The links to the parts are serious time savers, and the knowledge to build these extraction lids myself is a serious money saver. You have done us all such a great service with the concise, excellent little video. Thank you very much!
I'm glad someone finally covered this. I'm in the process of doing this in a couple days. I was wondering if all the parts were autoclavable. Thanks for finding out for me. I plan on using a one-way check valve instead of the stop-cock. Until now I used 8 gauge metal dispensing needles, but epoxied them to the lid so they couldn't spin or move. I also use UV glue wherever I can. Keep making great videos, we need them.
Yeah, well it was kind of crazy because while it was chugging along at 15 psi, one of those red caps popped off of 1/4 LC jars I made and it was shootin steam out the top of the jar hose so much so that it made the pressure force It's way out the weight valve I almost jumped out of my seat because I'm a quadriplegic 😂but that one jar it happened to was like 150 mL low so it was like a mini little pressure cooker, but inside of a big pressure cooker 😱😱
@@goodtoknow1331 any time you open the jar after it has been sterilized you are massivly risking contamination. Even in front of a flow hood. Also if you use regular lids (i assume you mean regular canning lids) they will be vacuum sealed to the jars. That requires a bit of prying to get off. The whole point is to never have to open them after thye have been sterilized. I thnk that is why he also has the inection port so that he can use it to inoculate. If you used the tube to inoculate you would probably have to flush the tube out (down into the jar) with sterile water in order to get the inoculant MYC down into the nutrient liquid. Otherwise it would only be in the tube.
Great Video Very Good Idea I am growiing in the tropics where contamination is a huge problem I will try that solution. One question though. Is it possible to also inoculate the LC through that silicon hose? Or will too much stuff get stuck in the hose?
I made Some last week and I was putting aluminum foil over the draw a tube cuz I couldn't confirm that the red tips were autoclavable.. Thank you so much! I'm really loving your style of content!
So for me, when my jar is ready to extract I prefer to pull it all in one sitting. Once the mycelium has colonized the jar to my liking I will empty it into as many syringes as it will fill. If you're only filling a couple you can try to sterilize with ISO, or you just put a fresh sterile stopcock on each time (which will cost more down the road).
super helpful Content. i have 4"x14g Stainless needles im currently using for my LC, and this is a great way to not need sharps while also being able to reach all of the LC in the jar with less flame sterilization. can just buy blunt fill needles in bulk for inoculating Grain
Ir seems that tracking all the items down in the UK is a bit more difficult, primarily the female barb adapter. Is it possible its named something other that stated?
I'm very new, and have had plenty of "learning opportunities" already😂 Just this weekend i worked with lc jars for my first time (i did inoculate them myself, but getting it out of the jar... oof) I made plenty of mistakes! These will address SO MUCH of the challenges i had. Thanks!
@RealSimpleMushrooms Thanks mush! One thing I do have is stubborn tenacity (or aggravating bull-headedness, depending on who's asked 😈.) (Left a new question for you, just in case it's something others would find helpful.) ❤🍄❤
I was having trouble sourcing female luer to 1/4 barb adapters available on Amzn that were polypropylene. I opted with male adapters which will still connect with a 3 way stopcock and leave 1 connection available for a syringe at the stopcock. Throwing this solution out there to anyone having supply issues with the female adapters rn.
If you're inoculating from another LC it would work just fine. You could also sterilize some water, draw some into a sterile syinge and then use the needle to basically biopsy an agar plate then inject as well. Just need enough of a sterile carrier fluid so that some mycelium makes it all the way down the pickup tube.
Hi. I really appreciate this video, it is very useful information. Wondering if you can use a magnetic stir bar and plate or if the silicone hose will interfere with the stir bar? Thanks
I use the injection port if I have a known clean LC I want to expand.....or if I just want to draw one syringe to agar test. Once you use the stopcock it's no longer sterile. Me, I will empty the LC jar into syringes in one sitting.
Found everything but the pp5 lid, great video, I'm going to try this out . Thanks. Amazon does have brass and stainless steel stop cocks. I'll use the plastic ones I think though
Best practice is these are one time use…for me anyway. That’s why I just empty the jar in one sitting and store the syringes in the fridge until I use them
Thanks so much. Nice work! If you see this…what heat sealer are you using & do you like it? I’ve had two now, and neither one seals the whole way thru all layers of my bags!!
