corn syrup powder but for me corn syrup is never strong enough. I like honey.
when it is ready to fruit what do i use for the substrate do i have to use grain and inject it or can i sterilize a plastic bin with cardboard or something else please let me know
Make some grain spawn: How to Make Sterilized Rye Grain Spawn for Growing Mushrooms
ruclips.net/video/uFQr2JhqmG4/видео.html
😂 I just realized I brought liquid culture from you lol
That’s awesome! 😆 Thanks for the business and for watching! 🍄 Good luck!
Is this better than your honey recipe for Cordyceps Militaris? I ordered some syringes from you last week and plan on making the LC before they get here. Thanks. Love your videos.
It works well but if you don’t stir it everyday the mycelium tends to clump and tangle which can be annoying. I prefer honey LC for that reason.
@@SporenSprout I do have a stir plate if the LC is better suited for propagating the Cordyceps. Thank you!
My presto states to fill 3 quarts of water when using it.. does the LC recipe glass mason jar have to sit completely above the water during the 20 minutes or is it ok if the bottom portion of the mason jar is submerged in the water?
An inch or 2 covering the bottom of the jars is normal. You can use jar lid rings to help raise the bottom rack further if needed.
If I have light spore specs still floating do I keep it in inoculation longer and weight it out ?
Yeah. Next time germinate the spores on agar first.
Great video and thanks. Thats 1/64 tablespoon. A teaspoon is about 1/8 of a tablespoon. So 4x that small one you got there or 1/4 of the big one (the size of a nail) would be a teaspoon.
Why does the spoon say “1/64th TSP” right on it then? It would say TBS if it was 1/64th tablespoon. Either way it doesn’t matter, you can use more nutrients than that. The only reason I used such small amounts was because I had sediment but I found out it was from not using enough water in the pressure cooker. Make sure the water level is touching the bottom 2” of the jars while pressure cooking.
@@SporenSproutthe spoon is wrong :) Americans generally have a different understanding of teaspoons they are really tiny in the rest of the world. I like your content just wanted to point that out
Thanks to these LC videos promoting this bacteriological peptone, Amazon is sold out. I decided to order the soy peptone instead.
Given these results from something sooo diluted, we all need to promote this recipe so there's more peptone to go around as this mycological revolution ramps up 😉.
Good to know at least that LC solutions can be successful over such a broad range of concentrations. I don't think I'll even bother measuring after watching this; just a couple of pinches of each to be sure I have at least this amount, but obviously waaay less than other recipes I've seen.
Is there a reason you stirred with a frother initially instead of just using the magnetic stir bar?
Doesn’t matter really, just use whatever you have to stir it at the beginning.
Hi what an informative video. thanks, I am making mine to your recipe and need to know what temperature to store the jar at?
You can store them at 70-75 F. When mixing the ingredients you should start with room temperature water. The ingredients do not dissolve well in hot water. You can use 1/4 teaspoon (1 gram) of each powder as long as the water is not hot. It should dissolve perfectly in cold/room temp water. It also helps to reduce PSI from 15 to 13-14 to prevent opaque cloudy LC media. Good luck!
@@SporenSprout thank you for your detailed response
I wish I would have seen this video last night. I only saw the short one and didn't catch the statement about watch the full video. I made 7 quarts of the lighter nutrient content. I'll be making this heavier recipe tonight if I can figure out what to do with my all American canner to get it to not make the mixture clouded. I'll definitely take your advice about running it at less than 15 PSI.
Thanks for everything, cheers.
I didn't see this and as usual I mixed everything in hot water, the peptone took a small amount of agitation to get it mixed but all three ingredients were very easy to mix in hot water. LME and I'm using light Karo just because that's what is here. I have honey but at 4% it badly clouded and a lot of sediment. I'm guessing I caramelized it with too much heat and too long of a temperature.
ive had cultures last 3 years in a dark cool closet..........dont disturb the water wall inside and the mycelium will give off a digestion enzyme that sterilizes the water and essentially puts it in stasis.
Hello! First of all - amazing video and results! I would use some advice if you don't mind: I'm using 8 grams of fructose in 200 ml of distilled water. Sterilized the solution and inoculated it, kept the jar wrapped in the aluminium foil at 24-26 Celsius. It's been 4 days now and the only thing that appeared in the solution is a very thin and short transparent (or white, it's hard too tell they are so tiny) strings of supposedly mycelium. Also the spores (they appear as very dark brown dust) are doubled in size.
