Great video! I will be looking for a video of yours with greater detail of what happens to the analytes/proteins when exposed to a pH gradient during ion exchange chromatography. Thanks!
That's pretty clear information but I don't understand the content. This is where I love science but don't always understand it. Then I get frustrated and zone out. It's really not a criticism to the presenter at all. It's knowing myself enough to know I don't understand. With science I know I am much better at the questioning than the answering. I know in science I need very practical everyday examples to understand things.
Every one Read this please. Doubt clear Ion exchangers are either cation exchangers, which exchange positively charged ions (cations), or anion exchangers, which exchange negatively charged ions (anions). There are also amphoteric exchangers that are able to exchange both cations and anions simultaneously. The resins consist of aggregations of big insoluble molecules carrying fixed negative charges, which loosely bind positively charged ions (cations); these readily exchange with cations in the fluid environment to an extent that depends on their affinity for the resin and their concentration. In cation exchange chromatography, the stationary bed has an ionically negative (-) charged surface while the sample ions are of positive (+) charge. This technique is used almost exclusively with ionic or ionizable samples.
Quick biochemistry basics is my favorite channel. by the inspiration of this channel i started my channel covering all topics regarding pharmaceutical analysis & biochemistry. i did video on ion exchange chromatography
thank you for your effort . but l have a notice for you, My friend, Cation Exchanger have a positive charge,while Anion exchanger have a negative charge. thank you again for you .
Not true. The exchanger itself has the charge opposite that of the ion that is to be bound. If you want to bind a cation to the exchanger, it need to be negatively charged.
Nothing simple about this for sure. Can you make a short one explaining for lay people. Our local water plant just switched to this type of water cleaning and I'd like to know if it means they'll use less chlorine now.
Then you need to look for a video explaining ion exchange. Ion exchange Chromatography is a system solely for detecting substances such as proteins. The system of ion exchange (without chromatography in the name) is for filtering. But I don't know anything about water filtering so I can't help you :/
bro you are correct that cation is positive charge but in case of ion exchange if we have cation exchanger. it will have negative charge on it and will attract positive charge and this will be able to exchange the cations and hence it is termed as cation exchanger. here the name and charge are of opposite nature cation exchanger - negative charge anion exchanger - had positive charge on it.
this channel saved me again
Night before exam vibes 😂
😂😂....mine before 2 days 😂😂😂😂😂
Same here
Mine in few hours
For reall 🥲🥲
I'm glad this is in English. South Indians greatly benefit if it is in English as opposed to Hindi
Not everyone is about you guys?
Very trueeeeee❤
Thankyou...in only 3 mins you explained the whole concept!!! Very helpful..!!
Great video! I will be looking for a video of yours with greater detail of what happens to the analytes/proteins when exposed to a pH gradient during ion exchange chromatography. Thanks!
That's pretty clear information but I don't understand the content. This is where I love science but don't always understand it. Then I get frustrated and zone out. It's really not a criticism to the presenter at all. It's knowing myself enough to know I don't understand. With science I know I am much better at the questioning than the answering.
I know in science I need very practical everyday examples to understand things.
Just our things man
Science man, it's hard
Every one Read this please.
Doubt clear
Ion exchangers are either cation exchangers, which exchange positively charged ions (cations), or anion exchangers, which exchange negatively charged ions (anions). There are also amphoteric exchangers that are able to exchange both cations and anions simultaneously.
The resins consist of aggregations of big insoluble molecules carrying fixed negative charges, which loosely bind positively charged ions (cations); these readily exchange with cations in the fluid environment to an extent that depends on their affinity for the resin and their concentration.
In cation exchange chromatography, the stationary bed has an ionically negative (-) charged surface while the sample ions are of positive (+) charge. This technique is used almost exclusively with ionic or ionizable samples.
Life saver for quick revision ❣️✨✨🥺
Quick biochemistry basics is my favorite channel. by the inspiration of this channel i started my channel covering all topics regarding pharmaceutical analysis & biochemistry. i did video on ion exchange chromatography
Thank you for your amazing work ❤️
Watching this one hour before my exam
Thank you for your presentation sir!
Wow . What a quick key to learn. Great
Thank you for this amazing video.
Keep uploading such more videos.....it helps a lot
Shouldn't cation exchangers use positive buffers?
Very nice and concise explanation!!
Amazing video ✨
Very helpful vedios. Thanku you very much
thank you for your effort .
but l have a notice for you,
My friend, Cation Exchanger have a positive charge,while Anion exchanger have a negative charge.
thank you again for you .
Not true. The exchanger itself has the charge opposite that of the ion that is to be bound. If you want to bind a cation to the exchanger, it need to be negatively charged.
Well explained
Hello. Great video this helped me a lot.
Thank you ×100000times
Fantastic explanation. Thank you
description very helpful. thanks so much.
U r doin best work
Somewhere I’m unable to understand
Cation have + charge attract - charge ☺️☺️
Cation is postive or negative charge
A cation has a positive charge, but a cation exchanger has a negative charge, therefore it attracts cations.
@@camilasummers thank you i was having the same doubt too
Today is my exam nd I came here to quickly understand the concept😅
Like it 😊
Great!
Zbardast
Small mistake: cation is positively charged. You guys said it was negatively charged
they meant negative charges are attracted to it
Nothing simple about this for sure. Can you make a short one explaining for lay people. Our local water plant just switched to this type of water cleaning and I'd like to know if it means they'll use less chlorine now.
Then you need to look for a video explaining ion exchange. Ion exchange Chromatography is a system solely for detecting substances such as proteins. The system of ion exchange (without chromatography in the name) is for filtering. But I don't know anything about water filtering so I can't help you :/
hey, what is the role of silica beads in ion exchange chromatography
separation based on size bro
It is a best polar solvent so the separation is easy
what the function of buffer?
Thank you so much❤️❤️❤️🥺
Thank you so much 😊❤
Sir plz make videos on centrifugation technique
Suuper
thanks for the video but the fake accent reminds me of my organic chem teacher .hahah
Thanks
Thank You good man
How to change the ionic strength can anyone explain please?
Change in salt concentration of mobile phase could do it.
please make animation video for this
Thank you 🙏
Cation is positively charged not -vely charged bro.
bro you are correct that cation is positive charge but in case of ion exchange if we have cation exchanger. it will have negative charge on it and will attract positive charge and this will be able to exchange the cations and hence it is termed as cation exchanger.
here the name and charge are of opposite nature
cation exchanger - negative charge
anion exchanger - had positive charge on it.
thank yooooooooooooooooooooou ♥♥♥
Just wow
thank u
Thank you❤
thanks, but no music please.
Perfect
Tomorrow is my exam😂😢😢😢
Today is my exam😢
Thanks you
Spr
thank you!!
I have an exam after 1h😅
thanks.
Thankyou
Thank you
(CATION +VE) (ANION - VE)
Whose here because they took Bachelor's in science in Biology? Cause I am.
Tomorrow is presentation 😅😂
wow
In 1 hour is my exam
Tomorrow is my exam
🙏 ❤
05/09/24 is my exam.(Sem-ll) - thank you sir❤
1:40
I understood nothing oo😂😂
pog
t`pu temge temge temge
An accent which can be produced by eating wadao with champagne.
Why fake an accent bruh!
Ayn
Tomorrow is my exam
3 days ago was your exam
Today
Tomorrow 😅
2 months ago was your exam
Same
Perfect