@@RealSimpleMushrooms Awesome! Will that filter patch suffice for air exchange? A friend of mine told me to never fully seal jars because its probable it can burst in the PC. I'm wondering if the filter patch mitigates that risk
Is the stopcock necessary? What if you just took the red luer lock off and hooked the syringe straight to it without the stopcock? Also, how is the stopcock cleaned and used again for next jar of LC? Is this a one time use? Since you cant autoclave it, how do they get sterilized?
You did not list the injection ports in the materials and you did list syringe filters but did not go over anything about them in the video but had the jar with them on one. So exactly why did I purchase them if I'm not using them and you didn't show what they were for?
So I use the injection ports for 2 reasons. 1. in case I want to inoculate the LC jar with a current syringe I know it viable and clean 2. Sometimes I will pull a quick sample through it to agar test it for contam.
Hi I’ve been using these lids a couple times now and they’re the dream. Just wondering if it would still be sterile to refeed through liquid culture through the luer lock with PC liquid culture solution syringes? Or have you already tried this?
Very interesting, does that stopcock leak over time? That'd be my only concern because if it doesn't stay air tight at all times gravity will pull the liquid down and draw in dirty air
So for me I will just empty the entire jar into syringes in one sitting, but I also sell cultures. I'll store them in the fridge to slow the growth but they last upwards of a year in the syringe as well
Instead of home made jars, you can use dextrose water 5% serum, medical grade sterile, with self healing injection port, and use serum set for injecting and aspirating, you could buy from a pharmacy
You're talking about iv saline bags. Yes? I can see that being effective and easy. I would prefer to leave that much medical waste for medical purposes, though, if possible. There's also the cost and the volume. I'm not going to need THAT much culture for any one strain, not for a LOOOONG time, anyway. I'd also have to figure out storage. Combine that with the cost per bag (that I've found so far, anyway) and unless I have a family member with "sticky fingers" working in a hospital setting (and I was willing to do that), that could get pretty costly kinda quick. I'll try these reusable forms. I'll keep in mind the bags as a backup, though. I can actually see them being very organized and efficient in a larger, professional setting even. For a home or small-scale mycology enthusiast, though, I think these will tend to be the better option overall. I also will be trying to minimize how much landfill I produce per "grow" where and when I can. All that said, if you have sources for smaller bags or for what you see as more cost effective, please let me know? I'd appreciate it! Good thoughts. Thanks! Agapé 303
No, I would just take up some sterile water in a syringe, then use the needle to biopsy the mycelium, (push the needle into the agar which should pick up enough mycelium) then just use the sterile water to push the myc into the jar.....does that make sense?@@KOKO-uu7yd
@@KOKO-uu7yd fyi, 5%dextrose water bags, not salin ones, they come in a verity volumes, 300ml up to 1000ml, these sugar bags are relatively cheep, sterile, equipped with self healing injection port, easy to buy from a drugstore , mostly come with sterile serum set which could coupled with syringes and cross connectors for easy usage, could be stored in cabinets for long periods of time, could save time and energy compared to home made sugar jars, with much much less risk of failure/contams. On the other hand, you may get satisfied if you make your own liquid jar while doing mycology as a hobby at home.
@@RealSimpleMushrooms It does, i think, for my other posted question? I'm commenting so much im getting confusing, i think! 😂 So yeah, I think you're talking about using the cockstop to inoculate? If i understand correctly, something already liquid should be fine. If I'm working with something like agar or spore prints, I'll still need to have a "carrier" of sterile solution. Other than that, I should keep itnsimple and not over think. I got thst all about right?😂 Also, THANKS! You've taken some considerable time and responded quickly, to boot! Mighty kind😊😊😊😊😊
I bought everything in the description and did everything according to this video… Everything went great, except for the little red cap with the Luer lock melted and had aluminum foil covering as well, so what did I do wrong? 😂
No, you didn’t do anything wrong. Those Lids seem to be a little hit or miss. I’ve had some survive and I’ve had some melt, I’m looking for a better solution now.
@@RealSimpleMushrooms there are nylon and PP ones on McMaster. Did you have problems with contamination after the plugs melted? Or should I be good to just swap the caps out while I wait for new ones to come?
