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So, because I have a flow hood I actually don’t use the sleeve and keep my plates in front of the hood until they are cool and all the condensation has gone away. I don’t usually use the boiling water trick anymore because I have a hood. When I was working in an SAB once I was done pouring my plates I would put the sleeves back on them and then remove the SAB and put the mason jars of hot water on them and leave them until they cool completely

I leave my hood running, if not I would just cover them in the sleeve and keep them in there. They'll be fine.

that's normal, once the mycelium starts to feed on the nutrients in the jar and grow you should notice that cloudiness subside, the jars here with both inoculated the same day.

@ awesome, I’ve noticed some LCs if they’re not stirred often enough they start to colonize and layer of mycelium on the surface of the LC, is this ok or is this actual contamination?

Thanks for watching!

I'm glad you found the video helpful!

Glad you found the tip helpful!

Thanks for the support! Glad you're finding the videos helpful.

​@RealSimpleMushrooms I've been using grafting tape for a while now, since it has pretty similar properties to parafilm, at least the ones we need. (I come from a fruit growing background). Your videos are very informative, devoid of speculation and completely in line with the theory, maximally simplified for a wider audience, and at the same time entertaining, which makes the information easy to understand and adopt. All in all, just as it should be. I wish you, and us, good health and new videos in the New Year 👍🏻

Haha appreciate the support!

I have not, only Lions Mane tbh for gourmets

It happens. I'd say for every 50 plates I pour I get 5 contaminated ones

All part of a plan, I didn’t post for a very long time and I’ve been working on the SEO on the channel so I’m posting 10 videos to give the algorithm a bunch of audience data so it can recommend my videos more and to the right people. It’s been working so far. Every video does better the the prior one. Once I hit that 10 video mark I’m going to get on a schedule of either weekly or bi-weekly with maybe some live stuff sprinkled in

Appreciate the support! Sounds like a good plan for keeping those hands warm.

North Spore, Renegade Mushrooms, and FreshCap Mushrooms are good channels to learn beginner techniques. Lab work is a little advanced.

@@TheUnhousedWanderer I’ve been using the box kits from north spore and stated trying fruiting bags. It’s good to know I’m in the right direction. Yes the lab stuff is somewhat over my head at times, but the more I learn the more my deeper understanding of how it all works increases. Thanks for your advice.

There are a lot of good ones out there but I gotta say Philly Golden Teacher is awesome and all of his teachings are solid. Once you get more advanced (and he has some great beginner content too) is Edward Grand. He’s got a TON of content to sift through

@ thank you 🙏. I look forward to when I can move into the more advanced areas. Great content.

If you have a gas exchange hole or are using a lab media bottle you can screw them in tight, if you just use the metal lid upside down you need to crack that one loose a bit. And yes I have them slightly submerged…maybe an inch or so. Glad you’re enjoying the content. My follow up to this video is scheduled to go live at 3pm est today

Glad it was helpful! Cheers!

It's coming very soon, I'll be filming it today actually :)

@@RealSimpleMushrooms Let him cook!

A “true” casing layer is a layer of 50/50 peat moss/vermiculite. Peat can hold 20x its weight in water and the mixture is non nutritious. It helps with humidity and moisture retention

Appreciate the support!

Happy to help!

Thanks! I'm glad you're finding the videos helpful!

Lol I was only skeptical when I first heard about it but I tried it out and it was great! I still use it all the time :)

Thank you so much for the support and for your generous tip-it truly means a lot! I’m thrilled to hear you’re enjoying the content and finding it helpful, especially with agar work. Your kind words really keep me motivated to keep creating and teaching! You’re absolutely right-mycelium is incredibly resilient. On water agar, it will often stretch out quickly in search of nutrients. This makes it an excellent tool for outrunning bacteria, as the mycelium usually gets a head start where you can snag a clean sample. I’m glad to hear you’re diving into these techniques-it’s a game-changer once you get the hang of it. As for pouring agar, we’ve all been there! Getting the timing right to avoid condensation can be tricky at first. The good news is, I’ll be filming a video soon covering proper pouring techniques and sharing some tips to combat surface water and condensation. Stay tuned-it’ll be up shortly! Thanks again for your amazing support and for sharing your journey-it’s awesome to hear about your progress. If you have any questions or want to share updates, I’d love to hear from you! 😊

@RealSimpleMushrooms that is fantastic! Both, the upcoming content and your being open for hearing updates and more feedback. You are welcome. When I find value in something that someone shares I try to grateful and mindful to award their efforts. You upped my agar game and inspired me to do my 1st pour 🫗 Well, actually I found you as I was pouring. Lol

@RealSimpleMushrooms and..... your thumbnails are rocking. The colored agar plates pop. And your vids look clean. Audio is good. Topic? Spot on with where i am in this agar game.

