SDS-PAGE explained - Protein Separation Technique
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- Опубликовано: 31 янв 2021
- Hey Friends,
SDS-PAGE (Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis) is used to separate proteins in a sample according to their molecular weight.
SDS binds to linearised proteins and masks the native charge. 1.4 g SDS bind per 1g protein, so that all proteins have a nearly similar mass-to-charge ratio.
After an SDS-PAGE, the gel is frequently used for Western Blot analysis to confirm the presence of a specific protein of interest.
Western Blot:
• Western Blot / Protein...
Here is a helpful explanation on SDS-PAGE:
ruo.mbl.co.jp/bio/e/support/m...
Thanks & Bye
Henrik 
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wow, after each video, i ask myself "why professors can not explain it that simply", thanks a lot!
Are we related? 👀
exactly that I'm Greek I study in Greek university and it s easier for me to learn all this in English than our notes that she gave us its not make sense she put so much useless details and they lost the mane subjects thanks for every one that really want to teach and not go to just do their job take the money and go
Because they have to pass the time
Many professors are researchers first and foremost, but I agree it’s definitely an issue. They may be excellent mentors and teachers to their grad student researchers, but when asked to explain basic principles of biology to a class of undergraduates, they often falter. This is because 1) professors do not receive formal training as educators at the K-12 levels do (absurd right?!) and 2) there is no incentive to go out and seek that training in their own time. Professors are made to wear many hats, and the most important and pressing one is obtaining grant money to fund their research. The good professors people have at university are often those who either did seek some sort of formal training during their PhD/postdoc or who just have a knack for presenting complex concepts. What’s that mean saying? “Those who can’t do, teach.” Well at university it’s the inverse: “Those who CAN do, [often] suck at teaching.” 😆
at this point , i think the prof don't understand the process, if you can't explain it this simply as in this video, then at least give us link to videos that explains it better to make our life easier
Clear & concise. Well-done!
Excellent material! This is a great job explaining and illustrating the topic. Very clear information.
Thanks for making these nice videos. They’re very clear and the diagrams/animations are really helpful
What a brilliantly concise explanation, thank you.
I watch all the ads for Henrik. He’s been very helpful.
Ahahahaha
bro this video is so good and so easy to understand thanks a ton dude
Such a wow video should be appreciated. Thanks for sharing this video
thank you so much, you helped me a lot with my lab report!!
this is the best SDS-Page explanation on the entire internet, thank you
I like this tutorial much. Very hepful in my current research project on p38 protein kinase
Very accurate and clear explanation. Thank you
U explaining so clearly thank you 🙏
You make the most informative and easy to understand videos. Thank you :D
your video was so helpful!!! thank u very much❤
amazing video and explanation, thanks hoss
I want to become a teacher which explains clear and easy like u!!!
Thank you so much 🙏🏽
👏👏Wellstone. Well explained
You just made my day useful
Well, that´s nice to hear!
Your videos are absolutely amazing
Please I didn’t see video about southern and northern blotting
Great channel
Super nice video!!! ❤
thank you very much!
Perfect Video!
Very well made video
Thank you very much you just save my marks for viva
Amazing!
Thank you professor
thank you so much amazing
Wonderful ❤
But how do we take the separated protein out of the gel? or we cannot?
thank you!
What site did you use to produce that sds image with the bands
I’m doing my dissertation on cspg4 protein and have ran sds page for analysis and would like a neat version like yours on your animation as an image to my real life image
Microsoft Powerpoint
God bless u
Danke!!!!
thank uuu for the videoo
Can you turn on subtitles?
good shit
can you use this method to find protein protein interactions?
This technique is part of another method that can be used for that:
Immunoprecipitation... you direct an antibody against the protein of interest (want to find out the interaction partners of that protein)... you isolate this protein and everything that is binding to it. Then you can run SDS PAGE to see the size of the proteins you have pulled down with your target.
@@henrikslab thank you so much for your explanation!
but why cant we use a normal gel elektrofores for it. why must the protien be unfolded
If the proteins are unfolded it is difficult to sort them accoriding to their size (in kD)... Why?
Because some proteins may folded like a tightly packed ball whereas others have a longer structure... to make that equal unfolding is important. Apart from that, the unfolding is essential to mask the charges (this would not be possible when they are tightly packed).
min 1:48 i cannot understand what he says:
having the equal _______
help please
"the equal mass to charge ratio"
Wht does buffer soln contain.?
Usually, for SDS one uses the TRIS-GLYCIN BUFFER:
It contains Tris, Glycin and SDS (0.1%)
@@henrikslab and u didnt mention about bromophenol blue..glycerol..gel preparation...or is there any another video?can u help me?
Very well-explained! Only comment/suggestion I have is that the negative terminal at the top is the anode, which is the negative terminal. The cathode is the positive terminal. The positive and negative terminals are labelled properly but the terms should be flipped in terms of their positions. Keep up the good wor!k!
This is the case for a galvanic cell it is reversed in electrophoresis, the positive pole is the anode. It is confusing but below is the explanation.
In a galvanic cell, the anode is (-), and the focus is on pushing away electrons from the anode to supply power. In an electrolytic cell and gel electrophoresis, the anode is (+) and the focus is on attracting the negative ions in solution from inputted power.
After watching this... i thought why my professor has to make every simple things present in a such complicated manner...why...
In many cases, because they were never really taught how to teach...
I love you ❤❤
Du könntest ein bisschen an deinem deutschen Akzent arbeiten :P Aber super Video!!! vielen Dank!!
you need to adjust the content
Low Voice
Isn’t anode negative and Cathode positive
لي جا من عند معماش يخبط جام 😂😂😂
I've seen some buffoons that couldnt explain in 30 mins. But all bro neded was 4 mins
deutsches voiceover wäre auch nice :)
würde Sinn machen, es gibt nämlich kein vernünftiges Vid auf deutsch
In der Wissenschaft sollte man aber schon Englisch können ^^
Sei doch froh dass du gleich bisschen scientific English lernst. Hilft auf jeden Fall wenn du mal vorhast papers zu lesen.