First, this was a great video.. THANK YOU ! Second, I just did the same and had the same issue with the red cap. I went back to Amazon and read the reviews, and older reviews didn't mention an issue with the caps but more recent ones did. I had just ordered new caps from a lab supply company and will reprocessed the jars when they are delivered. Not the end of the world , just annoying.
very neat, But aren't you risking contamination more by having to take off that red cap and putting the valve on? And then having to take off the valve cap and putting the syringe on and off ? I love the tubes though. If there was a way to combine an injection port with the tubes (not seperate on the same lid, but together as a single line) I think that would be close to ideal. Have you ever tried that? I aslo dont think you really need high temp RTV as that is usually for the exhaust ares of engines. Regular silicone should be able to handle 250°F easy. Have you tried it and had failures? LOve you videos clear consice simple, very nice. Thanks. Also any reason to use a 3-way valve when 2-way are cheaper and just as easy to get. And why even use the valve if you cannot sterilize it? Use it once then trash it? I am just trying to understand as I am currently building up my LC cultures and plan to have about 20 jars, and will use at least a full jar a week. for inoculating grain jars
You're correct, any changes in open air you risk contam. Just be sure to do that in an SAB or in front of a flow hood. There are stainless stopcocks out there but they are quite pricey at over 100 buck or more each, but those would be ideal as you could then just PC the entire setup......AND as it turns out those red PP5 caps melt anyway. I made some updates to the description with more information and links.
What is the correct size drill bit for this? I ordered the one from the list above and it's a huge drillbit that is much bigger than yours.. just letting everyone know and wondering which is the exact size to order? Thank You
I only used the 3 way because they were readily available on Amazon. The only reason I wouldn’t connect it right to the female on the bulkhead is storage if you don’t pull all your LC into syringes in one sitting. The stopcock gives you the ability to close it up and store it without worrying about contamination
@@RealSimpleMushrooms once closed up and stored to prevent contamination, will a new stopcock need to be installed if you wanted to use the culture on more than one occasion?
Question for you (I'm rewatching now) - you have an injection port, but could one inoculate with lc or spore syringe via the stopcock? Perhaps a flush of sterile H²O would be needed to get everything down the tube and into the medium... 🤔🤷♀️ I'm not sure of this would ve an advantage or not, or even if it would work? Your thoughts? Thanks again!
@@RealSimpleMushrooms yeah I’ve analysed your methodology here and it’s really well thought through. With the 6mm ID on the silicone tubing it’s evening amazingly well suited for SSLC, take a peep if you haven’t already come accross it ;)
Those pp5 lids are not pressure cook safe, I just built this exact setup 25 minutes in the pressure cooker deformed and melted the lid. Gotta buy another pack now😢 other than that I’m excited to try out once I get some more
@@RealSimpleMushrooms mine were so melted it was a major pain to get them off and replace them. Hoping I dont get contam as I replaced them while on sterilized jars
Yes I use stir bars, the hose usually has a small curve to it and you can move the jar slightly when stirring to avoid hitting the pickup tube. They work just fine
@@RealSimpleMushrooms amazing man thanks for the tip. Excellent video and thank you for your prompt response. I am in the middle of expanding my LCs and definitely ordering the items from your comprehensive list thank you for taking the time to do that I really appreciate it.
So after I build them I’ll run them through my dishwasher on the sanitize setting then I will put them in a mushroom grain bag and PC them for 60 min at 15psi. I will leave them in the bag until I’m ready to use them.
Soooo Sorry, I've had a stroke, and I'm still stroke stoopid. :-( Thank you. I see it, should have looked there and I thank you for shared knowledge. Stroke cause me to experience the near death experience, but also to hallucinate for about 4.5 years. I discovered mrooms in my quest to answer, 'what the hell did I see?' Fortunately, mrooms did not reproduce the quality of graphics that I saw. DMT did not produce the quality of graphics I saw. Nor did acid. Salvia Divinorum came closest, and scared the daylights out of me multiple times. However, SD nearly duplicated the quality of the graphics of my near death experience, but I saw unrealistic devices and mechanisms. There was none of that in what I identify as penducular hallusinosis. I did lay prostate while a small man put his hand inside of my head. He also pulled the nerves in my head such that my eye was outturned after the hallucination 😲. if you want more of these kinds of narratives but with far more detail including being kidnapped and charged with very strange crimes, you might enjoy, I mean appreciate 'The Mysterious Red Light Camera Murder' I'm looking for a publisher, and prissy literary agents are objecting to the use of curse words and low-life expressions such as 'Cops were trying to inventory my dingle berries.' By far, mrooms were the most prosocial of all the things I mentioned. @@RealSimpleMushrooms
now that weve covered the basic stuff, how about we get more in depth and talk about recipes for cultures, good substrates for mushrooms and how different species have different needs. I've heard you can just use honey and water as a liquid culture recipe.