Is it going well and I should proceed with waiting or the result I have now (4 days) is not enough and I should just scrap it and start all over again?
Thank you in advance!
It might be ok but spores are not very clean to begin with so it’s not adviced to inoculate with spores. You should germinate the spores on agar first and then transfer to LC media. You will need to check the liquid you made on agar to know for sure if it’s good
@@SporenSprout gotcha, thanks for the answer!
Love this! ❤ But serious question : What kind of needle do you use to get to the bottom of this? Or do you fill the syringes with the lid opened in a sterile environment? Thanks!!
I tilt the jar and use a regular 1.5 inch needle.
Tilt the jar
No u tip the jar or u can get a longer needle but when doing so run risk of letting something bad in and ruining ur culters
I wish you sold these jars on your website !!!! Lol I live with other people and can not make these, plus I do not have a pressure cooker... :(
can boil in a pot (with lid, creating steam) for 90mins. make sure the water is a couple inches above the jar and u put some sort of rack on the bottom of the pot.
Is this sterilisation is 30 minutes at 15psi? .should we Weight until the pressure cooker turns to 15 psi and the 30 minutes or all together 30 minutes from the minutes I started pressure cooking?
Yes 30 mins once it gets to 15 psi.
Question: I followed this recipe but I forgot to tighten the lids after I took them out of the pressure cooker. I left the foil on top though. I took the foil off 3.5 hours after removing them from the pressure cooker and noticed the lids were loose so I tightened them at that point. Do you think the jars could be contaminated? Let me know if you think I should start over.
You’ll most likely be fine to use them
I tried this exact recipe which looked amazing and the only thing I didn't do was use your brand of malt. The corn syrup and the pep tone was the same brand from Amazon. I used Anthony's malt and it will not dissolve. Right now I'm not sure if it's the malt or the peptone but something isn't dissolving no matter what I do with the magnetic stir. They're the same color so it's hard to tell. And guess my only other avenue is eliminating one and then the other to see if they work at dissolving entirely in the liquid
Increase the amount of water in the pressure cooker. The water should be touching the bottom 2 inches of the jars while pressure cooking.
I did a jar without the Anthony's malt and it dissolve fine with the magnetic stir before pressure cooking. I'll try your suggestion on that with the one that still has Anthony's malt in it
great recipe, can i replace syrup with dextrose?
Yes
Great vids, thanks, you would shoot spores into this ?
I’d rather make sure the spores are clean on agar first but yeah.
So I didn’t use an agar plate, but I haven’t used my spore syringe yet, so I still have time to buy one - how would I transfer it from agar plate to liquid culture ?
It's very easy if you haven't already figured it out. You just cut the area of agar that you want and drop it in the jar.
I have a question . Do you burp your sterilizer , or simply allow it to run for 10 minutes before shutting the valve ?
I just let it vent for 10 minutes and then shut valve
You can leave it out in a cool room? Cool like like 65 to 70° ?
70-75 is best but it will just grow slower at colder temps.
What heat and light conditions do you sore the LC jar at immediately after inoculation?
Light doesn’t matter, temp set at 70-75 F
@@SporenSprout Thanks man.
Thanks so much, is it possible to convert the tea and tablespoon measurements into grams per 600ml?
55 mg of each powder and 20 grams of syrup.
@@pouyarezaei5976 Yes, you can try adding more but if it produces sediment than put 55 mg of each.
Would using the mycelium in here be ok to use in an extract? Instead of like using the mycelium from rice?
Yes this is a great way to harvest mycelium without grain filler.
What do you think about adding brewers yeast? Ive seen other recipies with this but im not sure what it does.
Question so Ive seen online that people have said you only need a few drops of a spore syringe to 1ml. So what is the truth can you get away with less ? Or do you need more spores
A spore syringe is not perfectly clean so you should only use 1 ml. If you germinate and clean the spores on agar and make liquid culture from it than you can use 2-5 ml
❤❤❤
If myc appears flat floating at the top or sunk to the bottom is it dead?
It is normal for the mycelium to grow on the surface and at the bottom.
After you insert the syringes do you put the jars in the refrigerator?
No, after inoculating the jar it is allowed to grow at 75 F and once it’s full of mycelium you can put in the refrigerator but I prefer to just move them to an air conditioned room instead
This week for starting mycelium grow I need to keep it in dark, worm place?
