Currently im studying at a medical college in Republic of Korea, and honestly you are a true life savior Thx a lot :) Ps. After watching this lecture, eating a chocolate chip cookies :D
I've watched your lecture on Gel Filtration Chromatography as well as this one and you explain the processes SO much better than my PhD University lecturer does so thank you!
Gracias por esto, que simple y facil de entender lo haces. Veo tantos comments de gente agradecida. De verdad eres un heroe, mis respetos y muchas bendiciones para ti. Saludos desde Puerto Rico.
This was the height of perfection in teaching.. Thank you so much Sir... for making such difficult topics easy to understand.. love all your videos ..:)
WOOW I have a lab today about this and I didn't get what was written so searched on RUclips. You really explained everything so well. Thank you soso much :)))) Great video really.
Ak lectures is my favorite channel. by the inspiration of this channel i started my channel covering all topics regarding pharmaceutical analysis & biochemistry. i did video on ion exchange chromatography
Thank you so much for making these videos, they are so helpful and you are such an amazing teacher. Im able to understand these concepts clearly and concisely. I appreciate you too much, thank you!
While mass of any protein is not 0, suggesting that gravitational force pulls it down the column is a bit misleading to say the least. Also when you have electrostatic attractions between opposite charges in the column it is silly to suggest that anything that is electricly attracted to the stationary phase in the column will ever move down the column, unless you balance the charge between the stationary phase and that particular protein or whatever species you're trying to purify.
Outstanding. These concepts aren't always that complicated, but they are often explained so obtusely that it hinders comprehension. I think it is a skill to be able to communicate these things without such obstructions and you guys do this consistently.
oh man! you are absolutely beyond perfect! every time I need to have a better understanding of a subject and I search it on youtube your amazing videos are on the top of the results and they are completely enough.
hi, thanks for the clear explanations. I have a doubt- How do we know when it's time to collect the respective eluate 1, 2 and 3? as in real life, the solution would be all same in colours.
What if our protein of interest is neutral. How do we adjust the PH to give our Protein a charge (example by lowering the PH against the PI to make our Protein positive (protonation) or increasing the PH by 1 unit above its Isoelectric Point to make it negative -deprotonation). As against what you said, we c
I like what you are saying and the tempo is good in your videos (i have only watched a few about chromatography)! On your previous videos about chromatography, it seems to me that you are shouting when talking. But in this video you are more calm - stay like that! :) cheers
I forgot everything of chemistry after graduation. At first, i tried to get glimpse of this because there were so many words.. However, your explanation was so clear and i already watched your other lectures. I'm really appreciated with your explanation.
So I have tripeptides that are +2,0, and -1 in charge moving down a positive column. I know that the tripeptide with the +2 charge will elude first but will charge 0 or -1 come after??? URGENT!!!
sir dont the net negatively charged proteins get repelled immediately when they come in contact with negatively packed column of gel beads and stay at top itself little bit confused (like charges repel)and the net positive charge should come to the bottom getting attracted (unlike charge attract)and here gravity also fvrs would be thankful if u could reply anyway good video sir
THIS WAS PERFECT!!! I even clapped at the end! GOOD JOB!
+Sheri Berry lol! thank you Sheri! :)
Currently im studying at a medical college in Republic of Korea, and honestly you are a true life savior Thx a lot :)
Ps. After watching this lecture, eating a chocolate chip cookies :D
Thanks you!! Why can't teachers explain that well!?! Life saver!
qdz1005 Thank you!!! :)
I've watched your lecture on Gel Filtration Chromatography as well as this one and you explain the processes SO much better than my PhD University lecturer does so thank you!
Gracias por esto, que simple y facil de entender lo haces. Veo tantos comments de gente agradecida. De verdad eres un heroe, mis respetos y muchas bendiciones para ti. Saludos desde Puerto Rico.
This was such a perfect explanation! I loved the analogy too - I wish you were my BioChem professor!
You are a really good speaker and teacher. I understood everything 100%. Subbed. Thanks!!
Best biology teacher in the world . No one can better than AK lecture
This was the height of perfection in teaching.. Thank you so much Sir... for making such difficult topics easy to understand.. love all your videos ..:)
You are amazing and your teaching is so perfect.. Thank you for this
the way you explain things is simply awesome
WOOW I have a lab today about this and I didn't get what was written so searched on RUclips. You really explained everything so well. Thank you soso much :)))) Great video really.
NOT ALL HEROES WEAR CAPES!
You're saving lives here.
Respect.
Thank You SOOOOO much! I have a Biochem exam on Monday and your videos are extremely helpful and informative!
Ak lectures is my favorite channel. by the inspiration of this channel i started my channel covering all topics regarding pharmaceutical analysis & biochemistry. i did video on ion exchange chromatography
OMG this was so good I didn't get the lecture slides but after watching your video, everything is SO clear!!!!!!!!!!!!
Thank you so much for making these videos, they are so helpful and you are such an amazing teacher. Im able to understand these concepts clearly and concisely. I appreciate you too much, thank you!
this is called PERFECTION! saves a lot of time by watching this
Very good job ,I really like this site . Analysis made easy henceforth.
thanks,man! prepared for my exam now due to ur explaination 😉👍
Explained perfectly, thank you!
awesome teaching style!! wont forget this concept ever!!
Chirag Mohan Sharma thank you! glad to hear the concept sunk in
While mass of any protein is not 0, suggesting that gravitational force pulls it down the column is a bit misleading to say the least. Also when you have electrostatic attractions between opposite charges in the column it is silly to suggest that anything that is electricly attracted to the stationary phase in the column will ever move down the column, unless you balance the charge between the stationary phase and that particular protein or whatever species you're trying to purify.