Your information is amazing! Thanks so much for your information especially where to get the items on Amazon. My question is this: if you don't want to use up all of the liquid culture, can you put a cap on top of the stopcock, after using 70% isopropyl alcohol to sterilize it? It seemss that even if you use a new, sterile stopcock, wouldn't it still have possible contamination when you remove the previous stopcock? Thanks. PS) I think you should patent it! I've seen the Myco lid using a metal transferring needle instead of using your silicone tubing, and your lids are superior!
As long as your sterile tek is good you should have no problem using the stockcock more than once. What i generally like to do is have a small jar with 70% iso in it and I'll just drop the caps in there while I'm filling, then wipe them down and put them back in when I'm done. It's been working great for me.
Hello my friend. I am an experimental mushroom researcher from Iran. I found much simpler ways than you, which increases the speed of your work in the production of mushrooms, especially in the stage of making agar plates and sowing seeds, as well as in the production of mushrooms. These methods are used for both types of food and medicine and magic. I have many achievements in this field that are rarely mentioned anywhere. For example, the effect of the type of seed on the fertility and the creation of mushroom contamination, or the formation of hybrid and hybrid breeds, I mean the integration of mushroom breeds with each other and the creation of breeds that did not exist until now.I think I am creative and thoughtful in this field and I have a personality to communicate. I am looking for friends like me who are curious like me. Let's learn and teach from them
![Coco Coir vs. Dino Soil: EPIC Mushroom Substrate Showdown [Part 2] - Day 12 Update](http://i.ytimg.com/vi/Vsf2MqIQIQs/mqdefault.jpg)
![Coco Coir vs. Dino Soil: EPIC Mushroom Substrate Showdown [Part 2] - Day 12 Update](/img/tr.png)
The links to the parts are serious time savers, and the knowledge to build these extraction lids myself is a serious money saver. You have done us all such a great service with the concise, excellent little video. Thank you very much!
I'm glad someone finally covered this. I'm in the process of doing this in a couple days. I was wondering if all the parts were autoclavable. Thanks for finding out for me. I plan on using a one-way check valve instead of the stop-cock. Until now I used 8 gauge metal dispensing needles, but epoxied them to the lid so they couldn't spin or move. I also use UV glue wherever I can. Keep making great videos, we need them.
Hahaha, only one way to find out what works in the PC right....lol Glad you're finding the videos helpful. I do enjoy making them.
Yeah, well it was kind of crazy because while it was chugging along at 15 psi, one of those red caps popped off of 1/4 LC jars I made and it was shootin steam out the top of the jar hose so much so that it made the pressure force It's way out the weight valve I almost jumped out of my seat because I'm a quadriplegic 😂but that one jar it happened to was like 150 mL low so it was like a mini little pressure cooker, but inside of a big pressure cooker 😱😱
Why not just use regular lids during the PC and then swap them out for these in front of the laminar flow hood after ?
@@goodtoknow1331 any time you open the jar after it has been sterilized you are massivly risking contamination. Even in front of a flow hood. Also if you use regular lids (i assume you mean regular canning lids) they will be vacuum sealed to the jars. That requires a bit of prying to get off. The whole point is to never have to open them after thye have been sterilized. I thnk that is why he also has the inection port so that he can use it to inoculate. If you used the tube to inoculate you would probably have to flush the tube out (down into the jar) with sterile water in order to get the inoculant MYC down into the nutrient liquid. Otherwise it would only be in the tube.
This is that 2024 content that we need. Liked and subscribed, thank you so mush! Mush love 🍄
Thanks for the sub!
Great Video Very Good Idea I am growiing in the tropics where contamination is a huge problem I will try that solution. One question though. Is it possible to also inoculate the LC through that silicon hose? Or will too much stuff get stuck in the hose?
I made Some last week and I was putting aluminum foil over the draw a tube cuz I couldn't confirm that the red tips were autoclavable.. Thank you so much! I'm really loving your style of content!
Glad I could help
@RealSimpleMushrooms do I need to sterilize the valve with alcohol after each use ?