Yes that’s ok. Light is not a big problem
I dont have light malt extract but can i use only peptone and syrup?
Yes that would be ok. Or use just 1 TBS of honey.
nice tattoo. greeeeeeeat video brooooooooooo.
Could you pitch that jar into sterilized and start growing?
if I'm using half gallon mason jar should I still PC at 15 psi for 30 mins or go longer than 30 mins? also if using spores syringe, how many cc should I use for the half gallon jar if I need fast colonization?
30 mins is still fine. 1-2 ccs although spores are not guaranteed clean. Best to germinate on agar first and then transfer to liquid. You can do it though, just make sure to test a drop of the final product on agar.
@@SporenSprout doing agar currently is not possible, I got those spore syringes from a very reputable supplier that I had successful spores to grain 10 years ago from their syringes, I'm also following a very strict hygiene including sterilizing neddles in PC and inoculating in well sanitized closed area and using self healing port like the one in video and for the GE I'm using 4× 1/4 inch holes and on each hole I'm using tyvek and on top of it GE filter disk..
@@mo3taz_mizo38 You should be fine but you can easily buy pre made agar plates here if needed: amzn.to/42C7ZrX
How long/often should LC be put on the magnetic stirrer?
5 minutes every other day is good.
@@SporenSprout I appreciate the info!
Karo vs corn syrup ? Or what’s the difference ? Thanks in advance love the videos !
Not much different other than the karo most of the time has vanilla flavor added to it
I read the ingredients and saw both types of Karo have vanilla. I wondered if that made any difference at all. I was concerned it might caramelize but I had no issue.
Does this work well for any dung loving mycelium?
Yes
I am not capable of fully dissolve the ingridient and still look cloudy, any suggestion?
I am using lme, bovine bone peptone and glucose syrup ( didn’t find corn syrup where I live)
Make sure the water in the pressure cooker is touching the bottom 2” of the jars while cooking.
@@SporenSprout I will try , thank you
Is time for growth the same regardless of what mushroom you’re growing? For example I thought Cordyceps were ready after like 4 days tops?
With this recipe the mycelium initially grows faster but will not completely fill the jar like other recipes with more nutrients like honey. Cordyceps can grow for 1-2 weeks
Once you got the mycelium grown and stirred you can store it for 12 months untouched or do you still stir it daily?
You can stir it once every 1-2 weeks at that point.
Do I need a vent along with the self healing port?
Yes I use 2 layers of micropore tape.
Someone please tell me what the red injection port stuff is called
RTV silicone: amzn.to/4aFLVQ8
Is boiling tap water sufficient for this method or have you got any advice on how to distil water at home. Having a terrible time getting LC to work with honey currently so definitely going to give this a go!
If you are having trouble making the honey LC media. I recommend making sure the water in the pressure cooker is touching the bottom 2 inches of the LC jars. If you had the water level barely touching the jars or not at all then that was your problem.
@@SporenSproutwill definitely do this. I notice your water is steaming before you mix in the ingredients. Would boiling it be ok prior to sterilising?
@@robinsimpson6654 use hot water but not boiling.
Just curious, could you explain why this Recipe works with just a fraction of the nutrients vs all of the other ones that use grams of this stuff?
The low nutrients force the mycelium to quickly expand in search of more food but won’t grow as full. You can use more nutrients and that’s fine. I just had problems with cloudy media when using lots of nutrients but then I found out that my all American sterilizer is what was causing the cloudiness so now I only use my presto 23 quart pressure cooker for LC media.
Great video, like always!
Is there a substitute for the peptone? I wanna try this recipe but in my country is not that easy/cheap to get!
Beef extract or vegetable extract.
@SporenSprout oh you can use beef extract? That's really cheap and I have it in my pantry. Peptone is ridiculously expensive in tye UK but beef extract is like 3 pounds
hydrolyzed collagen bovine unflavored
Would vegemite. Work?
@@SporenSprout if there is much salt in it, is that a problem?
My first LCs (3 jars of golden oyster, and 3 jars of lions mane) are 2 weeks old now and seem to have stalled. The golden oyster blob rises about 1/3 of the way to the surface, and lions mane is only about 1/5 or 1/4. This video inspired me to try a milder solution than most LCs recipes you find, although by my math, my recipe is still 5.5x richer than this (1/16th tsp each of DME and soy peptone per 350 mL jar). I'm also running 2 jars with a sloppy half teaspoon of honey added to make note of any differences, and so far there aren't any.
