Affinity chromatography

Affinity chromatography is a chromatographic technique in which separation occurs, based on the specific interaction between two molecules. This specific interaction can be between enzyme and substrate, receptor and ligand, protein and nucleic acid etc. affinity chromatography is usually carried out in column which is filled with the supporting material or the matrix. The matrix used should be chemically inert, should exhibit good flow properties and should have sufficient functional groups for the covalent binding of the ligand. The matrix is attached with a spacer arm which is usually made up of CH2 group. The presence of spacer prevents the non-specific interaction of the ligand with the matrix itself. when the mixture of different molecules is passed through the affinity column, only the molecule that have specific affinity for the ligand, binds the lignd. the other molecules are washed away. Next to dissociate the molecule of interest from the ligand, a buffer with different ionic strength of pH is passed. This finally releases the molecule of interest from the ligand and is obtained in the pure form.

[@MustafaAHadid]

so simple yet so informative. Thanks

[@SyenPie]

FANTASTIC video!! The animated diagrams were INCREDIBLY helpful, and I love how CONCISE and efficient the explanation was! I was struggling to understand this separation technique, but your video has helped tremendously.

[@suryashakthi4942]

❤️

[@kobedierckx2918]

Thank you, this video helped me with the preparation of my lab session.

[@spanditaroy9973]

Well explained

[@deivibarci2093]

Thanks a lot :))) It really helped me understand the whole lecture.

[@ranjinihj4303]

Thank you....Now I clearly understand...

[@alaleh3641]

Thank you it was really helpfull👌

[@kalaiselviprabhakar9186]

It is very useful bro.... Thanks 🙏🙏🙏 and one thing tomorrow is my exam

[@celebritystylewatch]

Amazing video, diagrams are perfectly repersenting the concept of the topic

[@priyankayadavvv]

Wowww, made it so easy to understand. Better than any other book explanation. Big thank you.

[@durgamthirupathi1838]

Thanks a lot

[@jameelasarwan1278]

Best of among all

[@citradca]

well explained thank you

[@martijov2475]

Great video!

[@shivaniadityasinha3591]

Thank you..it was really helpful

[@WaheedKhan128us]

Thanks

[@mr.perfect1744]

Thanks

[@tjp1612]

Kidu

[@mugadasaitilak2691]

a competing agent is used to displace the analyte from the affinity ligand?

[@hebat.6391]

Thanks bestie that was so easy to understand :)

[@jlancepolk]

Amazing! Was looking for a short and sweet vid and this did it! Thanks.

[@AmiraAmira-dc2ck]

It very easy to understand.thank you

[@padmabhushanachary9949]

Nice.....thank you...

[@naveenpp578]

Matrix and stationary phase are same ??😢

[@kashafimran325]

Easiest nd very informative thanku

[@traywaters1575]

This is literally the best video on this topic. Has helped me so much for my test tomorrow!

[@saeedanwar1394]

Great job 👍

[@AX-fc5sc]

thank you! it helped!:)

[@vaibhavalave988]

Please explain anion and cation chromatography

[@ankithanayak225]

Tq it is very helpful ❤️

[@valeriegonzalez7296]

thank u, u rock!

[@Coverworld-t3y]

Good job 👍

[@HammadAfzal-ux7vh]

If the sample molecules bind with the ligand then what is the function of matrix?
Is it used to support the ligand in the column?

[@nimramukthar1721]

very easy to understand

[@YeomraX]

thank u india

[@b.bhoomika0543]

Helpful

[@vivekkumarclasses6346]

Thanks

[@km2052]

thx

[@bts_rollercosterlifepurplearmy]

Animation makes concept effective..... 👍👍👍

[@marwamwita2908]

Bravooooo

[@beosayl6724]

LEGEND

[@AkilaChamikaraakiller]

එල මල්ලි උබ නම් දෙයියෙක්

[@payalhazarika4164]

I wish everything was so simply explained! Well educated. Thank you so much, it helped a lot!❤

[@Chemlord]

Great video...only the charge on Nickel hurted my eyes.

[@aknguven6408]

Amazing video:)))

[@fatimailyas610]

That's amazing ...keep it up ✨

[@aqibmehmood3011]

Awesome

[@ThePigeonBrain]

great explanation, thank you

[@vasuvasuu7667]

Thanks this video

[@PrithihshaRavi]

👏