Could u pls show this how u handle it
So for me, when my jar is ready to extract I prefer to pull it all in one sitting. Once the mycelium has colonized the jar to my liking I will empty it into as many syringes as it will fill. If you're only filling a couple you can try to sterilize with ISO, or you just put a fresh sterile stopcock on each time (which will cost more down the road).
Thanks for doing this, so hard to find quality instruction on how to make these lids
Glad it was helpful! They're so easy to build, and people are out here charging $15-$20 per lid and they aren't even as nice as these lol
Your videos are a huge help. Thanks!
Glad to hear it! Happy to help!
Ordering all this stuff right 😸 meow! Thanks for all the info
Any time!
super helpful Content. i have 4"x14g Stainless needles im currently using for my LC, and this is a great way to not need sharps while also being able to reach all of the LC in the jar with less flame sterilization. can just buy blunt fill needles in bulk for inoculating Grain
Glad it was helpful!
do you have a link for those needles? They are what im looking for.
Ir seems that tracking all the items down in the UK is a bit more difficult, primarily the female barb adapter. Is it possible its named something other that stated?
Beooooooo I was just wanting a tutorial on this exact thing!
Awesome! I love these lids
This was extremely impressive.
I'm very new, and have had plenty of "learning opportunities" already😂
Just this weekend i worked with lc jars for my first time (i did inoculate them myself, but getting it out of the jar... oof)
I made plenty of mistakes! These will address SO MUCH of the challenges i had.
Thanks!
You got this!
@RealSimpleMushrooms Thanks mush! One thing I do have is stubborn tenacity (or aggravating bull-headedness, depending on who's asked 😈.)
(Left a new question for you, just in case it's something others would find helpful.)
❤🍄❤
I was having trouble sourcing female luer to 1/4 barb adapters available on Amzn that were polypropylene. I opted with male adapters which will still connect with a 3 way stopcock and leave 1 connection available for a syringe at the stopcock. Throwing this solution out there to anyone having supply issues with the female adapters rn.
Great video but quick question, how do you think inoculating through the stopcock instead of through the injection port would go?
If you're inoculating from another LC it would work just fine. You could also sterilize some water, draw some into a sterile syinge and then use the needle to basically biopsy an agar plate then inject as well. Just need enough of a sterile carrier fluid so that some mycelium makes it all the way down the pickup tube.
@@RealSimpleMushrooms the exact reply i was looking for, thanks my guy keep doing what your doing we appreciate you for it
Use a high temp flow control clamp, that way you can pc the whole setup.
Link to purchase and see what you are referring to ?
The red stop caps melt got them from your link made 3 jars spray loc in the pc
When you finish your jar, what have you found to be the most efficient way to re-sterilize the stopcock for next use?
These are one time use, if you want to spend the $ on stainless ones you can just chuck them in the PC when you sterilize your grains
Thanks for the quick reply. Great content. Keep up the good work.
Hi. I really appreciate this video, it is very useful information. Wondering if you can use a magnetic stir bar and plate or if the silicone hose will interfere with the stir bar? Thanks
Sorry I just noticed that you have already answered this question. Thanks again for your great videos.
lol any time! Glad you enjoy my content!
Why do we need an injection port? Can’t we use the stopcock to Inject instead of pulling?
I use the injection port if I have a known clean LC I want to expand.....or if I just want to draw one syringe to agar test. Once you use the stopcock it's no longer sterile. Me, I will empty the LC jar into syringes in one sitting.
Found everything but the pp5 lid, great video, I'm going to try this out . Thanks. Amazon does have brass and stainless steel stop cocks. I'll use the plastic ones I think though
You should be able to find those ball brand lids at Walmart
amazing thank you x
You’re welcome 😊
Guys, I can't find the (PP5 Dual M/F Luer Lock Caps) on Amazon to be able to put them in the pressure cooker
Where can I get the injection port and what type of port do I need? Can I use the high RTV gasket maker?
u can also do it with a needle and stopcock
Yes you can
Sorry for thr seemigly stupid question but is a 3 way stopcock essential? Could i use a 2 way one ?
And after u filled the syringes the stopcock stays on the LQ and can be used multiple times? and can you use the stopcock on another LQ again?
Best practice is these are one time use…for me anyway. That’s why I just empty the jar in one sitting and store the syringes in the fridge until I use them
You could spend the extra $$ and get stainless ones that you can re-sterilize
@@RealSimpleMushrooms they're kind a expensive in Germany but I'll have a look at the stainless steel ones. Thanks for the reply!