Your videos are so helpful. Have been watching these for years and I'm almost through university. Thank you!!
Your explanation is great
I love it 😍
Omg, this explanation was awesome! Thanks man, very helpful.
Perfect explanation!
Outstanding. These concepts aren't always that complicated, but they are often explained so obtusely that it hinders comprehension. I think it is a skill to be able to communicate these things without such obstructions and you guys do this consistently.
Chocolate chip cookie analogy was brilliant. Just finished watching 34 videos, and you got 34 likes :) More to come.
haha thanks! :)
I understand everything now, thank you so much !!!
Awesome video...why ads not coming to this channel...i hv been watching this since 6 months..
You have videos for everything I need for this course I'm having!
Wow, simply outstanding! You present this material so clearly and so well! Thank you so much!
Thanks Chris! glad my style of teaching is to your liking :)
Medical university level, good job sir!
OMG!!! Outstanding, you Sir!
oh man! you are absolutely beyond perfect! every time I need to have a better understanding of a subject and I search it on youtube your amazing videos are on the top of the results and they are completely enough.
hi, thanks for the clear explanations.
I have a doubt- How do we know when it's time to collect the respective eluate 1, 2 and 3?
as in real life, the solution would be all same in colours.
Thank you so much!
excellent!!!
What if our protein of interest is neutral. How do we adjust the PH to give our Protein a charge (example by lowering the PH against the PI to make our Protein positive (protonation) or increasing the PH by 1 unit above its Isoelectric Point to make it negative -deprotonation). As against what you said, we c
I would never elute from that column containing chocolate chip cookies. No way! :-)
You open my eyes ! Thank you for this hard works indeed! Love this !
Your videos are so helpful . tnx
Wouldnt this require prior knowledge of how charged your protein is relative to the mixture?
You are the best 😭😭😭
thank u. it was fantastic.
I am the kid that will stop for the chocolate chip cookies! Well presented btw!
me too! :)
THANK U SO SO MUCH!!!!!!!!
I like what you are saying and the tempo is good in your videos (i have only watched a few about chromatography)!
On your previous videos about chromatography, it seems to me that you are shouting when talking. But in this video you are more calm - stay like that! :) cheers
awesome tchr
I have a question. In diagram E after we pour the salt solution, won't it collect in the beaker with the protein?
now i'm hungry...dammit
:)
Thanks much, u really great , simple teaching and clear voice , i have only one request , if u can zoom alittile on the board
such a useful lecture,, thanks
Really helpful, thank you!!! :)
Understood ..thank you
I forgot everything of chemistry after graduation. At first, i tried to get glimpse of this because there were so many words.. However, your explanation was so clear and i already watched your other lectures. I'm really appreciated with your explanation.
OMG!!! Why my teacher is not like that!!
why don't the proteins with positive and negative charge show coulombic interaction??
ما شاء الله , دقة الفيديو عالية ,, أبدعت بالشرح ,, شكرًا
Chacklate Chip Cookies
"in the following diagram"
Perfect💯
How would you tell where the boundaries are between the proteins as you collect them in tubes?
Would a Zwitterion with a neutral charge elute before charged proteins of either kind?
Amazing explanation!!!!!! It helped me so much!!!!!
Wow, that was explained very well. Thank you, if I could give you a chocolate chip cookie I would definately do it :D
thankyou sir
Best ever teaching method👏 👏 Thanks for making this subject interesting..
THANK YOU SO MUCH
Part with chocolate cookies - super!!
Thank you for very very nice lecture!!
Excellent video that I have seen about ion exchange chromatography, thanks a lot.❤
I hardly ever comment
but holy shit
I learned something that my professor couldn't do all semester long.
Thaaaank you very much. really good presentation.
So I have tripeptides that are +2,0, and -1 in charge moving down a positive column. I know that the tripeptide with the +2 charge will elude first but will charge 0 or -1 come after??? URGENT!!!
What about the case of negatively charged proteins and neutral ones? Will the negatively charged reach the bottom first?
BEST BEST!
I was searching this answer for the Whole Day, yes whole day.
And here it was
Thank you
Thank you kind sir, excellent review for tomorrows lab!
Thank you sit
you are great 😍😍😍😍😍
Can we pour the salt solution from the beginning ?
U did areally good job.... Many thanks
You remind me of leonardo dicaprio in wolf of wall street - your sound and mannerisms! Anyways - thank you- this is brilliant
perfect جزاك الله كل خير ^_^
Thank you for this! God bless you!
sir dont the net negatively charged proteins get repelled immediately when they come in contact with negatively packed column of gel beads and stay at top itself little bit confused (like charges repel)and the net positive charge should come to the bottom getting attracted (unlike charge attract)and here gravity also fvrs would be thankful if u could reply anyway good video sir
the proteins need to be denaturated for ion exchange chromatography?
Thank you so much for doing this for free.. you are awesome
Great video, and great analogy with the chocolate chip cookie kids! Thanks for this :)
nice teacher.thank you very much for your work. only god will reword you
It is stupid that you are wearing a white shirt with a white background.
how about net -ve and large net -ve charge? which one elute first?
Thanks so much! You made it easy to understand!
that was cruel man, poor allergic kid
What about the chloride ions that you pour down from your salt solution. Should those be collected in a separate test tube?
Can anyone explain on what basis we can select weak or strong cation exchanger for a protein separation
well understood..thank you
I was just thinking about cookies
Thank you very much sir
perfect and easy thank you!
All of the videos that I've seen by AK Lectures are well very done and SO HELPFUL. You do a great job, thanks!!
Thanks Helen!
and you explained shame!#in a zimbo way