oh dude thank you. finally a way to understand this. the terminology of a gas chromatograph is used so much with mass spectropy that its difficult to figure out whats what. i had always thought those peaks on the chart were speaks on a spectrum! this has cleared a lot up for me, thanks.
summary of the video: GC is used for the separation of volatile compounds( compounds which are easily vapourized at room temp) Components of GC: 1) SAMPLE is mixed withe a highly volatile compound like Hexane, cyclohexane, etc. • Long coiled column: can either be a packed column(made of glass/stainless steel) or Capillary column (made of fused quartz). this column is placed in a CHAMBER in order to facilitate a uniform temperature. (HIGH TEMP) During analysis, placed at 150-300°C • the SP is packed in the column. it is usually made of silicon grease or wax as it can withstand high temperature • Presence of a SEPTUM to inject sample just before the chamber - the temperature of the injection region is 20-50° higher than chamber. this is to facilitate the rapid vapourization of the molecules. 2) Mp: usually helium / nitrogen (inert/unreactive gases are used) are placed in a cylinder connected to the Column via the Molecular sieve. 3) Molecular sieve is used to filter out unwanted HCs, Water vapour, oxygen etc that may interfere with the sample during experimentation. 4) Detector
Detector - common: FID ( FLAME IONISATION DETECTOR ) which has 3 inlets: • carrier gas from the column • hydrogen • oxygen ignitor ignited the H2 and O2 to produce a flame. when sample molecules reach the flame, they get ionized and electrons are released. across the flame, there are 2 electrodes ( cathode for cations, and anode for anions) the electrons generated are detected by the electrodes in the form of current and it is amplified by the amplifier/Computer.
the computer then generates a graph: X axis- time y axis- current based on known samples and comparing their output with the output of the unknown samples, the retention time/concentration can be deduced
I work for a company in the Chemistry industry that manufactures instrument and that was pretty accurate, good job. Only thing is inlet is at 20-50C higher than the max temperature reached by the GC and there is also an inlet of Makeup Gas for the FID which is usually to compensate the fact that nitrogen or helium coming from the column is usually much lower than the other gases. Other than that, great video
Okay but what about when two samples have similar or even the same retention time? I use LC-MS/MS as a main tool for my PhD work but I was curious about how this other technique works.
You can use a different phase to achieve different RTs. There are some that will never work on GC. For instance, M,P-xylenes are not chromatographically separable. However, using a HRMS will differentiate the identifications.
@@lion-o848we simply don't care whether you understand Hindi or not. There's a translation option underneath the comment if you want the comment in English.
2:45 I don't understand when the temperature of the injection in 20-50 degrees Celcius and the column is 150-300 celcius which means the column is higher, but you said and is given in the picture that the temperature of the injection is higher than the column, does that mean the temperature of the injection is 150+20 degrees celcius? where we add that 20 and 50 ? Please can I get an explanation🥺
A detailed information in a very shorter period of time and was explained in a crystal clear manner. Excellent.
Last sem exams I prepared some of difficult topics from your channel and got a 86/87 marks..
WOW! I am still repeating.
Wow online me itne marks.😜
Thanks a very lot.i studied A to Z clearly from this video.it's amazing how you explain them simply.please make more & more videos.Good Luck
oh dude thank you. finally a way to understand this. the terminology of a gas chromatograph is used so much with mass spectropy that its difficult to figure out whats what. i had always thought those peaks on the chart were speaks on a spectrum! this has cleared a lot up for me, thanks.
summary of the video:
GC is used for the separation of volatile compounds( compounds which are easily vapourized at room temp)
Components of GC:
1) SAMPLE is mixed withe a highly volatile compound like Hexane, cyclohexane, etc.
• Long coiled column: can either be a packed column(made of glass/stainless steel) or Capillary column (made of fused quartz).
this column is placed in a CHAMBER in order to facilitate a uniform temperature. (HIGH TEMP)
During analysis, placed at 150-300°C
• the SP is packed in the column. it is usually made of silicon grease or wax as it can withstand high temperature
• Presence of a SEPTUM to inject sample just before the chamber - the temperature of the injection region is 20-50° higher than chamber. this is to facilitate the rapid vapourization of the molecules.
2) Mp: usually helium / nitrogen (inert/unreactive gases are used) are placed in a cylinder connected to the Column via the Molecular sieve.
3) Molecular sieve is used to filter out unwanted HCs, Water vapour, oxygen etc that may interfere with the sample during experimentation.
4) Detector
Detector - common: FID ( FLAME IONISATION DETECTOR ) which has 3 inlets:
• carrier gas from the column
• hydrogen
• oxygen
ignitor ignited the H2 and O2 to produce a flame.
when sample molecules reach the flame, they get ionized and electrons are released.
across the flame, there are 2 electrodes ( cathode for cations, and anode for anions)
the electrons generated are detected by the electrodes in the form of current and it is amplified by the amplifier/Computer.
the computer then generates a graph:
X axis- time
y axis- current
based on known samples and comparing their output with the output of the unknown samples, the retention time/concentration can be deduced
@@sequeira7330 seems like you're a technician of GLC...