How do you keep the small amount of liquid exposed to air from getting contaminated?
By using good sterile tek and working in front of my hood or in a SAB
Thanks so much. Nice work! If you see this…what heat sealer are you using & do you like it? I’ve had two now, and neither one seals the whole way thru all layers of my bags!!
It works well but it’s quite pricey
@@RealSimpleMushroomswhat brand and model?
so after you pressure cook the Liquid Culture Nutrient, you'd wait for it too cool off and then inject the initial LC through that injection port ?
Exactly!
@@RealSimpleMushrooms Awesome! Will that filter patch suffice for air exchange? A friend of mine told me to never fully seal jars because its probable it can burst in the PC. I'm wondering if the filter patch mitigates that risk
I’ve never had an issue and I’ve PC’d hundreds of them
Is the stopcock necessary? What if you just took the red luer lock off and hooked the syringe straight to it without the stopcock? Also, how is the stopcock cleaned and used again for next jar of LC? Is this a one time use? Since you cant autoclave it, how do they get sterilized?
It’s not necessary, I like to use them in case I don’t empty the entire jar into syringes in 1 sitting. As far as re-use they are a 1 and done deal.
Great idea. Thanks
You are welcome!
You did not list the injection ports in the materials and you did list syringe filters but did not go over anything about them in the video but had the jar with them on one. So exactly why did I purchase them if I'm not using them and you didn't show what they were for?
So I use the injection ports for 2 reasons.
1. in case I want to inoculate the LC jar with a current syringe I know it viable and clean
2. Sometimes I will pull a quick sample through it to agar test it for contam.
Thank you- very helpful.
Glad it was helpful, and thank you for the kind words.
Hi I’ve been using these lids a couple times now and they’re the dream. Just wondering if it would still be sterile to refeed through liquid culture through the luer lock with PC liquid culture solution syringes? Or have you already tried this?
Very interesting, does that stopcock leak over time? That'd be my only concern because if it doesn't stay air tight at all times gravity will pull the liquid down and draw in dirty air
So for me I will just empty the entire jar into syringes in one sitting, but I also sell cultures. I'll store them in the fridge to slow the growth but they last upwards of a year in the syringe as well
how do you stir it? will stir bar still work with tube
?
Yes the stir bar work with the tube, you may just have to offset the jar on your stir plate a bit
thanks for the walk through! 🎉
No problem!!
Where did you purchase your flow hood?
From Bonsai Filtration
Instead of home made jars, you can use dextrose water 5% serum, medical grade sterile, with self healing injection port, and use serum set for injecting and aspirating, you could buy from a pharmacy
There's a variety of ways to accomplish this, the point of this video is to use readily accessible items anyone can get their hands on.
You're talking about iv saline bags. Yes?
I can see that being effective and easy. I would prefer to leave that much medical waste for medical purposes, though, if possible.
There's also the cost and the volume. I'm not going to need THAT much culture for any one strain, not for a LOOOONG time, anyway. I'd also have to figure out storage. Combine that with the cost per bag (that I've found so far, anyway) and unless I have a family member with "sticky fingers" working in a hospital setting (and I was willing to do that), that could get pretty costly kinda quick.
I'll try these reusable forms. I'll keep in mind the bags as a backup, though. I can actually see them being very organized and efficient in a larger, professional setting even. For a home or small-scale mycology enthusiast, though, I think these will tend to be the better option overall. I also will be trying to minimize how much landfill I produce per "grow" where and when I can.
All that said, if you have sources for smaller bags or for what you see as more cost effective, please let me know? I'd appreciate it!
Good thoughts. Thanks!
Agapé 303
No, I would just take up some sterile water in a syringe, then use the needle to biopsy the mycelium, (push the needle into the agar which should pick up enough mycelium) then just use the sterile water to push the myc into the jar.....does that make sense?@@KOKO-uu7yd
@@KOKO-uu7yd fyi, 5%dextrose water bags, not salin ones, they come in a verity volumes, 300ml up to 1000ml, these sugar bags are relatively cheep, sterile, equipped with self healing injection port, easy to buy from a drugstore , mostly come with sterile serum set which could coupled with syringes and cross connectors for easy usage, could be stored in cabinets for long periods of time, could save time and energy compared to home made sugar jars, with much much less risk of failure/contams.