Another difference is the fact I'm using a 0.22 micron syringe filter installed in the lid to provide the FAE. Do you suppose the syringe filter provides inadequate FAE for a 350 mL liquid culture in a 500 mL jar? I've given them a vigorous stir twice now. Do you think perhaps I need to stir more frequently, or for a longer duration? Or perhaps I'm just being impatient. What level of growth should I be seeing at 2 weeks?
Just impatient to be honest, it takes time man
Can I use Jam jar for LC?
Yes you could
can i use water from any machine ( water dispenser )
Yes
can i store it in the fridge or should it just be at room temp. After the mycelium has covered the jar and you want to store it.
After it has grown enough mycelium you can store the jar in the fridge or in a cold room.
@@SporenSprout ty. nice vid btw
Is that filter tape on the lid or is it completely sealed with the exception of the injection port
The lid has 2 holes. 1 hole has RTV injection port and the other hole is covered with 2 layers of micropore tape.
@@SporenSprout awesome! I like it
Hey amazing video!!! Quick question: does this recipe works with lion's mane mushrooms?
Yes it works for all species
@@SporenSprout can I replace malt extract with malt extract agar?
@@hungnamnguyen9003 no because it will become a solid instead of liquid due to the agar.
@@SporenSproutThank you so much for relying to me!! But is there a catch for this method? Is there a limited time you can do this?
I also watched your video on grain to grain transfer. Is there a limited times you can do that also?
@@hungnamnguyen9003 you certainly can’t do it forever. After 1-2 years you will need to start again from spores which is pretty easy anyway.
Can we use it for agar recipe?
Yes I just use LME for agar
Which model pressure cooker are you using? The 921, or 925?
I have the 41 quart, 75x electric all American
@@SporenSprout omg, that has to be a beast! Does that run on 220?
@@RipperRUclipsOfficial yeah I love it! 120 volt
I've been using a recipe that uses about 1/2 tsp peptone and 1/4 tsp LME, but the same amount of corn syrup and water. You've found these much smaller amounts of peptone and LME to be superior to larger amounts like that?
Yes in my experience the smaller amounts work better. If you can fit more in without turning the solution cloudy than keep doing that. If there is any cloudiness the mycelium will be initially inhibited in my experience. I’m thinking a hot plate will allow you to dissolve more without it compromising clarity.
@@SporenSprout Interesting. I'll definitely try this out.
Couple of questions. What happens if say 10% LME is used? and 2. How does the amount of LME affect the long term shelf life of LQ?
@@jobbingactor you can use more ingredients as long as you make sure the water level in the pressure cooker is touching the bottom 2” of the jars while sterilizing. The amount of nutrients does not have a big effect on shelf life.
@@SporenSprout Credit where its due your one of a tiny few you-tubers that takes time out there day to post replies and on top of that, very quickly. It doesn't go un- noticed. Message received. Ive prepped some jars very similar to yours, I had Malt yeast extract but no peptone so went for a gram of yeast nutrient. Yesterday's jars were clocking on for 20g per litre so I watered those down with distilled water to about 5% and will PC them again.
So with this once you on your last syringe could you just repeat this process all over again?
Yes
how much in weight is it 1/64 spoons of DME and peptone?
Just use a gram of LME and .5 gram peptone and make sure to use presto 23 quart with 3 liters of water in it. I used low ingredients in this video because my all American pressure cooker always ruined my media by making it cloudy and I didn’t realize that for a long time.
I use the same ratio of peptone but it always turns my LC yellowish.
That’s ok 👍
what is the alternative when i dont have peptone? peptone is expensive in my country
Beef extract or vegetable extract
Questions about stirring: You say to stir once at the end and then let sit for a week. Then stir everyday after that so it doesn't form at the surface? How many days do you stir until? When do you put it in the cold room? Do you stir once it's in the cold room?
Just stir every other day for a couple weeks and then stir once per week once it’s full and in a cold room.
@@SporenSprout Thanks! And could a cold room be a fridge?
@@eliotube Yes a fridge is good.
@@SporenSprout About how long does it take for the jar to be fully colonized before it goes into the fridge?
@@eliotube2-3 months