How to Spot Contamination in your Agar, Grains & Liquid Culture
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- Опубликовано: 19 мар 2024
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🍄 Contamination is every mushroom cultivator's worst enemy! Sometimes, it can be challenging to determine which of your mycelial growth is healthy and which is contaminated. Jasper goes through the common contaminations you will find in your agar, grains, and liquid culture, as well as the 7 Vectors of Contamination that will help you avoid contaminated mycelium in the first place!
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When my husband started growing mushrooms he lost a lot of them to trichoderma. I couldn't understand how things got so bad without him noticing and then we realized it's because he's red-green colorblind and could not tell it was green until it had completely taken over. Now I go through and check things for him 😅
but how do you fight tricho ? normally I throw it away😅
@heidik.9005
as someone who has grown (and is growing) just about every type of mold feasible, i have to say, thats some pretty gnarly mold in those dishes, especially on that gourmet TAT strain. Lordamercy, that bad boy is a biohazard.
Welcome to the jungles of Guatemala haha
I agree it’s crazy he even suggested doing a transfer to an audience of mainly new growers.
Great tips. Keep up the amazing work!
Thank you Mr Mono🐵
Thank you for the awesome content! I always struggle to ID contam in my LCs.
Thanks for watching! Altho sometimes you can see contam in LC I always test mine before using it
Great info!
Glad it was helpful!
Great vid...thanks for all you guys contribute. Would love a vid just looking at a bunch of agar plates and describing what you are seeing. This would include contam, but also various kinds of growth patterns. Even after looking at hundreds of plates, when I see mycelium taking on multiple phenotypes, I'm always curious/nervous about what is going on and if I might be missing something. We are at +7000 feet so things are pretty clean, but getting a good sense of what we are looking at is invaluable. Mush Appreciation!
That's where the practice comes in! It'll become like a second nature one day. Thanks for the sweet words!
Contams can also be embedded in the mycelium.
Or endospores, it's all an intricate web
Thank you for this video.
I have a question for you concerning contamination.
I spawned a green jar with PE.
The inoculation was coming along very well, with most of the spawn jar colonized, but recently I noticed colors in it that I have not seen in other jars and I'm wondering what they mean and if it is contamination?
The green was not normal green of trichoderma but was a bright neon green-blue.
Also, there was a bright neon pink in it as well.
I was happy to attribute the blue-green as bruising, but I am bewildered by the pink...
Pink is generally other fungi meaning your spawn is bunk unfortunately
How do I come out there with you guys lol
when i first started growing mushrooms, the place where i was at had a problem with that orange bread mold stuff. it's hard to catch because it grows like mycelium, white n fluffy, until it matures and turns into an orange dusty spore mess that gets everywhere. has a putrid dead-fruit smell. When i first started growing my subs were way too moist, for sure. lol i adjusted quickly and ended up growing over a dozen species. I love mushroom so mush. :*)
Neurospora crassa is the worst.....
This question isn't so related to this video, but I wanted to ask this in the more recent content hoping for an answer. Can anyone tell me of where, in the US, I can send my medicinal mushroom extracts to have them tested for desirable bioactive constituents?
Mmm it's difficult testing needs to improve
What smells minty as far as contamination?
Mint? Jokes aside I've never had this
I was confused as well. Notice the funny smell started looking closer and found a nearly white light grey in a cake that was just put into fruiting. I cut the spot (size of a half dollar) out and just as a test I sprayed it with alcohol it immediately turned blueish green. The cake went on to pin and the smell came back. I couldn’t find it this time and all pins aborted. I disposed of that cake.
How can I solve with chemical treatment
So I'm a new grower..My first grow actually..I inoculated 2 grow bags..I noticed about 4 weeks in green spots of trichoderma in the mycelium colony. So I was thinking.. And it occurred to me..😊 CDS.. It's a water purifier.. It's a water soluble gas.. And kills fungi bacteria viruses on contact.. But when it's not contained, it does what gas does and evaporates, leaving sterilized water.. So I used a watered down solution very low ppm.. I took a funnel and put the bottom of the funnel right on top of the green spots..Dropped litterly 1 drop at a time..And it killed the trichoderma immediately.. And in seconds evaporated leaving the mycelium un harmed..7 days later still no signs of the trichoderma.. So this might be them chemical solution you were asking about..
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Why would you even suggest doing a transfer from that TAT disc Contaminated with black mold that is wild. Everything you said was pretty relevant until I heard that then that’s all I could think of for the rest of the video that is a terrible idea. Not only are you running the risk of letting that contamination spread like wildfire into your work area and other grows but that stuff is really horrible for you and can even kill you. There’s some things that you can save and there’s other things that you just discard and that’s one of them.
Honestly I toss any contamination because I have back ups of everything. But I've also been in scenarios where I only had one plate, contaminated with black mold and no back ups order spores and made clean transfers from them so yea I think it's valid information to share
You almost look like your flashing gang signals there bro. I was half expecting you to break into rap.
👌🖖🤘🤙
So like if you get tendonitis, do you have to go to a speech therapist ?@@Fungiacademy
Bravo,very nice video,loads of knowlange in it. TY
Thanks for the kind words Janhus!