On the other hand, you may get satisfied if you make your own liquid jar while doing mycology as a hobby at home.
@@RealSimpleMushrooms
It does, i think, for my other posted question? I'm commenting so much im getting confusing, i think! 😂
So yeah, I think you're talking about using the cockstop to inoculate? If i understand correctly, something already liquid should be fine. If I'm working with something like agar or spore prints, I'll still need to have a "carrier" of sterile solution. Other than that, I should keep itnsimple and not over think. I got thst all about right?😂
Also, THANKS! You've taken some considerable time and responded quickly, to boot! Mighty kind😊😊😊😊😊
Why would you need the injection port if you have the tubing
Sometimes I will expand a current syringe, and sometimes I’ll use it to pull a test syringe to put on agar
What would you be injecting into it
Brotha good stuff !
Appreciate it
What about that tiny amount of lc that remains above the 3 way valve ?
Me personally when my LC is ready I will empty the entire jar into syringes in one session. I really only like to access the jar once.
I bought everything in the description and did everything according to this video… Everything went great, except for the little red cap with the Luer lock melted and had aluminum foil covering as well, so what did I do wrong? 😂
No, you didn’t do anything wrong. Those Lids seem to be a little hit or miss. I’ve had some survive and I’ve had some melt, I’m looking for a better solution now.
@@RealSimpleMushrooms there are nylon and PP ones on McMaster. Did you have problems with contamination after the plugs melted? Or should I be good to just swap the caps out while I wait for new ones to come?
First, this was a great video.. THANK YOU !
Second, I just did the same and had the same issue with the red cap.
I went back to Amazon and read the reviews, and older reviews didn't mention an issue with the caps but more recent ones did.
I had just ordered new caps from a lab supply company and will reprocessed the jars when they are delivered.
Not the end of the world , just annoying.
very neat, But aren't you risking contamination more by having to take off that red cap and putting the valve on? And then having to take off the valve cap and putting the syringe on and off ? I love the tubes though. If there was a way to combine an injection port with the tubes (not seperate on the same lid, but together as a single line) I think that would be close to ideal. Have you ever tried that? I aslo dont think you really need high temp RTV as that is usually for the exhaust ares of engines. Regular silicone should be able to handle 250°F easy. Have you tried it and had failures? LOve you videos clear consice simple, very nice. Thanks.
Also any reason to use a 3-way valve when 2-way are cheaper and just as easy to get. And why even use the valve if you cannot sterilize it? Use it once then trash it? I am just trying to understand as I am currently building up my LC cultures and plan to have about 20 jars, and will use at least a full jar a week. for inoculating grain jars
You're correct, any changes in open air you risk contam. Just be sure to do that in an SAB or in front of a flow hood. There are stainless stopcocks out there but they are quite pricey at over 100 buck or more each, but those would be ideal as you could then just PC the entire setup......AND as it turns out those red PP5 caps melt anyway. I made some updates to the description with more information and links.
fyi: Theres many reports on the Dual M/F Luer Lock Caps that say they are not autoclaveable. is this url the same product as shown in the video?
So, I’ve had some survive and some not….
Just wow.
What is the correct size drill bit for this? I ordered the one from the list above and it's a huge drillbit that is much bigger than yours.. just letting everyone know and wondering which is the exact size to order? Thank You
The bit I use is a step bit, each step is a different size hole
I made a very similar one, but I didn't use a 3-way tap. Is it really necessary? Any reason not to connect the syringe directly to the female?
I only used the 3 way because they were readily available on Amazon. The only reason I wouldn’t connect it right to the female on the bulkhead is storage if you don’t pull all your LC into syringes in one sitting. The stopcock gives you the ability to close it up and store it without worrying about contamination
@@RealSimpleMushrooms once closed up and stored to prevent contamination, will a new stopcock need to be installed if you wanted to use the culture on more than one occasion?
Question for you (I'm rewatching now) - you have an injection port, but could one inoculate with lc or spore syringe via the stopcock? Perhaps a flush of sterile H²O would be needed to get everything down the tube and into the medium... 🤔🤷♀️
I'm not sure of this would ve an advantage or not, or even if it would work? Your thoughts?
Thanks again!
Absolutely you can, I put the port there just to have both options.
you would certainly need some sterile water in the syringe as a transport mechanism.
@@RealSimpleMushrooms I wasn't sure if a couple drops would be enough... like with LC. But, I can always try 😊
Thanks again!
as long a some mycelium makes it in the broth you should be good. It doesn't take much.