Excellent explaination bruh🎉
Thanks 🙏 to your efforts, we finally understand the different types of chromatography
O my God,,,such an amazing explanation
Love it,,, clear my all doubts and confusion
Respect 🙏 from Africa Zambia better than my lecturer for a year thanks I know love this course❤
Very brief and to the point. Love it. Thank you so much.
Well explained, better than my lecturer tbh thankss
Very well done! You're really helping us beginners! Thank you!
I work for a company in the Chemistry industry that manufactures instrument and that was pretty accurate, good job. Only thing is inlet is at 20-50C higher than the max temperature reached by the GC and there is also an inlet of Makeup Gas for the FID which is usually to compensate the fact that nitrogen or helium coming from the column is usually much lower than the other gases. Other than that, great video
thank u, u are so helpful, more helpful than my lecturer
Stupendous!! It is great effort
You have make it clear as crystal 🔮 mirror
Thank you very useful for my exams
Thank you this was the exact format and presentation I needed
Good explanation sir with animations 🙏🙏
Amazing lecture clear my all ambiguous points about GC
Amazing.....the video clear the concept of chromatography.
Thank you so much ..well explained ..very informative.
I like these videos. They just make my life easier. Thank you so much for the explanation!!!!!!
Excellent Work, Thumbs Up.
Respect from Pakistan 🇵🇰🇵🇰🇵🇰
This video made my day today
Very very very very helpful
Thanks alot for this
this is amazing. so simple and clear. thank you!!!!
thank you for this informational video on behalf of all science students :)
i have a organic chemistry lab tonight and hoping that it goes well. thanks for the video, it's very insightful :)
Well explained briefly. Thanks
thanks dude, very clear and helpful!
thanks for this video you explained
very well
Really good video, made me understand GC!
very well explained understood it greatly
COOLSOME data.It's awesome staff you arranged here.
the tography part of the word is pronounced like photography
hope that helps
very well done video very good keep it brother
Amazing! Very well explained. Thank you very much.
good explanation and it will help me more..thanks
Thanks a lot for understanding easily and clear concept
Thank you exactly what I needed.
Thank uuu sooo much for this wonderful explanation 👏👏👏
Very helpful 👍 plz do classes of HPLC and spectrophotometer
You are absolutely amazing! Thank you So much :))
impressive..beautifully explained
Nice one explanation i always found there
In bad need for applications of gas chromatography 🥺💔
Excellent video. Very clear and informative. Good job mate!
This video totally cleared everything up, thank you so much
Excellent teaching
Brilliant explanation dear sir☺️❤️
Thank you very much!! May I have the references for further reading please?
great video, great explanations, and i love your drawings/ animations!
Good explaination
Well explained
Thank you
Respected mam, kindly make videos on spectroscopic techniques and cover all thier types separately. Thanks
Amazing explanation
Excellent video! Thanks
thank you sir for your good explaination.
So very good.Thank you!
Very useful ☺️👍 thank you
no need teacher !!after this video😀
WELL EXPLANATION EASILY UNDER STAND THANK YOU
That was helpful, thank you for sharing
Thank you for your video, it's clear
This was very helpful. Thank you :)
Thanks you made it simple
I use Shimadzu GC
Thank you for this🙏
Well explaination
Really well explained thank you!
Thank you Sir.❤️🙏🏻
Amazing sirr
Thank you. Very helpful
Thank you 👍
Well done sir
very well explained 2:20
really helpful !
It's really helpful ...thanks 💜
Good video, thanks
As we can say in 6min our separate sample is there??
Super..very useful.. simple
In fid carrier gas was nitrogen/ helium which carries the sample to burning area?
Okay but what about when two samples have similar or even the same retention time? I use LC-MS/MS as a main tool for my PhD work but I was curious about how this other technique works.
You can use a different phase to achieve different RTs. There are some that will never work on GC. For instance, M,P-xylenes are not chromatographically separable. However, using a HRMS will differentiate the identifications.
Super xplanation
thsnk you so much, very helpful
Good information
Amazing
I really appreciate it
Thanks bro ❤
Thank you!
You said temp of injection is higher than column but column temp is 150 to 300° Celsius
Yes
Column temperature ka nahi ,column jisme placed hai na thermostat m uska temp hota hai 150-300
The injection temp is 15-20 times higher than the column. So injection port is 170-320°C
@@anushkajain6200speak in english no one understands indian
@@lion-o848we simply don't care whether you understand Hindi or not. There's a translation option underneath the comment if you want the comment in English.
Great vid. Thank you
Nice video
Excellent. Thanks for that.
Luv and Peace.
Nice.. Keep go head...
2:45 I don't understand when the temperature of the injection in 20-50 degrees Celcius and the column is 150-300 celcius which means the column is higher, but you said and is given in the picture that the temperature of the injection is higher than the column, does that mean the temperature of the injection is 150+20 degrees celcius? where we add that 20 and 50 ? Please can I get an explanation🥺
No temp of injection always greater than oven temperature
@@qualitycontrolguru then why does it say 20 to 50 degrees celcius? and the oven a huge number ? thank you for your reply :3
Highly useful
Why do you have music playing in the background?
My teacher says that GC is rapid faster than others (Column chromatography and HPLC) is that correct ?
Yes. Analysis is done within minutes or even sec.
Very useful
Understood easily ❣️
Wow explained well