Happy New Year! Appreciate the support!

Not 100% of the time but it is an indicator

not sure I follow lol

That’s why I put timestamps in the description, so you can skip to what you want to see

Lol! Don't hate the player, Hate the game!

Tik Tok is the platform for people suffering from ADHD lol

I really appreciate the support and feedback! I try to bring value to the channel with every video, and I'm glad you appreciate it.

I'll have to try that, thanks!

You need to have the blade hot and turn it sideways to carve a gap with the broad side of the blade. It needs to be a clear gap all the way to the bare Petri dish.

You can really use either. The meat will speed up the growth needed to achieve the hopefully clean transfer. But personally I prefer to make that transfer to water agar..then transfer one more time after that back to MEA

@@RealSimpleMushrooms thanks for the response! i put it back to water agar this morning , but i find the growth incredibly slow and very fine strands ... I will try both these methods on water next .... i was on T6 and it was making me crazy with the aseptic technique .....i was considering antibiotic agar , but i would rather avoid if possible

You only need one of those fine hairs ;)

When I put something to water agar usually it will go rhizo (but be very faint) and as soon as it shoots off from the transfer I’ll grab a piece and toss it back onto MEA

@@RealSimpleMushrooms Thanks for the insight !!

Thanks, I appreciate the support! I hope to make it to 10k!

Good luck!!

¡Feliz año nuevo para ti también, amiga mía! Me alegro de que los estés disfrutando. Gracias.

Happy New Year! Let's get growing!

Glad you liked it! Let me know if you have any questions.

Glad it was helpful!

Thanks man, I appreciate the kind works! Thanks for joining along!

Let me know how they work out for you!

@@RealSimpleMushrooms I tried a no pour version on glass plates. Your recipe for lme. The sterilization bags sorta broke down. So I iso'ed 70 the area as I removed the plates and wrapped them to seal them and ziplocked them. Maybe tent the bags in foil next time. In a week I'll see if they are still clean and test some lc samples I have saved. The stir plate and Corning Pyrex are useful for coffee too lol. Already had a nice pressure canner that made it easy. Lc to agar testing and spore to agar are my next in line.

lol this is satire but if it’s a shoebox tub, I just toss it

​@@RealSimpleMushroomsshoeboxes costs 2 dollars aprox. so... directly to the trash hehe

Not if you buy a 10 pack ;)

:) thanks for tuning in!

HAHA, thanks man. you know as well as I do how badly people overcomplicate things.

That's a great point. I do check my jars, I had one burst in my autoclave once lol

I'll have to try it cold sometime.....never even gave that a shot tbh

Thanks for the kind words! Glad to have you on board. Twisted Tree makes great products, you'll be very happy with them! Drippy corn tek works great because mycelium loves sugar! I've been adding dextrose powder to my grain for a long time! Same concept, just a purer form of corn sugar :)

Short answer, cloning you're looking for specific traits (large fruits, large clusters) to hopefully continue to grow that same phenotype (its takes a few selections and then going back to spore to stabilize it, that's a much deeper conversation) Spores are how you pheno hunt. I will swab a plate with spores and as long as they are clean I will just take that whole plate right to grain and grow it out and start hunting. I will also try to isolate a monokayron culture for potenial breeding down the road (again another deep topic)

@@RealSimpleMushrooms Thank you very much for taking the time to answer me, you really answered the question I needed.

Anytime :)

@@RealSimpleMushrooms I have another question if you don't mind, I was growing certain genetics with peat, worm castings and vermicurite. (with great resoults, yields and flushes) Some easy genetics.(like creepers or golden t) Now I wanted to try some exotic ones like yeti and I have noticed that the fruits come out too small. Could it be that some species like different types of substrate? I have had bad experiences with coconut and vermicurite so I decided to make my own substrate.

That recipe works so good!

You're welcome! I hope you find them useful.