Off topic if you usehole saw bit drill your hole in reverse as if removing a screw won't break
Step bits still work far better
This is awesome matey!! any reason you chose 3 way stopcocks over 2 way???
They were the most readily available on Amazon tbh
@@RealSimpleMushrooms yeah I’ve analysed your methodology here and it’s really well thought through. With the 6mm ID on the silicone tubing it’s evening amazingly well suited for SSLC, take a peep if you haven’t already come accross it ;)
Those pp5 lids are not pressure cook safe, I just built this exact setup 25 minutes in the pressure cooker deformed and melted the lid. Gotta buy another pack now😢 other than that I’m excited to try out once I get some more
The first pack I bought survived, after that not so much :(
So the red caps are in fact NOT safe in a pressure cooker. Have you tried the ones from McMaster?
I've had some survive and some not, so yea a little bummed about those. I will look into the McMaster ones, thanks for that!
@@RealSimpleMushrooms mine were so melted it was a major pain to get them off and replace them. Hoping I dont get contam as I replaced them while on sterilized jars
Where can I find these McMaster caps 🧢?
Do you use magnetic stir bars and does it get in the way?
Yes I use stir bars, the hose usually has a small curve to it and you can move the jar slightly when stirring to avoid hitting the pickup tube. They work just fine
@@RealSimpleMushrooms amazing man thanks for the tip. Excellent video and thank you for your prompt response. I am in the middle of expanding my LCs and definitely ordering the items from your comprehensive list thank you for taking the time to do that I really appreciate it.
Hello, how do you sterilize everything?
So after I build them I’ll run them through my dishwasher on the sanitize setting then I will put them in a mushroom grain bag and PC them for 60 min at 15psi. I will leave them in the bag until I’m ready to use them.
Thanks
Sure thing!
2:42 polypropylene lids for pressure cooker safe.
sorry for the dumb question: Where is the list of items to buy? Can you reply it to me, rather than directing how I find it?
All the links are in the video description
Soooo Sorry, I've had a stroke, and I'm still stroke stoopid. :-(
Thank you. I see it, should have looked there and I thank you for shared knowledge. Stroke cause me to experience the near death experience, but also to hallucinate for about 4.5 years. I discovered mrooms in my quest to answer, 'what the hell did I see?' Fortunately, mrooms did not reproduce the quality of graphics that I saw. DMT did not produce the quality of graphics I saw. Nor did acid. Salvia Divinorum came closest, and scared the daylights out of me multiple times. However, SD nearly duplicated the quality of the graphics of my near death experience, but I saw unrealistic devices and mechanisms. There was none of that in what I identify as penducular hallusinosis. I did lay prostate while a small man put his hand inside of my head. He also pulled the nerves in my head such that my eye was outturned after the hallucination 😲.
if you want more of these kinds of narratives but with far more detail including being kidnapped and charged with very strange crimes, you might enjoy, I mean appreciate 'The Mysterious Red Light Camera Murder' I'm looking for a publisher, and prissy literary agents are objecting to the use of curse words and low-life expressions such as 'Cops were trying to inventory my dingle berries.'
By far, mrooms were the most prosocial of all the things I mentioned.
@@RealSimpleMushrooms
now that weve covered the basic stuff, how about we get more in depth and talk about recipes for cultures, good substrates for mushrooms and how different species have different needs.
I've heard you can just use honey and water as a liquid culture recipe.
Honey, dextrose, Karo Corn Syrup....lots of stuff. I'll be doing a video on the recipe that I use very soon!
Your information is amazing! Thanks so much for your information especially where to get the items on Amazon.
My question is this: if you don't want to use up all of the liquid culture, can you put a cap on top of the stopcock, after using 70% isopropyl alcohol to sterilize it? It seemss that even if you use a new, sterile stopcock, wouldn't it still have possible contamination when you remove the previous stopcock? Thanks.
PS) I think you should patent it! I've seen the Myco lid using a metal transferring needle instead of using your silicone tubing, and your lids are superior!
As long as your sterile tek is good you should have no problem using the stockcock more than once. What i generally like to do is have a small jar with 70% iso in it and I'll just drop the caps in there while I'm filling, then wipe them down and put them back in when I'm done. It's been working great for me.
My culture is so thick, it’s been refrigerating while using.
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